فهرست مطالب

International journal of basic science in medicine
Volume:5 Issue: 2, Jun 2020

  • تاریخ انتشار: 1399/07/16
  • تعداد عناوین: 6
|
  • Ali Akbari, Mohsen Moghadami, Younes Ghasemi, Mehrdad Sharifi, Abdolrasoul Hemmati, Abdolkhalegh Keshavarzi, Fatemeh Javanmardi, Amir Emami* Pages 33-38
    Introduction

    The emergence of severe acute respiratory syndrome coronavirus 2 (SARSCoV-2) has created a global concern for public health. Having sufficient data is the first step to understand the behavior of contagious diseases such as the COVID-19 pandemic. Although various studies have assessed COVID-19 features, there are many limitations about patients’ characteristics, complications, and outcomes in different countries including Iran. The present study reported launching an electronic database for COVID-19 patients in Fars province, Iran.

    Method

    A comprehensive web-based multicenter registry was designed and launched by Shiraz University of Medical Sciences in order to collect all information about COVID-19 hospitalized patients in Fars province, Iran.

    Results

    In this registry, patients’ demographic characteristics, chest computed tomography scan findings, laboratory tests, complications during hospitalization, treatments, and disease course in Intensive Care units are recorded on a web-based electronic database. The virtual statues of the patient’s family are evaluated by telephone calls, and the proceeding will be implemented for quarantine and hospitalization if required.

    Conclusion

    The registry is hoped to help all scientists to understand the current challenges and be prepared for possible future waves of the epidemic. Finally, this registry is a resource for all researchers who are interested in coronavirus and plays an important role in supporting the scientific community on the frontlines of combating the virus.

    Keywords: COVID-19, Severe acute respiratory syndrome coronavirus 2, Registry, Iran
  • Fahimeh Piryaei, Hossein Mozdarani*, Mohammad Ali Sadighi Gilani, Mansour Ebrahimi Pages 39-42
    Introduction

    MiRNA expression alterations are closely related to growth, proliferation, and cell development. In addition, miR-187 has been found to regulate cellular proliferation. Further, spermatogenesis is a highly complicated process which is regulated through several genes targeted by miRNAs. However, no study has completely addressed the etiology of spermatogenic impairments. Accordingly, the present study examined the expression pattern of Homo sapiens (human) microRNA (hsa-mir-187) in the testicular biopsies of infertile men with non-obstructive azoospermia (NOA) and Sertoli cell-only syndrome (SCOS) subtype in comparison with those with obstructive azoospermia (OA).

    Methods

    Quantitative reverse‐transcription polymerase chain reaction analysis was performed to assess the expression level of hsa-mir-187 using the ΔΔCt method.

    Results

    Our data indicated that hsa-mir-187 was significantly up-regulated in the testis of the SCOS group (P˂ 0.05) in comparison with the OA group.

    Conclusion

    In general, the findings suggest that the differential expression of hsa-mir-187 is linked with male infertility and shed more light on molecular defects that underlie spermatogenic impairments.

    Keywords: Non-obstructive azoospermia, Obstructive azoospermia, miR-187, Sertoli cell-onlysyndrome, Testicular tissue
  • Parisa Shahbazi, Mohammad Jahantigh*, Saeed Salari, Salehe Danesh Pages 43-47
    Introduction 

    The production of β-lactamase in bacteria, especially in Escherichia coli as a prevalent opportunistic bacterium, has caused many problems in patient treatment. β-lactamases are encoded by extended-spectrum β-lactamase (ESBL) genes such as blaTEM and blaCTX-M. We aimed to assess the prevalence and antibiotic sensitivity of β-lactamases encoded by blaCTX-M and blaTEM in E. coli isolated from patients suffering from urinary tract infections (UTIs).

    Methods 

    Escherichia coli strains were isolated from the patients’ urine culture presented to medical diagnostic laboratories in Zabol, Iran. The agar disc-diffusion test was performed on Müller-Hinton agar to investigate the antibiotic resistance of these isolates using eight antimicrobial paper discs including gentamicin, tetracycline, co-trimoxazole, norfloxacin, cefuroxime, ampicillin, neomycin, and amoxicillin. A conventional polymerase chain reaction (PCR) was used to detect blaCTX-M and blaTEM.

    Results 

    The frequencies of resistance to cefuroxime, norfloxacin, co-trimoxazole, neomycin, amoxicillin, tetracycline, gentamicin, and ampicillin were found to be 45 (90%), 15 (30%), 33 (66%), 33 (66%), 44 (88%), 34 (68%), 4 (8%), and 50 (100%), respectively. Moreover, the prevalence of blaCTX-M was 25 (50%) while that of blaTEM was 16 (32%).

    Conclusion

    Based on the results, gentamicin and norfloxacin can be recommended as effective antibacterials for treating UTI caused by E. coli in the study population. Moreover, the frequency of resistant genes including blaCTX-M and blaTEM was high in the isolated E. coli. Effective control systems including appropriate treatments for ESBL-producing strains are therefore required for humans and food animals.

    Keywords: ESBL, E. coli, Prevalence, Antimicrobial resistance
  • Tahmine Ostovar, Hosein Rezaei, Javad Zavar Reza* Pages 48-53
    Introduction

    Chronic kidney disease (CKD) is one of the most threatening and important disorders worldwide in both industrial and developing nations. In addition, neutrophil gelatinase-associated lipocalin (NGAL), liver-type fatty acid-binding protein (L-FABP), and kidney injury molecule-1 (KIM-1) are three factors suggested as diagnostic and prognostic biomarkers in CKDs. Considering the lack of enough efficiency of the creatinine in the prognosis of the CKD, the present study aimed to assess the relationship between these three factors and CKD occurrence and determine if they could be considered valid biomarkers in this regard.

    Materials and Methods

    The present case-control study was designed enrolling 42 patients with confirmed CKD referring to the Imam Khomeini hospital of Kangan. The participants were 42 years old and gender-matched healthy counterparts. Blood samples were obtained, and then NGAL, KIM-1, and L-FABP were determined by the enzyme-linked immunosorbent assay using commercial kits (Bioassay Technology Laboratory). Finally, the serum creatinine was detected by applying Jaffe’s method.

    Results

    Based on the results, significant differences were found in the serum levels of all four factors between CKD patients and the control group. More precisely, the serum levels of NGAL (P < 0.0001, specificity: 87.6%, sensitivity: 79.3%, and the area under the curve, AUC: 0.89), L-FABP (P < 0.0001, specificity: 83.3%, sensitivity: 78.3%, and AUC: 0.86), KIM-1 (P < 0.0001, specificity: 85.7%, sensitivity: 78.6%, and AUC: 0.88), and creatinine (P < 0.0001) were significantly higher in individuals with CKDs in comparison with controls. Eventually, the serum levels of NGAL, L-FABP, and KIM-1 were significantly correlated with each other in both patient and control groups (P < 0.0001).

    Conclusion

    In general, NGAL, L-FABP, KIM-1, and creatinine could be used as independent biomarkers for the diagnosis of CKD. Moreover, the measurement of NGAL, L-FABP, and KIM-1 altogether could be a valid assessment for the diagnosis of CKD.

    Keywords: Chronic kidney disease, Liver-type fatty acid-binding protein, Kidney injurymolecule-1, Neutrophil gelatinase-associated lipocalin
  • Katayoon Karimzadeh*, Mahdiyeh Ramzanpoor, Shadi Keihankhadiv Pages 54-60
    Introduction

    Seaweeds are valuable resources for the discovery of efficient and safe drugs for pain treatment. In the present investigation, we evaluated the antinociceptive and anti-inflammatory properties of, methanolic extract of Laurencia caspica, a red algae, in mice models.

    Methods

    The analgesic effect of methanolic extract of L. caspica was assessed by hot-plate and acetic acid-induced writhing tests in male Swiss albino mice (weight = 20-25 g). The anti-inflammatory activity of methanolic extract of L. caspica was also evaluated by formalin-induced ear edema and xylene-induced paw edema tests.

    Results

    The total flavonoid content of the extract was estimated as 0.0537 mg quercetin/g extract. Both first and second phases of the nociception were significantly inhibited at a dose of 120 mg/kg of methanolic extract of L. caspica. The observed anti-inflammatory effect was dose-dependent. Acetic acid-induced writhing test and hot plate test showed that the extract significantly reduced pain in all evaluated doses (15, 30, 60, and 120 mg/kg). The antinociceptive activity of the methanolic extract was significantly reduced by naloxone (4 mg/kg). Moreover, the extract significantly reduced paw edema at the dose of 120 mg/kg in all the animals.

    Conclusion

    Methanolic extract of L. caspica exhibited central analgesic effect, as well as anti-inflammatory activity probably due to the presence of constituents like flavonoids and triterpenoids.

    Keywords: Red algae, Laurencia caspica, Anti-inflammatory, Antinociceptive
  • Masumeh Jalalvand, Gholamreza Shahsavari, Ali Sheikhian, Ali GanjiGhasem Mosayeb* Pages 61-65
    Introduction

    Satureja khozestanica grows mainly in the southwest part of Iran as a native plant. This edible herb contains various compounds including the S. Khuzestanica essential oil (SKEO) and monoterpene known as Carvacrol. Previous studies have shown the anti-inflammatory effects of S. Khuzestanica without mentioning the exact mechanism of its function. Given that prostaglandin synthesis, which is one of the main mediators of inflammation, is regulated by the cyclooxygenase-2 (COX2) gene, the present study investigated the effects of SKEO and Carvacrol on the expression of the COX2 gene in the stimulated-J774A.1 macrophage cell line.

    Methods

    To this end, fresh aerial parts of the plant were processed to prepare SKEO. Then, different doses of SKEO and Carvacrol (i.e., 0.004%, 0.008%, and 0.016% v/v) were used to treat with the lipopolysaccharides (LPS)-stimulated cell line for eight hours. After RNA extraction, the real-time polymerase chain reaction technique was applied for gene expression analysis.

    Results

    In the LPS-stimulated J774A.1 macrophage cell line, COX2 gene expression reduced significantly in a dose-dependent manner (0.004%, 0.008%, and 0.016%, P = 0.024, P = 0.021, and P = 0.013 v/v of SKEO, respectively) by SKEO, and the effect of Carvacrol was less powerful (0.008% and 0.016%, P = 0.027 and P = 0.038 v/v, respectively) compared to SKEO. Finally, the comparison between SKEO and Carvacrol showed higher significant inhibitory effects of SKEO on COX2 gene expression in comparison with Carvacrol in 0.004% v/v concentration (P = 0.037).

    Conclusion

    In general, SKEO significantly reduced COX2 gene expression, thus it can be suggested that its anti-inflammatory effect may result from the inhibition of the synthesis of this pro-inflammatory gene.

    Keywords: Carvacrol, Satureja khuzestanica, Cyclooxygenase-2 gene