فهرست مطالب

Iranian Biomedical Journal - Volume:28 Issue: 2, May 2024

Iranian Biomedical Journal
Volume:28 Issue: 2, May 2024

  • تاریخ انتشار: 1402/12/11
  • تعداد عناوین: 8
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  • Nikita S. Voronkov, Sergey V. Popov, Natalia V. Naryzhnaya, N. Rajendra Prasad, Ivan M. Petrov, Viktor V. Kolpakov, Evgenia A. Tomilova, Ekaterina V. Sapozhenkova, Leonid N. Maslov* Pages 59-70

    Despite the unconditional success achieved in the treatment and prevention of AMI over the past 40 years, mortality in this disease remains high. Hence, it is necessary to develop novel drugs with mechanism of action different from those currently used in clinical practices. Studying the molecular mechanisms involved in the cardioprotective effect of adapting to cold could contribute to the development of drugs that increase cardiac tolerance to the impact of ischemia/reperfusion. An analysis of the published data shows that the long-term human stay in the Far North contributes to the occurrence of cardiovascular diseases. At the same time, chronic and continuous exposure to cold increases tolerance of the rat heart to ischemia/ reperfusion. It has been demonstrated that the cardioprotective effect of cold adaptation depends on the activation of ROS production, stimulation of the β2-adrenergic receptor and protein kinase C, MPT pore closing, and KATP channel.

    Keywords: Acclimatization, Cold temperature, Heart, Ischemia, Reperfusion
  • Sejal S. Shah, Bhavika P. Turakhia, Nihar Purohit, Khushal M. Kapadiya, Chita R. Sahoo, Akhilesh Prajapati* Pages 71-81
    Background

    Bioreductive processes are quite potent, effective and affordable for the synthesis of green NPs, as compared to the physical and chemical methods. The present study aimed to evaluate the bactericidal, antioxidative and anticancer activity of FeONPs derived from the turmeric rhizome (Curcuma amada) using ferric chloride as a precursor.

    Methods

    With focusing on the manufacture of FeONPs via  green approach, we characterized the NPs using FTIR, FT-Vis, DLS, and UV-Vis spectroscopy. The produced particles were tested for antibacterial, antioxidant, and anticancer properties. The synthesized NPs were also examined using the MDA-MB-231 human epithelial breast cancer cell line and NCI-60 cancer cell lines.

    Results

    The antioxidant activity of TR-FeONPs was concentration-dependent. The scavenging activity of TR-FeONPs was 76.09% at a concentration of 140 µg/ml. Using different concentrations of TR-FeONPs in the MTT assay against the MDA-MB-231 cell line indicated a reduction of less than 50% in cell viability at 125 µg/ml. Moreover, TR-FeONPs exhibited an effective bactericidal property. The gTR-FeONPs synthesized bioreductively were found to be effective in renal cancer, UO-31 cell line, with GI50 value of 66.64%.  

    Conclusion

    Our study showcases a sustainable method based on green chemistry principles to produce FeONPs utilizing turmeric rhizome. We anticipate that the FeONPs produced through this biosynthesis process could serve as a promising drug delivery system in cancer treatment
    and as an effective antimicrobial agent against various diseases.

    Keywords: Antioxidants, Bactericidale activity, Green Synthesis, MDA-MB-231cells, Molecular docking
  • Nawar Bahjet Kamil*, Nada M.H. AL-Ghaban Pages 82-89
    Background

    Osteogenic, antioxidant and anti-inflammatory effects of Whey protein and M. oleifera gel prompted us to evaluate their role alone or in combination on osseointegration in rabbits.

    Methods

    In this study, 24 titanium implants were inserted in the femurs of six rabbits. One implant was placed without treatment, and another one was coated with a mixture of whey protein and M. oleifera gel for each side.  The animals were divided into two groups of 2- and 6-week intervals and evaluated using histopathological and immunohistochemical techniques.

    Results

    Histological evaluation revealed a significant difference between the experimental and the control groups after two weeks in osteoblast and osteocyte counts. The experimental group had mature bone development after six weeks of implantation, while the control group had a woven bone. Immunohistochemical results showed that the experimental group, compared to the control group, exhibited early positive expression of osteoblast cells at two weeks after the experiment. Based on histopathological observations, the experimental group showed a tiny area of collagenous fiber in 6th week after the implantation.

    Conclusion

    A mixture of whey protein and M. oleifera could accelerate osseointegration and healing processes.

    Keywords: Dental implants, Insulin-like growth factor I, Moringa oleifera, Osseointegration, Whey proteins
  • Armaghan Gheytasvand, Hamed Bagheri, Shahram Pourbeyranvand, Mojdeh Salehnia* Pages 90-101
    Background

    Synthetic and natural polymer scaffolds can be used to design wound dressing for repairing the damaged skin tissue. This study investigated acute wound healing process using a decellularized skin scaffold and MEF.

    Methods

    Mouse skin fragments were decellularized and evaluated by DNA content, toxicity, H&E staining, Raman confocal microscopy, Masson’s trichrome staining, SEM, and biodegradation assays. The fragments were recellularized by the MEFs, and cell attachment and penetration were studied. De- and decellularized scaffolds were used wound dressings in mouse acute wound models as two experimental groups. Using morphological and immunohistochemical assessments, wound healing was evaluated and compared among the experimental and control groups.

    Results

    DNA content of the decellularized tissue significantly reduced compared to the native control group (7% vs. 100%; p < 0.05). ECM components, e.g. collagen types I, III, and IV, elastin, and glycosaminoglycan, were well preserved in the decellularized group. The porosity and fiber arrangement in the stroma had a structure similar to normal skin tissue. A significant reduction in healing time was observed in the group treated with a decellularized scaffold. A thicker epidermis layer was observed in the recovered tissue in both experimental groups compared to the control group. Immunostaining showed a positive reaction for CD31 as an endothelial marker in both experimental groups, confirming new vascularization in these groups.

    Conclusion

    Using MEFs with decellularized skin as a wound dressing increases the rate of wound healing and also the formation of new capillaries. This system could be beneficial for clinical applications in the field of tissue engineering.

    Keywords: Decellularized extracellular matrix, Neovascularization, Skin, Wound healing
  • Elmira Karimzadeh Barenji, Shokufeh Beglari, Azar Tahghighi, Parisa Azerang*, Mahdi Rohani* Pages 102-112
    Background

    Lactic acid bacteria produce various beneficial metabolites, including antimicrobial agents. Owing to the fast-rising antibiotic resistance among pathogenic microbes, scientists are exploring antimicrobials beyond antibiotics. In this study, we examined four Lactobacillus strains, namely L. plantarum 42, L. brevis 205, L. rhamnosus 239, and L. delbrueckii 263, isolated from healthy human microbiota, to evaluate their antibacterial and antifungal activity.

    Methods

    Lactobacillus strains were cultivated, and the conditioned media were obtained. The supernatant was then used to treat pathogenic bacteria and applied to the growth media containing fungal and bacterial strains. Additionally, the supernatant was separated to achieve the organic and aqueous phases. The two phases were then examined in terms of bacterial and fungal growth rates. Disk diffusion and MIC tests were conducted to determine strains with the most growth inhibition potential. Finally, the potent strains identified through the MIC test, were tested on the pathogenic microorganisms to assess their effects on the formation of pathogenic biofilms.

    Results

    The organic phase of L. rhamnosus 239 extracts exhibited the highest antibacterial and antibiofilm effects, while that of L. brevis 205 demonstrated the most effective antifungal impact, with a MIC of 125 µg/mL against Saccharomyces cerevisiae.

    Conclusion

    This study confirms the significant antimicrobial impacts of the LAB strains on pathogenic bacteria and fungi; hence, they could serve as a reliable alternative to antibiotics for a safe and natural protection against pathogenic microorganisms.

    Keywords: Biofilms, Lactobacillus, Drug resistance
  • Hanieh Jahi, Mansoureh Eslami*, Mohammad Sayyah*, Fariba Karimzadeh, Melika Alesheikh Pages 113-119
    Background

    Traumatic brain injury or TBI can underlie epilepsy. Prevention of PTE has been of great interest to scientists. Given the antiepileptic, antioxidant and anti-inflammatory activities of curcumin, we examined whether this compound can affect epileptogenesis in rats after TBI.

    Methods

    Curcumin was injected once a day for two weeks. TBI was induced in the temporal cortex of anesthetized rats using a CCI device. One day after TBI, PTZ, 35 mg/kg, was injected i.p. every other day until manifestation of generalized seizures. The number of PTZ injections was then recorded. Moreover, the extent of cortical and hippocampal IL-1β and GFAP expression in the epileptic rats were measured by Western blot analysis.

    Results

    Curcumin 50 and 150 mg/kg prevented the development of kindling, wherase TBI accelerated the rate of kindling. Curcumin 20 mg/kg prohibited kindling facilitation by TBI, and reduced the expression of IL-1β and GFAP induced by TBI.

    Conclusion

    Curcumin can stop the acceleration of epileptogenesis after TBI in rats. Inhibiting hippocampal and cortical overexpression of IL-1β and GFAP seems to be involved in this activity.

    Keywords: Glial fibrillary acidic protein, IL-1β, Post-traumatic epilepsy, Traumatic brain injury
  • Aida Albadawy, Mohammed Alqudaimi, Hanyue Cui, Yan Xianghui, Jing Sun, Ping Shi* Pages 120-130
  • Abdulwahab Teflischi Gharavi, Azadeh Niknejad, Saeed Irian, Amirabbas Rahimi, Mona Salimi* Pages 132-139
    Background

    ExoRNAs offer valuable insights into their cellular origin. ExoRNA studies were faced with challenges in obtaining sufficient amounts of high-quality RNA. Herein, we aimed to compare three traditional exosome isolation methods to introduce an appropriate strategy to extract RNA from cancer-derived exosomes for further RNA analysis.

    Methods

    Exosomes were isolated through ultracentrifugation, precipitation kit, and size exclusion column chromatography, and then characterized by DLS and TEM, followed by extracting total RNA. The quality and quantity of the extracted RNAs were assessed by a NanoDrop and 2.5% agarose gel electrophoresis.

    Results

    Extracted exosomes displayed a similar range of size and morphology. We found that PEG-precipitation method resulted in a higher RNA yield with a 260/280 ratio of 1.9. The obtained exoRNA appeared as a smear in the agarose gel, indicative of small exoRNAs.

    Conclusion

    We provide researchers a suitable approach to isolate exosomes based on yield and purity of exoRNA.

    Keywords: Exosomes, Extracellular vesicles, MicroRNA