نشریه میکروبیولوژی دامپزشکی
سال ششم شماره 1 (پیاپی 19، بهار و تابستان 1389)

Clostridium spp. especially Clostridium perfringens are pathogenic factors in human and animals. It was used novel approach based on polymerase chain reactionnamed densitometry technique for determining of Clostridium spp. bacteria frequency in duodenum, jejunum, ileum and cecum of broiler. Digest contents were removed and extracted their DNA. It was obtained specific bands for detecting of Clostridium spp. and all bacteria using polymerase chain reactions. Then, it was determined relative population of Clostridium spp. relative to total gut bacteria by means of densitometry technique based on 16S ribosomal DNAapproach. Result analysis was showed Clostridium spp. consists 0.36% of total duodenum bacteria. Also it wasshowed Clostridium spp. consists 0.20% of total jejunum bacteria. Meanwhile Clostridium spp. consists 1.15% of total ileum bacteria. Furthermore it was showed Clostridium spp

Furosemide is a loop diuretic that with affecting on ascending part of henles tubule, increase urine volume. With attention to direct effect of furosemide on Na+, K+, Ca2+ and Mg2+ levels and cell functions (neuron, heart, muscle), itseems that the route of administration of furosemide, can influence the level of abovementhioned cations. When this drug can be effective that has minimum changes onelectrolyte levels. In this study we compare the bolus injection versus continues infusion of furosemide on electrocardiogram changes and Na+, K+, Ca2+ and Mg2+levels of serum and urine. In this study, sixtheen domestic male dogs were selected randomally and divided to two groups. In group 1, bolus injection of furosemide (6mg/kg)and in group 2, continous infusion of furosemide with samedose were administrated.

Free radicals can be produced during the respiratory oxidation of different cells.these free radicals can damage to various macromolecules as protein, fat, nucleic acids and…,are harmful for body. The natural defence system that can prevent the damage of free radicals and nuteralize them, have tittled under the name of total antioxidantcapacity (TAC) and probably can change in some stress conditions such as number of parturient. In this respect,we have determined 100 dairy cattle(1 week before and aftercalving) and measured plasma TAC levels in them by ferric reducing ability of plasma (FRAP) method. The results were analyzed by ANOVA_test and siginificantdifference was shown between TAC levels of parturation number 4 and 5 with (p<0.001).also in each parturitionplasma TAC level decreased in after calving to comparebefore same Calving. Vet. Res. Bull. 6,1:19-22,2010.Keywords: TAC,calving,cow

Methotrexate (MTX) is a chemotherapeutic agent that used for the treatment of a variety of tumors and inflammatory diseases. The aim of this study was investigated the effect of growth hormone on histomorphometerical parameters of testis after methotrexate administration in rat. In this experimental study, the eighty male wistarrat were selected and randomly divided into eight groups; control (n=20) and test (n=60). One and two test groups were received 0.3mg/kg GH (IP) for 14 and 28 consequence days, respectively. Three and four test groups were received 1mg/kg MTX (IP) per week for 14 and 28 consequence days, respectively. Five and six test groups were received 1mg/kg MTX (IP) with 0.3mg/kg GH (IP) (MTX+GH) for 14 and 28consequence days, respectively

Mycoplasma synoviae (MS) is one of the main disease in fowl which cause infections of respiratory system and tenosynovitis. Characterization and successful detection ofMS in broiler breeder farms help to prevent distribution of MS in broiler farms.The aim of this study was isolation and characterization of MS in broiler breeder farms by cultureand polymerase chain reaction (PCR) techniques. Thirty specimens which obtained from flocks with positive serum reactions were studied by culture and PCR techniques.After collecting of Choanal cleft and tracheal swabs in PPLO broth, a short-term incubation was occurred and then filtered and a serial passage was performed. In thefourth passage, all of the specimens cultured on PPLO agar, in addition, original specimens used to extract of DNA and MS-PCR technique. Results showed that 15/30were positive by culture (Approved by using of standard MS specific antiserum) and 25/30 were positive by PCR. These observations highlight the higher sensitivity of PCRrather than culture. These results may be observed because of PCR ability to detect DNA after mycoplasma´s death.

this research it is mostly paid attention to the effect of physiologicaly ghrelin and the role of Ghrelin in renal function which is the result of bacterial lipopolysaccharids(LPS) in rats. The number of 70 rats which have divided in 7 group for the experiment. firstly rats were IP injection of (LPS) by the amount of 20mg/kg B.W inducedendotoxemia effects. Moreover Ghrelin by the amount of 4nmol befor and after the injection of LPS which is done by injection IP method.

Bovine Immunodeficiency Virus (BIV) belongs to the genus Lentivirus of the family Retroviridae comprising 3 main structural genes of gag, pol and env. For determination of genetic relationship of env gene of BIV in Iran with those in other countries firstly fragments 490 bp corresponding to env from 5 samples amplified in PCR system and was sequenced for determining nucleotide sequence and was compared with identified nucleotide sequence of this gene in other countries

Newcastle disease is one of the infectious diseases in birds. Doing an accurate vaccination in a proper time is an approach to prevent the disease. This study clarified theeffect of eye drop route vaccination scheduling with Newcastle Avinew vaccine on stimulating of humoral immunity in broilers by Hemagglutination Inhibition testin order to find a better vaccination protocol. So, 150 oneday- old broiler chicks were divided into 3 separated groups. Treatment groups were consisted of A1 (eye dropvaccination at 1st, 14th day old regardless of maternal antibody), A2 (eye drop vaccination at 7th, 17th day old with considering maternal antibody) and controlgroup(without vaccination). Results showed the decreasing trend at 1st,7th,14th days old in all groups and increasing trend after 21th day in groups A1 and A2.Control group had the decreasing trend in all bleeding stages and reached to the lowest level at 35 th days old. Statistical analysis revealed no significant differencebetween A1 and A2 groups (p<0.05). Also, there was no significant difference between control group and the other groups up to 14th day old. However, considering theresults of this study, eye drop vaccination at 1st day of life in high risk regions should be implemented in order to prevent Newcastle disease, regardless of maternalantibody.