مجله سلول و بافت
سال دوازدهم شماره 2 (تابستان 1400)

The aim of the present study was to investigate the synergistic cytotoxicity effects of silver nanoparticles and bevacizumab against ovarian cancer cell line (A2780) and to analyze the expression of caspase 3 and Bcl-2 apoptotic genes.

The cytotoxicity of silver nanoparticles and bevacizumab at different concentrations including 62.5 to 1000 μg/ml and 1000 to 3000 μg/ml, respectively was investigated against A2780 cell line by MTT colorimetric method. Moreover, the expression of caspase 3 and Bcl2 apoptotic genes were studied using Real-Time PCR.

The results of this study showed that the silver nanoparticles and bevacizumab had dose-dependent toxicity and there were more significant cytotoxic effects than the single drug (p <0.05). Also, the IC50 values of silver nanoparticles and bevacizumab were 12.72±0.56 and 22.72±1.21 μg/ml, respectively. Also, the silver nanoparticles and bevacizumab changed the expression of caspase 3 and Bcl2 genes, respectively, which was significant compared to the housekeeping gene (p <0.05).

The results of this study showed that the silver nanoparticles-bevacizumab has a synergistic effect. However, more studies are needed to use this compound as a drug candidate.

The aim of this study was to investigate the effect of Cinnamic acid (CA) on some physiological characteristics and salt tolerance of tobacco (Nicotiana rustica L.)  plants.

In this study, explants of tobacco grown in MS medium was transferred to MS media containing CA (concentrations of 0, 5, 10 and 20 mg / l) and NaCl salt (concentrations of 0, 100 and 200 mM). After four weeks of treatment, the necessary analysis contains measurement activity of phenylalanine ammonialyase (PAL) and tyrosine ammonialyase (TAL), total phenolic compound and total protein, accumulation of flavonoid, anthocyanins, total antioxidant capacity, hydrogen peroxide (H2O2), potassium and sodium and proline done on the treated plants.

The results showed that the treatment of plants with CA increased the activity of PAL and TAL, total phenolic compounds and total protein. CA treatment also increased the accumulation of flavonoids, anthocyanins and proline as well as potassium and total antioxidant capacity. But the amount of sodium and H2O2 decreased as a result of CA treatment.

It is possible that CA increases the salt tolerance of tobacco by increasing the amount of total phenolic compounds as well as proline under salt stress conditions.

Buprenorphine as an alkaloid derivative of morphine is effective in the treatment of opioid dependence. Numerous reports on the beneficial and non-beneficial effects of buprenorphine have been reported in previous studies.

Alterations in the specific genes of protein kinase B (AKT), glycogen synthase kinase (3 (GSK3), brain-derived neurotrophic factor (BDNF), and binding protein CAMP (CREB) due to intraperitoneal injection doses of 10 and 6 mg/kg buprenorphine were evaluated during the a14-day period in the lumbar spinal cord of male rats.

Intraperitoneal injection of 10 mg/kg buprenorphine during the a14-day period increased the expression genes AKT and CREB genes in the lumbar spinal cord of male rats (P <0.05), while the applied doses of the drug did not significantly alter the expression of GSK3 and BDNF genes in this region.

By investigating the results of this study with the mechanisms proposed by previous researchers, it's probable that administration of high-dose of buprenorphine over a 14-day period, via the PI3 / Akt / CREB / BDNF signaling pathway, increased the expression genes of AKT and CREB in the lumbar spinal cord of rats and prevented from increasing the expression of adverse GSK3 gene. Also, the applied doses of the drug could not change the expression of the BDNF gene.

In the present study, the effect of different structural forms of plasmids on the efficiency of their transfer into the host was investigated. The toxicity effects of various forms of plasmids on host cells were also investigated.

Supercoiled, circular, nicked, and linear plasmid forms were separated from agarose gel. These structural forms were transfected to HEK293 cells and the transfection efficiency and toxicity of each of these forms was evaluated using the MTT technique.

The results showed that transferring the supercoiled form of pcDNA3.1+ plasmid into the host is much higher than other forms. Moreover, the toxicity effects of the linear form on the host are much higher than in other forms. Therefore, the results indicate that the supercoiled form of the plasmid is suitable for increasing the transfect efficiency and reducing cell death.

Researchers can use the supercoiled plasmid form in gene transfer processes to dramatically increase the efficiency of this process.

The purpose of this investigation was to induce callus and produce cell suspension from the well-known medicinal plant Nigella sativa (Black seed). Subsequently, the effects of salicylic acid and methyl jasmonate elicitors were investigated on their antioxidant content and potency.

Callus was prepared from the cotyledonary leaves of the plant. Then, cell suspension culture was established using appropriate callus. The cells were treated with salicylic acid and methyl jasmonate elicitors and the content of total phenol and carotenoids was measured. Antioxidant potency was measured by the FRAP method.

The results showed that the treatments had no significant effect on the fresh weight of callus but the dry weight of callus was significantly reduced by treatment with salicylic acid. The highest amount of total phenol was observed in callus culture and salicylic acid-treated cell suspension. Treatment of cell suspension with both elicitors significantly increased the amount of flavonoids. The treatments resulted in a significant increase in the antioxidant capacity of the cell suspension, which was less in the methyl jasmonate treatment.

According to the obtained results, it seems that in the cell suspension of N. sativa salicylic acid induces a greater response than methyl jasmonate, which leads to extra induction of antioxidant properties.

This study aimed to investigate the protective effects of different levels of vitamin A on ram sperm diluent during storage until equilibrium and before cryopreservation at 4 °C and freeze-thawing.

In this study, 4 Ghezel rams were used for semen collection by the artificial vagina. After the initial evaluation, the samples with normal properties were pooled and after the dilution process, 0, 5, 15, and 20 µmol vitamin A were added to the samples and frozen after 2 hours of cooling. Parameters of motility, viability, plasma membrane integrity, and sperm morphology were evaluated in two steps (before and after cryopreservation) as well as malondialdehyde levels after freeze-thawing.

The results showed that the addition of different levels of vitamin A to the extender during cooling at 4 °C had no significant effect on the evaluated parameters (P <0.05). The results after freezing showed that a level of 15 µmol of vitamin A increased the total motility, progressive motility (PM), VAP, and VSL (P <0.05). Plasma membrane integrity and viability increased significantly at the level of 15 µmol and also adding 15 µmol levels to the freezing medium decreased the amount of sperm with abnormal morphology and the amount of malondialdehyde compared to the control group (P <0.05).

According to the results of adding different levels of vitamin A, the level of 15 µmol has the best performance in improving the evaluated parameters and reduces oxidative stress during cryopreservation and thawing.