مجله سلول و بافت
سال دوازدهم شماره 1 (بهار 1400)

The aim of the study to evaluate the impact of aerobic training on cardiometabolic and inflammatory changes evoked by myocardial infarction in rats.

In this experimental study, two-month-old male Wistar rats were infarcted and randomly divided into two groups with n = 11 in each group: sedentary +MI (MI+S) and aerobic training +MI (MI+EX). After the acclimatization period, ISO (85mg/kg) was subcutaneously injected into the rats at 24 h intervals for 2 days. ISO-induced myocardial damage was indicated by increased serum cardiac-specific troponin I levels and ST-segment elevation. The MI+EX rats were conditioned to run on a treadmill for 10 weeks. 48 hours after the last training session, a venous sample was collected to determine metabolic parameters, including carbohydrate antigen 125 and cystatin C. The independent t-test was applied to analyze the data (P<0.05).

As predicted, the MI+S group had an increase in carbohydrate antigen 125 (P=0.03), cystatin c (P=0. 01), and troponin-I (P=0.002) concentrations. Unlike the MI+S group, in the MI+EX group carbohydrate antigen 125 (P=0.01), cystatin C (P=0.04), and maximal treadmill exercise test (P=0.01) were improved.

Our findings suggest that early aerobic training may be useful in preventing the negative changes in functional, inflammatory, and metabolic parameters related to MI.

This study was conducted to determine the effect of cold plasma on growth and anatomical features of Foeniculum vulgareL. plants.

After preparation, fennel seeds were exposed to cold plasma irradiation for 0, 5, 10, and 20 minutes. After germination and initial growth of seedlings, the anatomical features of roots stems and leaves grown from seeds treated with cold plasma were investigated using Carmen Zaji staining.

The results showed that cold plasma was able to make changes in the pattern of differentiation of xylems of the fennel plant, which was mainly dependent on cold plasma time. These changes include an increase in the size of xylem cells and a change in the distribution pattern of the vascular system in the leaves, stems, and roots.

It was concluded that cold plasma treatment causes changes in the anatomical features of leaves, stems , and roots, which can be responsible for the beneficial effects of cold plasma on seed germination characteristics and early plant growth.

This study aimed to investigate the apoptotic effects of menthol on CT-26 colon cancer cells.

In this experimental study, CT-26 colon cancer cells were treated with 1 to 5 mM menthol for 24 and 48 h. The viability of colon cancer cells was calculated by MTT assay. DAPI staining was used to evaluate apoptosis induction and the expression of Bax and Bcl2 genes was evaluated by Real-time-PCR technique.

The results from the MTT assay showed that menthol inhibits CT26 cell growth in a dose and time-dependent manner. The results from DAPI staining indicated that menthol leads to condensation and breakdown in chromatin. The results of real-time PCR showed that expression of Bax increased at 3 mM concentration of menthol and Bcl2 gene expression increased in cells treated with 2 mM concentration of menthol compared to control.

The results showed that menthol induces apoptosis in CT-26 colon cancer cells. Therefore, the use of this monoterpene can be considered as a promising strategy in clinical studies of colon cancer.

In this study, the effect of Artemisia herba alba essential oil on the survival rate and expression of ODC1 and ADA genes in human breast cancer cell line MCF-7 was studied.

The essential oil (EO) of A. herba alba was extracted by Clevenger. Essential oil composition is analyzed by GC-Mass. MCF-7 cell line was treated by increasing concentrations of EO from 0.01 to 0.03 µg/ml. Then cytotoxicity of EO was investigated by MTT assay and the expression of ODC1 and ADA genes were measured by the Real-time PCR method.

Most essential oils included Camphor, Chrysanthenyl Acetate,  and Davanone. Results showed that by increasing the EO concentration, the cytotoxicity was significantly increased and the calculated IC50 for 24, 48, and 72 h were 0.02104, 0.01858, 0.011751 µg/ml. Moreover, at the concentration of 0.02 µg/ml of EO the expression of the ODC1 and ADA gene decreased by 2 and 1.2-fold against the control, respectively.

The cytotoxicity of A. herba alba EO, which was observed in this study, seems to be the result of decreased expression of ODC1 and ADA genes in the MCF-7 breast cancer cell line.

This experiment was carried out to evaluate the effect of different sources of selenium in daily feed intake (intrinsic effect) on roosters’ semen under oxidative stress in 28 week-old Ross broiler breeder roosters.

A total of 24 birds were divided into 4 experimental groups and 6 birds per replicate in a completely randomized design. Experimental groups included: 1) control group (Con) without any treatment, 2) as control group but birds were administrated with Dexamethasone (Dex), 3) as group 2 + birds consumed selenomethionine as organic selenium (0.3 mg Se/kg of feed intake) (OSe+Dex) and 4) similar to group 3 but birds consumed Sodium Selenite as the inorganic source instead of an organic one. Oxidative stress stimulation was induced by injecting dexamethasone (0.4 mg/kg BW) 3 times every other day for 1 week.

Consumption of selenium sources, especially organic form, resulted in higher total and progressive sperm motility, sperm viability, and membrane integrity (99.56, 59.06, 87.75, and 88.97%, respectively), versus Con (79.4, 42.53, 78.33 and 80.78%, respectively) and Dex (62.2, 27.03, 73.57 and 71.92%, respectively) (P<0.05). Also, the profile of biochemical parameters such as Total Antioxidant Capacity (TAC), Glutathione Peroxidase (GPX), and Super Oxide Dismutase (SOD) in the groups OSe+Dex (146.5, 85.91 and 1.55 U/ml, respectively) and ISe+Dex (122.54, 63.28 and 1.37 U/ml, respectively) were better than Con (121.78, 65.27 and 1.26 U/ml, respectively) and Dex groups (108.62, 52.51 and 1.05 U/ml, respectively) (P<0.05).

Overall, it seems an organic form of selenium resulted in better semen properties than inorganic form.

The aim of this study was to investigate the morphological, physiological, and pollen grain and ovule developmental changes under Cd stress in Artemisia annua L.

In this study, Cd accumulation in plant tissues, growth factors, relative water content of tissue, membrane stability index, photosynthetic pigments content, soluble carbohydrates content, total protein content, antioxidant enzymes activity, seed protein pattern and, developmental stages of pollen grains and ovules were studied under foliar treatment of Cd (0, 75, 150 and 225 mg l-1).

The results showed that A.annua can accumulate Cd. Significant decrease in growth factors, relative water content, membrane stability index, photosynthetic pigments content, soluble carbohydrates content and increase in total protein content, antioxidant enzymes activity were observed. Changes in seed protein pattern, embryonic sac cells degeneration, and the increase of abnormalities in microspores and pollen grains were the abnormalities observed in the plants under treatment.

According to the results of this study, A.annua has a high resistance to Cd stress and can survive under high concentrations of Cd. The results indicate that Cd can affect the developmental stages of reproductive organs and causes a variety of abnormalities during the developmental stages of male and female reproductive organs but it seems that A.annua has been able to resist the negative effects of Cd by changing its physiological properties and proteins contents.