فهرست مطالب

Advanced Biomedical Research - Volume:12 Issue: 5, May 2022
  • Volume:12 Issue: 5, May 2022
  • تاریخ انتشار: 1401/03/18
  • تعداد عناوین: 9
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  • Shima Aboutalebian, Somaye Mirzaaghaei, Hamed Fakhim, Sama Faramarzi, Somayeh Mousavi, Safiyeh Ghafel, Sahar Gholipour, Armin Farhang, Hossein Mirhendi*, Mahnaz Nikaeen Page 37
    Background

    Early and cost‑effective diagnosis and monitoring of the infection caused by severe acute respiratory syndrome coronavirus 2 (SARS‑CoV‑2) are critically important to anticipate and control the disease. We aimed to set up a SYBR Green‑based one‑step real‑time polymerase chain reaction (PCR) as a lower‑cost alternative method to detect the virus.

    Materials and Methods

    An in‑house SYBR Green‑based PCR assay targeting the envelope (E) and RNA‑dependent RNA polymerase (RdRp) genes, was set up to diagnose the infection, and was compared with the reference probe‑based PCR method.

    Results

    When the commercial probe‑based assay was considered as the reference method, SYBR Green‑based PCR had a slightly lower sensitivity (81.98% and 86.25% for E and RdRp targets, respectively) and a good specificity (100% and 94.44% for E and RdRp targets, respectively). For both gene targets, three different melting temperature (Tm) patterns were found in the PCRs of the nasopharyngeal/oropharyngeal swab samples, but no size polymorphism was seen in agarose gel electrophoresis.

    Conclusion

    Further studies to improvement of the assay are needed to make it an inexpensive and reliable tool for the diagnosis of COVID‑19.

    Keywords: E‑gene, RdRp‑gene, SARS‑CoV‑2, SYBR Green, Diagnosis Biological Assay
  • Mehrdad Mohammadi Sichani, Reza Safi, Saeid Haghdani*, Mohammad Hatef Khorrami, Farshid Alizadeh, Mohammad Hossein Izadpanahi Page 38
    Background

    The current study aims to assess the effects of the large simple renal cyst (SRC) surgery on renal function for the first time.

    Materials and Methods

    This case‑series study included 22 patients with larger than 50 mm single SRC who underwent laparoscopic cyst unroofing surgery. Twenty‑four‑hour urinary protein, creatinine, and volume along with plasma creatinine and estimated glomerular filtration rate (GFR) were measured in patients before and 1 month after surgery. Patients underwent abdominopelvic computed tomography‑scan without contrast and parenchymal thickness diameter adjacent to the cyst was measured before and after surgery.

    Results

    Mean age and weight of patients were 52.2 ± 8.9 years and 77 ± 10.9 akg, respectively. There was no significant difference between plasma creatinine and GFR before and after surgery (P = 0.25 and 0.37, respectively). Twenty‑four‑hour urinary volume, creatinine, and protein before and after surgery revealed no significant changes (P = 0.37, 0.08, and 0.31, respectively). The mean improvement of parenchymal thickness diameter after surgery was 10.4 ± 0.7 mm which was statistically significant (P = 0.001). However, it was not correlated with the mean estimated GFR change (r = 0.349, P = 0.13).

    Conclusions

    Although laparoscopic unroofing of renal cyst recovered renal parenchymal thickness noticeably, it did not improve renal function significantly; therefore, the latter factor lonely should not be considered a reason for surgery decision. Observation can be chosen as a safe treatment strategy in large SRCs without concern about renal function

    Keywords: Cystic Kidney Disease, glomerular filtration rate, Kidney Function Tests, Laparoscopy
  • Fahimeh‑Sadat Jafarian, Gillian Yeowell, Ebrahim Sadeghi‑Demneh* Page 39
    Background

    The assessment of the emotional consequences of having a spinal deformity and orthotic management in people with idiopathic scoliosis is essential. This study was aimed to translate and culturally adapt the Bad Sobernheim Stress questionnaire (BSSQ‑Brace) for Iranian adolescents with idiopathic scoliosis using thoracolumbar orthoses.

    Materials and Methods

    The BSSQ was translated into Persian according to a standard guideline. The Persian BSSQ‑Brace was completed by 55 Iranian subjects with spinal deformities who agreed to participate in this research. The validity was determined by correlating the Persian BSSQ‑Brace questionnaire (BrQ) and the revised Scoliosis Research Society‑22 patient questionnaire (SRS‑22r). To assess test‑retest reliability, all participants completed the BSSQ‑Brace twice, with a gap of 1‑week. Intraclass Correlation Coefficient and Cronbach’s alpha were measured to report reliability.

    Results

    All population revealed items in the Persian BSSQ‑Brace were easily understood, and there was no difficulty completing them. The psychometric properties of the Persian version of BSSQ‑Brace demonstrated excellent test‑retest reliability (interclass correlation coefficient = 0.94), excellent internal consistency (Chronbach’s alpha = 0.94), and high construct validity with SRS‑22r (r = 0.74).

    Conclusions

    The Persian BSSQ‑Brace is a reliable and valid instrument to assess the emotional domains in subjects wearing a spinal orthosis.

    Keywords: Emotions, orthotic devices, scoliosis, spinal curvatures, surveys, questionnaires
  • Kazem Ghaffari, MohammadAmin Aghajari, Ali Ghasemi, Yazdan Ghandi, Vahid Falahati* Page 40
    Background

    The exact prevalence of hypertension in children surviving acute lymphoblastic leukemia (ALL) has not been fully estimated. The aim of this study was to investigate the prevalence of arterial hypertension (AH) and to determine the risk factors for the development of AH in children surviving ALL with current treatments.

    Materials and Methods

    A total of 150 patients (84 males, 66 females, with an age range of 1–16 years) were included in the study. Demographic and clinical information of patients were initially recorded. Hypertension is defined as average systolic blood pressure (BP) and/or diastolic BP that is greater than the 95th percentile for gender, age, and height.

    Results

    The mean age at the assessment of BP was 11.3 and 9.8 years in the ALL and control group, respectively. A total of 20.6% of survivors of ALL and 10% of controls had high BP. Most patients in both groups had normal BP (65.3% patients in ALL group and 75.4% subjects in the control group). The number of patients with hypertension was significantly higher in ALL patients as compared with the control group (P = 0.026).

    Conclusion

    The prevalence of AH in children surviving ALL is higher than in children in the general population, which emphasizes the need for regular monitoring of BP in children surviving ALL and intervention in the lifestyle of this population. Careful follow‑up of BP status is warranted for long‑term survivors of childhood cancer.

    Keywords: Acute lymphoblastic leukemia, Children, Hypertension, Survivors
  • Masoud SayadiShahraki, Mohsen Mahmoudieh, Behrooz Keleidari, Hamid Melali, Abdulhakim Algazali* Page 41
    Background

    Patient management after bariatric surgery is important in controlling patients’ diabetes and recurrence prevention. This study aimed to meet the medical managements of patients with diabetes 6 months after the bariatric surgery.

    Materials and Methods

    This cross‑sectional study was performed on 77 type 2 diabetes patients’ candidates for bariatric surgery (Roux‑en‑Y [RYGP] and Omega). Postoperative implementation protocol was one‑third of insulin for patients taking long‑term insulin and the discontinuation of medications for patients of oral antidiabetic agents. Blood glucose (BG) level was checked regularly by the patients at home and the necessary medical management was applied. The weight, BG and HbA1C levels, and use of oral antidiabetic agents and insulin were assessed and recorded before 1, 3, and 6 months after the surgery.

    Results

    BG levels and HbA1C percentage in the 1st, 3rd, and 6th months after the intervention in Omega group were significantly lower than RYGB group (P value < 0.05). At 1 and 3 months after surgery, the mean insulin dose received by the Omega and RYGB groups was reduced to <30 units/day and 10 units/day, respectively, following the management protocol in this study. Moreover, 23.1% and 7.7% of patients in RYGB group and 12.1% and 3% of patients in Omega group took oral antidiabetic agents 1and 3 months after surgery, respectively. Insulin and oral antidiabetic agents were completely discontinued 6 months after the surgery.

    Conclusion

    The long‑term management and support of the patients by the implementation of a standard protocol after surgery are of great significance in obtaining the optimal outcome after bariatric surgery.

    Keywords: Antidiabetic Agents, Bariatric Surgery, Insulin, Patient Care Management, obesity, Roux‑en‑Y Anastomosis, Type 2 Diabetes
  • Neda Tavakoli, Saeed Kaviani, Zahra Amini* Page 42
    Background

    The present study investigated the prevalence of illness anxiety disorder and its relationship with social health in the elderly population.

    Materials and Methods

    This cross‑sectional study was conducted on 400 elderly people in Isfahan referred to the educational health service in 2020. In addition to demographic characteristics, they answered the 36‑item Evans hypochondriasis questionnaire and the 28‑item social health questionnaire.

    Results

    Four hundred elderly participants with a mean age of 68.1 ± 6.6 (range: 60–89) were included in the study. One hundred and ninety‑nine (49.8%) were female and 201 (50.3%) were male. Only 24.3% of the geriatrics were in the healthy group and 21.2% in the borderline category. Seventy‑two of the geriatric population (18%) had poor social health and 299 of this population (74.8%) had moderate social health, and the social health status of 29 elderlies (7.3%) was favorable. There was an inverse relationship between hypochondriasis score and total social health score and its dimensions (P < 0.05).

    Conclusion

    High prevalence of hypochondriasis was observed in the geriatrics compared to global studies. Most of the elderly population had moderate social health. Gender and education do not affect hypochondriasis and social health in the elderly. People with higher social health scores were clearly less likely to get hypochondriasis. Therefore, by improving the various dimensions of social health of the geriatrics, we can help reduce the prevalence of hypochondriasis in this age group.

    Keywords: Elderly, hypochondriasis, anxiety disorder, social health
  • Omid Yaghini, Neda Hoseini*, Mohammad Reza Ghazavi, Vahid Mansouri, Jafar Nasiri, Toktam Moosavian, Mohammad Mehdi Salehi Page 43
    Background

    Migraine, one of the most common headaches in children, has a significant impact on children and their family’s quality of life (QoL). There are two approaches for controlling migraine headaches preventative and controlling acute attacks. Several drugs have been used for this purpose, and tricyclic antidepressants were at the top. Amitriptyline has shown not only a desirable effect on controlling the headaches but also some adverse side effects. Recently, finding effective drugs with fewer side effects, become more critical. Among them, nutraceuticals were one of the promising ones.

    Materials and Methods

    In this randomized clinical trial on 72 patients aged 5–15 years old with diagnosis of migraine based on the International Headache Society criteria, we compare the effectiveness of coenzyme Qten on frequency, duration, and severity of childhood migraine. For comparing the QoL, we used the International PedMIDAS questionnaire.

    Results

    Coenzyme Qten showed good therapeutic effects in children, especially in long‑term use; however, amitriptyline showed more rapid response. After 3 months of treatment, clinical outcomes in the two groups did not significantly differ from each other. Similarly, Children’s QoL increased in the same way. There are more reported side effects in children using amitriptyline compared to coenzyme Qten.

    Conclusions

    According to results, Co‑enzyme Q10, with fewer side effects and comparable therapeutic effects, especially in the long term, could be a good drug for prophylactic treatment of migraine headaches.

    Keywords: Child, clinical trial, co-enzyme Q10, migraine disorders, therapy amitriptyline
  • Arvin Barzanji, Karim Nasseri, Shahram Sadeghi, Mahsa Ardalan, Bijan Nouri, Khadijeh Daseh* Page 44
    Background

    Nausea and vomiting is a common complication after gynecological surgeries, especially laparoscopy, which can lead to discomfort and restlessness in the patients. The aim of the study was to compare the effect of ondansetron–dexamethasone and metoclopramide–dexamethasone on postoperative nausea and vomiting following gynecological laparoscopy.

    Materials and Methods

    In this double‑blind clinical trial, 68 females scheduled for gynecological laparoscopy and age range of 18–40 years were randomly divided into two groups. Group OD received ondansetron 4 mg plus dexamethasone 8 mg and group MD received metoclopramide 10 mg plus dexamethasone 8 mg, 15 min before the end of surgery. The incidence of nausea and vomiting and need for rescue medication was assessed during the recovery period, as well as at 2, 4, 6, 12, and 24 h after surgery. The data were analyzed using STATA software version 12 and a significance level of <0.05 was considered in this research.

    Results

    The incidence of nausea in ondansetron and metoclopramide groups was 23.3% and 33.3%, respectively, and the frequency of vomiting was 10% and 16.6%, respectively, which showed no significant difference (P > 0.05). The highest incidence of nausea and vomiting in patients belonged to the metoclopramide group inside 4–6 h after surgery.

    Conclusion

    Our study showed that no significant difference was observed in the incidence of nausea and vomiting between ondansetron–dexamethasone and metoclopramide–dexamethasone groups following laparoscopic gynecological surgery; however, the number of patients with nausea and vomiting was lower in the ondansetron–dexamethasone group

    Keywords: Laparoscopy, metoclopramide, ondansetron, postoperative nausea, vomiting
  • Srihari Dhandapani*, Sanjay Kambar, Murigendra Basayya Hiremath Page 45
    Background

    Diabetes mellitus patients are more likely to undergo nontraumatic foot or leg amputations because they have decreased circulation to the feet or reduced sensation. This condition may cause ulcerations, infections, and delayed wound healing, leading to amputations. Adequate knowledge and practices regarding foot care will be vital in the prevention of diabetic foot complications. Hence, this study aimed to determine the level of knowledge and practices of foot care among diabetes mellitus patients attending a rural primary health center in Belagavi‑South India.

    Materials and Methods

    A cross‑sectional study was conducted in the rural field practice area of J.N. Medical College, Belagavi, for 7 months (November 2019 to May 2020). A pretested questionnaire was administered to 461 diabetes patients attending the health center. A written informed consent was obtained from each participant. They were interviewed with a questionnaire consisting of 15 “yes” or “no” questions, each on foot care knowledge and practice.

    Results

    The mean age of the study participants was 50.28 ± 9.48 years. The mean duration of diabetes was 10.69 ± 7.09 years. Majority, i.e., 296 (64.2%) participants showed satisfactory knowledge and 293 (63.6%) showed satisfactory practice. Only 97 (21%) had poor knowledge, 90 (19.5%) had poor practice, 68 (14.8%) patients had good knowledge, and 78 (16.9%) had good practice about diabetic foot care.

    Conclusions

    The low level of knowledge and practice in patients with diabetes regarding foot care necessitates an educational program to increase awareness related to foot complications of diabetes, thereby motivating diabetic patients to incorporate foot care practices in their day‑to‑day routine.

    Keywords: Complications, diabetes mellitus, diabetic foot, epidemiology, foot care, India, knowledge, practice, prevention, control, primary health center