مجله دانشگاه علوم پزشکی جیرفت
سال هشتم شماره 2 (تابستان 1400)

Unilateral ureteral obstruction (UUO) is one of the causes of kidney injury. Oleuropein is known as one of the strongest natural antioxidants. The aim of this study was to determine the effect of complete left unilateral ureteral obstruction on the antioxidant system changes in the contralateral kidney in male rats with an emphasis on the protective role of oleuropein.

In this experimental study, 18 male Wistar rats weighing 200 to 250 g were used. The animals were divided into 3 groups of 6 which included control, UUO, and UUO + oleuropein. The left ureter was ligated for 3 days. In the treatment group, the animals received oleuropein (200 mg/kg) daily for 3-days from one hour after ureteral obstruction. At the end of the experiment, the right kidney was removed and weighed. Then, the malondialdehyde (MDA) concentration, glutathione peroxidase (GPx), and superoxide dismutase (SOD) enzymes activity levels were measured in the right kidney.

UUO significantly increased right kidney weight and MDA concentration, and decreased the SOD and GPx enzymes activity level in the right kidney of UUO animals compared to the control (p<0.05). Oleuropein was able to reduce right kidney weight and MDA concentration in the UUO+oleuropein group compared to the UUO group (p<0.05), and significantly increased the SOD and GPx enzymes activity level (p<0.05).

UUO induces oxidative stress in the contralateral kidney, and oleuropein administration can have a protective effect on the contralateral kidney by restoring the antioxidant system.

 Expression of sexual desire is recognized as one of the important aspects of sexual life throughout the life course. Nevertheless, older adults experience many barriers to the expression of their sexual needs and desires. Thus, this study explored the barriers to the expression of sexual desires in the older ages.

A qualitative study with a conventional content analysis approach was conducted. Nineteen elderly males and females over 60 years participated in this study using purposive sampling and considering the utmost variety. Data were collected by semi-structured interviews until data saturation and concurrently analyzed via MAXQDA 10.

The decline of sex life, traces of the sufferings of life, sexually discouraging behaviors, and stereotypes in sexuality emerged from data analysis as the main considerations of expression of sexual desires in the older ages.

Sexual function in old age is a phenomenon affected by various factors with different natures. This phenomenon is overshadowed by physical condition, marital distress, stereotypical beliefs about sex in old age, and perceived feedback from the sexual partner. Accordingly, individual, cultural, and social interventions are recommended.

Leber congenital amaurosis (LCA) is a vision loss disorder that begins in infancy. Different genes are associated with LCA. They usually have autosomal recessive inheritance. In the present study, the genetic basis of congenital blindness in two Iranian families was examined.

technique was used for investigation of the underlying mutations in patients with LCA. The Sanger sequencing was employed to validate the identified potential pathogenic mutations.

In the present study, two novel mutations in GUCY2D gene were detected in probands. First mutation was a frameshift mutation caused by single base deletion (c.1264delC), and the second one was a nonsense mutation (c.2116C>T) resulting in stop codon. It was found that the carrier members were unaffected while the affected ones were homozygotes. Both mutations were confirmed using Sanger sequencing.

Herein, two novel pathogenic mutations were reported in Leber congenital amaurosis. This would be of importance in genetic diagnosis and consultation of patients affected with LCA.

Research is one of the important tools of decision making and planning which is one of the most basic foundations of community development. The purpose of this study was to investigate the motivational factors affecting research and its barriers from the perspective of faculty members and students at Jiroft University of Medical Sciences.

This cross-sectional study was performed on 154 students and 33 faculty members of Jiroft University of Medical Sciences in 2020. Participants in the study were selected by  convenience sampling. A researcher-made questionnaire including (demographic information, internal and external motivation factors, and inhibitory factors) was used to collect information. Data were analyzed using SPSS software version 20 at a significance level of p≤0.05.

A total of 182 people participated in this study. Among students, 99 (64.3%) were female, 137 (89%) were single, and 42 (27.3%) were medical students. 113 (73.4%) of them had a bachelorchr('39')s degree. 71 people (46.1%) had a history of participating in research workshops. Among the faculty members of the university, 54.5% were female and who 57.6% were assistant professors and 97% of the faculty members stated that they had participated in research workshops. 23.4% of students and 100% of faculty members had research experience. In both groups of students and faculty members, the interest in doing research with the frequency of 85.7 and 93%, respectively, obtained the highest score. In the external motivation of students and faculty members, respectively, the availability of appropriate research software in conducting research (83.8%) and providing healthy and deep knowledge (84.8%) had the highest frequency in research activities. Among the deterrents of students and faculty members, respectively, the ambiguity of the beginning of the research with a frequency of 67.5% and the weakness of teamwork with a frequency of 60.6% had the highest frequency.

According to the results, both faculty members and students were interested in conducting research activities but the ambiguity of the beginning of the research and the weakness of teamwork were the most common inhibitors. It is necessary for those involved in research domain to plan and take action to remove the existing obstacles while clarifying the research processes and supporting group research.

 Lung cancer is the second most common cancer in the world. The COR-L105 cell line is used as one of the most popular lung cancer cell lines in various studies. Long non-coding RNAs (lncRNAs) are a group of important cellular factors that play a key role in many processes such as growth, differentiation, replication, transcription, translation. LncRNA LINC00511 is a transcriptional regulator and has been reported to increase its expression in various cancers. The aim of this study was to knockout the LINC00511 gene using a CRISPR/Cas9 in COR-L105 cells and to investigate its effects on cancer progression and apoptosis.

 In this experimental study, two sgRNAs were designed for LINC00511 gene and cloned into two CRISPR vectors, separately. Two sgRNAs vectors were transferred to COR-L105 cells using lipofectamine. After confirmation of LINC00511 gene knockout from target cells, changes in cell proliferation and apoptosis were assayed by MTT and flow cytometry. Furthermore, the expression of genes associated with apoptosis and tumorigenesis was examined by real-time PCR.

LINC00511 gene knockout from COR-L105 cell line was performed. The expression of BCL2, survivin, EZH2, c-MYC, and MAX genes showed a meaningful decrease related to the expression in treated cells (edited) compared to the control cells (p˂0.05). Increased expression of p21 and p57 genes was also seen in the edited cells (p˂0.05). Decreased cell proliferation and increased apoptosis were observed in manipulated cells.

Knocking of LINC00511 gene in lung cancer cell line caused apoptosis and decreased cell proliferation. Therefore, inhibiting the expression of the LINC00511 gene in cancer cells leads to the control their proliferation.

Family functioning can be one of the most important determinants of mental health of prisoners. Despite the research that has been done on the relationship between family functioning and mental health, few studies have examined the relationship between these two variables among prisoners. The aim of this study was to investigate the relationship between family functioning and mental health of prisoners on the verge of release in Kerman and Yazd prisons in 2019.

 The study was a cross-sectional one, and the data collection tools were Family Functioning Questionnaire (FAD) and General Health Questionnaire (GHQ). The statistical population was all 743 prisoners on the verge of release in prisons in Kerman and Yazd provinces. The results were analyzed using SPSS 23 software and Pearsonchr('39')s correlation and linear regression.Out of prisoners, %95 were male and mean age was 27/9 ±15/37. In terms of marital status, 522 (70.3%) were married. Also, 378 (51%) of them had no history of imprisonment and 365 (49%) had a history of imprisonment of one to thirteen years. In terms of the type of crime committed, 335 (45%) of them committed drug-related crimes, 198 (27%) committed theft, and the rest (28%) committed other crimes. There was a positive and significant relationship between family performance and mental health of prisoners (p=0.001). The regression results also showed that %13 of the changes in the mental health of prisoners were a function of their family functioning. Also, among the dimensions of family functioning, three dimensions of problem solving (β=0.24), emotional intercourse (β=0.13), and behavioral control (β=13.13) were the strongest predictors of mental health of prisoners.

Family functioning components had a positive and significant relationship with all aspects of mental health except social inefficiency. The lower the level of family functioning, the lower the mental health of family members.

Cardiovascular disease is the leading cause of death in todaychr('39')s society, the main causes of which are a sedentary lifestyle and the use of high-fat and unbalanced diets. The aim of this study was to determine the effect of 4 weeks of aerobic exercise with octapamine supplementation on NLRP-3 gene expression and TRK-β protein synthesis in the heart muscle of rats fed with deep-fried oil.

30 male Wistar rats weighing 200-250 were randomly divided into 5 groups of 6: health control (CO), deep-fried oil (I),   deep-fried oil + aerobic exercise (H),  deep-fried oil +  octapamine, supplementation (B), and   deep-fried oil +  octapamine  supplementation +   aerobic exercise  (C). During the research period, 10 ml/kg deep-fried oil was given to the rats via gavage for 4 weeks. 81 mol/kg octapamine was intraperitoneally injected to the supplement groups. The rats in the exercise group also went through moderate intensity training in the first week (50% vo2max) and last week (65% vo2max) with treadmill.  One way analysis of variance was used to analyze the data and Bonferroni test was used to determine the difference, using Graph pad prism version 8.

NLRP-3 gene expression was significantly reduced in the exercise group compared to the control group who did not receive any supplements and exercise (p<0.05). Octapamine supplementation also decreased NLRP-3 (p<0.05). The combination of exercise and supplementation significantly reduced NLRP-3 gene expression compared with the control group (p<0.05). Changes in the amount of TRK-β protein in the exercise group did not show a significant difference compared to the control group that did not receive any supplements and exercise (p>0.05). In the group that also received octapamine supplementation, compared to the control group, TRK-β values did not change and did not show a significant difference (p>0.05). Combining exercise and supplementation significantly increased TRK-β gene expression compared to the exercise group (p<0.05).

Aerobic exercise training with octapamine supplementation may reduce the destructive effects of deep-heated oils on the expression of  the gene associated with myocardial degradation and improve remodeling in myocytes.

Electronic records represent the main source of services provided and patient health care. The purpose of this study is to evaluate the limitations of implementing electronic records of patients to improve the level of oral hygiene.

This study was a cross-sectional study. All faculty members (31 people) of Rafsanjan Dental School entered the study using census in 2020. Data were collected through a standard questionnaire. Data analysis was performed in SPSS-20 using descriptive statistics and Pearson’s correlation test. (p<0.05).

The age range of the respondents was 27-54 years. The highest and lowest mean and standard deviation of the restrictions on the implementation of electronic dental records were related to financial and managerial constraints (3.58±0.56) and attitudinal and behavioral constraints (2.99±0.53), respectively

In order to maintain oral and dental health, it is necessary to collect information in electronic dental records and remove the limitations of its implementation.