فهرست مطالب

Jundishapur Journal of Microbiology
Volume:1 Issue: 1, Oct-Dec 2008

  • تاریخ انتشار: 1387/08/11
  • تعداد عناوین: 10
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  • Mohammad Roayaei, Hamid Galehdari Page 1
    Thermostable DNA polymerase gene from Thermus aquaticus was cloned into constructedTaq from Thermus a Qaticus (pTTQ) plasmid using EcoRI and SalI sites with subsequenttransformation in Escherichia coli strain (TOP10). The use of Isopropyl-β-Dthiogalactopyranosid(IPTG) as inducer of interested gene expression under control of thelac promoter was investigated. The optimization of enzyme induction by IPTG wasdetermined at shake flask level to be 0.52mM at exponential growth phase. Enzymepreparation was performed by lysis the cultured cells. Afterwards, the cell suspension wasincubated at 75°C to denature all heat sensitive proteins in the cell suspension that havebeen removed by subsequent centrifugation. Finally, the clarified supernatant containingheat resistant Taq DNA polymerase was collected and stored at -80°C. The activity ofenzyme was compared with commercial Taq DNA polymerase, which remained whenstored in buffer containing 50% glycerol, at -20°C. The purified enzyme had a molecularweight of 94 KDa, as estimated by SDS-PAGE and yielded appropriate enzyme activitycomparing to the commercial Taq DNA polymerase.
  • Seyed Mohammad Alavi Page 6
    Frequency of infections in Injecting Drug Users (IDU) varies in different areasaccording to socioeconomic and geographic situation as well as HumanImmunodeficiency Virus (HIV) infection rate. The present study was performed todetermine the frequency of infections in IDU-HIV positive patients. Forty known casesof HIV infected (by positive ELISA and western blot tests) were investigated for variousinfections in Razi hospital in Ahvaz, South West of Iran during 2001–2003. Medicalcharts including age, duration of addiction, imprisonment, site of infection, signs andsymptoms, laboratory findings, imaging result and echocardiography report wereprepared. Data were analyzed in SPSS software by using descriptive statistical methods.Forty IDU-HIV positive male patients with a mean age of 25±6.3 years were included inthis study. More than 50% of patients had a history of imprisonment. Bacterial infectiondiagnosed in 40% and viral infection in 100%. Three patients died due to mixedinfection and AIDS. The obtained rate of infections in IDU-HIV positive in Ahvaz wasvery higher than expected. Rapid diagnosis of above mentioned infections, appropriatemanagement and treatment can prevent or postpone progressing of AIDS.
  • Mahmoud Rahdar, Sharif Maraghi, Abdullah Rafei Mhommad Razijalali Page 10
    In this study, we collected 100 samples of liver hydatid cyst and blood of infected sheepfrom the Ahvaz abattoir. The existing Ca, P, Mg, Na and K were measured by an autoanalyzer apparatus. The result of T. student test showed that there are a significantdifference between serum and fluid of cyst in all electrolytes (p<0.05). There was also asignificant difference between these electrolytes in infected sheep and normal sheep(p<0.05). These results confirmed that the entrance of essential electrolytes havedepended on selective permeability and parasite requirement. Ca and P have vital rolesin the prevention of hydatid cyst fluid acidity. Understanding parasite nutritionbehavior would help us to conduct a better drug treatment in inoperative cyst viaselection of effective drug and adherence of this drug to biological material that promotedistribution of drug to the cyst.
  • Mostafa Feghhi, Eskandar Hajiani, Gholamreza Khataminia Page 15
    Helicobacter pylori infection is one of the most common infections worldwide. Centralserous chorioretinopathy (CSCR) is a serous macular detachment that usually affectsyoung people. The aetiopathogenesis of the disease is still not completely understood.Recently, an interesting association has been observed between this disease and the H.pylori infection. This study was conducted to investigate a possible association betweenH. pylori infection and CSCR. A prospective study was performed and we evaluated atotal of 54 CSCR patients (48 males and 6 females, median age 35.7 years), and a controlgroup of 59 patients (25 women, 34 males; mean age 42.6±11 years) who referred togastroenterology department of Imam Hospital, Ahvaz Jundishapur University ofMedical Sciences, were studied. Central serous chorioretinopathy was diagnosed on thebasis of findings in ophthalmic examinations and confirmed by fluorescein angiogram.All patients underwent a 13C-urea breath test (UBT) and serum IgG anti-bodies to H.pylori by enzyme-linked immunosorbent assay technique to detect H. pylori infection.Patients were defined as H. pylori infected if both tests were positive. The mean durationof symptoms before diagnosis was 10.5±4.5 days. Overall no statistically significantdifference was found between left and right eyes, bilaterally was in 5 patients (9.2%).The incidence of H. pylori infection was 68.5% in CSCR patients and 65% in controlsubjects (p=0.64). These results indicate that the prevalence of H. pylori infection is nothigher in patients with CSCR than in controls. Further large studies will be required todetermine the role of H. pylori infection in patients with CSCR.
  • Ali Zarei Mahmoudabadi, Majid Zarrin Page 20
    Fungi found in soil and the variation and prevalence in each area depends onenvironmental and nutritional conditions. Considering the importance of soil intransmission of diseases, this study aims at assessing the frequency of dermatophytesand related keratinophilic fungi, potentially pathogenic fungi in various samplescollected from two public parks in Ahvaz, SW Iran. Five hundred samples werecollected from different parts of the Zoo and Laleh parks in Ahvaz. The fungal flora wasanalyzed in different samples for the presence of dermatophytes and keratinophilicfungi by hair baiting technique. Specimens were contained soil, carpet pad techniquefrom the walls of the cages, and animals’ residues. The samples were cultured onSabouraud''s dextrose agar, containing chloramphenicol and cyclohexamide, andincubated at 25-27οC for 4-5 weeks aerobically. Isolates were identified by colonymorphology, slide cultures, and differentiation tests. Most of the isolated dermatophyteswere collected from the soil of the Zoo park. The most common keratinophilic funguswas Chrysosporium (5). Other species were Trichophyton mentagrophytes (3), T.verrucosum (2), T. schoenleinii (1) and M. gypseum (1). The results demonstrated thatdermatophytes and related keratinophilic fungi of Ahvaz is somewhat different fromthat in other parts of Iran. This may be due to the different climatic conditionsprevailing. Considering, the fact that most of the potential pathogenic fungi wereisolated from the Zoo park, where there are wilds and domestic animals.
  • Ahmad Taghavi Moghadam, Koroush Afsharpad Page 24
    The present study shows the production of diphtheria toxoid in fermentor as comparedto old static method of production. With using 45l glass fermentor which has theprovision for growth of the organisms under controlled conditions with regard totemperature, pH, aeration and addition of maltose which can be automaticallymonitored and maintain microorganism growth toxin production and recording all ofthe conditions during growth. We succeeded to produce diphtheria toxin with 115 Lf(Rammon flucolation titer) within 40h time period in a minimum possible space, thenthe toxin was detoxified with formaldehyde, concentrated and purified and finallyquality controlled and compared with toxin produced with static method. Fermentorproduction showed a remarkable increase in volume, titer and immunogenecity in ashorter period of time, lower contamination and minimum labor work andrequirements. Therefore this technique can be industrially used for production ofdiphtheria Toxin (DT) and Diphtheria Pertussis Toxin (DPT) vaccines.
  • Babak Vazirianzadeh, Setareh Shams Solary, Mahmoud Rahdar. Reza Hajhossien, Manijeh Mehdinejad Page 28
    The house-fly, Musca domestica L., not only is a nuisance pest, but also acts as animportant mechanical vector for lots of pathogenic microorganism agents, including:bacteria, protozoa, worms, fungi and viruses amongst humans and animals. The aim ofthis study was to isolate and identification bacteria that are pick up by house-fly overthe human and animal premises. In this study totally 230 houseflies were collected toisolate their bacteria, from the central slaughter house and a zoo in Ahvaz SW Iran. Themost common isolated bacteria were Escherichia coli with 230 cases (36.5%). The resultsof the current study confirm that flies are much more than a nuisance and that they posepotentially serious health risks. Consequently, the population of houseflies has to becontrolled.
  • Seyed Mohammad Alavi, Mohammad Hassan Pipelzadeh Page 32
    Sacroilitis is an inflammatory disease, which is often caused by Brucella species (mainlyby Brucella melitensis, and B. abortus), Mycobaterium tuberculosis or inflammatorydiseases such as ankylosing spondilitis. It is rarely associated with Staphylococcalinfections. In this report, a case of a 24-year-old sailor with a history of low back pain ispresented who was initially suspected to suffer from ankylosing spondilitis orbrucellosis. Further evaluation of this case revealed septic sacroilitis due toStaphylococcus aureus and was successfully treated with vancomycin.
  • Ali Zarei Mahmoudabadi, Reza Yaghoobi Page 35
    In the present cases we describe two normal patients who developed extensive tineacorporis that was complicated by multiple subcutaneous papules and pustule caused byTrichophyton rubrum. This form of dermatophytosis has only rarely been described.Two 18 and 20-year-old men were examined for tinea corporis. Direct KOHpreparations from skin scrapings showed septate branching mycelium andarthroconidia in both patients. T. rubrum was also identified in cultures.
  • Mohammad Omidian, Mohammad Ali Mapar, Zahra Omidian Page 38
    A 45-year Iranian man referred to the clinic with a severe itching and multiple papularlesions on his trunk, buttocks and extremities. Symptoms and signs of lesions simulatedthe clinical features of mycosis fungoides (Cutaneous T cell lymphoma). There were (notany) no typical features of leprosy except a localized patch of numbness on the righthand. Slit skin smear and histopathological slide showed features of lepromatousleprosy. Clinicians practicing in leprosy endemic areas should keep lepromatous leprosyin mind while investigating patients with varied and multiple lesions.