مجله پژوهش های سلولی مولکولی (زیست شناسی ایران)
سال سی و هفتم شماره 2 (تابستان 1403)

Heat shock proteins (HSPs) play an important role in responding to various environmental stresses. In this study, genomic and proteomic characteristics of HSP90-a and HSP90-b in Tali goats in addition to 7 species of domestic animals including sheep (O. aries), goat (C. hircus), water buffalo (B. bubalis), cattle (B. taurus), Zebo cattle (B. indicus), one-humped camel (C. dromedaries) and two-humped camel (C. bactrianus) were analyzed using Multiple sequence alignment, SWISS modeling and phylogenetics analysis tools. The location of HSP90-a and HSP90-b gene is determined in all of studied species but its position has not been determined in the two humped camels. The number of exons for HSP90-a and HSP90-b in all studied species except two camel species were similar. Comparison of mutations in the Tali breed with the reference goat showed that the mutations of the exon regions have not caused any change in amino acids. Analysis of the Expasy translation tool also showed that HSP90-a and HSP90-b encode 733 and 724 amino acids for all species, respectively, except for the two-humped camel, which has 649 amino acids for the HSP90-a protein. The results showed that HSP90-a protein had one variable amino acid in water buffalo and six variable amino acids in two-humped camel, while only one-humped camel and two-humped camel had two variable amino acids in HSP90-b protein. This study will be supportive in selection of target genes for molecular adaptation of livestock.

Tamarix is belonging to the Tamaricaceae family with 13 indigenous species of Iran and is one of the important ornamental plants in the landscape. This plant has been considered due to its resistance to the different weather and compatibility with salinity condition. In this study was conducted to investigate genetic diversity of different tamarix genotype endemic in the Ardebil province. The young leaves of 9 tamarix population were collected from natural habitats of this plant in Ardebil province to evaluate the genetic diversity. The leaves DNA were extracted using CTAB method. In this investigation 13 ISSR and 5 SCOT primers were used. A total number of 96 bands were produce by ISSR primers and the highest numbers of bands were amplified by UBC810- UBC826- UBC855. The average percentage polymorphism loci (PPL) was 6.75%, the highest polymorphism with the amount of 100% was in UBC814- UBC826 and UBC855 primers. The dendrogram was drowned based on WARD technique and genotypes classified into four main clusters. Also, a total of 32 band amplified by SCoT primer. The highest and lowest number of bands was obtained from SCoT4 and SCoT1, respectively. The average polymorphism percentage was 75.8%. In general, the results obtained from this study indicate that ISSR and SCoT primers were useful to separate the geographical region of genotypes. These primers also were highly efficient to determine the genetic diversity and relationship of studied tamarix populations. Hence, the use of these primers in other genetic studies like QTL and association mapping are recommended.

Laccases, copper-containing polyphenol oxidases, have numerous biotechnological and industrial applications, especially in industries that require high salt concentrations such as pulp and paper industries, tanneries, and textile industries. In the current study, solid and liquid bacterial culture media containing CuSO4 (3 mM) and guaiacol (0.01% w/v) were used to isolate laccase-producing bacteria from the shore sediments of hypersaline Lake Urmia. Bacteria producing reddish-brown colonies on the culture medium containing guaiacol were considered as laccase-producing strains. Intracellular and extracellular laccase activities were measured spectrophotometrically at 420 nm using ABTS (2,2-azino-di-[3-ethylbenzo-thiazolin-sulphonate]) as a substrate. In this study, four bacterial strains were isolated from the shore sediments of Lake Urmia using a culture medium containing guaiacol, of which only one strain was detected with significant extracellular laccase activity. Microscopic investigation and 16S rRNA gene sequence analysis showed that this strain is a rod-shaped Gram-positive bacterium belonging to the genus Brevibacterium and was named Brevibacterium sp. strain LUBr01. The 16S rRNA sequence of LUBr01 strain is available on the GenBank nucleotide database under the accession number MN588176.1. Due to the proper laccase activity, LUBr01 can be useful for industrial process related to laccase. Also, saline Lake Urmia can act as a source of microorganisms with the ability to produce special enzymes such as laccase that can be used in salinity conditions.

In this experimental study, the gene encoding the protein Chapron biogenesis fimbriae Csu was amplified by carbapenem-resistant Acinetobacter baumannii by PCR. the gene was cloned and subcloned into T and pcDNA3.1 (+) vectors, respectively. Confirmation of gene cloning was evaluated by three PCR, cleavage enzymes, and sequencing. Then, 100 μl of 100 ng/ml concentration of the final recombinant vector pcDNA3.1 (+) - csuC was injected into the quadriceps muscle of BALB / c mice and the expression of the target gene in the quadriceps muscle of mice was examined by RT-PCR and the results of expression differences at significant levels P <0.05, P <0.01, and P <0.001 reported. Observation of 831 bp band after RT-PCR in quadriceps muscle of mice in pcDNA3.1 (+) - csuC group compared to control group (PBS) confirms the expression of csuC gene in quadriceps muscle of mice. The results of the study of changes in gene expression in the target group compared to the control group showed that in the target group the expression of the target gene after 2 days showed a significant increase in expression. This increase in expression reached its highest level by day 30 after injection and the difference in expression was significant. The recombinant pcDNA3.1 (+) - csuC construct made in this study can express the csuC gene of Acinetobacter baumannii in the quadriceps muscle of mice. Also, the pcDNA3.1 (+) - csuC construct has the potential to be considered as a gene vaccine in future research.

Hyperglycemia inhibits cell proliferation and collagen production in wounds and may lead to amputation. Today, using herbs to heal wounds such as diabetic wounds is very important. This project aims to investigate the effect of hydroalcoholic extracts of Falcaria vulgaris, Verbascum spp and Silybum marianum plants on the healing of diabetic wounds and collagen synthesis. 50 male mice (10 non-diabetic and 40 diabetics with Streptozotocin) were used in this study. After anaesthesia and wounding on the backs of mice, the wounds of both sham and control groups were treated with Eucerin and three experimental groups were treated with hydroalcoholic extracts of Falcaria vulgaris, Verbascum spp and Silybum marianum for 5 days. The left and right wounds were treated with 25% and 50% Eucerin-based extracts in the experimental groups, respectively. Macroscopic imaging of mice wounds was performed. ImageJ software was used to measure the wound surface and the wound healing rates were calculated. Chromatography and ELISA test were used to measure the level of hydroxyproline to detect collagen. The results were analyzed by SPSS statistical software. The results showed that the wound closure rate (healing) was significantly increased in the treated groups with a low dose of Sickleweed and mullein extract (25%) and a high dose of cardus marianus extract (50%) compared to the control. The present study results showed that the hydroalcoholic extracts of the three plants Sickleweed, mullein and cardus marianus could be effective in increasing collagen and healing diabetic wounds.

Triple Negative breast cancer is a common cancer in the world that grows in breast tissue. The disease has became the biggest human health problem worldwide. Therefore, identifying the key genes involved in this disease can be useful in the diagnosis, prognosis and treatment. The aim of this study was to identify genes with significant expression differences affecting triple negative breast cancer using RNA sequencing transcriptomic data analysis of breast cancer patients compared to healthy individuals.

Illumina-sequenced transcriptomic data of three breast cancer patients and four healthy individuals were collected from the NCBI and SRA databases, and after controlling the quality of the readings using FastQC software, the sequences were aligned with Reference genome was performed with STAR software. Finally, featureCounts and DESeq2 softwares were used to investigate the differential expression of genes. Volcano graph was used to show genes with significant expression differences.

In this study, 31 genes with differential expression were identified with a significance level of p

Epilepsy is a chronic neurological disorder that lacks an effective treatment, and most patients do not respond to existing medications. It is due to the lack of a molecular mechanism of epilepsy and the incomplete understanding of proteins that are involved that epilepsy is largely unknown. It is therefore important to identify and control the epileptic process so that relevant and effective drug treatments can be administered. This study used the electrical kindling method and the proteomics shotgun technique to analyze the proteomes of rats during epileptogenesis. From the analysis of system biology data, it was determined that the cAMP signaling pathway is involved in epilepsy, as well as changes in the expression of proteins that are upstream and downstream of this secondary messenger in the stages of electrical kindling was observed. There was a significant decrease in expression of somatostatin neuropeptide from upstream proteins and an increase in expression of phospholipase D2 and AMPA glutamate receptor type 2 from downstream proteins during epilepsy. Taking advantage of these data, we can consider a new method to identify the mechanism of epilepsy and introduce new drug targets by identifying changes in the expression of cAMP signaling pathway proteins and their roles in the epilepsy process.