فهرست مطالب

  • Volume:14 Issue: 2, 2020
  • تاریخ انتشار: 1399/05/06
  • تعداد عناوین: 10
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  • Kuei-ling Hsu, Abdulrahman A. Balhaddad, Isadora Martini Garcia, Fabricio Mezzomo Collares, Louis DePaola, Mary Anne Melo* Pages 77-82
    Background

     The disinfection of orthodontic acrylic resins might change the physical and mechanicalproperties of these materials. We aimed to investigate the impact of four different commercially availabledisinfectants on the surface roughness of acrylic resins used for orthodontic appliances.

    Methods

     Four disinfectant solutions (BirexSE, Opti-Cide3, COEfect MinuteSpray, and CaviCideSpray) were used to disinfect orthodontic acrylic resins using the spraying method. The resins weresubjected to repeated disinfection protocols. Distilled water, also applied via spraying method, was usedas a control. Surface roughness was scrutinized to examine the extent of surface topography changes bystylus profilometry. Data normality was evaluated via the Shapiro–Wilk test, followed by the WilcoxonSigned-Rank test for non-parametric data or paired Student’s t-test for parametric data to compareintra-group differences in roughness before and after the use of the disinfectant solutions.

    Results

     Some of the disinfectants (BirexSE and CaviCide) resulted in significant changes in surfaceroughness values before and after the disinfection compared to the controls (P<0.05). The groups thatwere in contact with distilled water, Opti-Cide, and Coeffect did not exhibit significant differences insurface roughness before and after the intervention (P>0.05). However, from a clinical perspective, theresulting variations in surface roughness (<%0.15) induced by these solutions might not reflect clinicallysignificant differences.

    Conclusion

    The use of disinfectant solutions is unlikely to harm the surface of orthodontic acrylic resins.Oral care providers need to be attentive to the interpretation and implementation of clinically significantchanges in their evidence-based approach regarding potential material damages by disinfection sprays.

    Keywords: Acrylic resin, Disinfection, Orthodontic, Surface roughness
  • Shiva Shankar Gummaluri*, Hirak S Bhattacharya, Madhusudan Astekar, Shivani Cheruvu Pages 83-91
    Background

    Various treatment modalities, such as leucocyte platelet-rich fibrin (L-PRF), bone grafts,and membranes, have been used for the restoration of lost periodontal tissues. Titanium-preparedplatelet-rich fibrin (T-PRF) has attracted attention for its proper haemocompatibility, thick fibrinmeshwork, and long resorption time. The present study aimed to evaluate the effectiveness of T-PRFand L-PRF in the management of intra-bony defects based on clinical and radiographic criteria.

    Methods

     Twenty-six subjects with 34 intra-bony 3- walled defects were divided into two groups (n=17)and treated with T-PRF or L-PRF. Clinical and radiographic measurements were recorded at baselineand 6- , 3- and 9- month intervals and tabulated on Microsoft Excel spreadsheets. For intra- and intergroupcomparisons, paired and unpaired t-tests were performed. P<0.05 was set as statistically significant

    Results

    Intra-group comparisons revealed statistically significant differences (P<0.05) from baseline inboth groups regarding clinical measurements. On intergroup comparison, the T-PRF group exhibited asignificantly higher defect fill compared to the L-PRF group (P<0.05).

    Conclusion

     Within the limits of the present study, T-PRF seems to be a better alternative to L-PRF inthe treatment of intra-bony defects.

    Keywords: Bone regeneration, Chronic periodontitis, Periodontal pocket, Debridement, Platelet-rich fibrin
  • Praveen Kumar Neela*, Srinivas Reddy Gosla, Akhter Husain, Vasavi Mohan, Sravya Thumoju, Rajeshwari BV Pages 93-96
    Background

     Cleft lip and palate (CLP) is a common congenital anomaly. Many genes, like MAPK4 andSOX-1OT, are associated with its etiology in different populations. High-risk markers on these genesreported in other populations were not studied in our population. Hence, the study aimed to determinethe association of MAPK4 and SOX-1OT polymorphisms in CLP in multiplex families.

    Methods

     Based on inclusion and exclusion criteria, we selected 20 multiplex CLP families for thiscase‒control study, in which the affected individuals and healthy controls selected from these familieswere compared. Fifty subjects affected with cleft and 38 unaffected subjects were included in the study.The polymorphisms studied for the association consisted of rs726455 and rs2969972 in the genes SOX-1OT and MAPK4, respectively. DNA was isolated and sent for genotyping using the MassArray method.Plink, a whole-genome association analysis toolset, was used for statistical analysis.

    Results

     Both polymorphisms followed Hardy–Weinberg equilibrium. The rs726455 of SOX-1OTyielded a P-value of 0.983 and an allelic odds ratio (OR) of 0.983. For rs2969972 of MAPK4, the P-valuewas 0.04 (significant), and the allelic OR was 0.51. Minor allele frequency (MAF) in the unaffectedsubjects was more than the MAF in the affected subjects for rs2969972.

    Conclusion

     The results suggested that polymorphism rs726455 on SOX-1OT was not associated withfamilial cases of CLP. Since MAF in the unaffected subjects was more than the MAF-affected subjects,rs2969972 on MAPK4 is protective in the multiplex families.

    Keywords: Cleft lip palate, Gene, Polymorphism
  • Marzie Aghazade, Mohammad Samiei, Marjan Imani, Zahra Aghazadeh, Effat Alizadeh, Fereshte Rezaie* Pages 97-103
    Background

     Stem cell-based treatment modalities have been potential strategies for tissue regenerationin many conditions. Several studies have evaluated the biologic properties of DPSCs and their efficacyin the treatment of a variety of diseases. The present study was undertaken to evaluate the adhesionbehavior of DPSCs on different endodontic materials before and after setting.

    Methods

     The crowns of the selected teeth were removed, and the root canals were prepared andobturated with gutta-percha and AH26 sealer. A retrograde cavity was prepared at root ends. Differentmaterials were placed in the cavities. Then the samples were attached to the wells with the use of achemical glue. Dental pulp stem cells were allowed to proliferate to reach a count of 2 million andtransferred to -12well plates in association with a culture medium. Finally, the samples attached to thewells were exposed to the stem cells immersed in the culture medium before and after setting. Thenadhesion of the stem cells was evaluated using SEM.

    Results

     The SEM results showed cellular adhesion in the samples containing CEM cement both beforeand after setting. The samples containing MTA Angelus and ProRoot MTA exhibited cellular adhesionbefore setting, with no cellular adhesion after setting. The samples containing AH26 and MTA Fillapexsealers exhibited cellular adhesion after setting, with no adhesion before setting. The samples containingsimvastatin exhibited no cellular adhesion before setting; this material had dissolved in the culturemedium after setting evaluation.

    Conclusion

    The results of the present study showed that of all the materials tested, CEM cement hadthe highest capacity for dental pulp stem cell adhesion.

    Keywords: Adult stem cells, Biocompatible Materials, MTA, Regenerative Endodontics
  • Narmin Mohammadi, Soodabeh Kimyai*, Yasaman Ghavami Lahij, Mahmoud Bahari, Amir Ahmad Ajami, Mahdi Abed Kahnamouei, Mehdi Daneshpooy Pages 105-109
    Background

     The use of bleaching agents might result in microstructural changes in tooth structure andin restorative materials. This study compared the effects of bleaching with %15 carbamide peroxide and%35 hydrogen peroxide on the flexural strength of Cention N restorative material using the self-curedand dual-cured polymerization modes.

    Methods

    Sixty bar-shaped samples of Cention N restorative material were included in this in vitrostudy and assigned to three groups (n=20) randomly: control, bleaching with %15 carbamide peroxideand bleaching with %35 hydrogen peroxide. Each group was divided into two subgroups: samplespolymerized in the self-cured mode and samples polymerized in the dual-cured mode. Then the flexuralstrengths of the samples were determined. Two-way ANOVA was used to compare flexural strengthsbetween the three groups in two polymerization modes, followed by post hoc Tukey test. Statisticalsignificance was defined at P<0.05.

    Results

    The difference in the mean flexural strength was significant in terms of the bleaching regimen(P<0.001), with significantly lower flexural strength in the two bleaching groups compared to thecontrol group. However, the mean flexural strengths were not significantly different in terms of thepolymerization mode applied (P=0.14).

    Conclusion

     The application of %15 carbamide peroxide and %35 hydrogen peroxide bleaching agentsdecreased the flexural strength of Cention N restorative material. Irrespective of the bleaching regimen,there was no significant difference in the flexural strength of Cention N between the self-curing anddual-curing polymerization modes.

    Keywords: Composite resins, Dental materials, Flexural strength, Tooth bleaching, Tooth bleaching agents
  • Gülsah Uslu*, Mustafa Gündoğar, Taha Özyürek, Gianluca Plotino Pages 111-115
    Background

     This study was conducted to compare the cyclic fatigue resistance of VDW.ROTATE,TruNatomy Prime, HyFlex CM, and 2Shape nickel-titanium (NiTi) rotary instruments in doublecurvedcanals in a simulated clinical environment.

    Methods

     Eighty NiTi files were used for the fatigue testing in stainless steel canals compatible withinstrument sizes until fracture occurred (n=20): VDW.ROTATE (04./25#), TruNatomy Prime (04./26#),HyFlex CM (04./25#) and 2Shape TS04./25#( 1). For each instrument, the number of cycles to fracture(NCF) was calculated, and the fractured fragment length (FL) was measured. To verify that the fileswere fractured due to cyclic fatigue, the fractured surfaces of the files were evaluated under a scanningelectron microscope. Data were statistically analyzed using the Kruskal–Wallis and Student’s t-tests atthe %95 confidence level.

    Results

     The failure of the files due to cyclic fatigue was first seen in the apical curvature before thecoronal curvature (P<0.05). The highest fatigue resistance was observed in VDW.ROTATE and HyFlexCM files in both curvatures (P<0.05). There were no significant differences in the fatigue resistancebetween the HyFlex CM and VDW.ROTATE files or between the 2Shape and the TruNatomy files(P>0.05). There was no difference in the fractured lengths of the files between the apical and coronalcurvatures (P>0.05).

    Conclusion

    In artificial S-shaped root canals, VDW.ROTATE and HyFlex CM files exhibited higherfatigue resistance compared to 2Shape and TruNatomy files.

    Keywords: Double curvature, Fatigue resistance, Nickel-titanium, Rotary instruments
  • Shazeena Qaiser*, Mithra N. Hegde, Darshana Devadiga, Mahalaxmi Yelapure Pages 117-123
    Background

     Bioceramics need to interact chemically with dentin to exhibit adequate bioactivity.Proper bonding of bioceramics with dentin is of considerable importance. This study aimed to evaluatethe wettability and marginal adaptation of bioceramics after the use of surface-active agents on dentin:%0.5 cetrimide and %1 alkylbenzene sulfonate.

    Methods

     Of ninety maxillary central incisors, 45 teeth were sectioned longitudinally with 45 roothalves randomly assigned to three groups (n=15): group I: no pre-treatment; group II: %0.5 cetrimide;group III: %1 alkylbenzene sulfonate. Then, the samples were subdivided into three subgroups (n=5):subgroup I: MTA; subgroup II: Biodentine, and subgroup III: BioRoot. A controlled-volume dropletof bioceramic material was placed on each root half, which was positioned in a dynamic contact angleanalyzer to record the static contact angle for wettability. The remaining 45 samples were decoronated;the root canals were prepared and randomly categorized, as mentioned above. The root canal surfaceswere treated, filled with the bioceramic material, transversely sectioned, and then each middle sectionwas analyzed microscopically for marginal adaptation. Statistical tests used included post hoc Tukeytests and one-way ANOVA. The level of statistical significance set at %95 (P<0.05).

    Results

     The contact angle values and interfacial gap width values after surface pre-treatment weresignificantly lower when compared to no pre-treatment group (P<0.05). The values were significant for%0.5 cetrimide in the case of Biodentine and %1 alkylbenzene sulfonate in the case of BioRoot (P<0.05).

    Conclusion

    The two surfactants yielded promising results for enhancing the wettability and marginaladaptation of materials to the root dentin, which is required for obtaining an adequate seal, penetration,and bond strength of bioceramics.

    Keywords: Bioceramic, Surfactants, bonding, Wettability, Marginal adaptation
  • Pelin Tufenkci*, Aylin Kalaycı ORCID Pages 125-129
    Background

    This study aimed to assess the accuracy of three electronic apex locators (EALs) (DentaportZX [J Morita, Tokyo, Japan], Propex Pixi [Dentsply Maillefer, Ballaigues, Switzerland], and iPex II [NSK,Tokyo, Japan]) during root canal retreatment.

    Methods

     The root canal lengths of 90 extracted single-rooted human teeth were determined under adental operating microscope at ×10 magnification. The actual working length (AWL) was 0.5 mm lessthan the root length. Electronic measurements were performed with the three EALs. The root canalswere instrumented and filled to the actual working length using the lateral compaction technique. Afterseven days, the teeth were retreated until the retreatment file was applied to the root canal at the workinglength determined by EALs, and then, the three EALs were used for determining the retreatmentworking length. Data were analyzed using chi-squared and Kruskal–Wallis tests.

    Results

     In the retreatment, the accuracy of EALs was reported at %83.3 for Dentaport ZX, %83.4 forPropex Pixi, and %80 for iPex II within a tolerance of 0.5± mm of the AWL.

    Conclusion

    Under the limitations of this study, Dentaport ZX, Propex Pixi, and iPex II can be a usefuladjunct during retreatment. Clinicians should be aware that residual materials in the root canal duringretreatment can affect the accuracy of EALs.

    Keywords: Electronic apex locator, Retreatment, Working length
  • Esra Bolat*, Elçin Esenlik, Meral Öncü, Meltem Özgöçmen, Mustafa Cihat Avunduk, Özlem Yüksel Pages 131-137
    Background

     This experimental study aimed to assess the effects of Vitamins C and E on orthodontictooth movement.

    Methods

    Fifty-one male Wistar albino rats were divided into six groups: five appliance groups and onecontrol group. The appliance groups had an orthodontic appliance consisting of a closed-coil springligated between the maxillary incisor and maxillary first molar (50 g). Vitamin E and C (150 mg/kg)were injected intraperitoneally per day in the first and second groups, respectively. Vitamins E andC (20 μL) were locally injected into the periodontal gap of the moving teeth in the third and fourthgroups, respectively, once every three days. No vitamin was injected in the last (fifth) appliance group.The experimental period was 18 days. Histological and biochemical (alkaline phosphatase, osteocalcin,and NTx levels) evaluations of the samples were performed, and maxillary incisor‒molar distance wasmeasured before and after the experiment.

    Results

     The amount of tooth movement was similar in the appliance groups. All the vitamin groupsshowed significantly increased osteoblastic activity, while those treated with systemic vitamins exhibitedsignificantly increased numbers of collagen fibers on the tension side compared to the appliance controlgroup (P<0.05).

    Conclusion

     Vitamin C and E supplements positively affected bone formation on the tension side of theteeth during experimental orthodontic tooth movement.

    Keywords: Histomorphometry, Tooth movement, Vitamin C, Vitamin E
  • Numaan Nisar, Kumar Nilesh*, Mushtaq Ishaq Parkar, Prashant Punde Pages 139-145
    Background

     Alveolar bone remodeling after tooth loss results in reduced ridge dimensions in horizontaland vertical planes. To prevent this, various authors have proposed different ridge preservationtechniques. A collagen plug is a novel material that has shown promising results in preserving thealveolar bone. PRP has also yielded favorable outcomes in wound healing and promoted osteoinductionand osteoconduction

    Methods

    Thirty patients of both sexes with an age range of 30–18 years requiring bilateral extractionof teeth with similar tooth root anatomy in the maxilla or mandible were included in the study. Theextraction of teeth was carried out atraumatically. The patients’ arches were randomly divided andlabeled as the test or control sides. Bone width was measured on both sides. A collagen plug, with PRP,was placed, and the extraction socket was sutured on the test side. The control side was just sutured. Abaseline RVG was taken to record the apico-coronal height. The patients were recalled after 10 days forsuture removal and evaluation of wound healing. Parameters were re-evaluated at three and six monthspostoperatively. The data were subjected to t-test and one-way ANOVA.

    Results

     The height of the crestal bone on the grafted side was more when compared to the non-graftedside three and six months after tooth extractions, and the difference was statically significant (P<0.001).No statistically significant difference was seen in the width of the alveolar bone three and six monthsafter tooth extraction (P>0.05).

    Conclusion

    Collagen and PRP provided reasonable socket preservation as simple and inexpensiveoptions as compared to other materials.

    Keywords: Alveolar ridge, Collagen, Graft, Platelet-rich plasma