فهرست مطالب

فصلنامه علوم پزشکی دانشگاه آزاد اسلامی
سال هفدهم شماره 4 (پیاپی 50، زمستان 1386)

76 صفحه، بهای روی جلد: 10,000ريال
تاریخ انتشار: 1386/12/20
تعداد عناوین: 10

فارسی
English

انتخاب همه

Evaluation the immunogenesity structure of

Fatemeh Rouhpllah, Ghader Khalili, Soroush Sardari, Masoumeh Tavassoti Kheiri, Aref Amirkhani, Taher Nejadsattari, Fereidoun Mahboudi Page 177

Background
HIV recombinant proteins can be used as immunogen and inducer of immune system. The gag and env proteins are among the most important ones that are located on internal and surface sections, respectively. In this study, we considered the immunogenisity structure of HIV recombinant protein gp41-p24, which has not yet been studied in detail before.
Materials And Methods
In the present study, the HIV recombinant protein gp41-p24 was prepared by cloning methods and kept as unfolded. Using the dilution procedure, unfolded protein was changed to refolded state. The molecular weight and concentration of protein (refolded and unfolded) was measured by electrophoresis and spectrophotometer methods. The measurement of refolded protein can be estimated by native gel and circular dichroism method (CD) based on secondary structure of the protein. Immunological activity and immunogenic structure of these two proteins, based on protein type and optical density was recorded by ELISA and western blot methods.
Results
Our results showed that molecular weight of each protein was 32 KD and also they were pure. The refolded protein was observed by native gel method. In the above protein compared with the unfolded one, increased content of helix and beta strand structures and decreased random form was shown. Immune reaction with the antibodies in the serum of HIV positive control patients was observed in the standard and refolded proteins. There was no significant difference based on the protein type.
Conclusion
Our research indicated that the HIV recombinant protein gp41-p24, after refolding, has immunogenic activity and we suggest its application as an immunogen in immunization and stimulation of immune system.

Abstract View Paper Original: Persian
comparison between the results of fissurectomy and lateral internal sphincterotomy in the management of chronic anal fissure

Seyedreza Mousavi, Mehrzad Sharifi, Pegman Kharazmi, Mohammad Zeynal Zadeh, Ali Kaviani, Mikaeil Rezayi, Arash Najafbeygi, Alireza Saberi Ghouchani Page 183

Background
Although lateral internal sphincterotomy is an effective treatment of chronic fissure in ano, it may lead to serious complications, of these, incontinence to flatus and fecal soiling are the most distressing. To avoid such complications, we proposed fissurectomy as an alternative surgical treatment.
Materials And Methods
Totally, 62 consecutive patients through the sequential sampling were divided into two groups. 30 patients underwent fissurectomy (F) and 32 underwent lateral internal sphincterotomy (LIS). After a median follow-up of 22 months, we compared the results of the two procedures. In addition to frequent visits on a predetermined basis, a telephone inquiry into fissure recurrence and continence status was made.
Results
All patients in either group were pain free and without bleeding within one week. In both groups urinary retention was noted in one patient. Incontinence to flatus was in the LIS group in two (6.2%) patients but no incontinence was noted in the F group. There was one patient (3.1%) with fissure recurrence in the LIS group but no one in the F group. No patient in either group afflicted with anal stenosis or perianal infections. All wounds healed within 8 weeks. 29 patients (96.6%) in the F group and 28(87.5%) in the LIS group reported satisfactory results with their procedure.
Conclusion
In the surgical treatment of chronic anal fissure not responding to conservative management, fissurectomy may be a sphincter-sparing alternative and perhaps preferable surgical technique with less total complications.

Abstract View Paper Original: Persian
Investigate the effect of regular exercise on diabetic nephropathy in rat

Yousof Doustar *, Iraj Salehi, Mostafa Mohammadi, Daryoosh Mohajeri, Mehrdad Hashemi Page 187

Background
Diabetes mellitus and its complications are a public health problem. Exercise is frequently recommended in the type І and type П diabetes mellitus and can improve glucose uptake by increasing insulin sensitivity and lowering body adiposity. Diabetic nephropathy has become the main cause of renal failure. The purpose of this study was to investigate the effect of regular exercise on diabetic nephropathy.
Materials And Methods
In this study, fifty-six 12-week rats weighted 200-300 gr were selected and assigned into two groups (treatment and control). Diabetes mellitus was induced by intraperitoneal injection of streptozotocin (50 mg/kg). The treatment group were kept in normal conditions of food and place with regular exercise (treadmill) for 12 weeks, 5 days in a week, an hour every day. In control group we prepared normal conditions (food and place) without any physical activity/regular exercise. After 12 weeks renal tissues were sampled in both groups and 5-6 micron tissue section were prepared through H&E staining method.
Results
Histopathological analysis of tissue section in control group demonstrated that glumerosclerosis, artheriolosclerosis, perivascular cuffing of mononuclear inflammatory cells, tubular cells nephrosis and hyaline cast in lumen of renal tubules and a little pathological changes in treatment group were observed. Mean deference of histopathological changes and proteinuria in two groups were significant.
Conclusion
We demonstrated that regular exercise (treadmill) is able to reduce pathological changes and improve diabetic nephropathy disease in diabetic patients resulting in the reduction of HbA1c, oxidative stress, hyperglycemia, VLDL, expression of apoptosis regulatory

Abstract View Paper Original: Persian
Chondro-protective effects of pomegranate on experimental osteoarthritis

Mahsa Hadipourjahromi *, Reza Mozaffari Kermani, Maryam Kadivar, Marta Safavi Page 199

Background
To study the effectiveness of pomegranate juice (PJ) on osteoarthritis (OA), we used mono-iodoacetate (MIA), an inhibitor of glycolysis, in tibiofemoral joint of mice that promotes loss of articular cartilage similar to that noted OA. Pomegranate juice (PJ) is increasing in popularity because of its high antioxidant content, already known to help prevent heart disease. However, no histopathological studies have undertaken in vivo to investigate whether PJ protect articular cartilage.
Material And Methods
We described the histopathology in the subchondral bone and cartilage of mice knee joint treated with a single intra-articular injection of MIA (0.1 mg) and sacrificed at 1, 14 and 28 days post injection. Then, the beneficial effect of oral PJ was studied in different groups group 1: administration of PJ (4ml/kg), group 2: administration of PJ (10ml/kg) and group 3: administration of PJ (20ml/kg). Histopathological changes in knee joints were studied after two weeks.
Results
Histologically, the early OA was characterized by areas of chondrocytes degeneration/ necrosis sometimes involving the entire thickness of the articular cartilage in the tibial plateaus and femoral condyles. Changes to the subchondral bone and proteoglycane contents, were observed and also, there was focal fragmentation and collapse of bony trabeculae with fibrosis and necrosis. Synovial cell proliferation was noted. Interestingly, administration of PJ in different group of mice prevented the negative effects of iodoacetate, in a dose dependent manner. Chondrocyte damages were significantly prevented and proteoglycane were less affected, especially in group receiving high amount of PJ and no cell proliferation and inflammatory cell were detected in synovium.
Conclusion
Fast and progressive damage to articular cartilage is induced by single intra-articular injection of MIA, which mimic exactly human OA. In this study, the effectiveness of PJ in improvement of histopathological damages is emphasized and its chondroprotective effects in vivo are highlighted.

Abstract View Paper Original: Persian
Effects of progressive muscular relaxation training on anxiety level and health related quality of life in anxious patients after coronary artery bypass surgery

Tahereh Dehdari, Alireza Heidarnia, Ali Ramazankhani, Saeed Sadeghian, Fazlollah Ghofranipour, Gholamreza Babaei Rouchi, Sorayya Etemadi Page 205

Background

There is a lack of clinical trials studying the effects of relaxation techniques as a basis of stress management on anxiety and quality of life in anxious patients after heart surgery. The purpose of this study was to investigate the effect of progressive muscular relaxation training on reducing anxiety and improving quality of life among patients with high levels of anxiety after coronary artery bypass graft surgery (CABG).

Material And Methods

A randomized controlled trial with a prospective pretest-posttest experimental design was used. One hundred ten patients with threatening anxiety level, 6 weeks after coronary artery bypass surgery were randomly assigned into experimental and control groups. The experimental group received progressive muscular relaxation (PMR) training, and the control group received only routine cardiac rehabilitation care. State/trait anxiety was measured with the Spielberger State-Trait Anxiety Inventory, and quality of life was measured with the SF-36 questionnaire.

Results

There were significant reductions in state anxiety) P=0.001) and trait anxiety) P=0.001) in the experimental group when compared with the controls after the intervention. Significant improvements were found in the following quality of life measures after participating in progressive muscular relaxation training: physical functioning) P=0.00001), role-physical (P=0.03), bodily pain)P=0.00001), general health) P=0.00001), vitality) P=0.00001), social functioning) P=0.001), role-emotional (P=0.00001) and mental health) P=0.00001).

Conclusion

Teaching relaxation techniques could serve as a resource for improving heart patients’ psychological health and quality of life.

Abstract View Paper Original: Persian
Prevalence of different types of allergy among textile workers in Kashan

Ahmad Masoud, Hamid Shekarriz Page 219

Background
Occupational allergy is one of the most important issues in the field of occupational health and is one of the major problems among workers in factories with exposure to allergens. The present study was conducted to survey the frequency of different types of allergy and allergens among textile workers in Kashan City.
Materials And Methods
This cross sectional study was performed among 437 randomly selected workers of 7 textile factories in Kashan City during 2006.
Results
Respiratory (36.3%) and skin (27.1%) allergies were the most common type of occupational allergies among workers. Smoking, type of textiles in exposure, and the state of machines (ne or used) were associated with type and location of allergy.
Conclusion
Safer occupational environments and better quality of ventilation and using workers with no history of allergic diseases and smoking may have a prompt effect in reduction of occupational allergy.

Abstract View Paper Original: Persian
Efficacy of thought aversion technique on

Shahla Mollahosseini *, Faezeh Sahbaei, Ebrahim Ebrahimi, Zeynab Sadighi Page 223

Background
Applying pain reduction techniques is an essential right for all patients. The present study was conducted to determine the efficacy of thought aversion technique on pain severity of patients with acute abdomen.
Materials And Methods
For this clinical trial, 50 patients admitted to the surgical unit of hospitals affiliated to Mashad University of Medical Sciences were consecutively selected. Initial data were gathered by a questionnaire.
Results
Totally, 62% of patients had experienced sever pain before our intervention, of whom, 58% described moderate pain following the thought aversion technique. The mean pain score was reduced from 7.88 to 4.38 following the intervention. Patients admitted for herniorraphy took benefit much more than the other groups (mean 4.14±0.90), however, this technique was not associated with satisfactory out come in patients underwent laparoscopic cholecystectomy.
Conclusion
Thought aversion technique is associated with satisfactory results in pain reduction of patients undergoing surgery.

Abstract View Paper Original: Persian
A case of endocervical microglandular hyperplasia which was formerly diagnosed as adenocarcinoma

Minoo Saatian *, Fatemeh Mohammadyari Page 227

Background
Endocervical microglandular hyperplasia is known as benign proliferation of endocervical glands. Florid form may be mistaken for adenocarcinoma. The patient is a 27-year old female referring when she was during her 37th week of pregnancy complaining of decreased movements of her fetus. In vaginal examination a big polypoid and fragile mass was obvious in cervix. The pathological report of the mass was adenocarcinoma. The patient refused further treatment. She safely delivered and consulted her physician 40 days after delivery. In vaginal examination, cervix was completely normal with no mass. Colposcopy and several biopsies from different points of cervix were performed and all reports were endocervical microglandular hyperplasia. She has been followed for 2 years after her delivery and fortunately she has no problem until now.

Abstract View Paper Original: Persian

علمی مصوب

فصلنامه علوم پزشکی دانشگاه آزاد اسلامی

Medical Science Journal of Islamic Azad Univesity Tehran Medical Branch

فصلنامه پزشکی

آخرین شماره | آرشیو

ISSN: 1023-5922

صاحب امتیاز:

دانشگاه آزاد اسلامی واحد علوم پزشکی تهران

مدیر مسئول و سردبیر:

دکتر سید حسین یحیوی

تلفن نشریه: ۰۲۱-۲۲۶۱۸۵۵۱

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فهرست مطالب

فصلنامه علوم پزشکی دانشگاه آزاد اسلامی سال هفدهم شماره 4 (پیاپی 50، زمستان 1386)

فصلنامه علوم پزشکی دانشگاه آزاد اسلامی

Medical Science Journal of Islamic Azad Univesity Tehran Medical Branch

فصلنامه علوم پزشکی دانشگاه آزاد اسلامی
سال هفدهم شماره 4 (پیاپی 50، زمستان 1386)