فهرست مطالب

Veterinary Science and Technology - Volume:4 Issue:1, 2012
  • Volume:4 Issue:1, 2012
  • تاریخ انتشار: 1391/10/11
  • تعداد عناوین: 7
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  • Amin Tavassoli, Nasser Mahdavi, Shahri, Maryam M. Matin, Masoud Fereidoni, Fahimeh Shahabipour Page 1
    Extracelllar matrix (ECM) materials from mammalian tissues have been broadly used for tissue engineering for animal models. While decellularization of tissues such as bladder, heart valve, knee meniscus and tendon has been performed, there are a few reports for articular cartilage. In This study we aimed to develop a technique to decellularize bovine articular cartilage as a biological construct for cartilage substitution. For this purpose, different decellularization protocols including freeze/thaw cycles followed by treatment with various concentrations of sodium dodecyl sulfate (SDS) were used. Decellularization was analyzed by histological examination including hematoxylin and eosin and picrosirius staining. Treatment with 2%SDS for 5 to 8h followed by 4% SDS for3h hada significant effect on decellularization process with the minimal effect on the collagen contents. On the other hand, Treatment with 1% SDS had no effect on cell removal and use of 8% SDS for 5 to 8 h resulted in complete elimination of cells and significant decrease in cartilage matrix and collagen contents. This study provides a technique to produce acellular ECM derived from articular bovine cartilage whichmay serve as a xenogenicscaffold for cartilage tissue engineering.
    Keywords: Bovine articular cartilage, decellularized matrix, sodium dodecyl sulfate, tissue engineering
  • Tayebe Zeinali, Saeid Khanzadi, Abdollah Jamshidi, Mohammad Azizzadeh Page 9
    Salmonella is still one of the most important pathogens related with food borne outbreaks.This study was designed to examine the combined effects of different levels of pH (7.4, 6.4, and 5.4), acids (acetic, citric and hydrochloric acid), temperatures (35 ˚C and 25 ˚C), and NaCl concentrations (0.5, 3 and 6%, w/v), on growth of Salmonella Typhimurium in brain heart infusion broth. The experiment conducted is triplicate. Growth was monitored byvisible turbidity over a 30-day period.To evaluate effects of explanatory variable on time to detect (TTD) the bacterial growth, parametric survival models based on the weibull distribution was used. According to our results, the growth of S.Typhimurium was affected significantly(P)
    Keywords: Salmonella Typhimurium, Modeling, Predictive microbiology, Time to detection
  • Rouhollah Dehghani, Mohammad Mehdi Sedaghat, Nader Esmaeli, Ahmad Ghasemi Page 19
    Myiasis is a common veterinary entomology problem in many slaughterhouses in the tropics.The prevalence of myiasis among slaughtered cattlewas investigated in Kashan Abattoir, central Iran, in 2009. All kinds of dipterous larvae that may cause myiasis were collected and identified.Thenthe prevalence of each infestation was analyzed by statistical tests. The results of the study revealed a total of 4 species of dipterous larvae belonged to two families of Sarcophagidae and Oestridae. The identified species were the following ones: Family Oestridae: Hypodermalineatum (61.4%), Hypodermaovis (12.6%), Oestrusovis (22.7%) and Family Sarcophagidae: Wohlfahrtiamagnifica (3.8%).Of the 7736 examined animals, 2.5% of goats and 0.5% of sheep were infested by any kinds of dipterous larvae. Statistical comparisons were made between the sex of host species and occurrence of myiasis, infested animals and species of larvae, the infested body parts of the host, and finally between the prevalence and origin of the cattle. Statistically the possibility of infestation of goats was 1.43 times more than sheep (odds ratio=1.43). These findingscan be considered by officials to keep the human and animal myiasis under control. There is also need to investigate the prevalence and incidence of animal myiasis in other parts of the country to clarify the spread of infestation in the country and in the region.
    Keywords: Myiasis, Oestrusovis, Hypoderma lineatum, Hypoderma bovis, Wohlfahrtia magnifica, Iran
  • Mohammad, Reza Bassami, Abdollah Jamshidi, Aida Kasaei Kasaei, Azam Mohamadi Mohamadi Page 29
    Avian Intestinal Spirochaetosis (AIS) is an intestinal infection caused by anaerobic spirochaetes of the genus Brachyspira, including B.pilosocoli. The purpose of this study was isolation and identification of B.pilosocoli from laying hen flocks, located in Mashhad suburb, KhorasanRazavi province, Iran, and investigating the frequency of the infection. One hundred and eighty cloacal swab samples from 18 randomly selected flocks (10 samples/flock) were cultured anaerobically on selective agar and confirmed as intestinal spirochaete by itsspirichaetal form using phase contrast microscopy. Then, the samples were subjected to PCR amplification followed by DNA sequencing. A total of 24 samples from 8 flocks were selected as suspected cases by culture and phase contrast microscopy. Upon PCR amplification by specific primers, only 9 cultures belonged to 3 flocks appeared to be B.pilosicoli. Sequence analysis of the amplicons confirmed the identity of all isolated ones. Based on the results obtained, it was concluded that B.pilosicoli might be strongly involved in AIS among laying hen flocks of this geographical region. The results could also be considered as an indicator for large scale investigation into the true prevalence of the infection. This study is the first report of infection in laying hens flocks of Iran.
    Keywords: Brachyspirapilosicoli, laying hens, culture, PCR
  • Mohammad Heidarpour, Mehdi Pourtaghi, Javad Khoshnegah Page 37
    The present study was carried out to determine the seroprevalence and risk factors of canine leishmaniasis in a population of owned and strayed dogs in Mashhad, north-east Iran. Of 300 serum samples obtained from dogs, 8.67% (26/300) were positive for Leishmaniainfantum by Indirect Florcense Antibody Test (IFAT). The sero-prevalence rates in owned and stray dogs were 9.5% (19/200) and 7% (7/100) respectively. Significant differenceswere observed between the age groups (P < 0.05); dogs less than one year old showed the highest seroprevalence rate (17.4 %). The seroprevalence rate for leishmania infection in male and female dogs were 10.43 % (17/163) and 6.57% (9/137) respectively. In addition, the seroprevalence rate for leishmania infection in small and large breeds of dogs were 10.5% (6/57) and 8.2% (29/243) respectively. No significant difference was found between sex and breed of the studied dogs. The number of lymphocytes were significantly higher in the leishmania-infected dogs than dogs which were not infected (P)
    Keywords: Canine leishmaniasis, Hematology, Mashhad, Iran, Seroprivalence
  • Mohammad Reza Aslani, Mohammad Heidarpour, Vahid Najar, Nezhad, Masoud Mostafavi, Yaser Toosizadeh, Khorasani Page 47
    Lead poisoning associated to residuals of batteries recycling was diagnosed in a small herd of Holstein cattle. In this outbreak, 10 animals showed clinical signs of lead poisoning, 5 dead and others saved after chelating therapy with CaNa2EDTA.Mean blood lead level of clinically intoxicated cattle was 0.624 ± 0.388ppm and the levels varied between 0.320 and 1.300 ppm. Blood and milk lead levels of 9 exposed but unaffected cattle ranged 0.250 to 0.590 and 0.060 to 0.290 ppm respectively. Although blood and milk lead levels of those cattle sharply decreased after removal of lead source, some of them showed higher than acceptable levels when samples analyzed 60 days later.Products of all lead exposed cattle may not be safe for human consumption for several weeks and analysis should be performed to ensure their safety.
    Keywords: Lead poisoning. Cattle.Battery recycling. Iran
  • Samaneh Ghasemi, Maneli Ansari Mood, Ali Mirshahi, Hossein Kazemi Mehrjerdi, Amir Afkhami, Goli Page 53
    A 2- year- old male, intact domestic short hair cat was referred to the Veterinary Clinic of Ferdowsi University of Mashhad with a history of inappetence, vomiting, dysuria,stranguria, depression and continuous urine leakage from the skin injury in perineal region. After physical examinations, temperature, pulse and capillary refill time (CRT)???were decreased and pale mucosal membranes and dehydration were observed. Palpation of the abdominalregion was painful and kidneys were enlarged.Penis was not present in its normal anatomical position.In biochemical analysis, significant rise in BUN (urea nitrogen) and creatinin were noticed.In abdominal radiographs and ultrasonography evaluations distention of the urinary bladder and blockage in urinary tract were identified. According tothese resultspresence of an obstruction was confirmed in the penile urethra, and consequently, perinealurethrostomy (PU) was performed for correction of obstruction and renewed urine outflow. Normal urination established in our patient after PU surgery and common postsurgery complications did not occurred.
    Keywords: cat, penile urethra obstruction, perinealurethrostomy