فهرست مطالب

Molecular and Clinical Microbiology - Volume:9 Issue: 2, 2019
  • Volume:9 Issue: 2, 2019
  • تاریخ انتشار: 1398/09/10
  • تعداد عناوین: 6
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  • Monir Aghamoosa, Azar Sabokbar *, Sepideh Arbabi Bidgoli, Naser Harzandi, Solmaz Ghaffari Pages 1150-1158
    Silver nanoparticles (AgNPs) are one of the most important nanoparticles which have various biomedical applications. For example as antifungal, antibacterial, anti-cancer and anti-inflammatory agents. Skin infection caused by Trichophyton rubrum and some opportunistic fungi such as Candida albicans and Aspergillus fumigatus are sometimes difficult to be treat. Although silver nanoparticle has long been used as effective inorganic antifungal agent; the antifungal activity of nano-Ag in different size has not been investigated yet. Anti-cancer and antifungal effects of spherical silver nanoparticles (nano-Ag) were investigated in this study and we decided to determination toxicity of Nano-Ag in different diameters (10, 20 and 40 nm) on L929 mouse fibroblasts. TEM microscope has been used to evaluate nanoparticles size and morphology. Nano-Ag’s toxicity were evaluated by MTT assay [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide]. This study showed that the toxicity of Nano-Ag in low concentration (16-32µg/ml) are sustainable and Silver nanoparticle effects are size dependent.
    Keywords: MTT Assay, Antifungal, viability, new drug, multi –resistant
  • Zohreh Akbarpour, Elham Moazamian * Pages 1159-1168
    Acinetobacter resistant strains have caused medical problems throughout the world. The aim of this study was to evaluate the frequency and pattern of antibiotic resistance of Acinetobacter species isolated from traumatic patients in Shahid Rajaee hospital in Shiraz. In this study, 794 samples were isolated from patients in Shahid Rajaee Hospital. Identification of Acinetobacter was done by biochemical tests and PCR method. Multi-drug resistant (MDR) Acinetobacter were determined using gentamicin, piperacillin, meropenem, colistin, trimethoprim, ciprofloxacin, imipenem, ampicillin, and chloramphenicol antibiotics. In this study, 248 samples of Acinetobacter isolates were identified by molecular and biochemical methods from patients. All of which were isolates of MDR Acinetobacter. The highest percentage of Acinetobacter isolates was reported for upper respiratory tract samples and the lowest for urinary tract. The highest percentage of infection was related to Acinetobacter co-infection with one bacterium in patients aged 45 to 87 years old. As the number of male patients with accident trauma was more than women, the percentage is higher in men. The percentage of patients with Acinetobacter infection in ICUs was higher than in other sections. These results show the evidence of necessity to examine the transmission ways and the increasing incidence of hospital infections.
    Keywords: Nosocomial Infection, Acinetobacter, Traumatic patients, Muti Drug Resistance, Antibiotic resistance
  • Mojtaba Taghizade * Pages 1169-1173
    Ear infection is one of the most common types of infections that can caused by fungal and bacterial agents. The precise diagnosis of the disease and identification of microbial agents is very important to prescribe the right drug and cure patients as soon as possible. The present study was to find the consistency of clinical findings and laboratory tests among patients suspected of ear infections over a one-year period. In this cross-sectional study conducted in 2018 to 2019, 134 ear samples of patients referred to the ENT clinic of Ayatollah Rouhani hospital of Babol were collected. These samples were analyzed and microbial agents were identified by direct examination and culture. The results were compared with an initial diagnosis from a physician. Out of the 84 patients clinically diagnosed for fungal infection, 67 cases (79.8%) were laboratory-approved, while the rate of bacterial- infection was 33 cases (66%). Our findings showed that the initial diagnosis of from a physician along with laboratory tests is necessary for accurate diagnosis and treatment of ear infections.
    Keywords: otitis, fungal, bacterial, LAB, mix
  • Mohadese Ramezani, Masood Ghane * Pages 1174-1180
    Urinary tract infection is the most prevalent infection in the human societies. This study aims to identify G. vaginalis in the urinary samples of the patients suffered from UTI by culture and PCR techniques. 200 patients with urinary tract infection signs have entered in this study. urine sample was cultured in the blood agar medium, a small quantity of the sample used for the purpose of molecular technique. In order to analyze the data, SPSS software and chi square (x2) test has been used. Of 200 studied patients, G. vaginalis was isolated and identified in 6 (3%) and 29 (14.5%) patients with the culture and PCR techniques, respectively. The sequencing of the samples confirmed phenotyping identification. On the basis of the results obtained from both two techniques, it can be expressed that PCR technique is more appropriate to identify G. vaginalis. The achieved results show that G. vaginalis is regarded as one the agents causing the occurrence of urine infection. Yet, in many medical diagnosis laboratories, the bacterium is undetectable due to hardly growth. Also, considering the conducted techniques, PCR method is of a higher accuracy and speed.
    Keywords: identification, Urinary Tract Infection, Culture Techniques, Molecular Technique, Gardenerlla vaginalis
  • Elmira Behrouzpour, Kumarss Amini * Pages 1181-1187
    L-Glutaminase is a therapeutic enzyme found in various microbial source have been considered in the cancer therapy. Sampling was carried out from the shores of the Persian Gulf. After identifying and performing specific biochemical tests, marine Streptomyces was isolated and DNA extraction was performed. Through the PCR test, the strains of streptomycin with the L-glutamine enzyme gene were identified. The L-gluta gene was positively transmitted to the host bacterium Escherichia coli via a vector and cloned through the TA technique, and the Real Time PCR technique was used to measure the expression of genes in E. coli origami. The software clustalX and Mega5 were used to draw the phylogenetic tree. Out of 12 Streptomyces isolates, 58.3% of isolates were carried L-gluta gene. After cloning the L-glutaminase gene by colony selection (blue / white), the cloned strains were isolated. The real-time PCR test showed a successful expression of the L-gluta gene on the cloned strains. Phylogenetic results with the neighbor joining (NJ) method show that, Streptomyces species with bootstrap values 99% located in a clade which indicated their close relatedness. The results of this study showed that the Persian Gulf is one of the high potential sources with the production of secondary metabolites and useful antimicrobial products that can be used as a useful source of various biological products such as L-glutaminase.
    Keywords: Streptomyces, cloning, expression, L-glutaminase, Persian Gulf
  • Parisa Maddahi, Sevil Nematollahi * Pages 1188-1196
    Fusarium oxysporum f.sp. lycopersici is an important disease agent of tomato which causes wilt and seedling. The present study was performed to evaluate the antifungal effect of Achillea millefolium, Salvia verticillata and Ziziphora clinopodioides extracts and their abilities to inhibit the fungus. For this, methanol extracts of reference plants was extracted and tested in concentrations ranging from 1 , 1.5 and 2 mg/ml on mycelial growth of Fusarium oxysporum. The same extracts were then tested for antifungal activity in vivo in the greenhouse on inoculated tomato plants. Z. clinopodioides demonstrated highest antifungal activity against mycelial growth of F. oxysporum strain that recorded 77.1%, 62.03% and 61.99% at 2, 1.5 and 1 (mg/ml), respectively. the MIC value for of Z. clinopodioides against F. oxysporum was 3.125 mg/ml followed A. millefolium and S. verticillata extract having 6.25 mg/ml. The MFC of extracts was found to be 6.25 mg/ml in Z. clinopodioides and 12.5 mg/ml for A. millefolium and S. verticillate. In greenhouse experiment employing methanol extracts of three plant species showed an increase in the mean plant height and also fresh and dry weight of root and shoot with the consequent reduction in the disease symptoms of the tomato seedlings. Overall, the results showed significant growth inhibition activity of Z. clinopodioides methanol extract against F. oxysporum in both in vitro and greenhouse condition. Although the extracts of A. millefolium and S. verticillata which had no effect in vitro assays, in greenhouse conditions, these plants showed considerable antifungal activity.
    Keywords: Biological control, Disease management, Fungal growth, F. oxysporum, Medicinal plant