مجله به زراعی کشاورزی
سال بیست و چهارم شماره 3 (پاییز 1401)

In order to investigate the effect of tillage systems and the combination of chemical fertilizer and livestock manure on some characteristics of sunflower, an experiment has been conducted as split plots in a randomized complete block design with three replications at the research farm of University of Zabol during 2019. The main factor has been tillage levels (1. moldboard plow and disc, 2. sweep and disc, and 3. disc). The sub-factor include fertilizer levels (no fertilizer application, 100 kg/ha of diammonium phosphate, 25 tons/ha of livestock manure+ 100 kg/ha of diamonium phosphate, 50 tons/ha of livestock manure + 100 kg/ha of diamonium phosphate, 25 tons/ha of livestock manure, and 50 tons/ha of livestock manure). Results show that the highest values belong to the 50 tons/ha of livestock manure in tillage with disk for grain yield (5072 kg/ha), and oil percentage (44.44%), 50 tons/ha of livestock manure in tillage with disc for oleic acid content (51.61%), and 50 tons/ha of livestock manure for linoleic acid content (31.1%). The highest amount of chlorophyll a, b and carotenoids (1.253, 0.45, and 1.04 mg/g, respectively) has been obtained from the application of 100 kg/ha of diamonium phosphate in tillage with disc. In general, in order to increase and improve the quantitative and qualitative characteristics of sunflower, the application of 50 tons/ha of livestock manure is suitable in disc tillage conditions.

In order to investigate the effect of different weed control managements on emergence characteristics and morphological growth of rice seedlings in the seedling stage in a direct seeding system, a field experiment has been conducted in the 2020 growing season, Babolsar, Bahnemir, Iran. Being a factorial in a randomized complete block design with three replications, the experiment employs the following treatments: Shiroudi, Khazar, and Hashemi cultivars as well as various weed control management in five levels of seed coating with calcium chloride, seed coating with potassium chloride, weeding, chemical control, and control. The interaction effect of weed management and cultivar shows that coating the seeds with calcium chloride, potassium chloride, and weeding cuts the dry weight of grasses by 55%, 68%, and 85% in Shiroudi cultivar, by 79%, 63%, and 54% in Khazar cultivar, and 30%, 58%, and 35% in Hashemi cultivar, respectively, compared to the control. The maximum percentage of germination with 99%, 97%, and 95% belong to seed coating with potassium chloride, calcium chloride, and weeding in Shiroudi cultivar, respectively. The highest germination rate could be observed in potassium and calcium chloride seed coating treatments and weeding. The lowest density of broadleaf weeds with 0.91 plants m-2 is obtained in Shiroudi cultivar and by managing the seeds coating of this cultivar with calcium chloride. The highest seedling length and weight vigor indices are obtained with 3559 and 137 in Shiroudi cultivar under weeding management, respectively. Results show that seed coating improves the seed yield of rice cultivars.

The field experiment has been conducted as a split split plot based on a randomized complete block design with three replicaitons at Rice Research Institute of Mazandaran Province, Amol, in 2017. In this experiment, the seeds are sown in seedling box with different six bed treatments. These beds include S1: sulfur bed with 50% rice bran, S2: sulfur-free soil with 25% rice bran, S3: sulfur bed with 25% rice bran, S4: sulfur bed with Thiobacillus and 50% rice bran, S5: sulfur-free bed with wood compost and 25% rice bran, and S6: sulfur bed with Thiobacillus with 25% bran rice. After some measurements, the seedling is transplanted in the main field. In the field, different beds in box, seedling number per hill (3 and 5) and planting space (20×20 cm, 30×14cm) are assigned to the main plot, sub plot, and sub sub plot, respectively. The results of main cultivation show that the highest grain yield (5043 kg.ha-1) and biological yield (12319 kg.ha-1) are obtained from the treatment of bed soil with sulfur with 50% bran. Planting arrangement treatment in ratoon cultivation on number of hollow seeds (4.1), grain yield (1617 kg.ha-1), number of plants per hill treatment on number of tillers (10.3), leaf area (10.6 cm2), panicle length (20.4 cm) and grain yield (1613 kg.ha-1), and seedling type treatment on tiller number (10.4) show a statistically significant difference. Seedling grown in sulfur with 50% rice brain display the best performance in terms of yield and yield components in both main and ratoon cropping.

To investigate the effect of different levels of irrigation and nitrogen fertilizer on yield and yield components of some safflower cultivars, an experiment was conducted as a split-split plot in a randomized complete blocks design with three replications in the research farms of the University of Tehran, Karaj during 2018-2019. Main plots were irrigation (normal irrigation, irrigation until at middle of flowering stage and irrigation until the beginning of seed filling), sub plots were nitrogen (supply of 100 % and 50 % of nitrogen needs) and sub-sub plots were safflower cultivars (Goldasht, Golmehr, Padideh and Parnian). The effect of irrigation, nitrogen, and cultivar factors on number of heads per plant, number of seeds per head, 1000-seed weight, grain yield, and biomass were significant. According to the results, cut-off of irrigation at the beginning of seed filling and middle of flowering stages leads to a reduction of 16.2 % and 68.1 % of seed yield compared to normal irrigation, respectively. Reducing the amount of nitrogen by 50 % compared to 100 % supply decreases seed yield 8.5 % and biomass by 12 %. The triple interaction of irrigation, fertilizer and cultivar was significant in stem diameter and head diameter. The interaction of irrigation and nitrogen levels showed that with decreasing irrigation levels, nitrogen uptake also decreased and reduced the number of seed per head. However, providing 100 % of the nitrogen requirement under drought stress conditions prevents a more severe decline in the number of grains per head.

In order to study the effect of cytokinin, gibberellin, and cycocle on yield and yield components of two wheat cultivars under different irrigation regimes, a study was conducted as a split-split experiment based on a randomized complete block design with three replications at the Agricultural Research Station of Kurdistan University in 2016. There, the irrigation factor at four levels: dryland, irrigation at booting stage, irrigation at booting + flowering and irrigation at booting + flowering + grain filling stages have served as the main plots, two wheat cultivars (Sirvan and Homa) as sub-plots, and levels of plant growth regulators foliar application including, i.e. control, gibberellin (100 µm), cytokinin (100 µm), and cycocel (3gr. lit-1) at the wheat stem elongation and grain filling stages as the sub-sub plots. Results show that in the foliar application during the stem stage, compared to the dryland treatment, yield increase in one, two, and three irrigation treatments have been 29%, 33%, and 43%, and at the grain filling stage; 22%, 28%, and 33%, respectively. Homa cultivar produced higher yield compared to Sirvan. In the foliar application during the stem stage, the highest grain yield (504 g m-2) belongs to application of three irrigation treatments as well as addition of cytokinin, and at the grain filling stage, the highest grain yield (477.6 g m-2) has been obtained from Homa cultivar under three times irrigation condition and cycocel consumption. Therefore, using even one stage irrigation and the application of cytokinin and cycocel can increase the grain yield of wheat.

To identify the limiting factors in forage maize production in Iran, this study has been carried out in order to separate the effective and influential management factors in forage maize production in the country with data. It is obtained from 43 final reports or their derived extracted articles in Agricultural Research, Education and Extension Organization (AREEO) with meta-analysis method. According to the results, plant density (5.65%), drought stress (-13.44%), cultivar (0.31%), planting date (2.54%), and nitrogen fertilizer (24%) justify the amount of forage maize variation. Based on the results of this study, forage yield has increased by about 9.49%, increasing plant density between 80 to 100 thousand plants per hectare. Maize forage yield has decreased significantly under mild stress, severe stress, and very severe drought stress by 25.30%, 14.38% and 8.99%, respectively. Also, for different cultivars and different groups of maturity, group-700 has had a significant increase of 3.83% compared to other groups. For the planting dates of forage maize compared to the control, which is the first half of June, a decrease in forage yield is observed. Finally, the highest percentage of increase in maize forage yield has been obtained from the use of 450 kg ha-1 of urea fertilizer. Overall, nitrogen fertilizer, drought stress, and planting density are recognized as the most important factors affecting corn forage yield in Iran, respectively.

In order to evaluate the planting date delay on phenological traits, yield components, grain yield, and linoleic and linolenic fatty acids of new sunflower hybrids, an experiment has been conducted as split plot in a randomized, completely block design, with four replicates at the field of oilseed section of seed and plant Improvement during 2014-2016 growing season. The treatments include seven sunflower hybrids (Barzegar, Farrokh, Haysan 36, Qasem, Shams, Azargol, and Haysan 25) on three planting dates (15 days after harvest of autumn (wheat), July 1, July 15, and July 30, respectively). The experimental results show significant effects of planting date on all traits except number of seeds per head and linolenic acid at the level of 1% and on the percentage of oil and linoleic acid at the level of 5%. The studied hybrids are significantly different in all traits except linoleic and linolenic fatty acids. The latest experimental hybrids include Barzegar and Azargol hybrids and the earliest hybrid is Farrokh. The highest grain yield is obtained from the date of the first sowing at the rate of 2840 kg / ha and in the case of delayed sowing the yield is reduced to 41%.

This experiment has been performed to study the effect of seed sowing density and different amounts of nitrogen fertilizer application on the remobilization indices in wheat (Qaboos cultivar) in the field of Gonbad Kavous Agricultural and Natural Resources Research Station in two years (2018-19 and 2019-20) in three replications and as a split-plot in a randomized complete block design. The main plots include pure nitrogen at four levels, i.e., 0 (control), 46, 92, and 138 kg/ha of nitrogen from urea fertilizer source), and the subplots are planting density at six levels (150, 225, 300, 375, 450, and 525 seed/m2). The remobilization photosynthetic assimilate separately from leaves, main stem (without leaves), and seedless spike components are examined to study the process of photosynthetic material transfer to seed. Results show that there has been significant differences among planting density, nitrogen fertilizer, and interaction of planting density× fertilizer treatments in terms of grain nitrogen percentage and remobilization traits of wheat. The highest percentage of grain nitrogen (1.87%) has been obtained in the treatment of 138 kg/ha nitrogen and 450 seed/m2. In the first year, the highest remobilization from the plant is observed in the treatment of 92 kg/ha nitrogen and 375 seeds/m2 (0.528 gr/plant), and in the second year, in the control, the highest remobilization is observed in the planting density of 300 seeds/m2 (0.345 gr/plant), and then with increasing planting density, the remobilization from the plant is reduced.

The use of densitiy and cultivars that have high competitiveness are effective ways to control weeds in the integrated management system. The present study aims at increasing competitive ability of some dryland chickpea cultivars against weeds under different plant densities. Therefore, an experiment has been conducted as a split factorial in a Randomized Complete Block Design with four replications in the research farms of the Kurdistan Agricultural and Natural Resources Research and Education Center, Garizeh Agricultural Research Station, Sanandaj, Iran, during the 2020-2021 growing seasons. Experimental treatments include different plant density (30, 36, and 42 plants.m-2) as main plot, seven cultivars (Azad, Jam, Hashem, ILC482, Pirooz, Kaka, and a local variety), and weed management (no weeding and hand weeding during the whole growing season) as subplots. Results indicate that weeding operations are effective in increasing the number of pod per plant by 35.63%. Also, it is observed that there have beem significant differences among yield and yield components of the studied cultivars. ILC482 and Kaka cultivars have had the highest and lowest number of main branches at 3.82 and 2.58 values, respectively. The highest number of secondary branches obtained at a density of 30 plants.m-2. Furthermore, the highest number of pods per plant registered for Pirooz and Jam cultivars and the lowest number of pods per plant has been 7.98 in Hashem cultivar. The highest grain yield, indices of tolerance and competition and the lowest weed density observed for the Jam cultivar. In all examined cultivars, the number of seeds per square meter, grain yield and ability withstand competition index improve with increasing plant density so that the highest values are achieved at the plant density of 42 plants.m-2.

Genetic resources of Iranian rye have a high adaptation and diversity which provides the required forage in diverse climates of our country. Utilization of these resources is important in the development of new varieties. In this study, nine rye ecotypes, along with commercial cultivars of rye, barley, and triticale have been evaluated in three growing seasons 2014-15, 2016-17 and 2017-18 in research field of Seed and Plant Improvement Institute. The research is carried out in three separate experiments in a randomized complete block design with three replications. The study of phenological, morphological, and agronomic traits show that interaction of year and ecotype is significant for all traits, except for days to flowering. Based on the dry forage yield, Ecotype 119 has had the highest mean and Triticale cultivar, the lowest (5.68 and 4.14 t/ha respectively). Correlation coefficeint between grain yield, forage yield, 1000 kernel weight, and flag leaf area is significant. Comparison of ADF percentage in the forage show that these traits are significantly lower in ecotype 119 and barley cultivar Valfajr than other samples (60% and 56%, respectively). Based on the average of fiber in forage, Ecotype 119 has had the lowest average (33%). It seems that Ecotype 119, in addition to high potential for forage production, also has good forage quality.

This study attempts to investigate the effect of application of humic acid and seaweed extract on morphology, growth, yield, and yield components of quinoa under drought stress in 2018-2019 in two different locations, Qasr Shirin and Dalahou. It has been performed as split plots in a randomized complete block design with 4 replications. Experimental treatments include three irrigation treatments (full irrigation, irrigation cut-off at the beginning of flowering and irrigation cut-off at the beginning of seed filling) and foliar spraying treatments with different amounts of humic acid (foliar application at the rate of 1.5 kg / ha and 2 Kg / ha) and seaweed extract (foliar application at 1 kg / ha and 1.5 kg / ha) along with a control treatment. Results show that seed yield in Dalahou region under normal condation has been 24.78% higher than Qasrshirin region. Seed yield in different irrigation treatments under the influence of foliar application show that in all irrigation treatments, the effect of foliar application of humic acid and seaweed extract on seed yield has been positive compared to the control. In full irrigation conditions, the highest seed yield is obtained from foliar application of humic acid, while in irrigation cut-off treatments, the effect of seaweed extract on seed yield has been better than humic acid. The weight of 1000 seeds in quinoa produced in Dalahu region has been more than Qasrshirin region but in both regions drought stress has reduced the 1000 seeds weight. In all irrigation treatments, application of humic acid and algae extract has increased 1000-seed weight.

A study was conducted as split plot layout with three replications at the research greenhouse of Bu-Ali Sina University, Hamedan in 2017 in order to investigate the consequences of using biochar and hydrochar, produced from potato plant residue on bean plant growth indices, chlorophyll content, and root mycorrhizal symbiosis in drought stress. The main plot and subplots in this study have been two drought levels and four amendment treatments, respectively. The study shows that the drought stress and the application amendments in various forms have had significant effects on plant growth indices, chlorophyll content, and root mycorrhizal colonization. Drought stress reduces root and shoot dry weights up to 39.8, 46.1%, leaf chlorophyll a (Chl a) content up to 52.6%, chlorophyll b (Chl b) content up to 58%, and total chlorophyll up to 54.52%. Although the number of rhizobium nodule on the root of plant decreases in drought stress, the rate of root mycorrhiza rises by 19.2% in drought stress. The use of biochar increased the mycorrhizal symbiotic indices significantly. It increases root colonization 11.34% and Glomeromycota spore number 50.5% in soil. The application of raw residue in soil has had the most positive effects on the plant growth indices and the leaf chlorophyll contents, leading to increased shoot dry weights (49.8%) and chlorophyll a, b and total contents (3.54%, 36.8%, and 14.5% respectively). The findings of this study show that among the treatments, the best plant growth index has been obtained in the use of potato biochar, which reduces the harmful effects of drought stress on the bean plant.

In order to investigate the effect of some biotic and abiotic elicitors on yield and yield components of stevia plant under different salinity stress, the present study has been perfoemed as a factorial experiment based on the randomized complete design with six replications. It has taken place in a greenhouse under hydroponic and conditions in two consequential experiments. Endophytic fungi isolated from yew (at three levels of control, TB20, TB2-3), foliar treatment with melatonin (at three levels of control, 0.5 μM melatonin tablets, and 0.5 μM Thymus vulgaris extract), and three salinity levels (without salinity, medium salinity 80mM, and high salinity 150mM) have incorporated the present experiments’ treatments. The experimental plants have been investigated based on morphological parameters. In both experiments, results show a positive effect of melatonin and endophytic fungi on characteristics of plant growth’s improvement (one to two times) under salinity stress. Nevertheless, the best growth conditions have been observed in low salinity levels, using thyme extract and TB20 endophytic fungus. Most traits of stevia plant in non-saline conditions along with thyme extract and endophytic fungus TB20 have been at their highest, showing an increase of approximately 1.5 folds, compared to the control. Due to the positive effects of melatonin and endophytes, especially TB20, it is recommended to use melatonin-containing plant extracts as a protective treatment in stevia cultivation in saline conditions.

Despite the progress of the livestock industry in Iran, the production of forage plants commensurate with this development has not been considered. Therefore, this study has been conducted during 2017, with the need to produce feed for livestock and poultry and with the aim of determining the best density and planting date on quantitative and qualitative characteristics of Guar forage. Taking place in the Rasht Rice research Institute-station located in the Village of GilPordehsar in the Sangar city, it evaluates four planting date (May 12, May 26, June 9, and June 23) and three plant densities (200, 400 and 600 plant.ha-1) factorially in a randomized complete block design with three replications. Results show that the planting date of May 26 and the density of 600 plant.ha-1 in terms of fresh forage yield (35.02 t/ha), crude protein (19.02%), dry matter (54.35%), and carbohydrate (26.12%) are superior to other planting dates and plant density under test. The highest yield of dry forage (6.1 t/ha) and neutral detergent fiber (58.23%) has been observed on May 12 and a density of 600 plant.ha-1. The amount of crude fiber has increased with delay in planting, but the percentage of Ash is not affected by different planting dates and densities. According to the results, in order to obtain the highest quantity and quality of forage in Guilan Province, the planting date of May 26 and the density of 600 plant.ha-1 is recommended.

In order to evaluate the effects of planting date and plant density on phenology, seed yield, and essential oil quality of Guar, an experiment has been conducted during 2018-2019 and 2019-2020 growing seasons. It is a split plot layout based on a randomized complete block design with three replications. Different planting dates are considered as the main factor, including September 5th to February 5th as monthly. Three plant densities are considered as sub-factor (20, 40, and 60 plants in a square meter). The required growing degree- day (GDD) from sowing to physiological maturity ranges between 1802.3 and 2347.9 °d on different planting dates. The highest GDD from sowing to physiological maturity is observed on May 5th. The highest seed yield (3780 kg / ha) and galactomannan yield (1050 kg / ha), 100- seed weight (3.5 g), and oil percentage (17.9%) belong to May 5th + 40 plants per m2. by delaying planting time, 100- seed weight causes oil percentage to drop and the seed yield (40%), galactomannan (68%) and the oil percentage (15%) are observed in September.  Therefore, it seems that the best time and density for planting Alyssum is May 5th with a density of 40 plants per m2.

The effects of chemical fertilizer of urea in 5 levels (0, 30, 60, 90 and 150 kg ha-1) and biofertilizers in 4 levels (Nitroxin, Phosphate barvar2, mixed of both and control) on quantity and quality yield of valerian are studied during 2016 to 2020. Measured parameters include valernic acid (percent), valernic acid (g ha-1), root dry weight (kg ha-1), shoot dry weight (kg ha-1), root diameter (mm), root length (cm), leaf width (cm), and leaf length (cm). This study is carried out at the Homad-Absard Agricultural Complex in Damavand city, Tehran Province, Iran, as factorial layout based on a randomized complete blocks design with 3 replications. Results show that the biofertilizers have had a significant effect (p≤0.01) on the all quantity and quality parameters studied. Unlike the urea chemical fertilizer at all levels, which have had a negative effect on the yield and amount of valernic acid (percent), bio-fertilizers has increased it. Maximum and minimum amount of valernic acid (percent) are measured in control and 150 kg ha-1 urea, respectively. The highest root dry weight belongs to interaction of Nitroxin+ Phosphate barvar2+150 kg urea (665 kg ha-1) and control (221 kg ha-1) respectively. Results of this experiment show that biofertilizers with positive effects on the parameters are able to replace the chemical fertilizers.

This study aims at evaluating the yield of cucumber genotypes in two seasons, and identifying cucumber genotypes with high yield and stability. The research has been carried out in the greenhouse of the Department of Horticultural Sciences, University of Guilan, as a factorial experiment in a completely randomized design in autumn and spring seasons with nine cucumber genotypes in three replications. Results show that in terms of number of female flowers in autumn and spring, genotype C1 with an average of 16.7 per plant and genotype C8 with an average of 7.0 per plant have been the best, respectively. Also, in terms of leaf area, C10 genotype with an average of 2866.6 cm2 in autumn and C8 genotype with an average of 9340.990 cm2 in spring outperform the rest and can be used to achieve suitable hybrids for each growing season. Results of principal components analysis and population biplot show that C8 genotype is a suitable genotype for cultivation in two cropping seasons in terms of stability of yield-related traits and vegetative traits. Finally, in terms of number of fruits, genotype C1 with an average of 5.0 per plant in autumn, and C10 genotype with an average of 5.0 per plant in spring have been superior.

This study is conducted to evaluate the concentration of nitrate and heavy metals in soil and edible organs of lettuce in Varamin plain farms for one year during 2017-2018. For this porouse, 30 lettuce fields in Varamin plain are selected and in the lettuce harvest season, soil and crop sampling are performed and the average concentrations of nitrate and heavy metals in the soil and lettuce were compared statistically with the standard limits by one paired t-test. The average concentrations of nitrate, lead and cadmium in lettuce are 2756, 1.46, and 0.11 mg / kg in fresh weight, respectively, which are 1.8, 7.3, and 1.1 times greater than the standard limit, declared by the National Standards Organization of Iran, respectively. The mean concentrations of nitrate and total concentrations of lead, cadmium, and nickel in the soil of lettuce fields are 27, 42, 0.64, and 42.5 mg /kg, respectively, while the concentrations of nitrate and lead have been 35% and 180% higher than the allowable limits, respectively. Risk assessments for heavy metals and non-cancerous diseases are performed, using formulas provided by the US Environmental Protection Agency (USEPA, 2006). In this regard, the risk index for non-cancerous diseases (HQ) is used. The risk factor for non-cancerous diseases for all contaminants is less than one, indicating that there is no risk for consumption. Accordingly, consuming 40 grams of lettuce per day does not pose a problem for an adult consumer. The bioaccumulation index for all the studied metals has been less than one. According to this study, the highest bioaccumulation factor belongs to the heavy metal cadmium, which indicates the tendency of lettuce to absorb and accumulate cadmium compared to other heavy metals. In order to reduce the concentration of nitrate and lead in the soil and prevent contamination of lettuce soils, it is recommended to avoid excessive use of fertilizers containing nitrogen and phosphorus and fertilizer application should be based on soil test. According to the results of this study, there is no serious possibility of exposure to non-cancerous diseases caused by nitrate and heavy metals from eating lettuce, but monitoring the concentration of nitrate and heavy metals in soil and lettuce at different intervals is necessary.

In this study, the tms2 gene, the cause of crown gall, is detected in two Rosa hybrida L. (“Pearl” and “Angelina”) cultivars using electrochemical methods. In this study, stabilization, and hybridization of single-strand DNA (ssDNA) related to tms2 gene, Agrobacterium tumefaciens is investigated by electrochemical impedance spectroscopy. For this purpose, the pencil graphite electrode is modified with nanomaterials including reduced graphene oxide and gold nanoparticles. The biosensor could be used to detect infected samples of “Angelina” and “Pearl” roses. DNA/DNA hybridization is performed by placing an electrode modified with ssDNA at a concentration of two (µmol/L) target DNA. Results show proper stabilization and hybridization of DNA at the electrode surface. The designed biosensor has good selectivity. DNA-based nanosensor has advantages such as low cost, simplicity, and scalability and can be the basis for the development of genomic detection tools.

This research has been done in a completely randomized design and using a factorial experiment with three 3 replications in Date palm and Tropical Fruits Research Center in Ahwaz for two years (2018-2019) to evaluate different methods of date pollen grain maintenance. Factors include type of pollen (fresh pollen, dry pollen, fresh strands, dry strands, and full male spahte cv. Ghannamy, storage environment (room temperature, refrigerator (+4 degrees Celsius) and freezer (-20 degrees Celsius)) and storage time (3, 6, 9 and 12 months). Germination test of pollen grains is performed in liquid culture medium containing 8% sucrose and 50 mg l-1boric acid using a light microscope. Results show that the interaction effects of storage environment× type of pollen and storage environment × storage time on pollen viability have been very significant and dried pollen and dried strand pollen in freezer and refrigerator and during storage period of about one year have given the most appropriate results. Fresh pollen, fresh strand pollen, and whole spahte pollen, due to their high humidity content, lost their viability in a short time. Therefore, in order to preserve the pollen and maintain its vigor and viability in the long run, it is absolutely necessary to dry the strands or pollen in order to reduce their moisture content and increase their shelf life.