مجله دانشگاه علوم پزشکی شهید صدوقی یزد
سال سی و یکم شماره 1 (پیاپی 197، فروردین 1402)

Glucose transporter type 4 (GLUT4) is a membrane glucose transporter that is influenced by insulin signaling and muscle contraction. The aim of the present study was to review the function of GLUT4 in normal conditions and insulin resistance, as well as the role of exercise training on the kinetics of GLUT4 in skeletal muscles. In the present study, by searching for sports keywords; exercise; sports adaptations; glucose transporter; absorption of glucose; glucose transporter type 4; type 2 diabetes; insulin signaling; and insulin resistance was searched from electronic databases of related articles until November 2022 and entered into the study.

Due to the pathological factors of type 2 diabetes, skeletal muscles are disturbed in the delivery, absorption, and metabolism of glucose; pathological disorders of muscle tissue affect insulin signaling and the quantity and function of GLUT4. In the present review, it was found that an exercise training session initiated the GLUT4 gene expression and protein production and its transfer from intracellular stores to sarcolemma; this effect of local exercise was related to muscle contraction. GLUT4 gene and protein expression increased in response to regular exercise and led to an increase in the total level of GLUT4 protein in skeletal muscles; furthermore, regular exercise has improve insulin signaling and, as a result, improved muscle efficiency for blood glucose collection. Considering the role of physical activity on muscle function in blood glucose collection, one of the treatment goals in type 2 diabetes is maintaining muscle mass, increasing muscle mass and also increasing muscle function (muscle quality).

Endometritis is a female reproductive disease characterized by the inflammation of the endometrial glandular and stromal tissues. It is usually occurred due to uterine contamination with infectious causes. Animal models of clinical endometritis are very important to prevent the incidence and treatment of endometritis. Vaginal cytology method is considered as a golden standard for determining the estrous cycle stage in female rats. The aim of this study was to induce endometritis in a rat model and determine the stages of the estrous cycle of this animal using vaginal cytology method in order to successfully induce this condition in this animal model.

Inflammation changes the production of endometrial cytokines, which may result in menstrual disorders and decreased endometrial reception by impaired endometrial tissue dysfunction. Increased expression of specific cytokines genes such as IL-6, IL-8 and TNF-α can be used to detect the severity and degree of endometritis. Uterine infections are known as a result of damage to the defense mechanisms of the uterine tissue, which are caused by physiological changes in the postpartum period. Another change that occurs in the postpartum period is the increase in the production of reactive oxygen species. The imbalance between the production of reactive oxygen species and its clearance leads to the creation of oxidative stress conditions. On the other hand, there is a significant relationship between inflammatory diseases such as endometritis and oxidative stress. On other hand, by accurately determining the estrous cycle of rats by using vaginal cytology, endometritis can be well induced in this species.

Due to the pathophysiology of Sickle cell disease (SCD), several treatment strategies have been reviewed so far. One of the strategies is anti-sickling factors. This study was performed to determine the effect of methanolic extract of Cassia Fistula fruit on sickle cell sickling in vitro.

In this laboratory study, 25 people with sickle cell trait (SCT) ranging in age from 3 to 27 years, and 5 healthy people (as a control) participated. Cassia Fistula was introduced by Dr. Mohammad Taha Jalali to be used in this project. Methanolic extract of Cassia Fistula fruit was obtained by maceration using vacuum distillation (rotary evaporator). Samples with sickle cell trait were examined for sickle cell before and after extract interference, in hypoxic condition. Data analysis was performed by SPSS software version16.

In this study, 64% of the participants were male and 36% were female.  Sickling rates in 1:50, 1: 100 and 1: 200 dilutions were 24%, 37.8% and 46.1%, respectively. According to Wilcoxon Test, the rate of sickling in 1: 2 to 1: 100 dilutions was significantly reduced, relative to pre-interference conditions (P <0.05).

In the present study, it was shown that the methanolic extract of Cassia Fistula plant can prevent erythrocyte sickling in vitro, even with a dilution of 1: 100. According to previous studies, this plant has various therapeutic uses and is non-toxic. Therefore, this extract can be further studied in clinical and in vivo conditions as a useful and cost-effective therapeutic drug.

Human malaria is an infectious-blood disease that is caused by the Plasmodium genus. Anopheles mosquitoes transmit malaria by biting and are well-known as the only biological carriers of this disease. The aim of this research was the identification and introduction of possible inhibitors of Plasmodium falciparum lactate dehydrogenase enzyme using computational techniques of drug design and virtual screening based on macromolecule.

In this analytical-descriptive study, 8733 compounds were initially collected from the PubChem database. In the second step, different filtrations were performed on the library compounds. The selected compounds showed good drug-like properties and pharmacokinetics. Finally, molecular docking simulations were carried out to investigate their binding mode and interactions in the enzyme's active site.

The results of the present study showed that the bonds involved in the binding of the compounds with the enzyme were hydrophobic interactions and hydrogen bonds, and the π-π interaction was in the lower priority. Among the studied compounds, the best docking results were related to the compounds with identification codes CID_23603310, CID_23603337, CID_11912187 and CID_11912184 and free binding energy of -29.10, -9.06, -9.04 and -9.00 kcal/mol, respectively. In general, lipophilic parts and hydrogen bonds increased the affinity and inhibited the enzyme.

Based on the results, all the compounds showed suitable connections in the active site of the enzyme and can be proposed as potential effective inhibitors of Plasmodium falciparum lactate dehydrogenase enzyme.

Nerve inflammation plays an important role in the occurrence and development of diabetic neuropathy pain. The aim of this study was to evaluate the effect of aerobic exercise on anti-inflammatory cytokine IL-35 and behavioral pain responses in a model of diabetic neuropathy rats.

In this experimental study, twenty four 8-week-old male Wistar rats (weight range: 204 ± 11.3 grams) were randomly divided into three groups (n = 8): diabetic neuropathy, diabetic neuropathy exercise and control. After confirmation of diabetic neuropathy pain by hot plate behavioral tests and mechanical allodynia, the aerobic exercise protocol was implemented. IL-35 and NF-κB gene expression in spinal cord tissue was measured by real-time PCR. One-way analysis of variance and Tukey's post hoc test with SPSS version 16 software were used for statistical analysis (P <0.05).

Exercise reduced the response of behavioral tests of diabetic neuropathic pain in the exercise group compared to the diabetic neuropathy group (P <0.05). Induction of diabetes caused a significant decrease in IL-35 gene expression and a significant increase in NF-kB gene (P=0.001) (P=0.021).There was also a significant increase in IL-35 gene expression and a significant decrease in NF-κB gene expression in the exercise group compared to the diabetic neuropathy group (P=0.007) (P=0.034).

It seems that aerobic training has reduced the sensitivity of nociceptors to pain agents by activating the anti-inflammatory cytokine IL-35 in spinal cord microglia. It is recommended to use aerobic exercise to reduce neuropathic pain.

Doxorubicin is a universal drug in treatment of all types of cancers, which is limited in its use due to various side effects in body tissues, so the present study investigated effect of aerobic exercise and use of curcumin nanomicelles on gene expression of the enzymes (GlutathionePeroxidase) GPX, (Catalase) Cat (Superoxidedismutase) SOD and (Malondialdehyde) MDA was investigated in the heart tissue of BalbC female rats with induced breast cancer in the treatment phase of doxorubicin.

This research was practical, it was experimental in terms of control of variables and it was a laboratory in terms of place of implementation. Thirty-six BalbC mice were randomly divided into 6 groups (n=6): healthy, patient, doxorubicin patient, doxorubicin patient and aerobic exercise, doxorubicin patient and nanocurcumin supplement, and doxorubicin patient, aerobic exercise and supplementation. Breast cancer induction was done with MC4-L2 cells. Doxorubicin 2mg/kg was injected subcutaneously 6 times on days 1,7,14,21,28 and 35. Aerobic program consisted of 5 sessions per week for 42 days with an increase in the speed of the treadmill every two weeks, including 14, 16 and 18meters per minute and the use of nanocurcumin at a dose of 100mg/kg.The data collected with SPSS version16 and two-way analysis of variance and for the normality of the frequency distribution with skewness and non-parametric Kolmogorov-Smirnov test were performed with a significance level of P ≤ 0.05.

Six weeks of aerobic training had no effect on the expression of GPX, MDA and SOD genes in the target groups, but it significantly increased the expression of Catgene (P ≤ 0.05). The use of nanocurcurmin supplement for 6 weeks decreased the expression of MDA gene and significantly increased the expression of GPX, SOD and Catgenes. Six weeks of aerobic training along with nanocorcurmin supplementation significantly increased Cat gene expression (P≤0.05), but had no effect on GPX, MDA and SOD gene expression.

Aerobic exercises and the use of nanocurcurmin supplement separately or at the same time can be effective in reducing the cardiac toxicity of doxorubicin in the patients with breast cancer.

In the recent years, transcranial direct current stimulation (tDCS) has been considered by researchers as one of the possible effective approach in the treatment of major depressive disorder. However, the results of these investigations are often contradictory. According to this, the purpose of this study was to determine the effectiveness of tDCS in reducing symptoms of people with major depressive disorder.

In the sham-controlled randomized trial, twenty people with major depressive disorder referred to a private psychiatric clinic in Tehran City, Iran. They were selected by convenience sampling and divided into the intervention and control groups by randomized block design. tDCS was administered 2 mA, 20 minutes per session, for 10 sessions, 3 days per week. Immediately after, and one month after the intervention, the scores of the two groups were assessed in the Hamilton Depression Rating Scale and Beck Depression Inventory. The data were analyzed using SPSS version 16 software and using statistical methods of repeated analysis of variance and independent and dependent t-test.

The results of repeated analysis of variance for Hamilton (F = 43.5 and P = 0.017) and Beck (F = 125.12 and P = 0.022) depression scores showed the significance of the interaction effect of time (four stages of measurement) and group membership (experiment and control) (P < 0.05). In depression scores in both Hamilton and Beck scales in the experimental group in the intervention stages (Hamilton scale, P = 0.019, Beck scale = P = 0.017) and follow-up (Hamilton scale, P = 0.01, Beck scale = P = 0.03) was significantly reduced compared to the control group.

The results of this study confirmed the effectiveness of tDCS in reducing symptoms of major depressive disorder. More clinical trials are needed to prove the effectiveness of tDCS compared to a variety of treatments

This prospective analytical study was conducted with the aim of determining the prevalence, severity and factors affecting the short-term complications of implant surgery.

In a descriptive cross sectional study, patients referring to the implant department of Tehran University of Medical Science from 2020 to 2021 were included (292 implants in 152 patients). Patients underwent Simple implant placement without hard and/or soft tissue grafts were included. After 7 and 14 days, ecchymosis, bleeding, swelling, delayed wound healing, infection, and trismus were recorded. Pain, the most common outcome after surgeries was assessed based on the number of days the patient had pain, the number of analgesics used and the severity of pain (Numeric pain intensity scale) using SPSS software version 16. The relationship between complications and measured variables was examined by logestic regression analyses.

The most common complications on the 7th day follow up were ecchymosis (3.9%) and delayed wound healing (3.9%). The mean pain score was 1.61 ± 1.32. No patient had pain more than 3 days after surgery. The mean number of analgesic used was 2.23 ± 1.82. Based on logistic regression analyses, only patient age and the number of days of analgesics use showed a significant relationship with the number of analgesics used (P˂0.001). Neither the level of expertise of the surgeon nor the patients' gender had nosignificant effect on the degree of complications pain and amount of patient pain and the consumption of analgesics (P˃0.05). Patients with poor oral hygiene used more analgesics in comparison to patients with favorable oral hygiene.

The overall prevalence of post-operative complications after simple implant surgeries was less than 4%. The older the patient, the higher the consumption of analgesics.