مجله سلول و بافت
سال دوم شماره 1 (بهار 1390)

This study examined the effects of vitamin A Chromosomal damage induced by the inhibition of cell phone radiation on the bone marrow erythrocytes on mice. In this experimental study 48 male rats BALB/c were dividedrandomly into four groups: control, laboratory sample1, 2and the empirical distribution, the control mice were kept in natural condition, samples were first for four consecutive days and every day for three hours (12-9) in vitro and cell phone signals within the passive mode (OFF), were studied The mice in laboratory sample 2 were exposed to mobile phones in vitro within three hours (12-9) for four days and the samples of experimental group were injected with15,000 Iu/kg vitamin A intraperitoneally for five consecutive days and on the second day they were exposed to mobile phone inactive mode (ON)for four consecutive days and every day for three hours (12-9). All different groups of mice were described and micronucleus test of polychromatic erythrocytes in bone marrow of mice was performed. Quantitative data obtained with software Spss and were analyzed by ANOVA test (p <0.05). The frequency of micronucleus in polychromatic erythrocytes in bone marrow of experimental mice (25/23) compared with controls (68/34), group 1 (88/35) and control 2 (55/45) showed a significant reduction said. The group 2 compared with control groups has shown significant increase in the number of micronucleus. Vitamin A reduces the in duction of chromosome damageinduced by waves in the mobile mouse bone marrow polychromatic cerythrocytes of adult male BALB/c.

Smoking impairs muscle protein synthesis and increases the expression of myostatin in skeletal muscle. The aim of this study was to evaluate the effects of 8 weeks resistance training on skeletal muscle mass and serum myostatin levels in middle-age smokers and non-smokers. In this quasi – experimental study with pretest – posttest design, fifteen smokers (≥10 cigarettes/day for ≥1 years) and fourteen non-smokers men matched by age (36.06±5.09 and 35.51±5.17 yr, respectively), and body mass index (28.51±1.77 and 28.33±1.83 kg/m2, respectively) were recruited. Resistance training program was performed 50-60 min/d, 3d/wk, for 8 weeks. Serum myostatin levels and body composition (DEXA) were measured before and after the intervention. After leg press resistance training, bench press, and skeletal muscle mass were significantly increased in both groups (p<0.05). Fat indices (e.g., body mass index and fat mass). however; did not change in response to resistance training (P>0.05). Concurrently, serum myostatin levels were significantly decreased in both groups (p<0.05). Short term resistance training caused an improvement in muscle mass and strength in middle-age smokers and non-smokers, and this improvement were accompanied by decreases in myostatin serum levels.

Main goal of this investigation were verification of gene's behavior which located on RNO15 with allelic imbalance during Endometrium cancer development. BDII/Han females were crossed to males from two other inbred rat strains known to have low incidence of EAC (BN/Han and SPRD-Cu3/Han). To generate F2 rats the F1 animals were intercrossed. In addition, backcross populations were generated by crossing male F1 rats to female BDII rats. In order to characterize these tumor-specific genetic aberrations in greater detail, we applied molecular cytogenetic methods (FISH, chromosome painting) to 18 rat endometrial adenocarcinoma (EAC) cell cultures. Analysis in the tumors by FISH analysis showed the presence of small regions of amplifications. These changes were cryptic so that they occurred inside cytogenetically normal-looking chromosomes. We detected rather specific chromosome aberrations (translocations, deletions, amplifications) often leading to copy number changes in small DNA segments (gains, losses).

The aim of this study was to determining effect of short-term garlic extract supplementation on serum total antioxidant capacity(TAC), malondialdehyde and and total peripheral leukocytes counts in non-athlete men after an aerobic exercise. Twenty non-athlete men (aged 23±3 years, body fat 16±2%, and VO2max 40±3 ml/kg/min) in a randomized and double-blind design were divided in two equal groups: supplement group (garlic extract, 700mg/day) and placebo group (dextrose, 700mg/day). After supplementation period, all subjects were participated in aerobic exercise protocol with75% VO2max on the treadmill for 30 minutes. Primary blood samples in the basic state and before of start supplementation, second blood samples after the end of supplementation period and third blood samples after the exercise were taken. Data were analyzed by repeated measure ANOVA, Bonferroni and independent t test using SPSS at α≤0.05. Show that short-term garlic extract supplementation had significant effect (P<0.05) on basal serum TAC. Moreover, MDA, total peripheral leukocytes counts was significantly increased and TAC was significantly decreased (P<0.05) after 30 min aerobic exercise. The change range in the oxidative and inflammatory indices of placebo group, However, was significantly more than in supplement group (P<0.05). Our results suggest that the increase of resting total antioxidative capacity following Garlic short supplementation can cause decrease exercise-induced oxidative damages (Lipid Peroxidation) and inflammatory indices (Leukocytosis) in non-athlete men.

The histological events in experimental studies of interaction between blastema tissue and decellularized bladder of New Zealand rabbit invitro was the aim of this investigation. In order to decellularization of rabbit’s bladder, in physical way, the pieces of bladder tissue were frozen at -4°c for 24 hours, and kept in liquid nitrogen. In chemical Method, the bladder in 1% wt/vol solution of sodium dodecyl sulfate (SDS) for 24 hours to decellularization was kept. For blastema tissue preparation with the help of a special punch, New Zealand rabbit ear (pinna) was pierced, after 72 hours with the help of another puncher, a circular blastema from the edge of holes was separated and the decellularized samples were kept in to the middle of the blastema ring and then, transfered to the culture media in a sterile condition. The samples with hematoxylin & eosin, pick indigo and pickrofushin (van gaison) were stained. In this study, the studies of light microscopy showed that, colonial cells that connected together were perfectly migrated inside the scaffold on the day 15. On the same day, the blastema cells were differentiated to epithelial demensional matrix cells and bladder transitional epithelium created in the center of scaffold. Angiogenesis was observed in scaffold on the next days. It appears, the decellularized bladder of New-Zealand rabbit as a bio-scaffold is capable to provide a proper environment, induce blastema cells to migrate into the scaffold and create connections between blastema cells and differentiation.

Electro magnetic fields (EMFs) on spermatogenesis, blood serum protein level and alkalin phosphatase of mice have been investigated. Male adult Balb/c were arranged in 3 groups (control, sham-exposed, experimental-n=6-). In experimental group, mice received EMFs(120 gauss and frequency of 100 Hz) twice every time 7 hours in two consecutive days. After 48h, the animals were be weighed and dissected. The number of germ cells in testes with histotechnic assay, assessment of alkalin phosphatase with auto analyzer and measurement of blood serum protein with electrophoresis assay were investigated. No significant difference observed in mice weight and testes weight. The number of spermatogonia and secondry spermatocyte showed significant increase (p≤0.05) in experimental group rather control, sham-exposed. Assessment of serum proteins indicated between 5 essential proteins in blood, percentage of alpha-globulins showed significant reduction (p≤0.05) in experimental group. Evaluation of alkalin phosphatase didn’t show significant difference. Exposing Balb/c mice to EMFs with low frequency (120 gauss and frequency of 100 Hz) has led to changes in testis tissue such as increasing secondry spermatocyte, syncitium state in spermatogenic cells, distruction interstitial tissue.These changes can cause reduction of fertility.

Air pollution is a global problem that has negative effects on living organisms. The aim of this study was to investigate the effect of water soluble fraction of diesel exhaust particles (DEP) on some growth factors and scale of bean’s protein. Bean plants were cultivated in experimental condition and sprayed with 30 ml of different concentrations of DEP (0.1, 0.3, 0.6 g/L) and control plants were sprayed with distilled water. Growth factors such as wet and dry weights, length of legume, legume dry weight, seed germination and leaf area in DEP-treated and the control groups were compared with SPSS software and variance analysis. Pollen and seed proteins were studied with electrophoresis method. DEP treatments caused to decrease wet weights of the all DEP-treated plants (P≤0.05), but the other factors (pod length, dry pod weight, pod weight, seed germination and leaf surface) only in the treated group with a 0.6 g/L DEP was significantly reduced. Seed protein profiles were similar in the experimental and control samples, but an increase in the density of a band with molecular mass of 62 kDa was observed in the group treated with 0.6 g/L of DEP. In protein profile of pollen, a band with molecular mass of 55 kDa was disappeared and a new band with molecular mass of 80 kDa was formed. With the consideration of reported detrimental effects for DEP, it seems that the bean plants are resistant that can be related to changes in protein banding profiles and cellular mechanisms of DEP detoxification.

The aim of this essay was to assess the ointment effects of alcoholical extraction prepared from annual leaves of Verbascum speciocum grown in 'Bouein Zahra' area, on skin lesions made in Wistar rats. In this study, at in vivo condition, 36 adult male Wistar rats were divided in 3 groups: Control (no treatment), Sham (treated with petroleum jelly) and Experimental (treated with ointment verbascum extract in different doses). The operation day was considered as day zero. 10 days after treatment, up to the day of 14th, after the surgery on the killed rats, the samples were collected from wound part of each rat for histological analysis and also collagen special staining and average of epidermis thickness, and hair follicle & blood vessels diameter were done. There was an increase in hair follicles’ diameter in experimental groups with 1/3LD50 Ointment comparing with control groups also a significant increase was found in skin epidermis thickness in experimental groups with 1/4 LD50 versus control groups. Moreover a significant increase was observed in blood vessels’ diameter in experimental groups exposed to 1/3LD50 ointment to control. In this study, we realized that the external use of V. speciocum extract on skin lesion with high dosage has a cytotoxic effects and were shown necrosis in the treated tissues but the lower dosages of extraction have been shown better wound healing.