مجله تحقیقات آزمایشگاهی دامپزشکی
سال سوم شماره 1 (بهار و تابستان 1390)

In this study, genital system of 13 suffolk infertile ewes were referred to department of veterinary pathology, faculty of veterinary medicine, the university of Tehran. The tissue specimens were checked for macroscopic lesions and fixed in 10% of buffered formalin. In microscopic examination of endometrium, especially apex of cruncles, showed melanosis in 53.84% of cases. Weren’t seen any lesion in ovaries and fallopian tubes. In microscopic examination in 8 cases (61.5%), chronic endometritis with infiltration by lymphocytes and plasmacells was the most important lesion. In 46.1% of cases, periglandular fibrosis was observed that 50% of them were accompanied by endometrial interstitial fibrosis.The most important finding was chronic endometritis which confirms the results of other researches.

Iran is one of the importanct centers for breeding sheep and goats. Najdi and native goats in Khouzestan province are one of those Iranian goats which have litter size and their average litter sizes are 46 and 26 percent respectively. It is reported that Booroola gene has additive for litter size and ovulation rate in goats. In this study 100 blood samples were collected of Najdi (50 goat) and Native goats (50 goats). DNA of blood samples was extracted by modified salting out method. The DNA was evaluated by spectrophotometer and agarose gel. Site of mutation was amplified using specific primers and PCR product (190 bp bands) was determined by agarose gel electrophoresis, and then the products PCR were digested with AvaII enzyme. The homozygous carrier goats with FecB mutation (BB) have a 160 bp and 30 bp bands, the noncarriers (++) have a 190 bp bands, whereas heterozygotes (B+) have 160, 30 and 190 bp bands. Results ِdid not show any polymorphism for FecB gene in khouzestanian goats. All of goats were wild type (++) so mutation of FecB gene is not cause of prolificacy in khouzestanian goats. Further research is required to evaluate fecundity gene and genotyping of khouzestanian goats.

Planning of Strategic Programs is important in Health management for prevention, control and eradication of diseases. So, strategic programs can be used in planning of strategic plane for diseases especially recurrent and new current diseases. Rinderpest is one of the most important viral diseases. Rinderpest is a contagious disease in cow and buffalo and has eradicated in most of countries in the world such as Iran. Rinderpest is important as a recurrent disease, because of bad health situation in some countries. So, strategic plane is very useful for control and eradication of this disease. This study was performed for planning of strategic program for Rinderpest by epidemiologic and pathologic study for continuance of eradication and prevention of recurrence of the Rinderpest in Iran. In this study information was collected from different studies and analyzed by SWOT matrix. Finally, many prescriptions for continuance of the Rinderpest eradication in Iran as strategic plane, was compiled.

Hydrogen peroxide (H2O2) is a powerful oxidizing agent and could be used in microbial decontamination of chicken carcasses. In this study bactericidal effect of different concentrations of H2O2 in combination with temperature on decontamination of salmonella on broiler skins was studied. 100 µl of bacterial suspension was placed on skin samples, and separately were exposed to 0.2, 0.5, 1, 2 and 3% H2O2 solutions and colony counts were made. Based on the results, the bactericidal effect of 0.2% H2O2 in different temperatures (20, 25, 30, 35 and 40° C) was also studied. Data showed that 0.2% H2O2 significantly (P ≤ 0.05) reduced viable count by 0.85 Log in compared to the water control. Higher concentrations of H2O2 in linear shape were reduced to near 5 logs in viable counts of the bacterium. In sensory assays, samples treated with 2 and 3% H2O2 had a little bite bleached appearance. In subsequent study, H2O2 (0.2%) in 20° C made 0.92 Log reduction in viable counts. In higher temperature (25. 30, 35 and 40° C), this amount was increased to 1.17, 1.58, 1.61 and 1.69 Log reductions respectively. It was concluded that H2O2 could be used in low concentration and in combination with heat treatment for bacterial decontamination of broiler carcasses in abattoirs.

In the present study, a total of two hundred samples (100 samples from carrot juice and 100 samples from cantaloupe juice) were collected and examined by floatation method for Giardia cysts and by modified Ziehl-Nielson staining for Cryptosporidium oocysts. All cantaloupe juice samples were negative for both parasite, but two carrot juice samples (2%) were positive for Giardia cysts (5×10 -2 & 10×10 -2 cysts per cubic centimeter) and thirteen samples (13%) for Cryptosporidium oocysts (7- 1050 oocysts per cubic centimeter). There was no significant association between different localities, hygienic indices of shops (appearance situation, lining walls and floors with tile, existing cashier, using topcoat and hat), contamination rate and infection intensity. Regarding to high contamination rate of carrot juice, its sanitary washing before making juice appears to be essential in reducing infection rate.

Nowadays, Shiga- like toxin – producing Escherichia coli (STEC), verotoxin – producing E. coli (VTEC), are the most important emerged group of food borne pathogens. Cattle, especially young animals, have been implicated as a principal reservoir of STEC. Foods of bovine origin are the major of the infection. The aim of this study was to genotype the O157 VTEC from bovine feces in Kerman city.A total of 142 cattle’s fecal samples were collected from Kerman’s abattoir. All samples were Grew in m TSB and were plated on chrom agar O157, phenol red agar base with sorbitol, and cultured on differential medium for diagnostic of E. coli. The isolates suspicions to O157 were typed with monoclonal antibody again serotype O157. These isolates and feces samples grown in m TSB, were also tested with M-PCR for detection of stx1, 2 genes. One VTEC O157 (0.7%) carrying stx2 and one non- verotoxigenic E. coli O157 (0.7%) were isolated from feces, also five VTEC strains belong to serotypes other than O157 (3.5%) (two isolates carrying stx1, 2 and three samples carrying stx1) were separated from feces which grown in m TSB using d M-PCR. The results of this study showed that the frequency of total of STEC is 3.5% and serotypes other than O157 have higher prevalence. Therefore for the identification of enterohemorrhagic serotypes, bacteriological tests are not satisfactory and molecular tests such as M-PCR are needed for the identification of all serotypes.

Objective of this survey was study on activity and serum levels of hepatic enzymes including: aspartate amino transferase (AST), alanine amino transferase (ALT), alkaline phosphatase (ALP), and Iron in infected cattle with Theileria in Golestan province. A total of 120 blood samples (60 infected and 60 non-infected cattle to Theileria annulata) were collected from different cities of Golestan province (including: Gorgan, Gonbade Kavoos, Agh Ghala, Gomishan, Bandare Torkaman & Azad Shahr) and were studied for protozoa after staining. Also rate of hepatic enzymes and iron were estimated with commercial kits and results were compared with statistical analysis.Results showed that levels of some of hepatic enzymes including: aspartate amino transferase (AST), alkaline phosphatase (ALP) and Iron in infected cattle were more than control group, significantly. But, there was no significant difference in level of alanine amino transferase (ALT) in infected and non-infected animals. Our findings showed that hepatic lesions and anemia have important roles in pathogenesis of theileriosis.

Linguatula serrata is an aberrant arthropod of the class Pentastomida. The adult parasite is found in the nasopharynx of canids. The larval form occurs in the mesenteric lymph nodes of herbivores including sheep in which it develops to the infective nymphal stage. This study was carried out from April 2009 to march 2010 to evaluate the infection rate of Linguatula serrata in 420 sheep which have been slaughtered in Tabriz abattoir. Samples of lymph nodes and livers were examined macroscopically and microscopically. Mesenteric lymph nodes of 162 sheep (38.57%), mediastinal lymph nodes of 43 sheep (10.23%) and livers of 4 sheep (0.71%) were infected with Linguatula serrata nymphs. Results showed that the infection rate increased with ageing (P<0.05). The results also revealed that the infection rate to the nymphs of parasite in black-colored lymph nodes were significantly (P < 0.05) higher than the infection rate in normal-colored nodes. No significant difference was observed between the infection rate in different seasons (P>0.05). Human can be infected through consumption of raw or under-cooked liver and visceral organs of sheep, hence the importance of inspection at slaughter houses needs to be emphasized.