فهرست مطالب
Govaresh
Volume:28 Issue: 1, Spring 2023
- تاریخ انتشار: 1402/03/30
- تعداد عناوین: 9
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Pages 6-12Background
The aim of this study was to investigate the response rate to the hepatitis B vaccine and the factors affecting it in people with advanced kidney failure undergoing hemodialysis.
Materials and MethodsThis cross-sectional study was conducted from 2019 to 2021 at Ziyaian Hospital in Tehran. For patients undergoing hemodialysis, serum levels of the nutritional, inflammatory, and bone and mineral markers were measured before hepatitis B vaccine injection. They then received hepatitis B vaccine, and 6 months later the serum level of hepatitis B antibody was measured. In case of no immune response to the vaccine, a second injection was given. Then the rate of lack of immune response to the vaccine in the subjects under study was determined and the levels of nutritional, inflammatory, and bone factors were compared between those who responded to the vaccine and those without an immune response.
Results54 people were included in the study. In the first round of vaccination, 45 people participated, of whom 62.2% responded to the vaccine and 37.8% did not respond. 26 people participated in the second round of vaccination, in which 69.2% (18 people) responded to the vaccine and 30.8% (8 people) did not respond to the second round of the vaccine. In the non-response group to the vaccine, the serum levels of albumin, and cholesterol were significantly lower and the serum level of neutrophils and the ratio of neutrophils to lymphocytes was significantly higher than in the patients with response to the vaccine.
ConclusionInflammatory and nutritional factors can affect the immune response to hepatitis B vaccine. Therefore, it is recommended to check the nutritional status of these patients and encourage them to follow up on the results of the vaccine.
Keywords: Hepatitis B vaccine, Vaccine immunogenicity, Advanced kidney failure -
Pages 13-18Background
Considering that no study on the efficacy of dexmedetomidine during endoscopic retrograde cholangiopancreatography (ERCP) has been performed in Iran so far, we decided to evaluate the efficacy and effectiveness of dexmedetomidine and patient satisfaction in patients undergoing ERCP.
Materials and MethodsA double-blind clinical trial was performed (2020) among 80 patients undergoing ERCP grade ASA I, and II who had no asthma, cardiovascular diseases, and no history of allergies to propofol and dexmedetomidine. The patients were randomly divided into two groups (fentanyl and propofol) and (fentanyl and dexmedetomidine). Their heart rate per minute, mean arterial pressure, respiration rate per minute, and blood oxygen saturation at intervals of 5 and 15 minutes after the start and end of the procedure, the level of patient cooperation, and endoscopies satisfaction were compared.
ResultsMean age of the propofol group was 63/13 ± 17/88 and the dexmedetomidine group was 16/99 ± 58/55 (P = 0.191). Mean heart rate and respiration rate per minute were not different significantly in any of the time intervals. Mean arterial pressure in the propofol group before, 5, and 15 minutes after the start and end of ERCP were higher than dexmedetomidine group (105/95 ± 15/91, 123/75 ± 25/54, 118/10 ± 13/06 and 14/61 ± 117/50, respectively). The percentage of oxygen saturation at 5 minutes and at the end of the procedure was higher in the propofol group (P = 0.01 and P = 0.03, respectively)
ConclusionDue to fewer side effects and more satisfaction of patients receiving propofol; this drug is preferred for these patients.
Keywords: ERCP, Propofol, Dexmedetomidine -
Pages 19-29Background
In this study, we investigated the expression profile of this disease to identify new hub genes to help diagnose hepatocellular carcinoma (HCC).
Materials and MethodsWeighted gene co-expression network (WGCNA) analysis was used in this study to identify key modules and hub genes associated with HCC in the GSE176271 dataset. We also looked at the clinical significance of key genes and the biological pathways linked to them in external databases. We validated the identified hub genes using data from the GEPIA and XenaBrowser databases.
ResultsThe Midnight blue module was found to be significantly related to the pathological stage (r = 0.94, P = 1e-11). Five hub genes (CLEC4M, CLEC4G, FCN2, OIT3, and ASPG) were associated with prognosis using DEG identification and WGCNA analysis. The three biological pathways associated with the Midnight blue module were copper ion detoxification, cell ion homeostasis, and complement activation, as well as the lectin pathway.
ConclusionThe current study's findings provide new and effective molecular targets for the detection of HCC, which can improve patients’ prognosis.
Keywords: Carcinoma, Hepatocellular, Biomarker, Diagnosis, Weighted gene co-expression network analysis (WGCNA) -
Pages 30-35Background
The current study aimed to investigate the prevalence, risk factors, endoscopic findings, and outcome of gastrointestinal bleeding in COVID-19 hospitalized patients.
Materials and MethodsThis study is a retrospective review of COVID-19 hospitalized patients with gastrointestinal bleeding from a large university hospital in southeast Iran. This study was conducted over one year from April 2020 to March 2021.
ResultsOut of 3563 COVID-19 inpatients with approximately equal sex distribution (52.5% of men and 47.5% of women), 80 (2.24%) patients with a mean age of 58.01 ± 20.71 were identified with signs of gastrointestinal bleeding, including; melena (48.8%), hemoglobin drop (42.5%), fresh blood hematemesis (31.3%), rectorrhagia (20%) and coffee ground emesis (10%). 52 patients (65%) had signs of gastrointestinal bleeding on admission, and 28 patients (35%) developed gastrointestinal bleeding during their hospital admissions, most of whom were men (63.8%). Endoscopic characteristics were; gastric erosion (27.7%), gastric ulcer (23%), duodenal ulcer (21.5%), esophageal ulcer (12.3%), and esophageal erosion (6.1%) in upper gastrointestinal endoscopy. On colonoscopy, hemorrhoids (25%), mass lesions (16%), fissures (8.3%), diverticular lesions (8.3%), and polyps (8.3%) were the most common findings. The overall mortality of the patients in the present study was 36.2%. However, gastrointestinal bleedingrelated deaths were 7.5%.
ConclusionGastrointestinal bleeding was identified in 2.24% of hospitalized COVID-19 patients, with gastroduodenal ulcers and erosions as the most common symptoms.
Keywords: COVID-19, Gastrointestinal Tract, Hemorrhage, Endoscopy, Colonoscopy, Iran -
Pages 36-41Background
Celiac disease (CD), or as it is called gluten enteropathy, could result in various complications such as liver abnormalities. Nonalcoholic fatty liver disease (NAFLD) affects 25.2% of individuals worldwide and has become one of the most common causes of cirrhosis. This study aimed to report the frequency of fatty liver in ultrasonography of patients with CD in Golestan province, Northeast Iran.
Materials and MethodsIn this cross-sectional study, patients diagnosed with CD based on a positive anti-tTG Ab and proved by small bowel biopsy were recruited between March 2018 and March 2020 (N = 80), through the registry system located in Golestan Research Center of Gastroenterology and Hepatology, Northeast Iran. Ultrasonography was performed and the fatty liver diagnosis was done based on attenuation of the liver itself (less than 40 Hounsfield Unit (HU)) or in comparison with the spleen (liver attenuation ≤ 10, than spleen).
ResultsAmong 80 patients with CD (46 women, mean age 39.13 ± 12.5 years), fatty liver was reported in 34 (43.5%), which was mild in 31 (39%), moderate in 2 (2.5%), and severe in 1 (1.3%) patients. One cirrhosis and no hepatomegaly were found.
ConclusionIn the present study, fatty liver was seen in 43.5% of our patients, which is a large amount. It may be suggested to do a closer follow-up of the complications of CD.
Keywords: Celiac disease, Fatty liver disease, Gastroenterology -
Pages 42-49Background
Celiac disease (CD) is one of the most common and chronic immune-mediated disorders of the gastrointestinal tract that affects the small intestine. Genetic factors, including human leukocyte antigen (HLA), and non-HLA genes are major risk factors in CD pathology. Moreover, environmental factors such as infections may affect CD incidence.
Materials and MethodsThe goal of the present study is to investigate the association between CD and viral, bacterial, fungal, and parasitic infections. Databases including Scopus, PubMed, Google Scholar, and Web of Science were searched for relevant literature until 2022.
ResultsSeveral infections have been reported to be associated with CD including reovirus, rotavirus, hepatitis B virus, influenza virus, helicobacter pylori, campylobacter jejuni, giardia lamblia, toxoplasma gondii, candida albicans, etc.
ConclusionMoreover, antibiotic administration might be a risk factor for further CD development. On the other hand, there are reports regarding the susceptibility of patients with CD to some infections, as well as protective infections against CD. Consequently, more studies are required to explain the two-sided relations between CD and infectious diseases.
Keywords: Celiac, Infection, Risk factor, Immune-mediated disease, Antibiotics -
Pages 50-57Background
There is increasing evidence that surgical approaches to weight loss (bariatric surgery) are the most effective and steady treatment for morbidly obese patients. This study assessed the nutritional status of obese participants candidates for laparoscopic gastric plication (LGP) before and after the surgery.
Materials and MethodsOur study was conducted on 38 participants aged 18 to 65, with a mean body mass index (BMI) of 41.45 ± 5.68 kg/m2, scheduled for LGP. BMI and Dietary intakes were assessed at baseline and 6 months after surgery. The mean daily energy intake and microand macronutrients were calculated and compared with the dietary reference intake each time.
ResultsThe mean excess weight loss was 44.45 ± 9.78%. The mean daily energy intake decreased to one-third of the baseline 6 months after surgery. All patients had a low intake of protein, vitamins A, D, and folic acid before and after LGP. After surgery, over 60% of patients had inadequate vitamin B1, B2, B3, B6, calcium, and iron levels.
Conclusion6 months after LGP, our results demonstrated dietary deficiencies, including inadequate protein and certain micronutrients. Some of these nutritional deficiencies existed before surgery and persisted or worsened after surgery. In order to ensure optimal health before and after surgery, patient care should place a strong emphasis on nutritional counseling and supplementation.
Keywords: Micronutrients, Bariatric surgery, Dietary intake, Laparoscopic gastric plication -
Pages 58-65Background
Hepatocellular carcinoma (HCC) ranks as the first and the second malignancy in men and women in Egypt. Many inflammatory markers are included in HCC pathogenesis. Matrix metalloproteinase-14 (MMP-14) is one of them and it is implicated in regulating HCC tumor progression and prognosis. This study was done basically to assess the frequency of alleles of rs2236307 single nucleotide polymorphism (SNPs) of the MMP-14 ( + 7096) gene in patients with HCC and to determine if there is an association between its alleles polymorphism and the occurrence of HCC in Egyptian patients.
Materials and MethodsA case-control study was done in the Tropical Medicine Department at Zagazig University Hospitals and the Medical Biochemistry Department, Faculty of Medicine, Zagazig University. The study included 540 subjects, classified into three groups HCC, cirrhotic, and controls (180 in each group). MMP-14 gene polymorphism analysis in the promoter of the MMP-14 gene rs2236307 was detected by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP).
ResultsThe TC genotype was significantly higher among patients with HCC when compared with the control group (77.8% versus 16.7%, respectively). The TC genotype was higher among patients with HCC compared with the cirrhotic group (77.8% versus 38.9%, respectively). Metastatic lesions and portal vein thrombosis were significantly higher among the TC group compared with the CC and the TT groups in the HCC group.
ConclusionThe polymorphisms of MMP-14 rs2236307 increase the risk of HCC. Both the TC and the CC genotypes showed HCC risk association but the the CC genotype appeared with lower potential.
Keywords: Hepatocellular carcinoma, MMP-14 ( + 7096) rs2236307, TC genotype, CC genotype -
Pages 66-77Background
Hepatitis C virus (HCV) infection affects around 170 million individuals worldwide, with an estimated 3% of the world’s population presently afflicted. More than 350,000 people are killed each year by HCV throughout Asia and the rest of the globe due to liver disorders such as cirrhosis, chronic hepatitis, and hepatocellular carcinoma (HCC). Understanding viral-host protein interactions are essential for understanding viral infection, disease etiology, and the development of innovative therapeutics. This is due to the inherent limits of laboratory techniques for finding host-virus protein-protein interactions (PPIs). There seems to be a strong computational effect on the research of cellular infection.
Materials and MethodsIn this study, we predicted the interaction between human and HCV proteins using an ensemble learning technique.Support vector machines (SVMs) nuclear liner and radial are the cornerstones of our model, as are K-Nearest Neighbors (KNN) and Random Forest (RF). Four different feature vectors were used to encode human and HCV proteins: the tripeptide composition (TPC), The composition of k-spaced acid pairs (CKSAAP), the amino acid autocorrelation-autocovariance (AAutoCor), and the conjoint triad (CT).
ResultsThe predictive power of the suggested technique is evaluated using a benchmark dataset that contains both consistently positive and negative PPIs. A support vector machine (Radial-SVM) model was used to predict which human proteins interact with HCV. To achieve accuracy and specificity of 84.9 and 88.3 percent, we employed tenfold cross-validation and principal component analysis (PCA).
ConclusionOur technique correctly predicts PPIs based on human and HCV proteins. The discovery of HCV-human protein interaction networks, enriched pathways, gene ontology, and functional categories has improved our knowledge of HCV infection.
Keywords: HCV, PPIs, Computational, SVM, Prediction