نشریه ارمغان دانش
سال بیست و پنجم شماره 4 (پیاپی 141، مهر و آبان 1399)

Cell-therapy is an important science because of using to treatment of critical-sized bone defects. Recent studies in this field suggest that human endometrial derived stem cells can be a great source. On the other hand, graphene and its derivatives, mainly graphene quantum dots (GQDs) have recently attracted much attention as effective factors in differentiating stem cells towards osteoblastic lineage. So the aim of this study is evaluation of the effect of adding GQDs to standard osteogenic medium and the effect it has on the differentiation of endometrial stem cells.

The present research was an experimental study carried out in the cell culture research laboratory of Shahid Chamran University of Ahvaz in 2019.  After the cell isolation by collagenase enzyme and purifying endometrial stem cells extracted from uterus tissue, the cells in two groups including the control group that the cells was cultured in standard osteogenic medium while the cells of the experimental group were cultured in the same medium with the addition of graphene quantum dots at a concentration of 50 μg mL-1.Then the data was evaluated using alizarin red staining, the activity of alkaline phosphatase, and qRT-PCR method for gene expression.  Data were analyzed using one-way ANOVA.

In here for the first time, the results of the interaction between GQDs and endometrial derived stem cells were showed in in vitro condition and confirmed that the presence of GQDs in the osteogenic medium can lead to significant incrassation in osteogenic differentiation efficiency of endometrial cells. Indeed, alkaline phosphatase activity and formation of calcium nodules (that are given more intensity by alizarin red) were significantly increased more in the experimental group compared with the control group which indicates earlier and better activation of osteogenesis in the experimental group. These results were confirmed with the significant up-regulation of phenotypically related osteogenic genes (Runx2, osteopontin, and osteocalcin) which were measured using a qRT-PCR technique.

 These results show that using a standard osteogenic medium with addition of graphene quantum dots in conjunction with endometrium derived stem cell can have potential use in the field of osteoblast differentiation with a significant efficiency increase and cell-therapy.

Recently, the drugs with marine resources have been considered due to the lower costs and greater compatibility with patients. In the present study, the extracts of three marine organisms from the Persian Gulf; Holothuris parva, Ophiocoma scolopendrina and Echinometra matheai, were prepared and evaluated their in vitro anti-leishmaniasis effects.

All three marine organisms were collected from the coastal waters of Bushehr province and after separating their shell from the viscera, were extracted by percolation method using different solvents. All prepared extracts were evaluated for anti-leishmanial activity on Leishmania major promastigotes in vitro.

Statistical analysis showed that the extract type and the concentrations used (dose) had a very significant effect. Also, the dual interactions of extract and dose, extract and solvent, as well as the triple interaction of extract, solvent and dose were significant on the inhibitory rate. The highest anti-leishmanial activity was related to methanolic extracts of Holothuria Parvachr('39')s shell (88.65%) and Echinometra matheaichr('39')s viscera (87.48%) at 800μg/ml concentrations, and the lowest activity was obtained in aqueous extract of Ophiocoma scolopendrina (43.01%) at 20μg/ml concentration.

Considering the anti-leishmanial effects of all three marine organisms studied in this research, and the presence of their rich reserves in the Persian Gulf in another way, by performing a series of complementary studies in vivo and identifying the effective compounds of these extracts, efficient steps can be taken to produce effective drugs for the control and treatment of cutaneous leishmaniasis.

During school, students experience many emotional and emotional changes at the same time as physical and cognitive changes, including anxiety and anger, which can affect interpersonal relationships and be destructive if they are out of control. Because for children and adolescents, emotion regulation training is a key point forgotten in parenting education. The aim of this study was to investigate the effect of emotion regulation therapy training based on dialectical behavioral therapy on reduce  anxiety and anger students.

The present study was a quasi-experimental with a pretest-posttest design with a control group. The statistical population of the present study was all female high school students in Lande, Kohgiluyeh and Boyer-Ahmad Provinces in the academic year 2017-2018. Among them, 36 people were selected according to the conditions of entering the research by the available sampling method and were randomly selected in two experimental groups (18) and control (18). The Beck Anxiety Questionnaire (1990) and the Novako Anger Scale (1986) were used to gather information in two stages, pre-test and post-test. Dialectical Emotion-Based Emotional Behavioral Training Therapy session was performed for nine 90-minute sessions per week for the experimental group, but the control group did not receive this training. Data were analyzed using multivariate analysis of covariance and SPSS-24 statistical software.

The results of data analysis showed that emotion regulation therapy based on dialectical behavioral therapy was effective in reducing anxiety (F=14.077; P <0.001) and anger (F=7.422; P <0.004); This means that in the post-test, the mean score of anxiety (M=23.10; SD=4.57) and anger (M=38.06; SD=1.33) decreased significantly in the experimental group compared to the control group.

Based on the findings of the present study, it can be said that teaching emotion regulation based on dialectical behavioral therapy leads to reduction of students chr('39')anxiety and anger, and this treatment method can be used to prevent and reduce studentschr('39') anxiety and anger.

Understanding the risk factors of laparoscopic cholecystectomy for open surgery is essential. The purpose of this study was to evaluate the preoperative laboratory findings in predicting the severity of laparoscopic cholecystectomy.

This cross-sectional study was performed on 122 patients undergoing laparoscopic cholecystectomy. Before surgery, patient information such as age, sex, laboratory findings such as WBC, ALT, AST, T Bill were recorded in the checklist. Finally, the degree of difficulty was divided into two degrees of easy (below 60 minutes without complications) (over 60 minutes with complications such as bleeding or open surgery). Finally, SPSS software was used for data analysis. The level of significance was considered to be less than 0.05.

28 (22.4%) males and 97 (77.6%) females were studied. Mean age of patients was 44.66 13 13.85 years. The number of difficult operations was 35 (28%) and 90 (72%) easy operations. Transplant surgery was done in 3 cases. There was a significant relationship between preoperative laboratory findings between AST (P value: 034), TBC (P value: 008), WBC (P value: 000), and difficulty of operation, but no significant relationship between ALT (P value: 0). 065), no significant relationship was found with the severity of cholecystectomy.

Preoperative laboratory findings are capable of providing valuable data in predicting the severity of laparoscopic cholecystectomy.

Urinary tract infections are one of the most commonly reported nosocomial infections caused by colonization of E. coli in the mucosal epithelium and in the formation of microbial biofilms, which damage the host tissue. The aim of this study was to determine the amount of biofilm formation of uropathogenic E. coli based on urinary tract stones of people with urinary tract infection.

In this study, 380 patients with positive urine culture with Escherichia coli bacteria were studied. Of these, 34 patients had urinary stone and urinary tract infection with E. coli at the same time. These rocks were sent to the laboratory in accordance with sterile conditions. To maintain and maintain a long-term bacteria to collect and perform other tests in such a way that the bacteria themselves are not harmed, they are stored in a glycerol stock state. To determine the biofilm formation capacity, this bacterium was investigated by Microtiter Plate method.

The results showed that a total of 34 stone samples were examined using conventional methods containing uropathogenic E. coli. These microorganisms showed a low biofilm strength of 18%, very low power, 20% moderate power and 59% high power.

In this study, the presence of infection with urinary stones as a suitable bed and surface for the uropathogenic E. coli and, as a result of biofilm formation of this bacterium on the surface of the stone, causes more urinary tract infection and also resistance an antibiotic in the strains that causes the urinary tract infection.

Infertility is one of the most important medical and social issues in all societies. Access to accurate and up-to-date statistics on infertility and related causes can be helpful in formulating policies to assist infertile couples and referring them to infertility and treatment centers and solving their problems. The purpose of this study was to evaluate the epidemiological status of infertility in Boyer Ahmad city in 2018.

The present study is a descriptive-analytical study which was performed on 1536 couples who referred to all health centers in Boyer Ahmad city affiliated to Yasuj University of Medical Sciences. The data were collected by interview using a researcher-made questionnaire. The validity of the questionnaire was confirmed by content validity.

The mean (SD) age of the infertile women was 33.01 6. 6.38 and 38.45 8. 8.39 years in the present study. The prevalence of infertility in Boyer Ahmad was 8.13% with 44.06% primary infertility and 55.93% secondary infertility. The mean (SD) BMI of women was 27.93 5. 5.49, indicating that the majority of women were overweight. In primary infertility, 42.3% were female, 26.9% were mixed, 23.1% were male and 7.7% were idiopathic.

The prevalence of infertility in Boyer Ahmad city was lower than the national rate and previous studies. In the frequency distribution of infertility type, secondary infertility was more frequent. Regarding sampling based on SIB system and differences in infertility statistics in the province compared to Iran and the world, this finding may be due to the low number of infertile couples surveyed due to lack of information on infertile women in SIB system. . Therefore, further steps are recommended to lower the deficiencies of the SIB system in order to obtain accurate population statistics of infertile women. Providing more health facilities in government centers also seems to help.

Many studies have investigated the influence of exercise on diabetes-related factors. The main purpose of the present study was to examine the effect of endurance training with different intensities on hba1c in patients with diabetes mellitus, a systematic review, and meta-analysis of articles in Iran.

For the present study, databases from ScienceDirect, PubMed, Scopus, the science of the Web, SID, Magiran, and Google Scholar from years 1381 to 1398 were searched with keywords After initial screening, the full text of the articles was evaluated and the articles that met the inclusion criteria were analyzed. 21articles were reviewed, of which 11coincided the criteria for systematic review and meta-analysis.  381 diabetic patients divided into two groups of 169 and 237, respectively, in the experimental group (99 females, 134 males) and the control group (57 females, 81 males). The mean age of the subjects in this study was 52.62 years. Intervention effects were evaluated as mean difference (MD) with a random effects model.

The results of the meta-analysis showed that both moderate and moderate aerobic exercise decreased HBAIC percentage. Endurance training in general reduced HBAIC levels significantly.  In the subgroup analysis, endurance training was moderate (P = 0.001, SE = 0.27, V = 0.07, CI = -1.51 -0.44 Z = -3.58) and high intensity (P = 0.001, SE = 0.22, V = 0.05, CI = -1.33 -0.46 Z = -4.05)

Different intensities of endurance training had a significant effect on HBA1C levels in type 2 diabetic patients. However, further studies of higher quality are still needed to investigate the differences between intensities.

 Food insecurity is recognized as a serious public health problem in the world and has attracted the attention of the public, professionals, and policymakers. Food insecurity and hunger can affect social and mental health as well as physical health. Given that women makeup, half of human society and have a significant and far-reaching impact on public health; this study aimed to assess the food security status among employees’ women in the Department of Education.

 In this descriptive-analytical study, among female teachers of Yasuj Education Department, 400 female teachers were randomly selected using a list of random numbers assigned to teachers with a simple randomization method. The Radimer-Cornell questionnaire was used to assess the food security, and a Seca scale and a tape measure were used to measure weight and height respectively. After collecting the data, statistical analysis was performed using SPSS software version 19.

 In the present study, the mean and standard deviation of participant’s age was 37/87±6/89 years and the mean and standard deviation of body mass index was 25/90±3/70 kg/m2. The results of this study showed that in terms of body mass index, 123 people had a BMI below 25 (30/8 % = normal weight), 197 people had a BMI of 25 to 30 (49.2% = overweight) and 80 people had a BMI of more than 30 (20 % = obese). 70/5 % of teachers suffered from varying degrees of food insecurity, and only 29/5 % of the women studied had complete food security. They reported food insecurity on household (50%), individual (12%), and child (8.5%) scales. In examining the factors related to food security, the results of statistical tests showed that economic status, housing status, spousechr('39')s job, and participantschr('39') education have a significant statistical relationship with three scales of food insecurity (household, individual and child).

 The results of the present study showed that there are degrees of food insecurity in a large part of the female teacherschr('39') community and that social and economic factors also play an important role in this food insecurity. It seems that it is possible to take steps to improve this problem with appropriate policies and executive strategies.

Thalassemia syndromes are the most common inherited hemoglobinopathies in the world characterized by various degrees of defective production of the alpha or beta globin chains. Impairment in alpha chain or beta chain synthesis leads to alpha thalassemia and beta thalassemia syndromes respectively.
Iran is one of the countries located on the thalassemia belt therefore this disease is significant in our country. The most important component of treatment in beta thalassemia is packed red blood cell transfusion. Thalassemic patients need lifelong transfusions and the consequence of these repeated transfusions is iron accumulation in different organs, such as heart, liver and endocrine gland. Iron overload is one of the major causes of morbidity in patients with thalassemia major necessitating iron chelation drugs to improve survival of patients. Three iron chelating agents are currently commercially available: deferoxamine (DFO), deferiprone (DFP), and deferasirox (DFX). DFO is the first chelating agent that became available. DFP is the first oral iron chelator and DFX is the most recently approved chelator for transfusional hemosiderosis. The purpose of this article is to summarize the main pharmacokinetic characteristics and side effects of iron chelator drugs in the field of thalassemia.

In recent years, computers and the Internet have entered all parts of people’s life. One of the problems that may arise is Internet addiction, which has been reported as a new disorder with a number of related psychiatric disorders. This study aimed to determine the structural relationships between mental health and internet addiction in adolescents, regarding meditating the role of attachment styles.

This study was descriptive-correlational. Participants were 310 high school students of Karaj in 2018-19, selected by purposive sampling. The instruments were the Goldberg and Hiller Health Questionnaires (1979), the internet Addiction Questionnaire (Yang, 1998), and Attachment Styles (Hazan and Shaver, 1987). To measure the validity of the structure of the research variables, the measurement model and Amos software were used.

The results of the models showed that all research scales have a suitable fit. Mental health, in addition to its direct role, has been able to significantly explain the symptoms of Internet addiction through attachment styles.

Based on these findings, it can be concluded that paying attention to a conscious parenting can lead to a secure attachment style. And consequently, by creating mental health, the harm of internet addiction in adolescence can be prevented. It is suggested to consider the role of mental health and attachment styles in prevention and treatment interventions of internet addiction.

Acute rheumatic fever is an autoimmune disease that mainly affects the large joints. Early treatment of a throat infection following group A streptococcus prevents of acute rheumatic fever. Given the low prevalence of the disease, especially in a situation where there is excessive use of antibiotics in Iran, this study intends to report a patient with Acute rheumatic fever with mild criteria.

the patient was 14-year-old man that came to internal medicine clinic due to weakness and joint pain. The patient had pain in the joints of the wrists and ankles, as well as the knees, and was seen as an immigrant in the patient. He had a history of fever and sore throat a month before the visit. The patient did not describe the skin lesion. On examination, the patient had vital signs of BP: 100/60 mmhg, PR :90 / min, RR:25, and T:37.7. The throat examination was slightly erythematous, and without exudates, the cardiac examination was tachycardia without murmur. On examination of the joints, only the left knee joint had redness, and it was warm to the touch. In the laboratory tests, ASO: 400, ESR: 94 and CRP: 76 were. The patientchr('39')s chest x ray, ECG, and echocardiogram were normal. Based on the symptoms and laboratory tests in Jones criteria, the diagnosis of ARF was confirmed. Then, the initial treatment, naproxen and co-amoxiclav was started, and explained for the patient to receive injection of penicillin benzathine once a month until the age of 21.

Acute rheumatic fever should be considered as part of the differential diagnosis of polyarthritis in the young patients, so that, if present, with appropriate treatment and prophylaxis, we can to able reduce the social costs from rheumatic heart disease.