مجله علوم گیاهان زراعی ایران
سال پنجاه و دوم شماره 2 (تابستان 1400)

To optimize tomato (Solanum Lycopersicum) plant tissue culture condition, the effect of 13 different hormonal combinations and two explants of cotyledon and hypocotyl on the regeneration rate of three Newton, Infinity and Defines cultivars were investigated. Experiments were conducted in a factorial based on a completely randomized design with three replications. The highest shoot number (11.9 shoots per explant) was obtained for Defines cultivar on MS medium containing the hormones of TDZ=0.68, NAA=0.1milligrams per liter and cotyledon explant. Plant defense responses against environmental factors are one of the inhibitor factors of explants regeneration in plant tissue culture. Calcium ion signaling is one of the primary events and, of course, the most important event in plant defense response against pathogens, Therefore, the effect of verapamil as a calcium channel blocker was investigated on the plant defense response. For this purpose, inoculation was performed using Agrobacterium tumefaciens strain LBA4404 and based on optimized tissue culture condition. The effect of verapamil in four concentrations of 0, 50, 100 and 200 milligrams per liter was investigated in a co-culture medium. Results showed that the use of verapamil in comparison with the control sample reduced the plant defense response. Also, the use of 50 milligrams per liter of verapamil increased the amount of regeneration.

To investigate the effects of fertilizer regimes on quantitative and qualitative traits of Dracocephalum kotschyi Boiss, a field experiment at three replications with the Split Plate arrangement in a completely randomized block design was conducted in the Research Field of Tarbiat Modares University during the 2017-18 growing season.Water deficit stress (irrigation after discharging 20 (optimal irrigation), 40 (mild water deficit stress), 60 (moderate water deficit stress) and 80% (severe water deficit stress) of water used (available water)) in the main plot and fertilizer regime (urea, nitroxin, vermicompost, azocompost and no fertilizer) in the subplot were studied. The results showed that the highest leaf fresh weight (1797 kg ha-1) and total biomass (2957 kg ha-1) were obtained by application of urea in mild water deficit stress condition and the highest dry matter digestibility percentage was observed in azocompost treatment in moderate water deficit stress condition (57.8%). The highest crude protein was produced in severe water deficit stress condition (19.72%) and moderate water deficit stress (18.4%), respectively.. The highest percentage of water soluble carbohydrate and neutral detergent fiber were obtained from no fertilizer in moderate water deficit stress (22.06%) and application of urea in severe water deficit stress conditions, respectively. Mild water deficit stress application to produce high biomass is recommended for Dracocephalum kotschyi Boiss. According to the results, application of urea in mild water deficit stress was selected as the best treatment to produce forage 32% more than control.

To evaluate the effect of nitrogen and water withholding in vegetative growth stage of corn on efficacy of nicosulfuron and 2,4-D+ MCPA an experiment was conducted as a factorial arrangement in a randomized complete block design with three replications at Tarbiat Modares University in 2017. The first factor contained four treatments including application of nicosulfuron, 2,4-D+ MCPA, weed free and weed infested as control. The second factor consisted of normal irrigation and water withholding from 4 to 8 leaf stage. Third factor contained either urea application or no fertilizer application. Normal irrigation and nitrogen fertilizer application significantly increased nicosulfuron and 2,4-D+ MCPA efficacy in weed control and total weed dry weight decreased to 341 and 361 g.m-2 respectively compared 1370 g.m-2 as weed infested. Nicosulfuron and 2,4-D+ MCPA reduced weed total dry weight significantly under irrigation withholding. Grain yield increased with application of nitrogen and nicosulfuron. Grain yield was higher with application of 2,4-D+ MCPA than nicosulfuron without nitrogen application under irrigation withholding. Application of nicosulfuron with nitrogen application provided satisfactory weed control and increased grain yield.

In order to investigate the possibility of quinoa producing in Garmsar, Iran, a factorial experiment conducted in randomized complete block design with three replications in 2018-2019 growing season at Garmsar Agricultural Research Station. The factors were planting date at three levels (March 6th, April 1st and April 6th) and the three genotypes of quinoa (Q26, Q29 and Titicaca). Results showed that the effect of planting date was significant for all studied traits except the harvest index. Also, all studied traits were significantly different in all genotypes. Titicaca planted on March 6th had the highest yield (2276 kg.ha-1).The grain yield and yield components decreased with the delaying the planting date. Compared to early plantings, Latest date, April 6th, led to reduction of all traits, especially grain yield (about 50%). The results of simple phenotypic correlation between the studied traits showed that grain yield per hectare had the highest correlation with plant yield (0.877) and then with leaf area index (0.832), panicle weight (0.815) and number of branches per plant (0.745) that was significant at the 1% probability level.

Water Water deficit especially after heading is one of the most effective factors of wheat yield loss in Iran and across the world and identifying the physiological traits related to plant resilience is of great importance in screening for tolerant varieties. The objective of this study was to evaluate the variation in stem reserves accumulation capacity and its relationship with remobilization in some wheat cultivars. 22 wheat cultivars were cultivated in a split plot design with three replications, full irrigation and 40% of field capacity being main plots. The amount of accumulation and remobilization was estimated by measuring internodes weight and stem soluble carbohydrates content (WSC). Significant genotypic variation among cultivars was observed under both normal and drought conditions. Maximum accumulation was found in lower internodes followed by peduncle and penultimate using dry weight measurement method. In WSC measurement method, however, maximum accumulation was observed in penultimate followed by lower internodes and peduncle. Cultivars ranking based on accumulation was also different in two methods. Drought stress caused decline in internodes weight, WSC and yield and remobilization efficiency. The specific stem weight had the highest correlation with remobilization. Significant correlation was found between grain yield and remobilization, specific stem weight and WSC.

Identifying the constraining factors of production and yield gap is very important. Therefore; this research was performed to identify the production constraining factors of local rice cultivars. All management practices from nursery preparation to harvesting stages for 100 paddy fields of local rice cultivars were recorded through field studies, in Sari, from 2015-2016. In the CPA, the actual and calculated potential yield were 4495 and 5703 kg/ha, respectively and the gap was 1221 kg/ha. The yield gap caused by number of top-dressing variables was 324 kg/ha, equal to 27% of the total yield gap. The yield gap related to previous year of legumes cultivation was 218 kg ha-1, equal to 18% of the total yield variation. Among the 10 variables entered in the CPA model, the effects of top-dress fertilizer application and its application frequency and foliar application were remarkable, which could compensate a significant part of the yield gap (444 kg/ha, 37% of total) in the farmers’ fields by managing these variables. According to boundary line analysis (BLA) finding, actual yield mean on the basis of optimal limit related to 12 variables under study was 5369 kg/ha, with 881 kg/ha yield gap . Mean relative yield and relative yield gap for 12 variables (transplanting date, seedling age, number of seedlings per hill, planting density, nitrogen and phosphorous per hectare, nitrogen before transplanting, harvesting date, lodging problem, pest problem, diseases problem and weeds problem) were 83.64 and 16.35 kg/ha, respectively. Based on the finding, it can be stated that the model precision is appropriate and can be applied for both estimation of the quantity of yield gap and determining the portion of each restricting yield variables.

To investigate the effect of vermicompost, N fertilizer and their integration on growth, biological and essential yields oil and its components in two populations (Varamin and Isfahan), an experiment was conducted in a randomized complete block design with three replications in the field of Isfahan Medicinal Plants Research Center in 2016. Fertilizer treatments including 100% urea, vermicompost 33.3 and urea 66.6%, vermicompost 66.6 and urea 33.3%, 100% vermicompost and control treatment (without fertilizer) as the first factor and Varamin and Isfahan populations as the second factor were selected. The results showed that the interaction effects of nitrogen fertilizer and populations on all studied traits were significant. In both populations, nitrogen fertilizer improved height, biological and essential oil yields, content of essential oil compounds (d-Carvone and its yield, α-phellandrene and linalool; except p-Cymene and Limonene in Isfahan population). Since in medicinal plants, the quantity (biological yield) and quality (essential oil and d-Carvone; the most important ingredient and the highest amount of essential oil in this experiment), 66.6% vermicompost + 33.3% urea in Varamin population, was the best treatment in the experiment. In addition an in the direction of human health and sustainable agriculture, it is possible to reduce 33% of chemical fertilizers application and pollution; however, in terms of other essential oil contents (α-phellandrene, Linalool and p-Cymene), 100% vermicompost fertilizer treatment was superior in Isfahan population.

Recognition and understanding the genetic control of traits, combining ability and genetic structure are directly related to the success of breeding programs. For this purpose, a 7 × 7 one-way diallel design was conducted in a randomized complete block design with three replications. The measured traits were included plant height, height to the first capsule, number of days to 50% and 90% of flowering, number of capsules per plant, number of seeds per capsule, number of days to physiological ripening, number of branches, leaves number and length, 1000-seeds weight, capsule weight, length and width, chlorophyll a, b and total chlorophyll, biological andeconomic yields, harvest index, oil and protein percentage. Analysis of variance showed that there was a significant difference between genotypes and diallel analysis showed that the additive variance of all traits and dominant variance of all traits except height to the first fruit-bearing capsule were significant. The oltan cultivar was the best and Ardestan genotype was the worst genotype in terms of general combining ability. Sabzevar×TS-3 and Sirjan×Fars were the best hybrids in most traits. The general heritability was between 0.90 to 0.96 for biologic yield and number of branches, respectively and narrow heritability was between 0.36 to 0.91 for the number of branches and harvest index, respectively. The analysis of variance by Hayman method confirmed the results of Griffing analysis.

To investigate the response of corn to combined application of chemical fertilizers with rhizobacteria plant growth promoting, an experiment was conducted in 2017 at Research farm of Agricultural and Natural Resources Campus of Tehran University, Karaj, Iran, in a randomized complete block design with four replications. Four nutritional treatments including T1 (Control treatment without applying fertilizer), T2 (Just PGPRs), T3 (Use chemical fertilizers based on soil test) and T4 (T3 + PGPRs) were considered. According to the results, the highest total dry weight (3.9 kg/m2), crop growth rate (79.8 g.m-2.day-1), net assimilation rate (15.3 g.m-2.day-1) and grain yield (18.2 ton.ha-1) were observed in T4 treatment and T2 treatment produced the highest  leaf area index (5.3), leaf area duration (205.2) and specific leaf weight (78.5 g.m-2) . Also, the lowest value of all traits was observed in in T1 (control) treatment. The results showed that the presence of rhizobacteria plant growth promotioninduction in the corn nutrition program increased the growth and growth indices of the plant. Combined application of chemical fertilizers with rhizobacteria plant growth promoting resulted in the highest growth and final grain yield of corn.

In order to identify the correlations and relationships between different traits in the bread wheat, 30 genotypes were investigated as sub-factors under the split plot experiment in the completely randomized design in greenhouse and the randomized complete block design in the field with three replications under the normal irrigation and post-anthesis water stress conditions. The results of the canonical correlation analysis showed that at both normal and moisture stress levels, there was a significant correlation between the pair of canonical variables obtained from the phenological traits and the yield components. Under the normal conditions in the greenhouse, to increase the spike density and length, the number of days to 50% flowering can be considered as a suitable selection criterion. According to the results of canonical correlation analysis, under the stressed conditions in greenhouse, to increase the spike length, the number of days to the heading, maturity and 50% of flowering were among the important and effective factors. Under the optimum irrigation conditions in the field, the number of days to maturity was considered as one of the factors influencing the seed weight per spike. The results of canonical correlation analysis under the field stress conditions showed that to increase the 1000-seed weight, the number of days to maturity can be considered an appropriate selection criterion.

To evaluate the effects of different treatments on seed dormancy breaking of Notobasis, two separate experiments were conducted at Agricultural Sciences and Natural Resources University of Khuzestan, in 2017 with three replications. The first experiment included nine physical, mechanical and chemical treatments (control, surface scarification, intense scarification, one and two weeks of chilling at 4°C, soaking into hot water for two hours without scarification , soaking in 25°C distilled water for two hours with scarification  and acid treatment for six and 12 minutes) based on completely randomized designs (CRD). The Second experiment was hormone priming arranged as a factorial experiment based on completely randomized design with gibberellin concentrations (0, 200, 400, 600, 800 and 1000 ppm) and priming duration (12 and 24 hour) as the treatments. The highest seed germination (40%) obtained from 12 minutes of acid treatment. Results showed that seed germination reached to 100% after priming seeds with GA3 concentrations for 12 hours. However, increase the priming durations to 24 hours, significantly declined seed germination to 47% in 200 and 400 ppm of GA3 concentrations. The Increase of GA3 concentrations in 12 hours led to a linear reduction of T50 from 31 hours in 200 ppm to 19 hours in 1000 ppm). In 24 hours of priming treatment, T50 followed a Gaussian function and the highest T50 (92 hours) was obtained from 600 ppm. It is recommended to immerse the seeds for 12 minutes in sulfuric acid and prime with 400 ppm GA3 acid for 12 hours to break dormancy and increase the vigor index.

This study was conducted during summer and winter of 2018- 2019 in the agricultural research field of Shahid Chamran University. Experimental design was split- plot based on RCBD with three replications. The main plot was the type of agricultural system in three levels including conventional (Conv), organic (Org) and sustainable (Sust) (integrated between Conv and Org) and sup- plot was the type of pre- cultivated crop in sequence with wheat including cultivation of mung bean (M- W), corn (C- W), sesame (S- W) and fallow (F- W). Yield quantity (yield and its component) and quality (grain protein), an estimate of photosynthesis matter transfer index of wheat and soil organic carbon (SOC) after one double-cropping were measured. The result showed that the highest (545.04 g/m2) and the lowest (409.28 g/m2) seed yields were obtained in Conv and Org respectively. In contract, with the changing type of system from Conv to Org, grain protein was increased significantly (from 8.3 to 9.6 %). In addition, the highest (535.47 g/m2) yield of wheat was obtained from M- W double cropping. On the other hands the highest remobilization and current photosynthesis matter were obtained in the organic agricultural system with M- W and conventional with M- W double cropping. The situation of SOC showed that the highest (33.18 mg/g) SOC was obtained in the organic agricultural system with C- W double cropping. The reason for improving SOC in the organic and sustainable agricultural system was application of organic matter (compost and vermicompost) and crop residue management. Totally, from the crop ecology point of view, sustainable agricultural method with a sequence of M- W was the most desirable system.

To investigate the effects of water deficit stress and bio and non-bio fertilizers on Thymus Vulgaris, a split plot experiment was conducted based on randomized complete block design with three replications in 2017 in Asgarya agricultural field in Yazd. The treatments included water tension: W1=7 (Control), W2=12 and W3=17 days irrigation intervals as the main factor and bio and non - bio fertilizers at five levels including B1= control (without fertilizer), B2= endo mycorrhiza (Glomus), B3= Azospirillum bacteria, B4= Pseudomonas bacteria and B5= chemical fertilizer (NPK) a sub – factors. Results showed that the highest flowering branches yield, a, b, and total chlorophylls and nitrogen and phosphorus elements were observed in 17 days of irrigation (W3). 12 days of irrigation treatment (W2) showed the highest potassium element rate. The highest a, b and chlorophylls and nitrogen were observed in Azospirillum treatment (B3). The highest total chlorophyll, phosphorus element, carotenoids and flavonoids were observed in Pseudomonas treatment (B4); the highest potassium element rate were obtained in mycorrhiza treatment (B2); the highest flowering branches yield were observed in NPK treatment (B5) and control (B1) treatment resulted in the highest anthocyanin.

Rapeseed duo to having genotypes spring and autumn and adaptation to weather conditions as the point hope for provide edible oil of country is the required. Two separate experiments were set up as split-plot in a RCBD design with three replications in two fall and winter seasons during two years (2014-2015 and 2015-2016) at the research field of Seed and Plant Improvement Institute to investigate the effect of sowing season and date on the quantitative and qualitative yields of rapeseed genotypes in Karaj, Iran. In this study, the main plots were sowing dates of 7, 17, and 27 October in fall sowing and sowing dates of 9 and 19 February, and 1 March in winter sowing and sub-plots consisted of genotypes of RGS003, Dalgan, Zabol10, Hyola401, and Hyola4815. Grain and oil yields of rapeseed genotypes were 4330 and 1840 kg ha-1 in fall sowing, respectively, while these traits were decreased by 43.3 and 46.6% when rapeseed genotypes were planted in winter season, respectively. The highest grain and oil yields (5033 and 2202 kg ha-1) belonged to 7 October in fall sowing, while 9 February had the highest grain and oil yields (2996 and 1248 kg ha-1) in winter sowing. In general, the Dalgan genotype is recommended for cultivation due to suitable agronomic traits such as number of silique per plant, number of grain per silique, silique length, 1000-grain weight, as well as high grain and oil yields in cold temperate regions with arid and semi-arid climates such as Karaj, Iran.

In order to investigate the effect of weed interference and weer-free periods on Balangu (Lallemantia royleana Benth. in Wall) and to evaluate the critical period for weed control (CPWC) in this oilseed crop, an experiment was conducted as a randomized complete block design with three replications in Naghadeh region in 2018. Experimental treatments consisted of two groups; weed infestation treatments (144.2, 345.9, 574, 848.2, 1147.6 and 1451.3 growing degree day (GDD) after transplanting and plots then remained weed-free the rest of the growing season) and weed-free treatments (144.2, 345.9, 574, 848.2, 1147.6 and 1451.3 GDD after transplanting and remained until harvest time). In addition, weed establishment at 1993 GDD represented the weedy (all season) treatment and weed removal at 1993 GDD represented the weed-free treatment. Results indicated that density and dry biomass of weeds increased as weed removal was delayed. In contrast, density and dry biomass of weeds decreased as weeds establishment was delayed. Height, tetrachenes fruits, ripened achenes, 1000-seed weight, biological yield, seed yield and relative yield of Balangu decreased and increased as the duration of weed-infested and weed-free period increased, respectively. Logistic and Gompertz curves fitted also showed that the beginning and end of the CPWC based on a 10% yield loss of relative yield, was estimated to be from 11 to 108 days after transplanting (97 days weed-free period) in order to prevent a noticeable yield loss.

To assesse the iron and silicon effects on the yield and physiological characteristics of green pea, Wando cultivar by ASGrow Corporation was used in three levels of iron chelate (0.05, 0.1 and 0.3 gr per l-1) and silicon (0, 14 and 28 mg per l-1), as a factorial experimental in completely randomized design with three replications. In this experiment, characterisics such as fresh and dry weight of grain, the number of grain per pod, concentration of Fe, Cu, Mn, Zn and Si in leaf and grain were measured. Results showed that the interaction of Fe and Si was significant at p<0.01 on grain fresh and dry wigeht, concentratin of leaf and grain Fe, leaf Mn, leaf and grain Zn and leaf Si and the application of Fe and Si was significant separately, on other traits such as number of grain per pod, concentration of leaf and grain Cu, Mn and Si in grain. The Mean squares showed that the application of Fe and Si significantly increased fresh and dry grain weight and Si laef concetration and decreased the concentratin of leaf and grain Fe and Mn as well as grain Mn. The enhancement of Fe and Si application separately decreased the concentration of Mn, Cu and zinc leaf and grains, but the Si concentration of leaf and grain were increased by Si application. The concentration of Fe, Mn, Cu and Zn were decreased in leaf and grain because of antagonistic effects. It can be concluded that Si reduces the harmfull effects of high levels of iron toxicity and the application of 0.1 g/l and 25 mg/l can be recommended as the best treatments.

To achieving favorable outcomes in breeding programs, parental selection based on General combining ability (GCA) and Specific combining ability (SCA) is important. In order to investigate the genetically characteristics and general combining ability and specific combining ability and disease-resistant genes action against take-all in bread wheat, 6 wheat genotypes (729, 1622, 2109, 1528, 1546, 1526) were crossed in One-way diallelcross. Seeds of crosses (F1s) and parents were planted in the research greenhouse of Vali-e-Asr University of Rafsanjan. the take-all disease tolerance, stem dry weight, root dry weight, tiller number and Mn, Zn, k and Fe concentrations Were measured. Griffing analysis showed that general combining and specific combining ability were significant for all traits except for tiller number and iron element. The best general combiner in viewpoint of take-all resistance were 1622 and 729. The best hybrids in viewpoint of disease resistant were 2109×1546, 546×1528 and 1622×1526 that has the highest specific combining ability. Evaluation of genetic parameters by Hayman method for disease index and disease score confirmed the results of griffing analysis and showed that the dominance and over dominance of gene’s actions have the greatest importance in genetic control of the resistance to take-all disease (T-41 isolation). Finally, due to low specific heritability and low genetic ratio in resistance to take-all disease, it can be said that this trait do not respond well in early generations so selection after purity can be effective and can be done by bulk, single-seed descent and double haploid methods in wheat breeding.

To study the effect of salinity stress on some physiological traits in wheat cultivars (Triticum aestivum L.), a factorial experiment based on randomized complete block design with three replicates was performed at 2017 in the research greenhouse of Agronomy Department, University of Tehran. Salinity treatments at two levels; control (1.2 dS/m) and sodium chloride treatment (16 dS/m) and seven wheat cultivars, Rowshan, Inia-66, Mahdavi, Khazar-1, Karaj-2, Ghods and Shole were the experimental treatments. According to the results, relative water content, yield and its components and potassium/sodium ratio decreased in all genotypes while Na+ content and remobilization of carbohydrates were increased. Salt resistant varieties, Roshan and Inia-66, had the highest level of relative water content, remobilization of soluble carbohydrates and number of grains per spike. In addition, Roshan cultivar showed the highest potassium concentration and K+/Na+ in the shoots, while Ghods and Sholeh as sensitive cultivars had the highest sodium concentration in the shoots. Findings showed that the ability of a cultivar to remobilize soluble carbohydrates is one of the traits that determine salt stress tolerance, hence, this trait is suitable for evaluating salt tolerance of wheat cultivars in the future investigations.