مجله علوم و صنایع غذایی ایران
سال هفدهم شماره 8 (پیاپی 105، آبان 1399)

Snacks have become a significant part of the diet of many people, especially children. These snacks have many fans due to their attractive objects and colors, high durability and relatively low cost. Most of them are made from cereals, and the main problem with these snacks is insufficient nutrients, which have a negative effect on health and increase obesity in people, especially children. Using quinoa grain in products, it creates diversity in current products and also increases the nutritional value of snack. On the other hand, today, attention has been paid to the re-use of by-product in the food industry and the rate of agriculture inputs, and returning these products to the production cycle will increase the value added. One of these by-product is suni bug damaged wheat, which is used not used in the cooking industry due to the damage of the gluten network and is consumed by livestock feed. In this study, the effect of the independent variables included the percentage of addition of quinoa (0.25%, 50%), moisture content (12, 15, 18%), the screw speed (120,160,200 rpm) was in the form of a central composite design. The influence of the independent variables on some physical and functional properties was investigated, including expansion ratio, hardness, water absorption index, water solubility index, sensory evaluation. The results showed that with increasing quinoa up t0 50%, increased the hardness (4.72-11.57N), water solubility index (21-28.4) and overall acceptability of the extruded product, and also decreased the ratio of expansion (3.77-2.86), water absorption index (5.39-5.05) and sensory acceptability of the texture. Sensory evaluation results showed that 12% humidity conditions, 175rpm screw speed and quinoa addition levels (19%) in the suni- bug wheat were suitable for snack production and had the highest overall acceptability.

Hyssopus officinalis and frankincense (Boswellia carteri), as valuable medicinal herbs, are widely used in traditional medicine. Due to the increased resistance of pathogenic microorganisms to antibiotics and increasing of treatment costs, attentions has been focused to compounds of natural origin. In this study, Hyssop and Frankincense oils were extracted separately by water distillation. The essential oils components were identified by GC/MS. Determination of inhibition zone diameter and minimum inhibitory concentration were performed by disk agar diffusion and macro dilution methods, respectively. Wells with no discoloration were used to detect the minimum bactericidal (fungicidal) concentration. In this research, 24 and 22 compounds were identified in Hyssop and Frankincense, respectively. The main component of Hyssop oil was cis-3-pinanone (%28.2), and the main component of Frankincense oil was α- pinene (%22). Both Hyssop and Frankincense oils had the highest effect on Staphylococcus aureus and Bacillus cereus, and the lowest growth zone diameter was related on Escherichia coli and Pseudomonas aeruginosa. Also, it was found that the Candida albicans was more sensitive than Aspergillus niger against both essential oils (p<0.05). The results showed that plants are rich in secondary products such as terpenoids, alkaloids and flavonoids, most of which have antimicrobial activity.

In the present study, pumpkin flour with 0, 1, 3 and 5% was used as a substitute for flour and sugar in biscuit formulations. The physicochemical, antioxidant, rheological, sensory properties and peroxide value of the samples during the intervals 1, 30 and 60 days after baking were examined. The results of rheological evaluation of dough showed that the lowest degree of dough softening and the highest quality number belonged to the treatment T3 (containing 5% pumpkin flour + 95% wheat flour + 25% sugar) (p≤0.05). By increasing the amount of pumpkin flour, the protein content of cooked biscuits significantly decreased and the fat, fiber and moisture content of biscuits significantly increased (p≤0.05). With the increase in the amount of pumpkin flour, the content of the peroxide value of biscuits significantly decreased. In all of the studied days, increasing the amount of pumpkin flour, the total phenol content of the samples increased significantly and the IC50 of the biscuits significantly decreased (p≤0.05). The results showed that adding different amounts of pumpkin flour caused a significant decrease in the hardness and cohesiveness of the samples (p≤0.05) The sensory evaluation results of the samples showed that the highest total acceptance rating belonged to the treatment T3 (containing 5% pumpkin flour + 95% wheat flour + 25% sugar), the treatment was introduced as superior treatment.

Gamma-aminobutyric acid (GABA) is a non-protein amino acid existing in bacteria, plants, and vertebrates. GABA is especially well-known because of its physiological role in the neurotransmission, induction of hypotension, diuresis, and tranquility. GABA is biologically synthesized by GABA-producing lactic acid bacteria (GLAB) which are widely used as starters in the fermented foods. In this study, GABA-producing strain were chosen to be microencapsulated by soy protein isolate (SPI)-alginate using emulsion method. Encapsulation efficiency and entrapment of GLAB into soy protein-alginate microcapsules (SAE) was confirmed by scanning electron microscope. The GABA-producing ability and survivability of the microencapsulated GLABs were investigated in the human gastro-intestinal simulant. For screening of GLAB strains, the isolates from Tarkhineh and fermented carrot were separately cultivated in MRS broth supplemented with 1% (w/v) monosodium glutamate (MSG). The GABA production efficiency was studied by thin layer chromatography (TLC) and High performance liquid chromatography (HPLC). According to the recorded chromatograms, Lactobacillus brevis PML1 isolated from Tarkhineh and Lactobacillus brevis G42 from fermented carrot showed GABA producing ability of 304 mg/L and 2511 mg/L, after 30 h at 30 °C, respectively. The results indicated that survival and GABA production improved upon microencapsulating the bacteria due to the good cell protection provided by soy protein isolate-alginate coating. In long with previous reports, this study proves the potential of microencapsulation toward increased efficiency of GABA production in functional foods.

Milk is an important nutritional food and it fully satisfies the nutritional needs of humans for up to 6 months. Due to the presence of nutrients, milk is a very suitable medium for the growth of a variety of bacteria. Hence some people with the addition of some materials were tried to avoid milk deterioration. The common adulterations in raw milk are the addition of neutralizing agents, water, salt, detergent, hydrogen peroxide, formalin, and others.

In this study, a total of 120 milk samples were collected from milk collection centers of Mazandaran province. Parameters such as acidity, pH, dry matter, density, protein, fat and freezing point were tested. Also, the addition of detergent, formalin, salt, hydrogen peroxide, and sodium bicarbonate were analyzed on the samples.

The results of the experiments showed that there is no significant difference in the amount of acidity, pH, dry matter, density, protein, the fat and freezing point of raw milk between warm and cold seasons (P>0.05). Also, the results revealed that there were some adulterations in cold season in the milk samples including, hydrogen peroxide 1.6%, and detergents 1.6%. But in the warm season, the amount of fraud in raw milk was higher than in the cold season. These frauds included the addition of formalin 1.6%, detergent 3.3%, hydrogen peroxide 3.3% and sodium bicarbonate 11.6%. There was no adulteration of salt and water added in raw milk samples. Overall, there was a significant difference between the incidence of milk adulteration in warm and cold seasons (P<0.05).

The necessity of continual pursuit of importance regarding the inspection and sampling of milk collection and distribution centers and carry out the appropriate tests in order to check frauds aimed to discuss health and complications from taking it to feel.

In this study, the effect of glycerol concentrations (60, 70 and 80 %) and drying temperatures (45, 60 and 75 °C) on physical and mechanical properties of Lepidium perfoliatum seed gum-grass pea (Lathyrus sativus) protein isolate composite film was investigated. Results showed that with increasing the temperature from 45°C to 75°C, the drying time needed for the composite film containing 60 and 70% reduced. Whilst, drying times of the samples with 70 and 80% glycerol were nearly the same. Water solubility, moisture absorption and elongation at break (E%) reduced when the drying temperature increased and the elastic modulus (EM) decreased. With increasing glycerol concentration from 60 to 80 %, water solubility and moisture absorption increased and E% and EM reduced.

Staphylococcus aureus is one of the most important pathogens that cause food poisoning, especially in meat products. Satureja hortensis extract has a excessive effect in preventing the growth of Staphylococcus aureus due to its high content of phenolic compounds in vitro. The aim of this study was to investigate the effect of methanolic extract of Satureja hortensis on the inhibition of growth of Staphylococcus aureus in ground meat and to determine its effective concentration in this product. After extraction of Satureja hortensis extract using methanol, the extract was added to inoculated meat with Staphylococcus aureus at concentrations of 0.06, 0.125, 0.25, 0.5, 1 and 1.5% and after 7, 14, 21 and 28 days total count and growth rate of this bacterium were studied. The results showed that concentrations of 0.25% or more of Satureja sativa extract could inhibit the growth of Staphylococcus aureus and total count in ground meat. On the other hand, sensory evaluation results showed that using up to 0.5% of Satureja sativa extract had a positive effect on sensory characteristics of ground meat. Therefore, the Satureja sativa extract can be used as a natural preservative in meat products, especially ground meat.

Over the recent decades, prevalence of obesity and obesity-related comorbidities such as coronary heart disease, diabetes, hypertension and certain types of cancer has resulted in growing demands for low-calorie foods. However, removal or reduction of high-calorie ingredients from food formulations adversely affect the sensory properties of the final product. The purpose of this study was to develop a low-calorie fiber-enriched breakfast cake with desirable organoleptic properties. To this end, different amounts of apple fiber (0, 10 and 20%) and guar gum (0, 0.5, and 1%) were incorporated into the formulation of a breakfast cake, and the physicochemical, textural, microbial and sensory properties of the resultant samples were investigated in comparison with the control sample. The findings showed that inclusion of apple fiber in formulation of breakfast cake was accompanied by decrease in protein and carbohydrate contents but the fat content was not affected significantly. Conversely, partially substitution of fat with guar gum did not influenced the aforementioned three parameters. While the highest hardness was obtained for the sample with the highest fiber and the least gum (sample T2), the lowest compression resistance was recorded for the sample containing the most gum and the least fiber (sample T2). Moreover, the storage duration had an increasing effect of the hardness of all the samples. Apple fiber and guar gum both decreased the calorie of the product but the former had a more pronounced effect, so that the sample with the highest fiber and gum (sample T6) showed the lowest calorie. The results of sensory evaluation revealed that the samples did not significantly differ in terms of taste, odor and color scores; however, the samples incorporated with higher apple fiber and lower guar gum had lower texture and total acceptance scores

Food fortification for the prevention and treatment of micronutrient deficiency disorders is one of the most widely methods that because of its low cost and no need to change dietary habits was used all around the world. In grain fortification, immersion and spraying methods are usually used, and extrusion processes are used for cereal flour. In extrusion fortification method, the used extensions are into the food matrix so their output during different processing stages is less than other enrichment methods. In this study, rice reconstituted by extrusion cooking method, was enriched with vitamin D3 (100000 IU/g) at concentrations of 2.5, 5, 7.5 and 10 µg/100g and Zinc (as Zinc oxide) concentrations of 5, 10, 15 and 20 mg/100g and then remaining of Vitamin D3 and Zinc were measured before and after extrusion and before and after cooking in 30 days intervals during 180 days storage period. According to the results, storage period and also the cooking process at all initial concentrations have a significant effect (p<0.5) on Vitamin D3 and Zinc stability of extruded fortified rice so that the average content of Vitamin D3 and Zinc at the end of storage period of 180 days showed a loss of 32.9 and 11.3% (before cooking), 38.9 and 24.0% (after cooking), respectively but these materials had a good stability during the extrusion process. Overall, during the different processing stages, the average content of Vitamin D3 and Zinc showed a loss of 87.1 and 39.0%, respectively.

Hawk (Crataegus spp.) is a rich source of natural antioxidants, especially flavonoids and anthocyanin, and these bioactive compounds have various beneficial effects on health and well-being. In this study, hawthorn fruits extract were prepared by ultrasound (for 30 minutes), microwave (150 s) and ultrasound- microwave (30 min ultrasound + 150 s microwave).  After applying these pre-treatments, the extract of fruits was extracted with Ethanol. A sample without any pre-treatment was selected as a control. The total phenolic content, scavenging activity of DPPH, reducing power assay, acidity and determination of important compounds (by gas chromatography / mass spectrometry) of the samples were investigated. Statistical analysis and optimization process were performed through completely randomized design using SAS software. The results showed that the use of different pre-treatments resulted in increasing the total phenolic content, scavenging activity of DPPH and reducing power in extracts. The total phenol content of sample obtained from the pretreatment of ultrasound-microwave was higher (9.89%) than control sample. The analysis of the acidity data showed that the pre-treatment type did not have significant effect on the acidity of the samples (p <0.01). The using microwave- ultrasound pre-treatment resulted in increase in the extract compounds and the bis-2-ethylhexyl-phethalate was the most detectable combination in the extracts. It was concluded, that combination of microwave and ultrasound was the best pretreatment for extraction of hawthorn fruit.

The use of nanotechnology can create new important applications in various fields of agricultural resulting in improvement of plant yield, and increase of growth and photosynthesis. Using this technology, the techniques in product management can be improved. The absorption rate of nanoparticles varies in plants, depending on type of plant, chemical composition and size of particles. Nanoparticles of titanium dioxide (TiO2) are widely used in agricultural industries to enhance plant growth properties. In this case, the aim of this study was to study the effect of titanium dioxide nanoparticles on photosynthetic pigments and some biochemical properties and antioxidant enzymes of Rosmarinus officinalis L. This research was carried out in a completely randomized design with three replications in the greenhouse of Sana Institute of Higher Education of Sari in 2017-2018. Different concentrations of titanium nanoparticles in seven levels (0, 20, 40, 80, 100, 200 and 400 ppm) were sprayed onto rosemary plants in three stages with a one-hour interval. The results showed that the contents of chlorophyll a, chlorophyll b, total chlorophyll and carotenoids were increased by increasing the concentration of titanium nanoparticles, since the highest total chlorophyll content and carotenoids were related to 200 ppm nanoparticle titanium concentration. Also, the effect of titanium nanoparticle on the amount of soluble sugar indicated that by increasing the concentration of titanium nanoparticles as 100, 200 and 400 ppm nanoparticles, there was an increasing trend in this trait. In addition, there was a significant increase in the amount of antioxidant enzymes studied in high concentration of titanium nanoparticles (80, 100, 200 and 400 ppm).

It is important to control the ripening stages of agricultural products during storage and their quality grading based on their ripening stage. Edible coatings can prolong the storage life of agricultural products and protect them through the handling, storage, processing and marketing. The purpose of the current study was to develop an artificial vision system for quality control and segregation of cherry tomatoes in two different conditions including with and without Aloe vera gel coating. For this purpose, physicochemical properties including titrable acidity, total soluble solids and firmness of cherry tomatoes were measured in both conditions. Based on these properties, the ripening index (RPI) was determined and the samples were classified to MS1 and MS2 according to the ripening stage. Subsequently, the samples were classified using color features, color texture features separately and their combination through principal component analysis (PCA) and back propagation neural network (BPNN). Classification results showed that the use of color and color texture features combination made the classification more accurate; PCA and BPNN methods were able to segregate the samples with high accuracy (85.72 and 98.21, respectively) using the 21 color and color texture features. The higher accuracy of the BPNN method is due to its nonlinear performance. The results of this study indicate that Aloe vera gel is promising in delaying the ripening process of cherry tomatoes and artificial vision system can be used as a non-destructive method for evaluation of cherry tomato ripening level based on the color and color texture features.

Milk is considered to be a perfect food for humans, but combined cheating and lack of it’s fast  recognition by existing machines, causes the dairy industry has been challenged. In this regard,  alternating current electrical conductivity is used to detect some of the properties of milk, but direct current is not permitted due to disadvantages such as electrode polarization and milk hydrolysis. In this research, a device have be made that work based on the electrical resistance of the milk. In this device, by passing a direct current through the milk, The suspended ionic material is absorbed at the electrode surface and causes the dilution of the milk. The value of the electrode current density at resistances of 1.48, 1.85, 2.5, 3.7 and 7.4 k𝛀 with two aluminum and steel electrodes at temperatures of 17.2, 20.7 and 23.7 degrees Celsius, at intervals The electrodes 1, 2 and 3 cm were measured in volumes of 15, 30 and 45 cc for two minutes, and the effective factors in designing the device to reduce the effects of double  disadvantages were optimized geometrically. The results show that the device with the steel electrode and cell coefficient of 0.33 cm-1 can optimally measure the quality of 45 cc milk at 25 oc. the current density of the steel electrode is lower than that of the aluminum electrode and increases with decreasing circuit control resistance, and for reducing the polarization of the electrodes and the rate of hydrolysis, with a resistance of 3.2 k𝛀, and a constant cell coefficient with a minimum oscillation, it’s optimum value was obtained 1.5 mA And the temperature, volume of the milk and the distance of the electrodes did not affect it’s value.

The present study aims to investigate replacing sugar with 0, 25, 50, 75 and 100% grape syrup in producing sponge cake and its effect on properties such as texture stiffness, volume, density, and porosity during 7 days after production. The results demonstrated that increasing grape syrup percentage in the cake samples changed their properties significantly. The samples containing 50 and 100% grape syrup had the most density, and  least stiffness, and  most volume, respectively. Comparing the mean of treatment effect in day suggested that the most stiffening occurred in seventh day. In addition, the sample containing 25% grape syrup has the most porosity. The mean and standard deviation of treatment effect of the blank indicated that the stiffening of all the samples  increased compared with the blank. Thus, there is a significant statistical difference between the samples in this regard. Therefore, the sponge cake prepared by 100% replacement of sugar with natural sweetener (grape syrup) could be introduced as the best sample because of having   the most volume,  least stiffening amount, more desirable taste and appearance.

Reducing sugar consumption in food product formulation is one of the strategies for achieving healthy food, but this has a detrimental effect on the texture and sensory properties of the product. The taste perception has been proven to be influenced by the structure and composition of the foods. In this study, the aroma and texture related cross-modal interactions on perceived sweetness of flavored milk and sugar reduction were investigated. Different treatments of chocolate milk included control treatments with different sugar content (4.5, 6 and 7.5%) and 14 treatments containing different amounts of stabilizer (0.10, 0.15 and 0.20%), vanilla (0, 0.002 and 0.004%) and caramel (0, 0.025 and 0.05%). pH, dry matter and viscosity of all treatments were determined and then all treatments were evaluated by a trained evaluator group and a semi-trained evaluator group. Data analysis was performed using TOPSIS and AHP methods to select the best product. Results showed that with decreasing sugar, pH decreased and with increasing stabilizer dry matter and viscosity increased (P<0.05). Samples containing the combination of vanilla and caramel flavors with reduced sugar by 20% had the best sensory properties. Treatments containing stabilizers also increased the perception of sweetness in the presence of flavors. Overall, this study showed that the sugar content in cocoa milk formulation can be successfully reduced until 20% by the simultaneous use of stabilizers and vanilla and caramel flavors.