Anatomical Sciences Journal
Volume:7 Issue: 2, 2010

The assessment of the ability of combined treatment of bone marrow stromal cells graft (BMSCs) and oral administration of Coenzyme (CoQ10) in rat model of Parkinson disease as a good substitute for common current Parkinson treatments and the comparison of this combined treatment method with alone application of these treatments

In this experimental study of male Wistar rats were used. They were divided into six groups: control sham lesion treatment groups with oral administration of CoQ10 treatment with graft BMSC and combined treatment with graft BMSC and oral administration of CoQ10. Oral administration of CoQ10 with 200 mg/kg/daily dose started a week before the model creation procedure and continued throughout the whole treatment period. The laboratory model of Parkinson disease in rats was performed by injecting 2.5 microlitre saline solution 0.9 % containing 8 micrograms 6-hydroxy dopamine (6-OHDA) and 0.2 % ascorbic acid in substantia nigra pars compacta. Also in sham group the same volume solution saline-ascorbic was injected. BMS Cells were labeled by 5-Bromo-2´-deoxyuridine (Brdu) before transplantation. Behavioral assessment before creating the model two weeks after creating the model and eight weeks after cell transplantation was performed. At the end of second month of treatment Immunohistochemistry and histology Studies were performed.

Behavioral assessment of two groups of alone treatments indicated the equal recovery in comparison with lesion group (p

Staphylococcal enterotoxin B (SEB) is a potent inducer of cytotoxic T-cell activity cytokine production and necrosis induction in vivo. Monophosphoryl lipid A (MPL) is an adjuvant derived from the lipopolysaccharide of E.coli Salmonella Minnesota Re595 and other gram negative bacteria.

In this research The antitumor and antimetastatic effect of intra-venus injection of Monophosphoryl Lipid A (MPL) staphylococcal enterotoxin B (SEB) and SEB+ MPL was evaluated using Balb/C male mice bearing inoculable mice Fibrosarcoma. The anti tumor effect of SEB+ MPL SEB and MPL in mice with inoculated fibrosarchoma tumor (Wehi-164) was examined by IV injection and the sizes of the inoculated tumors were determined. The inoculated tumors were also examined histologically. Moreover histophatologic study in lung tissue didnt showed any metastasis.

In the mice IV injected group with SEB+ MPL reduction of tumor size show a significant difference compared with mice in the SEB and MPL injected and negative control group. A significantly higher frequency of necrosis in tumor tissues was also observed in mice in the IV (SEB+ MPL)-injected group in comparison with other group. Moreover histophatologic study in lung tissue didnt show any metastasis

Our findings suggest that tumor cell death and the prevention of metastasis be caused by increased Cytotoxic T-cell activity in response to IV injection of SEB+ MPL that need to more investigation.

Animal modeling is a crucial necessity in clinical studies of liver diseases. Authenticity of the data which are produced using this tool under different conditions and accuracy of the analyses and assessments which would be based on such data sets is completely dependent on the adoption of a standardized methodology for analyzes and assessment of these data sets. Cirrhosis and Fibrosis are among the most important diseases which are studied by animals modeling due to the fact that their final structure is usually similar among a verity of patients and also because they are the common end stage of most chronic liver diseases. Up to now different approaches such as hepatotoxicity and surgical methods have been utilized to obtain cirrhotic or fibrotic models that either of which has its especial advantages and disadvantages. It is obvious that using models could indicate the production and treatment mechanisms of disease which we elucidate Fibrosis and cirrhosis here. Considering several animal models which were used for liver disease in the world in this survey we try to explain why what and how an animal must be choosen for modeling and how could be evaluated.