فهرست مطالب

  • Volume:8 Issue: 3, 2007
  • تاریخ انتشار: 1386/08/11
  • تعداد عناوین: 8
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  • Parks D., Shahbazi M., Arthur Ch, Gerri Cannon, Smith G Page 98
    Children in the U.S. suffer from high rates of cavities, dental caries, and other childhood oral diseases. Pediatric oral healthcare in the United States is a very important issue; however, despite funding for pediatric oral healthcare from federal, state, and private organizations it remains an often neglected issue. Expanded preventive care services, strengthened relationships between policymakers, dental care providers and public health professionals are needed to improve the oral healthcare delivery for children.
  • Er, Rasfa M Page 110
    In this study, we sought to investigate the pattern of cellular glycoproteins during yeast-to-filament growth transition in Candida albicans, in vitro. By mean of glycoprotein extraction with Concanavalin A-Sepharose, and Western Blot analysis with peroxidase-labeled lectins (concanavalin A, wheat germ agglutinin), we have characterized two glycoproteins (52 kDa and 42 kDa) whose pattern is increased in the filamentous form of C.albicans. Analysis of subcellular fractions of C.albicans showed that the 52/42 kDa glycoproteins are located in the soluble fraction. in vitro treatment of concanavalin A-Sepharose extracted 52/42 kDa glycoproteins with the peptide N-linked glycosidase F showed that the 52 kDa protein is highly N-glycosylated and mannose O-glycosylated, whereas the 42 kDa is N-glycosylated. Regulation of synthesis and / or glycosylation of the 52/42 kDa glycoproteins could be associated with yeast-to-filament growth transition of C.albicans.
  • Nikandish R., Shahbazi Sh, Zand F., Jamshidi H§ Page 120
    Background
    For 40 years cricoid pressure has been used to prevent regurgitation of gastric contents during induction of anaesthesia. Available studies have highlighted poor techniques regarding cricoid pressure. Patient safety could be challenged by incorrect use of cricoid pressure The aim of this study was to assess the practice and knowledge of cricoid pressure among anesthesiologist attending in the ninth international congress of anesthesia and resuscitation in Iran.
    Materials And Methods
    This cross sectional observational study included 59 anesthesiologists attending the 9th international Iranian congress of anesthesia and resuscitation in 2006. Each subject was asked to apply cricoid pressure on an undressed laryngeal model for one minute. The model was an anatomically correct representation of the human larynx. It was mounted onto the base plate of a kitchen scale. Participants were blinded to the results. Five measurements were obtained from each candidate(0,15,30,45 and 60th second).Participants was also asked to complete a questionnaire containing demographic data and some questions regarding attitude and knowledge of participants regarding Slick’s maneuver.
    Results
    Eighteen participants(30%) applied the target cricoid force (3-4 kg).When considering both correct anatomical position and right range of applied force only 11 participants(19%) were successful. Mean applied force significantly decreased at the end of one minute and mean applied force by women was significantly less than men. There was no statistically significant diffenece between academic staff and other anesthesiologists in application of target force. Only eight participants(13.6%) were aware of the target cricoid force.
    Conclusion
    Application of cricoid pressure by the participant anesthesiologists is poor.Use of simple models for training during refresher courses for practicing anesthesiologist in addition to stressing on the theoretical base may improve the quality of performance of the Sellick`s maneuver.
  • Hajia M., Rahbar M., Hossieni Taghavi A§ Page 127
    Diagnosis of brucellosis is based on the rises of antibody titer in patient sera. It is reported clinicians are frequently complain on some of serology results of brucellosis. In this research, all brucellosis patients who were confirmed clinically and by serological methods are entered to the study. In total, sera of 758 patients of Hamedan teaching hospitals were analyzed with Wright, Coombs and 2Mercapto-Ethanol tests. All these patients were received treatment after final diagnosis. In the studied group 29 (3.82%) and 30 (3.95%) of patients had no antibody rise in Wright and 2ME respectively. One patient had antibody titer less than 1/40 in Coombs test. Specific antibody level higher than 1/160 was observed in 49.31% of patients for the Wright test, while it was 43.46% in patients with antibody titer higher than 1/80 of 2ME test.
    Conclusion
    It seems some of those serology negative patients may involved with brucellosis and possibility of infection should be always considered in those serology negative patients since B. abortus antigen are applied in all serology tests. Analyzed results also indicate application of Coombs test can provide clear understanding for those cases with low antibody rises.
  • Hatam Gh, Khorami Hr, Sahebani N†, Sarkari B§ Page 132
    Introduction
    This study was conducted to evaluate the usefulness of dot enzyme-linked immunosorbent assay (dot ELISA) and plate ELISA, using monoxenically-grown Entamoeba histolytica soluble crude antigen, for detecting antiamoebic antibodies in serum samples from suspected amoebic patients and healthy controls.
    Materials And Methods
    Sera from 18 suspected amoebic patients, 15 healthy subjects and 13 patients with other parasitic diseases (leishmaniasis, hydatidosis, ascariasis and trichostrongyloidiasis) were examined for the presence of specific Entamoeba histolytica antibodies by ELISA and dot-ELISA. Both dot and plate ELISA detected antiamoebic antibodies in 15 (out of 18) suspected amoebic.
    Results
    From 15 healthy controls, two sera were found to be positive by ELISA and one of these two was also positive by dot-ELISA. In both assays, no cross reaction was found with the sera from other parasitic diseases including leishmaniasis, hydatidosis, ascariasis and strongyloidiasis. Sensitivity of the ELISA system was determined as 83.3% (95% CI: 57.7-95.6%) and the specificity was found to be 92.5% (95% CI: 74.2-98.7%). Positive and negative predictive values of the system were 88% and 89% respectively. On the other hand, sensitivity of the dot-ELISA was determined as 83.3% (95% CI: 57.7-95.6%) and the specificity was found to be 96.3% (95% CI: 79-99.8%). Positive and negative predictive values of dot ELISA were 93.7% and 89.6% respectively.
    Conclusion
    While the sensitivity of both assays were equal (83.3%), dot-ELISA was found to be more specific (96.3%) in detecting antiamoebic antibodies compared to Plate ELISA (92.5%). Seeing that the dot ELISA is simpler, rapid, inexpensive, equally sensitive and more specific as compared to plate ELISA, its use in serodiagnosis of amoebiasis can be recommended.
  • Beheshti M., Ghotbi Sh Page 138
    Impetigo is a primary superficial bacterial skin infection, initially vesicular or bullous, and later crusted. It is caused by staphylococcus aureus, Streptococcus pyogenes or both. It is highly contagious and usually treated with systemic antibiotics. Possible complications include cellulitis, lymphangitis, furunculosis, Methicillin-resistant Staphylococcus aureus (MRSA) infection, pigmentary changes with or without scarring and acute post streptococcal glumerulonephritis (PSGN).A concise review of literature is presented here to cover the most important aspects of impetigo
  • Basiri K., Rabani G Page 146
    Background
    Multiple Sclerosis (MS) can presents with a wide variety of clinical manifestations. Because of diversity of symptoms and signs, physicians must be careful in attributing non- typical neurological manifestation to psychiatric disorders.Case report: A 40 years old male patient referred to our clinic with history of gradual onset of left sided visual loss; and paresthesia and numbness on left side of the face and body. He was seen by many neurologists and psychiatrists and was treated with diagnosis of conversion reaction. Neurological examination was normal except for subjective sensory loss. Visual acuity was normal on both eyes. Marcus Gunn sign was negative. Brain Magnetic Resonance Imaging (MRI), Visual Evoked Potentials (VEP), and lumbar puncture confirmed diagnosis of multiple sclerosis.
    Discussion
    Multiple sclerosis may present with atypical symptoms and signs and no objective data may be found in neurological examination. Confusion with psychiatric problems has been mentioned in the literature. Physicians must be careful in attributing such symptoms to psychiatric disorders. Above mentioned case is a notable example of such a problem. He presented with simultaneous onset of loss of vision and hemi sensory loss on the same side, which is an anatomical impossibility from a single lesion and mentioned in neurological textbooks a sign of conversion reaction. Also no objective sign was found in neurological examination (negative Marcus Gunn sign and similar visual acuity in both eyes). Key point that resulted in correct diagnosis was careful sensory examination that revealed organic type of sensory loss (area of sensory loss was lesser than half of the face and body, and shift from painless to pain full area was Para sagittal and not in the midline). We concluded that atypical symptoms and signs must not automatically attribute to conversion reaction and accurate neurological examination is the best way of making correct diagnosis.
  • Mirsharifi R., Pakaneh Ma, Aminian A Page 150
    Emphysematous cholecystitis is an unusual but life-threatening form of acute cholecystitis caused by the presence of gas-forming bacteria in the gallbladder. Here we present a case of fulminant emphysematous cholecystitis developing after endoscopic retrograde cholangiopancreatogram (ERCP).