مجله سلول و بافت
سال یکم شماره 2 (زمستان 1389)

The aim of this research is evaluation of auxin changes in plant regenerated from tobacco root with Ri-TDNA

In this research leaf segments of tobacco were transformed by Agrobacterium rhizogenes. Transformed hairy root was selected by their ability to grow on medium containing Kanamycin. For confirmation of transformation x-gluc substrate was used. From transformed roots at first callus and then plant was regenerated. Finally auxin content from roots and leaves of regenerated plants were measured.

Blue color of roots confirmed the successful transformation of samples. Results showed that auxin content in transgenic plants compare to control was increased 80-90 %. Transgenic plants showed shorted internodes and smaller leaf area than non transgenic plants.

Regenerated plants from transgenic roots showed higher level of auxin content than non transgenic plants. Change of auxin content and its interaction with gibberellic acid possibly resulted in shorter length of transgenic plants.

In this study, the effects of Ni and Zn to leaf anatomy, some flavonoids and nitrotoxin of Coronilla varia were investigated. 40 days aged grown Coronilla varia L. plants in equal growth condition were treated with different concentration of ZnCl2 (0, 7.5, 15, 22.5 and 30 mM) and NiCl2 (0, 2.5, 5, 7.5 and 10 mM) for 24 and 72 hours in hydroponic culture and the effects of imposing heavy metals on Leaf anatomy, some flavonoids of control and treated plants examined and compared using 2-DPC (Two Dimensional Paper Chromatography) and TLC (Thin Layer Chromatography) methods. Nitrotoxin measured using spectrophotometer too. All of Data compared together. Results showed anatomical changes in polluted plants responses to Zn and Ni. In general results showed a wide changes in leaf flavonoids profile with increases number of total flavonoids at Zn and Ni stress. However, Nitrotoxin increased significantly when Zn and Ni increased. Probably, C. varia L. can tolerate against heavy metals stress of Zn and Ni with changes of flavonoids content and nitrotoxin.

The aim of this research is the investigation of anti- inflammatory effect of silymarin on the treatment of colon ulcer induced acetic acid in mice Balb/C spicies. In this study, 32 mice are divided into 4 groups (n=8).These groups include: control group with no colitis report, the Sham group with colitis report that are not treated and the groups with colitis report that received 40 and 80 mg/kg B.W. of silymarin orally.The groups with silymarin treatment initially received silymarin for 14 days before induction of the disease and then for 1 week after effected by disease. The drugs are fed to the mice per oral. To induce colitis, 1 ml of acetic acid (%4) is injected directly into the rectum. In the one week after induction of colitis, the animals sacrificed and the damages of the colon investigated with Murthy method. Also, the concentration of alkaline phosphatase enzyme measured. Also, tissue oedema verified. The results shown the concentration of alkaline phosphatase enzyme has been increased significantly (P<0.05) in colitis group in comparision with control group whear as the concentration of these enzyme has been decreased significantly (P<0.05) in treatment groups in comparision with colitis group. Also, acetic acid causes severe inflammation and damages of the colon. It is concluded treatment utilized silymarin can be considered as a suggestive way.

The aim of this study was to ciprofloxacin labeling by technetium99m and its bio distribution in normal and infected laboratory animals for the site of infection detection. labeling of ciprofloxacin by 99mTecnhetium at best concentration (2mg) and tin chloride at 50-600 micrograms concentration at different temperatures as well as pH using thin layer chromatography and different solvents were evaluated. Heart, lung, stomach, intestine, kidney, blood, spleen and muscle tissues separated and counted by HPGe. Radiochemical purity found to be not less than 90% and serum stability was the same at least for 1hr bio distribution studies showed the muscle at 1 and 4 hrs. Were observed after injection. Ciprofloxacin labeling by direct method in high radiochemical purity may be used as a formulated compound for the site of infection diagnosis in experimental animals.

Regarding the role of vitamin B12 in regulating the immune reactions, this compouned is used in the present study as an accessory agent with the honey bee venom for reduction of inflammation. This study has been down for checking effects of bee venom and B12 on inflammation and gliosis in experimental allergic encephalomyelitis rats. The guinea pig spinal cord homogenate (GPSCH) along with the Complete Freund’s Adjuvant (CFA), was used for induction EAE in Lewis rats for creating a model of MS.EAE was induced in 40 rats, randomly divided to four groups and treated with honey bee venom and vitamin B12. The treatment started from the first day post immunization by GPSCH-CFA and continued for ten days. Immunohistochemical method used for study glial fibrillary acidic protein (GFAP) and ELISA was used for assessment serum TNF-α.. Our data showed that treatment with the honey bee venom and vitamin B12 decreased the disorders of clinical scores, serum TNF-α and level gliosis in Lewis rats induced by GPSCH-CFA. Combination of honey bee venom and B12 has anti-inflammatory activity by inhibiting generation of autoreactive T cells and decreases CNS and gliosis damages.

The aim of this study is to examine the efficiency of DNA absorption and gene transfusion to chicken hepatoma cells line LMH at both transfection and transduction levels using recombinant lentiviruses. Then transgene expression level was studied.

The cells were cultivated in 96-well plates for viral transfection or transduction and then studied using fluorescent microscope in different stages. Green Fluorescent Protein-positive (GFP) cells counted by Grid Cell Counter software and transgen expression level calculated using ImageJ software. Data analyzed using SPSS.

GFP gene expression began 6 and 9 hours after transfection in HEK and LMH cells, respectively. These times were increased, respectively, to 22 and 36 hours post-transduction. Counting GFP+ cells 48 hours post-transfection showed that HEK and LMH cells have received and expressed 40% and 35% of DNA. These levels increased to 95% and 48% respectively in 72 hours. Also counting HEK+ cells at 72 and 96 hours viral post-transduction confirmed the similar pattern of receiving DNA by both HEK and LMH cells. Therefore, analyzing of GFP expression level showed that HEK and LMH classes expressed GFP in 74% and 89% (P<0.01 significant).

Overall, our results indicated that HEK cells have higher potential to absorption of foreign genes whereas the intensity of expression is higher in LMH cells.

In this study we have used two wheat varieties with different tolerance to salt in different concentration of solt. Giza 168, Seds 1 plants have been grown for seven days in Hoagland solution and some of them were grown in Hoagland solution supplemented with 200 mM extra salt. Cutted leaf segments from these plants incubated with 600 mM salt. Dark-grown pre-adapted, with 200mM extra salt, plants were illuminated to determine the chlorophyll accumulation in two varieties. The maximum photochemical efficiency, Fv/Fm, stayed the same for all measurements. Measurement of relative ETR showed that the tolerant variety, Seds 1, had higher values for ETR than the susceptible variety (Giza 168). Also the measurement showed that the salt adapted samples of both varieties better withstood the excess of salt applied than the non-adapted samples.The chlorophyll content was higher for the tolerant variety compared to the susceptible. The chlorophyll accumulation of the tolerant samples pre-adapted with or without salt showed no dramatic differences. The susceptible sample, Giza 168, however, accumulated more chlorophyll if pre-adapted to salt before being exposed to high salt concentration. PSII seems to be stable and unaffected at the functional level by salt stress. The tolerant variety is better adapted to salt stress. It seems to be due to the advantage of having a more efficient photosynthetic apparatus.

Alpha smooth muscle actin (α-SMA) is an actin isoform present in many kinds of eukaryotic cells. In this survey the expression of this protein in skin appendages was examined. In present study we examined the expression of this protein in 3 populations of dermal cells derived from three different skin appendages in vitro and in one case in situ by means of immunohistochemistry.Observations: The results obtained here showed that in culture this protein is expressed nearly in all dermal papilla cells derived from vibrissa follicles. In case of dermal cells derived from claw unit a high percentage of cells expressed the protein, but in feather follicles only a small percentage of dermal papilla cells were positive for this protein. The α-SMA was also strongly expressed in dermal components reside at basal region of feather follicles in situ. Here we report for the first time that α-SMA is expressed in cultured dermal cells of claw and feather follicle. Moreover, these findings along with the results provided by other workers reinforce the crucial role of this protein in normal activities of skin and cutaneous appendages in mammals and other vertebrates. Given to a high expression of this protein in dermal cells of feather follicle particularly in situ, it can be concluded that α-SMA plays a more important role in birds compared to its role in hair follicle of mammals but the exact role of this protein needs to be elucidated.