فهرست مطالب
Zahedan Journal of Research in Medical Sciences
Volume:14 Issue: 5, 2012 Jul
- تاریخ انتشار: 1391/04/28
- تعداد عناوین: 8
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Page 1BackgroundMany studies have an emphasis on Reactive Oxygen Species (ROS) formation by electromagnetic field. Camellia sinensis is enriched with antioxidants and the antioxidants can neutralize the effects of ROS. In this study, the effect of Camellia sinensis extract on decreasing the inductive teratogenicity of the electromagnetic field (frequency 50Hz and intensity 50G) in liver and spleen of Balb/C embryonic rat is examined.Materials And MethodsTwenty-four heads of pregnant female rat (Balb/C) were divided into four groups: control group, experimental test group (off-device), empirical group1 (electromagnetic field 50 Gauss), empirical group2 (treated using Camellia sinensis extract + electromagnetic field 50 Gauss), in this empirical-experimental study. Then, liver and spleen tissue cross sections of 19-day embryos were prepared for histological assessments after weight and Crown-Rump length were measured. Resulting quantitative data was analyzed using ANOVA statistical tests and Tukey test with the significance level (p<0.05).ResultsIn examination of tissue sections, mean lymphocyte number of spleen in empirical group 1 showed a significant difference (p=0.001) comparing to the experimental control group, whereas mean lymphocyte number of spleen in empirical group 1 showed a significant difference (p=0.001) comparing to empirical group 2. Mean number of liver hepatocytes empirical samples 2 showed a significant decrease (p=0.004) comparing to the experimental control group, and mean number of liver hepatocytes empirical samples 2 showed a significant decrease (p=0.042) comparing to empirical samples 1.ConclusionConsumption of Camellia sinensis could compensate for the inductive impairments in many cells electromagnetic fields, but it is not recommended in pregnancy period cause of changing the number of some cells.Keywords: Camellia sinensis, Liver, Spleen, Electromagnetic field, Balb, C rat
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Page 7BackgroundThere are different factors which are effective on maintaining homeostasis, especially by pituitary-thyroid axis hormones. The objective of this study was to examine the effect of Physalis alkekengi plant extract belonging to Solanaceae family on the concentration of the pituitary-thyroid axis hormones.Materials And MethodsThis study was conducted on five groups (n=10) of male Wistar rats (with mean weight 210±5g). The control group did not receive any substances, while the reference group received 0.2 ml normal saline daily and the experimental groups received maximum (0.4 g/kg), moderate (0.2 g/kg), and minimum (0.1 g/kg) intraperitoneal (IP) doses of the alcoholic extract for 14 days. At the end of this period, blood samples were drawn and the results were analyzed by SPSS-11.5 software.ResultsThe results of statistical analysis showed significant increases in plasma concentrations of thyroxin (T4) and triiodothyronine (T3) in the maximum dose group (p<0.05) with no significant changes in plasma concentrations of thyroid-stimulating hormone (TSH).ConclusionIncreases in T3 and T4 levels with no changes in TSH concentration indicate hyperthyroidism euthyroidism in which the levels of thyroid hormones increase while the amount of TSH remains constant. These changes could be due to plasma proteins increase including albumin, which are probably induced by physaline and alkaloids existing in Physalis alkekengi. So, these drug doses do not seem to bring about pathological changes in the pituitary-thyroid axis.Keywords: Physalis alkekengi, Rat, Thyroid Stimulating Hormone, Pituitary Gland, Hyperthyroidism
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Page 12BackgroundRegarding the antioxidant property of Otostegia persica extract and the role of antioxidants in Diabetes mellitus treatment, in this study the effect of extract on serum glucose level and renal function indicators was determined in diabetic male rats.Materials And MethodsDiabetes mellitus (type I) was inducted in male rats using intraperitoneal injection of streptozotocin (STZ) (65 mg/kg). To determine blood glucose, urea, and creatinine serum levels; fasting blood samples were collected twice (before STZ injection and 5 days later). The rats with their serum glucose level exceeding 250 mg/dl were considered diabetic and divided into 10 groups separately received Otostegia persica alcoholic extract (100, 200, and 300 mg/kg/day doses), glibenclamide with 600 µg/kg dose and 0.5 ml distilled water for 3 and 6 days using gavage. After 3 and 6 days, blood samples were collected again and glucose, urea, and creatinine serum levels were assessed using spectrophotometry technique by respective kits.ResultsTreating diabetic rats by Otostegia persica extract (100, 200, and 300 mg/kg/day doses) for 6 days results in a significant decrease of glucose and creatinine, yet an increase of serum urea with 200 mg/kg dose. Also, administration of the extract for 3 days (300 mg/kg) reduced glucose, and (in various doses) urea and creatinine serum levels.ConclusionOtostegia persica extract has hypoglycemic effect and administering it in diabetes mellitus not only had no undesirable renal side effects, but also improved renal function to some extent.Keywords: Diabetes Mellitus, Otostegia persica, Kidney
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Page 16BackgroundWound healing in diabetes is one of the challenges in the medical science. Accordingly, new quick wound-healing compounds are welcomed. It is likely that Prosopis farcta - indigenous to southeastern Iran – accelerates regenerating process for its anti-inflammatory, antimicrobial and antidiabetic effects. This study is aimed at examining the local effect of the fruit dusk powder and root extract of Prosopis farcta on diabetic healing.Materials And MethodsTwenty-four male Wistar rats were randomly divided into 4 groups of intact, diabetic control, Experimental group 1(diabetic treated with fruit powder) and Experimental group 2 (diabetic treated with root extract). Diabetic groups were injected streptozotocin (STZ). Then, three holes (4 mm diameter) were made on both dorsal sides of the rats in all groups. Experimental groups were treated by fruit dusk powder and the plant root aquatic extract and the control groups were treated by normal saline. The area of the holes was continuously measured till they were completely closed.ResultsIn the treatment groups using fruit dusk powder and plant root aquatic extract, the hole area had significantly decreased (p< 0.05).ConclusionIt Seems that local administration of Prosopis farcta fruit dusk powder and root aquatic extract probably accelerates healing in diabetic rats. Regarding the antidiabetic, anti-inflammatory and healing effects of this plant, we are hopeful that Prosopis farcta plant finds an appropriate status in traditional medicine and pharmacology.Keywords: Prosopis Farcta, Diabetes, Lesion Healing, Rat
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Page 21BackgroundNon-steroidal anti-inflammatory medicines are widely used today and of their side effects is gastric ulcer. This study is aimed at examining the effect of Carum copticum aquatic extract on healing gastric ulcers inducted using ibuprofen in an animal model.Materials And MethodsThirty heads of adult Wistar female rats were used in this empirical study. Gastric ulcer was inducted using ibuprofen. The animals were randomly divided into 5 groups. Then omeprazole or Carum copticum plant extract was administered (125, 250 and 500mg/kg doses) twice a day for two weeks. In the end, the number and area of the animal's gastric ulcers were assessed. To examine the side effects of the medicine on liver, the amount of liver enzymes Aspartate transferase (AST) and Alanine transferase (ALT) was measured in the animal's serum.ResultsCarum copticum plant aqueous extract had a significant effect on healing gastric ulcers comparing to the control group (p<0.05). The mean number and area of the gastric ulcers in the groups receiving the extract were significantly less than negative-control group (p<0.01). The amount of liver enzymes had also significantly (p<0.05) increased in the groups receiving the extract (250 and 500 mg/kg doses).ConclusionCarum copticum plant seed extract is effective in healing gastric ulcers and its effect is comparable with omeprazole. So, recognition of the extent and mechanism of the effect requires further studiesKeywords: Carum copticum, Gastric Ulcer, Rat, Ibuprofen
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Page 25BackgroundFruits and vegetables are considered as the source of antioxidant and the factor of destroying reactive oxygen species. The effect of this antioxidant might decrease in time. This study was aimed at examining and comparing the antioxidant effect and the level of phenolic and flavonoid compounds as well as allicin level, between fresh and three-month stale Allium sativum (garlic).Materials And MethodsIn an experimental study, the ethanolic extract of bushes of fresh and three-month-old Allium sativum was prepared and their antioxidant activities were assessed in linoleic acid and β-carotene linoleate system. The amount of phenolic compounds was measured by Folin-Ciocalteumethod, based on gallic acid; the amount of flavonol and flavonoid compounds by aluminum chloride base on rutin base; and the amount of allicinby spectrophotometry method. SPSS-15 Software and t-statistic tests were used to analyze the mean difference between the results of two groups and p<0.05 was considered as significant.ResultsThe efficiency of fresh garlic (35.36) in inhibiting the oxidation was higher (p<0.05) comparing to three-month dated Allium sativum (10.2). Phenolic compounds of the fresh garlic (12.61mg/g) were more than the three-month dated Allium sativum (2.89mg/g). The amount of allicin was respectively 15 µg/ml and 8 µg/ml in the fresh and three-month dated Allium sativum (p<0.05).ConclusionThe fresh garlic contains more useful substances and it is recommended to be used in its fresh estate.Keywords: Fresh, Stale Allium, Sativum (Garlic) Extracts, Antioxidant Activity, Phenol, Flavonoid
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Page 30BackgroundDysmenorrhea is among the factors disrupting women's social activities. Selecting medicines with lower side effects are preferred. The objective of this study is to compare between the effect of herbal extracts of Vitagnus and Mefenamic Acid on the primary dysmenorrhea and menstrual bleeding in female students of the University of Medical Sciences, Hamadan in 2009-2010.Materials And MethodsThis Study was carried out on 80 students with primary dysmenorrhea as two-way blind clinical experience. The participants were randomly divided into three groups of receiving Vitagnus drop, Mefenamic Acid capsule, and placebo drop. Severity of dysmenorrhea was assessed using pain severity measurement tool during a cycle before and two cycle later taking the medicine. Data was analyzed using SPSS-16 Software.ResultsIndividual characteristics of the samples were similar. Mean pain intensity and menstrual bleeding in a cycle before the treatment beginning had no significant differences in the three groups, but in two cycles after the treatment beginning, there was a significant difference between the three and the effect of Vitagnus drop was more effective than Mefenamic Acid capsule in mitigating dysmenorrhea (p=0.0001). The two medicines (mefenamic acid and Vitagnus) were effective in reducing menstrual bleeding (p<0.05).ConclusionVitagnus drop and mefenamic acid resulted in mitigating dysmenorrhea and the effect of Vitagnus was more than mefenamic acid. The two medicines (mefenamic acid and Vitagnus) were effective in reducing menstrual bleeding. Accordingly, Vitagnus herbal medicine can be used as the effective and safe medicine for dysmenorrheaKeywords: Primary Dysmenorrhea, Menstrual Bleeding, Vitagnus, Mefenamic Acid
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Page 34BackgroundThe effects of medicinal plants are variable in different conditions. Here, the antimicrobial effect of 15 medicinal plant species and their dependency on the climatic condition of growth in different geographical and ecological areas of Fars Province were studied.Materials And MethodsIn This empirical study, the antimicrobial effect of hydro-alcoholic extract of 15 medicinal plant species was examined against standard bacterial strains comparing to conventional therapeutic antibiotics using disk diffusion assay and serial broth dilution.ResultsAll Extracts were effective against S.aureus ATCC 25923 growth; also Peganum harmala, Myrtus communis, Mentha pulegium, Mentha spp, and Zataria multiflora extracts were observed to have antimicrobial activity against E.coli ATCC 25922. This antimicrobial activity had partially similar results, comparing to conventional antibioticsConclusionMedicinal plants produce various amounts of antimicrobial substances under the climatic and ecological conditions of each zone, which must be considered in manufacturing herbal medicines.Keywords: Medicinal Plants, Anti, Infective Agent, Climatic Conditions Rural