مجله دانشگاه علوم پزشکی سبزوار
سال بیست و نهم شماره 3 (پیاپی 122، امرداد و شهریور 1401)

Changes in the SET1B gene expression, can directly affect the incidence and progression of cancer. The gene encoding lncRNA LIMT is transcribed as antisense in the opposite direction to SET1B.  The aim of this study was to investigate the expression of lncRNA LIMT and SETD1B in tumor tissues compared to adjacent normal in colorectal cancer and the relation between these two genes is related to the clinical features of tumor tissues.

After collecting 40 tumor and adjacent normal tissues, Total RNA extraction and cDNA synthesis were performed. Then the expression levels of the desired genes in tumor and normal tissues was compared. Finally, the obtained results were statistically analyzed by Prism software.

The expression level of SETD1B increased 1.8 fold changes in tumor samples (p = 0.01103) while the expression level of lncRNA LIMT in tumor tissue did not change significantly compared to normal tissue (p = 0.5391). In addition, the expression levels of SET1B and lncRNA LIMT in the two age groups over 60 years and under 60 years in tumor tissues did not change significantly. ROC analysis also showed that SETD1B with AUC = 0.336 and CI = 0.8771 - 0.9902 can separate the patient population from the healthy and can help diagnose colorectal cancer.

According to the results of this study, it can be said that SETD1B is increased in tumor tissue and can be used as a biomarker for colorectal cancer.

In recent years, researchers have considered the anticancer activity coumarins, due to their powerful biological activity and poor toxicity that can neutralize the side effects induced by radiotherapy. The aim of this study was to investigate the cytotoxic effects of coumarin on HT-29 and A549 cancer cells.

In this experimental study, the stoke of coumarin was prepared and then for 1,3, and 5 days at concentrations of5, 10, 15, 20, and 25 μM the cells were treated and evaluated on viability days and morphology of the cells indefinite days. The IC50 concentration of coumarin was calculated using MTT assay in two cell lines. Also, the expression of the involved genes in apoptosis such as Bax, Bad, and Bcl-2 was evaluated by the qRT_PCR method. Data were analyzed by a one-way ANOVA test.

The results showed that coumarin reduced the viability and proliferation of HT-29 and special A549 cells by dose and time significantly (P≤0.001), as well as the viability rate of cells in treated cells on the fifth day, significantly decreased compared to the control group (P <0.05). Morphological changes such as reduced chromatin density, cell turnover were also noticeably observed in the cells. Also, molecular results showed that coumarin could significantly increase the expression of Bax, Bad genes and decrease the expression of Bcl-2 gene expression. That these genetic changes in A549 cells were significantly greater than HT-29.

Coumarin is capable of anti-proliferative activity and induces apoptosis effectively against colon and lung cancer cells.

Women's health is the basis of family and community health. Previous studies showed that one of the factors that leads to the promotion of women's general health is the level of different cognitive and emotional skills. The aim of this study was to compare the acceptance and commitment based therapy and cognitive rehabilitation of working memory and their integration on depressed girls' general health.

The research method was quasi-experimental with pre-test, post-test and follow-up stages with a control group. The statistical population of this study included all girls referred to psychological centers in Sabzevar in 2019 that of these clients 60 people were randomly selected and divided into 3 experimental groups and 1 control group. Participants in the first intervention group underwent 8 sessions of acceptance and commitment therapy, the second one underwent 10 sessions of cognitive rehabilitation of working memory and the third group underwent 8 sessions of acceptance and commitment therapy and 10 sessions of cognitive rehabilitation of working memory.

The results of the present study showed that there was a significant difference (p=0/001) between the effectiveness of acceptance and commitment therapy, cognitive rehabilitation of working memory and the combination of these two methods.

The results of the present study showed that the combination of two methods was more effective than acceptance and commitment therapy and cognitive rehabilitation by itself.

Different beliefs and attitudes of individuals sometimes lead to different forms of stigma and discrimination against a group with different beliefs. The aim of this study was to determine the degree of stigma and discrimination and to express the discriminatory practices of nursing students of Urmia Islamic Azad University in the face of HIV / AIDS patients.

In this descriptive cross-sectional study, 90 nursing students of 5th and 6th semesters of Islamic Azad University of Urmia were selected by census and examined using a questionnaire of behavior and professional care of patients with AIDS. SPSS 16 software and descriptive statistical tests were used to analyze the data.

76.7% believed that they could refuse to provide services to AIDS patients in order to maintain their health and that of their families. 55.5% of students considered fear of infection as the most important concern in the care treatment of AIDS patients. 7.8% of students stated that they refused to provide care treatment to an AIDS patient.

Increasing the stigma and discrimination of nursing students towards AIDS patients can be due to fear of infection and lack of preventive measures.

The COVID-19 pandemic has created the most health challenge in the recent years. Understanding the symptoms is important in the recognition of severity of disease and identification of its palliative factors. Spiritual health is known an important factor in reducing distress of patients, this study was designed to determine the relationship between spiritual health and symptoms of COVID-19 patients during the pandemic.

Data were collected using a cross-sectional design to select COVID-19 patients of a special hospital in Qom. Convenience sampling was carried out during the May– June 2020. Participants were asked to fill out the demographic questionnaire. The spiritual health and symptoms of patients were measured using the Paloutzain and Ellison Spiritual Health and the Edmonton Symptom Assessment Scales respectively. Pearson’s correlation coefficient, t-test and ANOVA was conducted to data analysis.

The findings demonstrated that most of the participants had high-average levels of spiritual health 70.14 (2.96) and average levels of symptoms 60.79(21.07). Spiritual health were negatively correlated with lack of Well-being (r= -.131, p < .05) and Anxious symptoms (r=-.115, p < .05). Religious health was correlated with Anxious symptoms (r= -.109, p < .05) and Drowsy (r= -.114, p < .05) and lack of Well-being (r= -.115, p < .05).

This research confirmed the relationship of Spiritual health with some symptoms. Single patients and older adults had higher levels of symptoms and their management is a priority during the pandemic.

The cost of hospital services has increased sharply, so we must pay attention to the proper management of costs in hospitals and the need to make optimal use of available facilities and resources by using economic analysis. This study aimed to investigate the types of efficiency of hospitals in Sabzevar University of Medical Sciences before and after establishing the Health System Reform.

This descriptive-analytical study was conducted to measure hospitals' technical, scale, and managerial efficiency under the auspices of Sabzevar University of Medical Sciences using the comprehensive data analysis model and Pabon Lasso model in 2014 and 2018.

Hospitals' average technical efficiency and scale had increased from 0.945 in 2014 to 0.924 in 2018. Also, the average change in total productivity was equal to 0.978, which indicates an improvement in the productivity situation during the study period.

One of the reasons for the increase in the number of hospitals with technical efficiency and scale 1 is the start of Heshmatieh Hospital and the optimal use of resources by the above hospital. In this study, the Malmquist index and Pabon Lasso model show that hospitals' performance has improved after establishing the Health Reform Plan. Still, the values of various types of performance calculated through data envelopment analysis show a slight decrease in performance. Sabzevar hospitals are after the implementation of the Reform plan. Therefore, the correct distribution of resources effectively improves the hospital's function.

Preeclampsia and eclampsia are pregnancy specific syndromes. Vitamin D is one of the pathological factors of this syndrome. The aim of this study was to compare the serum levels of vitamin D in pregnant women with preeclampsia or eclampsia with healthy pregnant women.

In a cross-sectional, descriptive and analytical study, 50 pregnant women with preeclampsia or eclampsia and 50 healthy individuals were selected based on inclusion and exclusion criteria, and after taking blood sampling, serum vitamin D levels were measured using a commercial kit and ELISA. Statistical analysis was done by SPSS: 20. P <0.05 was considered as significant difference.

The mean serum vitamin D in pregnant women with preeclampsia or eclampsia was lower than the healthy group (P = 0.002). Also, 38% and 2% of pregnant women with preeclampsia or eclampsia and healthy had severe vitamin D deficiency. Vitamin D deficiency was more common in pregnant women over 30 years of age and BMI over 25, education less than a diploma, a housewife, and a gestational age of less than 34 weeks.

The results indicate that most pregnant women with preeclampsia and eclampsia syndrome are deficient in vitamin D, which affect in the development of preeclampsia or eclampsia. Therefore, timely screening and treatment of vitamin D deficiency can probably prevent preeclampsia or eclampsia and promote the quality of pregnancy.

Inadequate nutrition is one of the causes of high cholesterol and blood lipid, which if untreated, due to the high inflammation of liver cells, can lead to fatty liver. The aim of this study was to evaluate the anti-hyperlipidemia effect of herbal tea of Rosa damascena Mill and Cassia angustifolia Vahl on lipid profile in male rats.

In this experimental study, 28 male rats (220 ± 20 g) were randomly divided into four groups (n = 7 each): control, control + high-fat diet, experimental group + high-fat diet + herbal tea, and experimental group + herbal tea. The groups were treated with herbal tea of Cassia angustifolia Vahl (0.4 g/kg) plus Rosa damascena Mill (0.2 g/kg) in three time periods with two days interval. After 28 days, the animals were anesthetized, and blood samples were collected from the heart. Blood parameters were measured using an autoanalyzer.

Based on the results, lipid profile, except for the AST enzyme, significantly decreased in the experimental group with high-fat diet + herbal tea. In the experimental group with normal diet + herbal tea, the profile did not show any significant difference; however, AST enzyme increased significantly (p ≤ 0.05), as compared to the control group.

Herbal tea prepared with a combination of Cassia angustifolia Vahl and Rosa damascena Mill can be considered as a drug with rapid efficacy in people with hyperlipidemia.

At present, Pseudomonas aeruginosa bacteria has caused many problems to control nosocomial infections due to its resistance to various antibiotics and MexAB-OprM efflux pumps play an important role in making Pseudomonas aeruginosa strains resistant to antibiotics. The aim of this study was to determine the antimicrobial effect of silver nanoparticles and probiotic Lactobacillus plantarum on MexA gene expression.

In this descriptive cross-sectional study, 49 strains were collected from 8 medical diagnostic laboratories in Mashhad during the years 1398-1399 for 8 months. Evaluation of antibiotic susceptibility pattern of strains was using disk diffusion method based on CLSI protocol. After treatment of multidrug resistant bacteria with inhibitors, microdilution method and Real time-PCR technique were used to determine the effective dilution of silver nanoparticles and probiotics on the expression of MexA gene of its producing bacterium, respectively.

All samples were identified and all of them were resistant to more than two antibiotics and had MexA gene. The minimum growth inhibition concentration in agar dilution method was up to 250 μg / ml for silver nanoparticles and up to 18 μg / ml for probiotics. Silver nanoparticles had a greater effect in inhibiting bacterial growth compared to probiotics and this effect is less than the combined effect of probiotics and silver nanoparticles (P> 0.05).

Silver and probiotic nanoparticles have inhibitory and antibacterial activity to reduce the performance of MexAB-OprM efflux pump in Pseudomonas aeruginosa. (P> 0.05).

Many psychological and emotional processes can be involved in the vulnerability to irritable bowel syndrome (IBS). This study aimed to explain the causal relationship between emotional schemas and the severity of symptoms in patients with irritable bowel syndrome based on the mediatory role of anger.

The descriptive-correlational method was used in this study for data collection. The statistical population consisted of all patients with irritable bowel syndrome referred to Imam Reza Hospital's Gastrointestinal Clinic in Mashhad in 2020, from whom 323 individuals were selected by convenience sampling. Research tools included Irritable Bowel Syndrome Severity Scoring System (IBS-SSS) by Francis et al. (1997), Novaco Anger Scale (NAS) (1994), and Leahy Emotional Schema Scale (LESS) (2002). The data were analyzed by structural equation modeling.

The indirect pathway coefficient between non-adaptive emotional schemas and the severity of irritable bowel syndrome symptoms (p<0.01, β=0.128) was positive and significant at the level of 0.01. Also, the indirect path coefficient between adaptive emotional schemas and severity of irritable bowel syndrome symptoms (p<0.01, β=-0.139) was negative and significant at the level of 0.01.

The present study results show that anger mediates the relationship between adaptive and non-adaptive emotional schemas with the severity of irritable bowel syndrome symptoms.

Considering the importance of recognizing individual changes in old age and the special importance of retirement satisfaction, this study was conducted to determine the relationship between cognitive ability of retirees from individual change and retirement satisfaction in great Torshiz.

This cross-sectional descriptive-analytical study was conducted in 1399 on 330 randomly selected retirees in Kashmar city. Data were collected using demographic, retirement satisfaction and cognitive ability of individual changes questionnaires. Data were analyzed by descriptive statistics with SPSS software version 16. 

The mean age of participants was 62, 05+4,13. Retirement satisfaction was 20.27±76.29. Retirement satisfaction was significantly associated with marital status, age, income, type of property, study, entertainment, sports, television watching (P <0.05). The mean cognitive ability of retirees was 5.60±36.14, that the degree of cognitive ability was related to factors such as the number of hours spent watching TV and the type of illness involved (P <0.05). Statistical test did not show a significant relationship between retirement satisfaction and cognitive ability (P = 0.16).

Because most of the retirees had poor cognitive ability, it seems necessary to improve the cognitive ability of retirees in the last years of their careers. There was no relationship between cognitive ability and retirement satisfaction, which may be due to the region under study. Therefore, it is recommended to re-study in another environment to evaluate this relationship.