نشریه علوم درمانگاهی دامپزشکی ایران
سال پانزدهم شماره 1 (پیاپی 25، بهار و تابستان 1400)

Acute phase proteins (APPs) are a group of blood proteins primarily synthesized in the liver. Poultry respiratory diseases are one of the main causes of mortality and economic losses in the poultry industry. Antibiotics and symptom relieving agents are widely prescribed to control the condition. In this experimental study, the effect of different medicaments, including, Mentofin®, Aromex® and Biobron® alone (experiment ?) or in combination with antibiotics (experiment ?) on some APPs such as haptoglobin, serum amyloid A, ovotransferrin, adenosine deaminase, total serum protein, albumin and sialic acid (lipid- and protein- bound sialic acids) of viral respiratory complex (H?N? AIV+IBV) of broilers was investigated. Significant increase in serum concentrations of haptoglobin, serum amyloid A, ovotransferrin, sialic acid and adenosine deaminase were observed on days’ post infection in challenged groups. In experiment ?, treatments did not have significant effect on level of serum APPs in comparison with positive control group. In experiment ?, combination treatment by enrofloxacin did not have any significant effect on serum AAPs concentration. Overall, our results indicated that despite of widespread using of herbal essential oils on treatment of respiratory disease complexes due to infectious bronchitis and influenza viruses, did not have significant effect on measured APPs. In conclusion apparently these herbal medicines alone or in combination with enrofloxacin could not affect the pathogenesis pattern of the disease in relation to APPs production.

Bovine leukemia virus (BLV) is an oncogenic B-lymphocytotropic retrovirus and structurally related to the human T cell leukemia virus type ? (HTLV-?). Some haematological changes have been reported within the different stages of BLV. Altered blood enzyme profile, as well as biochemical parameters were also reported by different researchers. The aim of the present study was to biochemical evaluation of cattles serum infected with bovine leukemia virus. The experiment included ?? cows at various ages and body weight.On the basis of haematological results, the cows were divided into two groups: first group –BLV seropositive with normal lymphocyte; second group – BLV seropositive with increase lymphocyte. blood samples were collected from the jugular vein for Biochemical evaluation of cattles serum such as AST, GGT, calcium, Phosphor, BUN and Creatinin. Results indicated that The serum Phosphor concentration of persistent lymphocytosis (PL) Holstein cows (?.??±?.??) was significantly higher than that Non persistent lymphocytosis Holstein cows (?.??±?.??). Also, the The serum Calcium and aspartate aminotransferase (AST) concentration of persistent lymphocytosis (PL) Holstein cows (?.??±?.??) and (??.??±?.??) was significantly lower than that Non persistent lymphocytosis Holstein cows (?.?±?.??) and (??.??±?.??). There were no statistically significant changes in the other studied blood biochemical indices. Based on the result of present study, could be concluded that changes in some biochemical parameters in cattle infected with enzootic bovine leukosis virus in differentiation of bovine leukosis stages.

Newcastle is a highly contagious and deadly disease in the bird species, including partridges. Vaccine administration is one of the most important indicators of disease management. On the other hand, immune responses could be influenced direct or indirect by different herbal ingredients. In this study ??? Chukar partridges were divided into ?? groups. Then, live (B?, Clone and Lasota) and inactivated Newcastle disease vaccines were used at ?, ?, ??, ?? and ?? days of age by spray, eye drop, drinking and injection methods. The birds were fed with diets containing different levels of rosemary powder (?.?, ?, ?.? %) till to ?? days of age as starter diet. Next, blood samples were collected from the wing vein of the birds at ??, ?? and ?? days of age, and antibody titer was calculated by using Hemagglutination Inhibition (HI) test. Treatments that were vaccinated with live and inactivated vaccines, demonstrated higher antibody production compared to live vaccines alone (P

The present study was carried out to provide more information about proteins in uterine secretions in the camelus dromedarius. Camel ovaries, blood samples and uterine secretions from 50 sexually mature non-pregnant camels (C. dromedarius) were collected at slaughter. The total protein values of uterine secretions were determined by Lowry method and electrophoresis of proteins on polyacrylamide gel was carried out. Significant fractions of camel uterine proteins were determined. Standard molecular weight comparison revealed that the major protein in uterine fluid had a relative molecular weight of 70 KD and could be albumin. The nearly 30 KD band could be uteroferin and 13-14 KD band corresponded with 14 KD basic polypeptide of a member of plasmin/trypsin inhibitors. On the other hand, the nearly 44 and 23 KD bands were luteal-stage specific. Interestingly the nearly 23 KD luteal phase specific band in this study corresponds with the range of progesterone dependent retinol binding acidic proteins. Although camel uterine proteins are shown to be irrelevant of serum steroids, but the presence of two luteal phase specific bands showed that proteins in uterine secretions may change when the corpus luteum present

In this study, the effect of Securigera securidaca (Securigera S.) seed powders on growth performance, relative weight of some organs, serum and blood parameters, expression of Acetyl-CoA carboxylase (ACC) and Fatty acid synthase (FAS) genes in the liver and antihyperlipidemic properties of this seed in feeding broilers were investigated. A total of ??? day-old broilers (Ross???) were used in a completely randomized design with ? treatments, ? repetitions (?? pieces per pen) for ?? days. The experimental diets included a control group (without Securigera S. seed powder) with four levels of ?.?, ?, ?.? and ?? g/kg of Securigera S. seeds. The results showed that the dietary inclusion of Securigera S. improvement in body weight gain and reduced feed conversion ratio (P

Tissue engineering is a new way of replacing degraded tissue components with biodegradable polymers, which is provided as a three-dimensional scaffold for stem cell growth and proliferation. In the present study, effect of chitosan scaffold and the hyaluronic acid (HA) effects on wound healing in rats was used to evaluate. For this purpose, ?? rats weighing ??? to ??? g were prepared and divided into three groups of twenty. After surgical preparation, the skin of each animal's in spinal cord region completely thickeness with a diameter of ? mm by skin punch was removed. The Group ? was considered as negative control, the group two chitosan and in the group three, chitosan with hyaluronic acid was used. Then, at ?, ?, ? and ?? days after surgery, the lesions were examined macroscopically. Then, for histopathological evaluation, tissue sections were prepared from the lesion sites and the healing process was evaluated. The results showed that the wound area in the chitosan + HA group was significantly less than the control group on the third and ninth day after wound healing. The wound area on the ??th day was significantly lower in the chitosan and chitosan + HA groups than the control group. Histologic results of wound healing in terms of collagen formation, inflammation, vascularization, granulation tissue and epithelial remodeling showed improvement in healing process in chitosan and chitosan groups with hyaluronic acid compared to control group. The chitosan + HA group was better than the chitosan group. In general, according to the results, chitosan scaffold with hyaluronic acid can accelerate wound healing, epithelialization, increase angiogenesis and collagen formation and reduce inflammation. And can be referred to as biological dressing.Keywords: Chitosan, Hyaluronic Acid, Wound Healing, Rat

This study was conducted to investigate some blood factors of Liza abu in two winter and summer seasons in Musa creek at Petrochemi, Ghanam, Zangi, Douragh, Patil and Sajafi as a control station. After catching fish by trawling, ?? pieces of fish were removed almost equally. In each turn, fish samples were anesthetized with ?ml of clove extract per liter of water and blood samples were taken immediately from the caudal vein using a heparined syringe. Then, the parameters were measured by conventional and usual laboratory methods and the mean of them was determined in two seasons at each station and analyzed statistically. Results showed that the number of white and red blood cells, hematocrit percentage, hemoglobin amount, mean corpuscular volume (MCV), mean corpuscular hemoglobin (MCH) and mean corpuscular hemoglobin concentration (MCHC) in more pollution stations such as Petrochemi and Ghannam were significantly reduced compared to the control station (P

This study was conducted to investigate the hematological and biochemical changes caused of Eimeria ahsata during to 42 days of post-infaction. Twelve Lori-Bakhtiari lambs were provided and randomly divided into the Two groups after confirmation of their healthy. The animals in the experiment group were orally infected with 1× 10 5 sporulated oocysts Eimeria ahsata. From day 7 post-infection, the faeces were sampled separately on a daily basis and the number of oocysts per gram of faeces (OPG) were counted by McMaster method and were recorded for each lamb. The blood sampling recording was done on days 7, 14, 21, 28, 35 and 42 post-infection. The blood samples containing EDTA were examined for hematological and sample without EDTA, after centrifugation and isolation of serum, were examined for analysis of the biochemical parameters. The serum levels of ALB (Albumin), ALP (alkalin phosphatase), ALT (alamin aminotransferase), AST (aspartate aminotransferase), GGT (gamma glutamyltransferase), PRO (protein total) and CBC (Cell Blood Counter) were measured. the highest rate of oocyst excretion was observed in 32-33 days after infection. The CBC findings indicated that the mean number of lymphocytes and neutrophils were increased in the infected group compared to the control group on day 28 post-infection and The activity of ALB, ALP, ALT, AST, GGT enzymes as well as PRO showed no significant different between two groups Based on the results of the present study. Amongst the hematological, biochemical factors and OPG and CBC only showed significant difference between the control and the infected groups.

Endometritis is a local uterine infection without systemic symptoms that is manifested by the presence of purulent or muco purulent secretions in the uterus. This disease is a risk factor in ovarian cyst formation, anestrus and other genital problems. This disease increases service per conception and thus prolongs calving interval and decreases calving rate and ultimately, these uterine disorders lead to decrease genital function.

In this study, ??? cows with endometritis were selected and evaluated for histopathological and bacterial culture. According to the results of this study, out of ??? samples, ?? samples were reported as endometritis.

?? samples on Blood agar and ?? samples on Macconkey agar appeared positive. In terms of bacterial contamination, the bacteria found were predominantly Gram-positive. The highest level of contamination was observed in the samples that were simultaneously infected with two strains of S.Epidermidis and S. Aureus. There was a statistically significant difference in the frequency of bacterial strains. Histopathologically, differences in endometrial thickness, extent of endometrial gland scatter, extent of epithelial cells solidarity, endometrial blood vessel frequency and cell density were statistically significant. The blood agar culture was better than Macconkey culture because the high number of Gram-positive samples had grown there. The most effective antibiotics in this study were aerobic bacteria isolated from the uterus, was gentamicin.

The global spread of Newcastle disease virus (NDV) in poultry has highlighted the need for improved immunization programs against the disease. In this study, the effect of hemokinin-? (HK-?) as a biological agjuvant on the induction of humoral and cellular immune responses against chitosan-based NDV nanoparticles was investigated.

In this experimental study, , sixty SPF chickens were equally divided into six control and treatment groups. Control groups included received physiological serum, NDV inactivated antigen, and oil-adjuvanted NDV inactivated antigen; and treatment groups received chitosan, chitosan-based NDV nanoparticles, and the nanoparticles with HK-?. Based on the groupings, the chickens received each sample via eye drop on one day of age. Serum samples were collected at defined intervals and the humoral immune response was assessed by hemaagglutination inhibition (HI) test. Stimulation of the cellular immune response in different groups was assessed using cutaneous basophil susceptibility response (CBH).

Addition of HK-? to NDV nanoparticles increased the mean HI antibody titer compared to the group receiving nanoparticles alone. The difference in immune response between these two groups was significant (P

In this study sedative effect of Clove extract and Tobacco extract and tricaine methane sulfonate (MS???) on some organs of common carp (liver and gills) was studied. ??? common carp were divided to ? groups (? Clove extract ?? mg/l and ?? mg/l, ? Tobacco extract ?? mg/l and ?? mg/l and ? tricaine methane sulfonate (MS???) ?? mg/l and ?? mg/l for ? days and one group considered as control of study). Each group had ?? fish with ? replicates. In days ? and ? after sedation ? fish from each treatment were randomly selected and sampling was carried from the liver and gill. After fixation with ??% Formalin and routine procedures were performed in histopathological lab and Preparation of histopathological sections and staining sections, slides were examined. Histopathological examination revealed necrosis of lamella epithelium in Tobacco extract ?? mg/l group. In other groups of clove extract and methane sulfonate trichinae (MS???) no changes depend on this research were not seen in the liver and gills of fish. According to the microscopic results, clove extract has less pathological effects in comparison with Tobacco extract. More Histopathological research is definitely needed on the use of tobacco extract and clove extract in Aquaculture.

To determination chronic fluorosis in sheep, vicinity of Arak Aluminum factory, 100 sheep from two age group (under 2 years old and over 2 years old) from 0-10 kilometer from Arak aluminum industry (80 sheep) and 2 control group from 30 and 300 kilometers (20 Sheep) from Arak aluminum factory selected and plasma samples were collected. Plasma samples were gathered at four seasons and sheep feedstuffs (pasture and harvested forages) and water samples from different sources were analysis to measuring flour levels. Plasma and feedstuffs samples were analyzed for flour levels by ion selective electrode (ISE) and water samples were analyzed by ion chromatography (IC). Mean of plasma flour levels in sheep with dental lesions were 3.15±1.98 ppm and significantly higher than both control groups (0.39±0.13 ppm) (P=0.001). Two age groups were not shown remarkably difference in plasma flour levels (P=0.49). Various distances from factory had significant difference (P=0.001) in flour plasma levels and sheep within 0-10 km had higher plasma levels (3.15±1.98 ppm). In four seasons of year, sheep at fall (6.39±0.35 ppm) had higher flour levels than other season (P=0.001). In various water sources (Aqueduct, creek, fountain and pipe water) for sheep, creek (0.89± 0.31 ppm) had higher flour level than others (P=0.001). Feedstuffs analysis were shown pasture forages (79.15±50.38 ppm) had very higher flour levels than harvested forages (9.44±4.14 ppm) (P=0.001). Based on these study findings, chronic fluoride toxicity had occurred around (10 km) Arak aluminum industry in sheep herds and contaminated pasture had major role in occurrence of fluorosis in sheep. Further studies recommend to determining more risk factors of fluoride toxicity in this area.