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Chemical Methodologies - Volume:8 Issue: 5, May 2024

Chemical Methodologies
Volume:8 Issue: 5, May 2024

  • تاریخ انتشار: 1403/02/12
  • تعداد عناوین: 5
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  • Somayeh Tajik *, Fariba Garkani Nejad, Reza Zaimbashi, Hadi Beitollai Pages 316-328
    In this study, MoSe2/rGO nanocomposite modified screen printed graphite electrode (SPGE) was designed for acyclovir (ACV) determination. The electrochemical investigation and measurement of ACV were performed by applying some voltammetric techniques and chronoamperometry. After modification of SPGE, the enhancement of the voltammetric response and the reduction of overpotential of ACV confirmed the good electrocatalytic ability of MoSe2/rGO/SPGE sensor towards the ACV oxidation. The voltammetric method (differential pulse voltammetry (DPV)) was used to investigate the determination ability of MoSe2/rGO nanocomposite/SPGE towards ACV determination under the optimum parameters and conditions. The MoSe2/rGO/SPGE sensor indicated appreciable sensing ability towards ACV, with an optimal linear response from 0.03-190.0 µM and a limit of detection (LOD) of 0.01 µM. More importantly, the practical applicability of the designed sensor was confirmed in the ACV quantification in ACV tablet and urine samples, showing its potential application for real sample analysis.
    Keywords: Screen Printed Graphite Electrode, Electrochemical Sensor, Mose2, Rgo Nanocomposite, Acyclovir
  • Olia Rezaeianzadeh, Sakineh Asghari *, Mahmood Tajbakhsh, Mojtaba Mohseni, Asieh Khalilpour Pages 329-350
    We synthesized a series of new azo-based sulfonamides 8a-l via multistep chemical processes including chlorosulfonation, nucleophilic substitution, diazotization, and coupling reactions. The synthesized compounds were characterized using various physical and spectral techniques such as melting point, IR, 1H- and 13C-NMR, Mass, and elemental analysis. We evaluated the antibacterial and anticancer activities of compounds 8a-l. The cytotoxicity of these compounds was assessed on the MCF-7 breast cancer cell line and the MCF-10 human normal cell line after 48 h exposure. Notably, compound 8h demonstrated significantly higher cytotoxicity against MCF-7 (IC50 = 0.21 µM) while showing minimal toxicity towards the MCF-10 human normal cell line. To gain insights into the molecular interactions, we utilized molecular docking to predict the binding affinity of these compounds to the FGFR2 kinase receptor structure (PDB ID: 4J98). Compound 8h exhibited the highest docking score, consistent with our experimental results and demonstrating favorable protein-substrate interactions. In addition, we performed ADME prediction of the compounds, indicating their potential as lead drug candidates. Furthermore, we evaluated the antibacterial activity of compounds 8a-l against Gram-positive and Gram-negative bacteria. Compound 8i showed the strongest antibacterial activity against Staphylococcus aureus, a Gram-positive pathogen. This study provides valuable insights into the biological activities of azo-based sulfonamide derivatives, establishing their potential as both anticancer agents and antibacterial compounds.
    Keywords: Azo-Based Sulfonamide, Sulfonamide, Breast Cancer, Molecular Docking, Cytotoxicity, Antibacterial
  • Mohsen Samimi *, Khadijeh Amiri Pages 351-363
    Spherical alginate-based hydrogels have been applied for environmental pollutants removal from aqueous solutions. In this study, zinc alginate beads were prepared using an ionotropic gelation process. The wet hydrogels prepared in various operational conditions were studied to remove eosin-B dye. The effect of operational variables such as pH, initial concentration of eosin-B, contact time, dose of synthesized hydrogel beads, and environmental temperature on the eosin-B removal process was evaluated. The maximum dye removal efficiency was obtained by zinc alginate biosorbent at pH = 3, contact time of 40 min, and 35 mg of biosorbent dose. The maximum adsorption capacity of zinc alginate beads for eosin-B uptake was 53.48 mg/g of biosorbent. The mechanism, kinetics, isotherms, and thermodynamic studies of eosin-B sorption by zinc alginate hydrogels were investigated. Based on the results, the biosorption process follows pseudo-second-order kinetics. The experimental data of the biosorption process are matched with the Langmuir isotherm model, suggesting that monolayer adsorption plays a vital role in the analyte biosorption. According to the thermodynamic studies, the parameters ∆H° (+4679.12 J.mol-1), ∆G° (from -173.45 to -499.13 J.mol-1), and ΔS° (+16.284 J.mol-1.K-1) confirmed the endothermic, spontaneity and randomness of the biosorption process, respectively. This study shows that zinc alginate can be a suitable biosorbent for removing eosin-B from aqueous solutions.
    Keywords: Biosorbent, Eosin-B, Isotherms, Kinetics, Thermodynamic Parameters, Zinc Alginate
  • Tahereh Sangchooli, Sobhan Aboulhassanzadeh, Hamed Aghazadeh, Marzieh Paeizi *, Dariush Shokri, Maryam Malekzadeh Pages 364-385
    Ferula assa-foetida L. (Asafoetida) a medicinal plant with antimicrobial and antioxidant properties, is crucial in the food and pharmaceutical industries. Ferula essential oils (EOs), rich in ferulic acid, flavonoids, alkaloids, and glycosides compositions, may offer a potential solution to antibiotic resistance. The aim of this work is to investigate the components of Ferula chemical essential oil (EO) and its antioxidant and antimicrobial properties in different habitats of Iran (Tabas, Yazd, Neishabur, and Kerman). Gas Chromatography-Mass Spectrometry (GC-MS) analysis was performed to determine the composition of the essential oils from the four different sources. The antimicrobial activity, the minimum inhibitory concentration (MIC), and the minimum bactericidal concentration (MBC) of the essential oils against both Gram-positive and Gram-negative pathogenic bacteria were evaluated using the broth microdilution method and the well diffusion test. Likewise, the antioxidant properties of the essential oils were examined using the DPPH radical scavenging method. GC-MS analysis identified the major components of the essential oils, with the Nishabur EO having the highest percentage of components. The Kerman EO had the lowest inhibitory concentration against Escherichia coli, at 6.25 mg/ml, while the Yazd EO demonstrated the highest inhibitory concentration against Staphylococcus aureus, at a minimum inhibitory concentration of 50 mg/ml. Yazd EO showed the strongest antimicrobial activity, particularly against Gram-positive bacteria, with a halo diameter of 22.5 mm. In contrast, the Kerman EO showed the least amount of antimicrobial activity against Escherichia coli, with a halo diameter of 11.5 mm. Kerman EO had the highest antioxidant activity, with 93.9393%. The Ferula EO has the ability to inhibit DPPH radicals and demonstrated activity against both Gram-positive and Gram-negative bacteria, making it a strong antioxidant and natural preservative in the pharmaceutical industry.
    Keywords: Ferula Assa-Foetida, GC-MS, MIC, MBC, Antimicrobial Test, Antioxidant
  • Yalda Jahanbani, Soodabeh Davaran *, Mehdi Yousefi, Leila Roshangar, Parvin Bastani, Jamileh Kadkhoda Pages 386-400
    Development of new biomaterial-based approaches for regeneration of soft tissues and organs such as heart, brain, uterine, ovarian, and others has received much attention in recent years. Here, we explain the stages of design and development of the biocompatible Collagen/Polyvinyl alcohol (COL/PVA) nanofiber scaffolds to transfer human umbilical cord mesenchymal stem cells (HUC-MSCs) to damaged soft tissue. In this study, by optimizing the percentage ratio of COL to PVA, the need for a cross-linking process to maintain the nanofibers' stability in aqueous environments was eliminated and this strategy significantly increased the biocompatibility of the synthesized nanofibers. The chemical structure of synthesized scaffolds was evaluated by Fourier-transform infrared spectroscopy (FT-IR). In addition, other physicochemical and biological aspects of the fabricated scaffolds, including nanofiber diameter, in vitro degradation, swelling behavior, mechanical properties, morphologies, and biocompatibility were surveyed. Physiochemical assessments showed that un cross linked 60/40 COL/PVA nanofiber scaffolds had a successful performance in terms of morphology and stability. Furthermore, these scaffolds had no toxicity on HUC-MSCs. Therefore, study was continued with the most ideal percentage composition of the prepared nanofiber scaffolds. Scanning electron microscope (SEM) images showed proper cell adhesion and distribution of HUC-MSCs throughout the nanofiber scaffolds.
    Keywords: Soft Tissue Engineering, Electrospinning, Nanofiber Scaffolds