نشریه بیولوژی کاربردی
سال دهم شماره 3 (پیاپی 39، پاییز 1399)

Study of antimicrobial effects of aqueous and methanolic extracts of vegetative and reproductive structures of A. Chamaecistus and A. austro-iranica on several fungi and bacteria

Aquatic and methanolic extracts prepared from Ajuga Chamaecistus, A. austro-iranica were tested for antibacterial activity against gram positive (Satphyloccocus aureous and Streptococus pyogenes) and gram negative (Pseudomonas aeroginosa and Proteus vulgaris) bacteria and Aspergillus niger and fusarium solani. The vegetative and generative shoots of plants were powdered, sterilized, and extracted at 4°C with methanol and water. The concentrations of 50, 100 and 200 mgml-1 of methanolic and aquatic extracts were used to detect the minimum inhibitory concentration (MIC). All experiments were tested three times. The antibacterial effects were evaluated using the cup-plate and antifugal activity using cup-plate and pour plate

The antibacterial effect of aqueous extracts showed that only the leaf extract (vegetative extract) of A. chamaecistus is effective on S. aureus showing that in gram-negative bacteria, the presence of a lipopolysaccharide wall causes greater resistance. Methanolic extracts of both species were more active than aqueous extracts and had antimicrobial effects on all tested bacteria as well as A. niger. In both species, the extracts of the generative parts showed a stronger antifungal effect. In different species of this genus, the presence of phenolic compounds, tannins and terpenoids that have antimicrobial properties confirms the antimicrobial properties of this species. However, the amount of their effect depends on plant and microbe species, the tested parts (plant organs), the extracts concentration, the sampling season, the sample age and soil type.

In this research Arum species investigated based on molecular and morphological traits in order to new classification from Iran.

Plant materials were collected from nature or were prepared from herbarium. Statistical analysis was performed on morphological characters of Arum L. species. 29 qualitative and quantitative morphological characters were evaluated. trnL-F as plastid marker is widely used to infer phylogenetic relationships. Phylogenetic analyses were conducted by the Bayesian inference and maximum Parsimony methods.

Cluster analyses by ward method were classified the studied species based on morphological traits in three clusters. ClusterI included A. giganteum. ClusterII had A. conophaloides and A. viresence. ClusterIII divided in two groups: A. maculatum, A. kotschyi and A. korolkowii. Dendrogram of relationships was constructed by UPGMA, demonstrated four main clusters. A. maculatum and A. giganteum placed in separated clades. ClusterIII include two subclades: SubcladeI: A. conophaloides and subcladeII: A. virescence, A. korolkowii and A. kotschyi. Cladistics analysis of phylogenetic relationships indicated that all species constituted monophyletic group within Arae clade. A. maculatum was separated in different place. A. kotschyi and A. korolkowii with A. rupicola from gene bank were introduced as sister groups. Arum virescens, A. conophaloides and A. giganteum were separated from A. ruoicola. In general, the morphological and molecular results are consistent.

Staphylococcus aureus is an important human pathogenic bacterium and one of the pathogens that commonly causes biofilm infections in the clinic. Antibiotic resistance has spread worldwide in Staphylococcus aureus and is a serious problem for the treatment of patients. In this study, the effect of thyme essential oil of Shirazi and cinnamon on the formation of biofilm in Staphylococcus aureus strains has been investigated.

In this study, 20 clinical samples of Staphylococcus aureus were isolated from the urine of patients admitted to the intensive care unit of Taleghani Hospital in Tehran. The microdilution test was performed to determine the minimum inhibitory concentration (MIC) of Zataria multiflora and cinnamon essential oils on strains. The ability of biofilm formation in the collected strains was assessed by microtiterplate and the effects of Zataria multiflora and cinnamon essential oils were evaluated on biofilm formation.

In this study, 75% of Staphylococcus aureus strains formed strong biofilm and 25% moderate biofilm. Treatment of Staphylococcus aureus strains with Zataria multiflora essential oils in MIC concentration revealed that biofilm formation were 40% weak and 60% strains did not form biofilm. Treatment of strains with cinnamon essential oil at MIC concentration was observed to be 25% strong biofilm and 75% moderate biofilm. The present study shows the effect of essential oils of Zataria multiflora and cinnamon in reducing the biofilm formation of Staphylococcus aureus strains.

Social linguistics is a type of linguistic study that is known as a cultural subject that is closely related to the social relations of the community that speaks that language. The purpose of this study is to identify and record the different names used for trees, shrubs and shrubs in different regions of Gilan, Mazandaran and Golestan provinces from Astara to Ramyan. These provinces also had local names registered.

The present research has been done by field method and library study. Although all the researchers in this study are fully acquainted with the Gilaki language and dialect, however, to determine the exact Persian equivalent of words and tree-related entries in different local dialects, interview speakers of this language (especially people over 60 years of age Year) did. All the words and terms of this research are related to the most important words and terms related to the trees, shrubs and bushes of the families of the tribes of Aceraceae, Hamamelidaceae, Leguminosae, Fabales, Betulaceae, Ebenaceae, Fagaceae, Buxaceae, Juglandaceae in different regions of Gilan, Mazandaran and Golestan.

In this study, 102 local names for the trees of the genus Aceraceae (23 local names), Hamamelidaceae (2 local names), Leguminosae (14 local names), Fabales(18 local names), Betulaceae (9 local names), Fagaceae (20 Local names), Buxaceae (6 local names) and Juglandaceae (10 local names) in Iran, which are mainly the names used in the three provinces of Gilan, Mazandaran and Golestan in different dialects, were recorded. Based on the results of this research, Acer laetum with local names of Bezberg (in Gorgan), Mirzaberg (in Ramyan), Pellet (in Gilan), Milky (in Noor, Kojoor and Mazandaran) and Zbandar (in Bandar-e-Gaz); Acer opulifolium with the local names of Cerco (in Ramyan) and Sefid Cerco (in Katul); Acer platanoides with the local letters Kirk (in pilgrimage) and Kakum (in its length); Alnus glotinosa with the local names of Siatuse (in Lahijan), Tosa (in Rudsar), Tose (in Rasht), Alder (in Gorgan and Mazandaran) and Yellow (in Astara and Tavalesh) are called.

The aim of the study was to investigate the management of simultaneous catch season and spawning period of giant cuttlefish and determine a new solution for optimal management for conservation and continuity of fisheries in the region.

Samples were collected from the catches of traditional gear of traps at landing places of Bushehr and Khuzestan. 100 to 150 samples were randomly selected and sorted by sex. Reproductive status in males and females were studied using gonadosomatic index (GSI) and statistical calculations.

The result of GSI in males and females indicated the highest reproductive activity in February and March, and then reproductive activities were reduced in both sexes. Sex ratio was ½ and Chi-squared (X2) tests gave for both sexes, indicating that the ratio of males to females was significantly different (p> 0.5). The results of present study show that opening period of the giant cuttlefish in the studied area is simultaneous with maximum spawning period of the species. Based on the results of the present study, it is necessary to develop a new management for exploitation of the giant cuttlefish in the area.

The aim of this study was comparing the anatomical characters of leaves and petioles of 3 species of pear (Pyrus boissieriana Buhse, Pyrus communis L, Pyrus hyrcana Fedor) in Guilan Province.

In this study, for anatomical study of species, at first, plant samples were collected. Then the manual section was performed in the laboratory. Carmine staining was used in order to stain the samples. After staining, slides were prepared from the cross-sectional area. For observing the transverse sections of leaves and petiole, optical microscope equipped with a scaled lens was used. Then, the data obtained from anatomical characters were statistically analyzed.

Leaf and petiole thickness were different among three tested species. The highest midrib thickness belonged to p < /em>. communis (682±1.11μm). The lowest midrib thickness was observed in p < /em>. hyrcana (431.66±1.05μm). Anatomical characters are effective and important in species segregation. Therefore, anatomical characteristics in different plant species will be useful in terms of taxonomic classification.