فهرست مطالب

Anatomical Sciences Journal
Volume:5 Issue: 2, 2007

تاریخ انتشار: 1386/05/11
تعداد عناوین: 10

فارسی
English

انتخاب همه

Isolation Induction of Neural and Glial Differentiation and Evaluating the Expression of Five Self Renewal Genes in Adult Mouse Neural Stem Cells

F. Fathi, A. Jafari Kermani, M.R. Golbar, E. Izadpanah, M.Gh Golmohammadi, S.J. Mowla, A. Asgari Page 81

Purpose
Isolation induction of neural and glial differentiation and evaluating the expression of Nucleostemin ZFX Hoxb-4 Sox-9 & Bmi-1 self renewal genes in adult mouse neural stem cells
Materials And Methods
Breifly for isolation of neural stem cells frontal part of adult mouse brain was minced in PBS and digested by enzyme solution containing hyaloronidase and trypsin. Isolated cells were cultured in medium supplemented by EGF bFGF. After one week primary neurospheres were appeared in culture medium which differentiated into neural and neuroglial cells after transferring to poly-L-ornyethin coated dishes. Differentiated cells were examined by morphological immunocytochemical and molecular evaluations.
Results
Our results showed that isolated cells from periventricular area of mouse adult brain can be expanded in medium containing EGF bFGF and Fetal calf serum and differentiated into neural and neuroglial cells. Immunocytochemical studies for GFAP MAP2 and O4 genes proved the differentiation. Our results also showed that Nucleostemin ZFX Hoxb-4 Sox-9 & Bmi-1 are expressed in mouse neural stem cells.
Conclusion
Mouse adult brain contains a type of stem cell which can be differentiated into neural and neuroglial cells. In addition expression of Sox-9 Nucleostemin and Bmi-1 self renewal genes in undifferentiated neural stem cells can be considered as a proof for stem cell identity of isolated cells. This study is the first report which shows the expression of ZFX and Hoxb-4 genes in neural stem cells.
Keywords: Neural stem cells Self renewal Neural cells Neuroglial cells

Abstract View Paper Original: Persian
Central Neuropathic Pain after Graft of Bone Marrow Stromal Cells in the Spinal Cord Contusion of Rat

V. Pirhajati Mahabadi, T. Tiraihi, A.R. Khalatbary, A.R. Aziz, Zade Delshad Page 93

Purpose
The purpose of this study was the investigation of thermal and mechanical Allodynia after BMSCs grafting in the Spinal Cord Contusion of rat
Materials And Methods
In this study used 40 female Sprague- Dawley 6-8 week old that 33 rats received vertebral laminectomy to expose spinal cord (L1 vertebral level). The cord was then contused with the weight drop device. Experimental groups consist group 1(Intact) rats without intervention (n=7); group 2 and 3: 16 rats given PBS alone without cell administration respectively 10 rats intravenously (vehicle1) and 6 rats intraspinally (vehicle2); group 4 and 5: 17 rats given BMSCs after 4 passages respectively 10 rats intravenously and 7 rats intraspinally. Grafts were done 1 week after injury. Behavioral tests were done for 10 weeks (after injury). For thermal testing rats were placed on a hotplate. Mechanical sensitivity was assessed with Von Frey Hairs. Tracing of the cells at the injured site was performed immunohistochemically and then astrocyte differentiation was detected with the double-Staining method.
Results
BMSCs-engrafted rats showed significant decrease in withdrawal threshold and time latency to licking of hindpaws in comparison with vehicle groups. Also there was astrocyte differentiation.
Conclusion
The results demonstrate that intraveous and intraspinal grafts of BMSCs in the cases of spinal cord contusion enhance thermal and mechanical Allodynia. Also Allodynia could be positively correlated with the number of astrocytes.
Keywords: Bone Marrow Stromal Cells (BMSCs) Graft Spinal Cord Contusion Central neuropathic pain Allodynia

Abstract View Paper Original: Persian
Evaluation of Behavioral Effects of Trans-Resveratrol in the Hemi- Parkinsonian Rat Model

A. Faghihi, M.T. Joghataie, S. Darabi, M. Mehdizadeh, M. Roghani, M. Bakhtiari Page 107

Purpose
The motor control deficits exhibited with Parkinson’s disease (PD) are particularly well documented for sequencing tasks. The purpose of this study was to evaluate the neuroprotective potential of trans-resveratrol administration in an experimental model of PD.
Materials And Methods
1 week before (baseline) the surgery rats were tested with systemic apomorphine (0.5 mg/kg i.p.) to evaluate the behavioral effect of the nigrostriatal lesion (turning behavior). The number of full rotations performed by the animals was registered at 10-min intervals for 60 min starting 1 min after apomorphine injection. The same procedure was repeated after 14 days post lesioning by 6-OHDA in the left striatum. Rotational response was expressed as the number of net full (360°) turns per hour. The number of contralateral rotations was counted as positive scores for apomorphine. Net number of rotations was defined as the positive scores minus the negative scores.
Results
There were no significant differences among the groups at baseline (before surgery). Statistical analysis of the total net number of rotations made over a 60-min period 2 weeks after the surgery showed that apomorphine caused a very significant contralateral turning in the rats of the L group (P
Keywords: trans, Resveratrol 6, hydroxydopamine Parkinson's disease Rat

Abstract View Paper Original: Persian
The Effects of Low-Level Helium-Neon Laser Therapy on Healing of Partial Thickness Defect of Experimental Induced Diabetic Rats

Sh Abdi, M. Bayat, F. Javadieh, X. Mohsenifar, G. Torkaman, Y. Sadeghi, F. Rezaei Page 115

Purpose

The aim of present study was to study the effects of low-level helium –neon laser irradiation on biomechanical properties of tibiae partial bone defect in streptozotocin –diabetic rat.

Materials And Methods

Twenty male rats were used. Diabetes was induced in rats by one time intraperitoneal injection of 60 mg/kg streptozotocin. After one month under sterile conditions and general anesthesia one partial bone defect were made in mid portion of right tibia of rats. Rats were divided into control and no 1 and no 2 experimental groups. Rats of no 1 and 2 experimental groups were received he-ne laser with 2.1 J/cm² and 11.6 J/cm² energy densities three times a week for six weeks. After that rats were killed and their tibiae were extracted and were examined by three point bending biomechanical test. Data were analysed by ANOVA and expressed as mean±SEM.

Results

The energy absorption (N/mm) of no1 experimental group control and no2 experimental groups were 52±45 44.2±35.2and 36.3±20.1 respectively. Load at break (Nmm) of no1 and no2 experimental group and control group were 34.2 ±13.2 32.6± 18.6 and 31.8± 27.4 respectively. Maximum force per square mm of no1 experimental group control and no2 experimental group were 8.2± 3.57 8.2 ±4.4 and 7± 4.96 respectively. There were not significant differences between all above mentioned parameters and also elastic stiffness maximum force and bone surface area of groups.

Conclusion

He-Ne laser irradiation on tibias of partial bone defect in streptozotocin diabetic rats did not cause positive effects on fracture healing from biomechanical evaluation point of view.

Keywords: Low, Level Laser therapy Diabetes Bone repair

Abstract View Paper Original: Persian
Efficiency of Ovine Fibroblast or Cumulus Cells for Somatic Cell Nuclear Transfer in Sheep

M.H. Nasr Esfahani, F. Molavi, M. Hosseini, M. Frozanfar Page 125

Purpose
Despite remarkable progresses have been achieved in the field of somatic cell nuclear transfer (SCNT) there is little information regarding the effect of donor cell type on the efficiency mammalian somatic cell cloning in vitro. This study compared in vitro developmental competency of sheep enucleated oocytes reconstructed with either fibroblast or cumulus cells.
Material And Methods
Adult fibroblast cells were taken from skin biopsies of one male and one female adult sheep. Cumulus cells were harvested from the same female sheep. Direct whole cell injection procedure was used for embryo reconstitution. Activated reconstructs were cultured in TCM+10%FCS medium up to days 7 post activation whereas in vitro fertilized oocytes served as control.
Results
Although the rate of day 7 blastocyst formation in cumulus donor cells (19.7%) were greater than the male and female fibroblast donor cells (17.5% and 15.7% respectively) these differences were not significant. Control group on the other hand induced significantly greater blastocyst rate (29.7%) rather than all the three treatment groups.
Conclusion
Both of cumulus and fibroblast can be used as donor cell for ovine somatic cell nuclear transfer. Since no significant difference was observed in term of blastocyst rates between the three SCNT groups therefore concluding donor cell type has no significant effect on the overall efficiency of in vitro production of cloned sheep blastocyst.
Keywords: Ovine Somatic cell nuclear transfer Cumulus Fibroblast

Abstract View Paper Original: Persian
The Effect of Granulosa Cells Co-culture and Retinoic Acid on Maturation and Development of Immature Mouse Oocytes In Vitro

H. Eimani_L. S Tahaei_K. Parivar_M. Rezazadeh_S. Kazemi_A. Shahverdi_P. Eftekhari Page 137

Purpose
The purpose of this study was to develop an appropriate medium for in vitro maturation (IVM) of immature mouse oocytes.
Materials And Methods
Germinal vesicle of female NMRI mouse oocytes (6-8 weeks old) were collected from ovaries and cultured in maturation medium MEM-α supplemented with: 100 mlIU/ml rFSH + 7.5 IU/ml hCG + 5% FCS (Control group) and 2 μM all- trans retinoic acid (t-RA) in presence or absent of granulose cells. Ethanol (Sham group) 0.2% (v/v) used as solvent. After 24 hours the matured oocytes were fertilized with spermatozoa in T6 medium and cultured for 5 days. Cultured immature oocytes development to the morula and blastocyst stages was studied.
Results
The retinoic acid supported progression and resumption of meiosis and also increased advancing the oocytes to Metaphase II formation of morula and blastocyst compared to control group. When there were not 2 μM t-RA and granulose cells in IVM medium significantly lower maturation rates were observed followed by a decrease in the percentage of embryos reaching the blastocyst stage. Whereas when 2 μM t- RA and granulose cells monolayer were present in the IVM medium better results in comparison with control group were obtained.
Conclusion
Results attest that co-culture of granulose cells with 2 μM all-trance retinoic acid during in vitro maturation enhanced mouse oocytes maturation and improved embryonic development.
Keywords: Granulose cells Retinoic acid In vitro maturation Mouse Oocytes

Abstract View Paper Original: Persian
Valproic Acid-induced Skeletal Defects and Spina Bifida in Rat Fetuses

M.R. Nikravesh, M. Jalali Page 147

Purpose
Among antiepileptic drugs valproic acid (VA) is a well known teratogenic agent. We previously reported that maternal administration of VA during critical periods of rat pregnancy can produce very rare CNS defect syringomyelia. The purpose of the present study was whether VA can affect other organs based on this finding we studied the teratogenic effects of it on rat developing skeletal system.
Materials And Methods
Based on this experimental study 3 experimental groups of pregnant wistar rats were received single dose of 800 mg/kg VA on gestational days 9 -11 respectively. 3 Control groups were received the same volume of physiological serum at the same periods. On day 18th of gestation all pregnant rats were anesthetized and their fetuses were examined and histochemical study around their vertebral region were carried out.
Results
Our finding indicated different kinds of malformations in experimental groups which was consist of growth retardation embryonic absorbtion as well as skeletal system defect by special regard in vertebral column. These data revealed that the rate of spina bifida and vertebral abnormal flexions in experimental group 1 increased significantly in comparison experimental groups 2 and 3.
Conclusion
These findings suggest that day 9th of rat pregnancy is critical period for vertebral column development and during this period of time VA or its metabolites may be interfere with developmental phenomena which can produce vertebral defects such as spina bifida.
Keywords: Valproic acid Malformation Skeletal defects Spina bifida Rat

Abstract View Paper Original: Persian

علمی مصوب

Anatomical Sciences Journal

مجله علوم تشریح ایران

دوفصلنامه پزشکی به زبان انگلیسی

آخرین شماره | آرشیو

ISSN: 1728-6158

از سال 2013 بصورت انگلیسی منتشر می شود.

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انجمن علوم تشریح ایران

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دکتر مجتبی رضازاده ولوجردی

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فهرست مطالب

Anatomical Sciences Journal Volume:5 Issue: 2, 2007

Anatomical Sciences Journal

مجله علوم تشریح ایران

Anatomical Sciences Journal
Volume:5 Issue: 2, 2007