نشریه پژوهش هایی در کشت سلول و بافت کاریوتیک
سال دوم شماره 1 (بهار 1400)

Although several studies have been carried to investigate the effects of dandelion extracts on the viability of cervical cancer cells, the results on the effects of dandelion on cell viability, particularly in cervical cancer cells, are still challenging. The aim of this study was to investigate the cytotoxic effects of dandelion (Taraxacum officinale) extract on cervical cancer cells compared to non-cancerous cells.

In this laboratory experimental study, HeLa cancer cell line and non-cancerous embryonic kidney cells (Hek293) were purchased from Pasteur Institute, Tehran, Iran. Cells were divided into control and treatment groups. In the treatment group, cells were exposed to 0.002, 0.02, 0.2, and 2 mg/ml of dandelion flower extract. MTT assay was used to evaluate cell viability. Data were analyzed using one-way analysis of variance

Hela cancer cells viability was significantly reduced in the groups exposed to 0.2 and 2 mg/ml of extract compared to the control group (p<0.01 and P<0.001, respectively). None of the concentrations used had a significant effect on the viability of Hek293 cells.

Dandelion flower extract in appropriate concentrations can reduce the viability of cervical cancer cells without side effects on healthy non-cancerous cells. The findings of this study support previous research indicating the anticancer effects of dandelion on cancer cells.

Several studies have been carried out on the biological characteristics of a human adipose tissue-derived-stromal vascular fraction. However, investigating this fraction concerning its clinical application is still of significant importance. The aim of this study was to isolate and evaluate the cell population of human adipose stromal vascular fraction with the main aim of investigating the presence of stem cells in human adipose stromal vascular fraction.

In this laboratory-experimental study adipose tissues were obtained from 10 healthy individuals (30 to 58 years) and maintained in standard condition. After enzymatic isolation, the viability of stromal vascular fraction cells and surface antigens was evaluated by flow cytometry.

The results of this study showed that adipose stromal vascular fraction cells had high viability (> 98%) and were positive for CD16, CD34, CD73, CD29, CD105, CD31, and CD45 markers and negative for CD3, CD19, and CD38 markers.

The findings of this study indicate the presence of adipose-derived stem cells, endothelial cells, peri-adipocytes, and macrophages. According to this, this fraction has potential in the clinical field of cell therapy.

Human chorionic gonadotropin (hCG) is a glycoprotein produced during pregnancy by trophoblast and placental syncytiotrophoblast cells. The produced hCG hormone affects the corpus luteum and prevents it from being destroyed. Studies show that diamond nanoparticles (NDs) interact with biological systems such as proteins, enzymes, and antibodies. In other words, NDs are capable of being absorbed by biomolecules and can also be used as a carrier for the transport of biologically active substances. This study aimed to investigate the effect of NDs on hCG structure and on hCG level in Wistar rats.

The effect of 10 nm NDs on the structure of hCG was investigated using spectroscopic techniques. Rats were randomly divided into five groups: Control, sham, and experimental groups (received NDs solution at doses of 25, 75, and 100 mg/ml). Injections were carried out twice a week. Then, serum biochemical parameters were calculated. Data were analyzed utilizing one-way ANOVA followed by Tukey's multiple comparisons test.

The results showed that NDs could form a complex with hCG. The zeta potential and DLS results showed that NDs cause a decrease in the surface charge and size of hCG. Administration of NDs in concentrations of 100 mg/ml significantly decreased hCG levels compared with that of control samples (P<0.05),

Based on the investigation NDs could change the tertiary structure of hCG. NDs had toxic effects on hCG function. More studies to explore the toxic effects of NDs in various doses and times of exposure seem to be necessary.

Colorectal cancer is a common disease ranked as the third-highest deathly cancer worldwide. In recent years, for the treatment of cancers scientists have preferred herbal remedies because of their better effects and fewer side effects. So, in this study, the cytotoxicity effect and apoptosis induction of 7-hydroxycycoumarin were investigated on the SW480 colon cancer cell line. anti-proliferative effect of 7-hydroxycoumarin was investigated using Trypan blue and MTT assay at different concentrations on SW480 cell line. Additionally, apoptosis induction was examined by flow cytometry and real-time PCR. The results show a decrease in viability. IC50 concentrations were observed at the concentrations of 164.2, 80.48, and 41.97 μg/ml for 24, 48, and 72 h, respectively. Of note, IC50 concentrations were not observed in the trypan blue assay. As well, there was a 2.4-fold increase in apoptosis (P<0.001) using Annexin / PI kit by flow cytometry. as well as the increased expression of BAX/BCL2 ratio of about 13.69, which was observed at 100 μg/ml compared to the control. The results indicate that 7-hydroxycoumarin could inhibit cell proliferation and induce apoptosis in the SW480 cell line. Therefore, 7-hydroxycycoumarin can be considered as an anti-cancer agent in the complementary treatment of colon cancer.

This study was concentrated on the algae effects and compare it with the atorvastatin drug on the lowering of cholesterol and blood sugar.

In this research 24 native breed dogs were used for 30 days, the dogs were divided into three groups of eight, a group of control, a group orally receiving 2 gr Spirulina, and receiving a group of 5 mg/kg of Atorvastatin, and then blood samples were analyzed.

We observed mainly reduction and increase in blood glucose in Spirulina and atorvastatin receiver groups respectively (P= 0.001). Evaluation of lipid factors in dogs were declined in total cholesterol, Low-Density Lipoprotein levels, and Triglyceride factors (P<0.001). This research illustrated that the use of Spirulina in dogs for a short time could improve lipid and liver factors.

Considering that the results of the present study are showed that using Spirulina can be concluded without worrying about allergic reactions and its side effects. It can also be used as a replacement for chemical drugs.

The current study is provided for floristic research of Pinnulariaceae for the first time in Iran from 1972 to July 2021. The pinnulariaceae family contains 6 genera Pinnularia, Oestrupia, Hygropetra, Envekadea, Diatomella, and Craspedopleura which are two genera Pinnularia and Diatomella have been recorded in Iran. The purpose of the present study was to provide a checklist of Pinnularaceae in Iran ecosystems.

Different recourses were used to provide this paper, including the thesis of students' dissertations, books, and articles. The old taxonomic names of taxa were converted to new names based on algae-based websites.

This taxonomic list contains 2 genera comprises Diatomella and Pinnularia. It includes 52 species with emphasis on the Pinnularia Ehrenberg genus from various ecosystems. The Pinnularia was the most frequent genus with 51 species and 98% of the species in the Pinnulariaceae. Diatomella Greville was stand in the second genus in the frequency of population (2%) with 1 species. There are only one species of Diatomella in the current checklist of Pinnulariaceae which has been reported from Kordan River in Alborz Province.

Some of the species’ names have changed based on this update.