فصلنامه گیاه و زیست فناوری ایران
سال هفدهم شماره 1 (بهار 1401)

To investigate the effect of soil application and foliar application of iron on growth indices and its ability to be absorbed by Mirabilis jalapa, an experiment was conducted in a completely randomized design with three replications in greenhouses located in the Golgohar iron ore mine in Kerman. Different levels of iron chelate used in this experiment included soil application (zero, 15, 30 and 90 mg kg-1 soil) and foliar application (2, 4 and 6 mg l-1 ). Soil application of iron chelate was initially tested with potting soil and foliar application was applied at the stage of stem and flowering. The results of this study showed that iron had a significant effect on the evaluated traits, so that the highest fresh and dry weight of shoots and roots, plant height, root length, chlorophyll a, b and total in soil iron treatment of 90 mg kg-1 it was observed that also the highest uptake of iron in shoots and roots was obtained in soil application of 90 mg kg-1 soil. In general, the results showed that the application of iron increased the growth indices and photosynthetic pigments of Mirabilis jalapa. Also, soil application of iron (90 mg kg-1 soil) had a better result than foliar application, on the other hand due to its ability to absorb iron by aerial parts and roots of this plant can also be used as a purifier of iron-contaminated soils.

This study was designed and conducted to investigate the effects of foliar application of Iron, Potassium, Calcium and Manganese nanoparticles on the amount of elements and enzymatic activity of peppermint (Mentha piperita) in a completely randomized design in experiments. Plants were sprayed with three levels of Iron, Potassium, Calcium and Manganese nanoclates (2, 4 and 6 ppm) and the experiment consisted of 13 treatments, each with 3 replicates, each replicate containing 3 plants and a total of 117 pots were examined. In this study, 4 to 6 leaflets peppermint transplants were cultured in appropriate media (peatmoss, sand and clay at a ratio of 1: 1: 1). Foliar application was performed three times a week and after two weeks, sampling and evaluation of traits including superoxide dismutase and peroxidase activity, Iron, Ppotassium, Calcium and Manganese content and essential oil percentage were determined. The results showed that in experiment, the effect of treatment on Iron content had significant difference at 5% and other traits at 1%. Also the highest level were observed in traits such as superoxide dismutase and peroxidase enzymes activity and leaf Iron content in iron nanoclates 6ppm treatment, maximum Potassium content in potassium nanoclates 6ppm treatment, highest The amount of Manganese in manganese nanoclates 6ppm treatment, the highest Calcium leaf content in calcium nanoclates 6ppm treatment and the highest essential oil percentage in iron nanoclates 4ppm treatment. The lowest of all traits evaluated was in control treatment.

The Digitalis nevrosa has anti-cancer properties due to its glycosidic compounds The aim of this study was to investigate the anticancer effects of hydroalcoholic extract of digitalis nevrosa on MCF-7 breast cancer cell line. In this experimental study, hydroalcoholic extract of thistle was produced. MCF-7 cells were then treated with different concentrations (3.125, 6.25, 12.5, 25, 50 and 100 μg / ml). The effects of cytotoxicity of the extract were analyzed by MTT method and after determining the concentration of 50% lethality of the extract, the expression changes of P53, Bax, Bcl2 and CDH1 genes were analyzed by real-time PCR. The data were evaluated using SPSS software and one-way ANOVA and Tukey test. The results of MTT showed that the effect of the extract on viability was dosedependent and the highest cytotoxicity was related to the concentration of the extract at 39/45μg / ml. Which were statistically significant were shown to be significant.(P<0.001). Also, the effect of silver nanoparticles with a concentration of 50% lethality of 39/45 μg / ml on the expression of P53, Bax, Bcl2 and CDH1 genes in comparison with the control gene showed significant changes. Digitalis nevrosa extract induces apoptosis in MCF-7 cell line and the use of this plant can be considered as a promising choice in the treatment of breast cancer.

It is very important to increase the life of flowers in cut flowers, which are perishable products and have a short life. By using different compounds and materials, the lifespan of cut flowers can be increased. The factorial experiment was conducted in a completely randomized design with four replications including 64 pots in Mashhad. Peppermint essential oil was the first factor in four levels (0, 100, 200 and 300 mg/l) and citric acid was the second factor in four levels (0, 100, 200 and 300 mg/l). To evaluate the shelf life of cut flowers based on the day, the time interval from harvest to when the number of wilted florets were more than open florets was considered as the end of flowering life. The results showed that peppermint essential oil on flower life was significant at 5% probability level and the effect of citric acid on number of opened florets, florence diameter, florence diameter, wilting percentage, stem fresh weight and flower longevity at 1% probability level Significant as well as the interaction of experimental treatments on the number of open florets, florence shedding and floreter diameter at 5% probability level and the percentage of wilting percentage, stem fresh weight and longevity at 1% probability level were significant. The highest number of open florets (4.62), floreter diameter (21.71 mm), fresh weight (75.82 g), flower longevity (7.88 days) related to 300 mg/l treatment Peppermint essential oil was 200 mg / l citric acid. In general, the use of peppermint essential oil and citric acid in different concentrations compared to the control increased flower life, fresh weight, number of open florets and diameter of florets as well as reduced florence and wilting percentage of sage.

Metal nanoparticles synthesized using medicinal plants are used in anti-cancer and antimicrobial studies due to their unique physicochemical properties, high safety and low cost.

Silver nanoparticles were synthesized using extracts of hyssop and marjoram. After studying the properties of the synthesized nanoparticles, two gram-negative Escherichia coli and gram-positive Staphylococcus aureus bacteria were used to evaluate the antimicrobial effect of the synthesized nanoparticles. Finally, the anti-cancer effect of synthesized silver nanoparticles on 3 cancer cell lines was evaluated by MTT method.

The results showed that the synthesized nanoparticles were spherical in shape and their average size was between 15 and 26 nm. X-ray diffraction test also confirmed the successful synthesis of nanoparticles. The results of antimicrobial test showed that the synthesized silver nanoparticles had a significant antimicrobial effect on both bacteria. The anti-cancer effect of the synthesized nanoparticles was similar on all 3 cell lines. However, with increasing the concentration of nanoparticles on the survival of cancer cells decreased, indicating a direct dose interaction on the inhibitory rate of silver nanoparticles. Discussion and

Medicinal plants can be used in the synthesis of silver nanoparticles. The synthesized nanoparticles, due to their coating of secondary metabolites and the release of silver ions (Ag+ ), can be used as effective therapeutic agents in the treatment of various cancers

The type of land use, the amount of vegetation and their management, play an important role in the carbon sequestration capacity of the ecosystem. Therefore, in order to estimate soil carbon sequestration over time affected by vegetation type, in Gonabad city, first using remote sensing data of ETM and TM satellite images, land use map of the city for the years 1387 and 1397 based on the maximum probability classification method. Similarity was provided. Vegetation index was also calculated for the images. In order to estimate the amount of soil carbon sequestration in the two years studied and to investigate the relationship between depth, texture and percentage of soil nitrogen with the amount of carbon sequestration in pistachio and wheat, soil test results and GPS points of these uses were prepared using independent t-test And regression relations data analysis was performed. The results showed that different land uses with different vegetation levels have different effects on soil carbon sequestration. So that the total amount of soil carbon sequestration in pistachio orchards in 1397; It had the highest rate of carbon sequestration per hectare. The study of the relationship between depth, texture and percentage of soil nitrogen with carbon sequestration showed that there is a positive relationship between the percentage of nitrogen, silt and soil clay and a negative correlation between sand and carbon sequestration.