فهرست مطالب

  • Volume:13 Issue:4, 2014
  • تاریخ انتشار: 1393/01/31
  • تعداد عناوین: 12
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  • Zohre Jadali, Mohammad Bagher Eslami Pages 220-230
    A central role for T cells and their cytokines in the pathogenesis of psoriasis has been proposed; however, there are controversies over the details of this issue. The goal of this study is to summarise currently available data on the importance of T cells in psoriasis pathogenesis.A systematic review of the English medical literature was conducted by searching PubMed, Embase, ISI Web of Knowledge, and Iranian databases including Iranmedex, and SID for studies on associations between the involvement of T cell subsets and psoriasis.The results of the present study indicate that alterations in the number and function of different subsets of T-cells are associated with psoriasis.It appears that studies on T cell subsets contributed to understanding the immunopathogenesis of psoriasis. In addition, it may have provided novel therapeutic opportunities in ameliorating immunopathologies.
  • Cuicui Duan, Lijie Yang, Aili Li, Rui Zhao, Guicheng Huo Pages 231-239
    Cow’s milk whey consists of many protein components and some of them are antigens to human and known to modulate immune responses. Enzymatic hydrolysis is a useful method to modify proteins with allergenicity. The objective of this study was to identify whether the in vitro enzymatic hydrolysis could reduce the allergenicity of whey protein concentrates (WPC).In this study, WPC were hydrolyzed by trypsin and twenty-four BALB/c mice were divided into three groups and fed with WPC formula and WPC hydrolysates formula, while the control mice received milk-free diet.The results revealed that there was no significant difference between the body weights among all groups. WPC-fed mice produced an elevated spleen lymphocyte proliferation level than WPC hydrolysates-fed mice and also produced higher levels of WPC-specific IgE in intestinal tract and serum in comparison to WPC hydrolysates-fed mice and control group. Significant up-regulation of plasma histamine levels were also observed and showed the same trend with IgE. The secretions of IL-4 and IL-5 were significantly enhanced by WPC. WPC significantly suppressed the secretion of IFN-γ while hydrolysates of WPC significantly increased the secretion of IFN-γ compared to control group.These results suggest that hydrolysis may play a role to reduce the allergenicity of WPC.
    Keywords: Hydrolysis, Hypersensitivity, Mouse, Whey protein
  • Zailatul Hani Mohammad Yadzir, Rosmilah Misnan, Noormalin Abdullah, Faizal Bakhtiar, Brenda Leecyous, Shahnaz Murad Pages 240-246
    Component-resolved diagnosis (CRD) using microarray technology has recently been introduced with the aim to improve diagnosis of allergy. The aim of this study was to compare performance of this allergen microarray to those of an established extract-based skin prick testing (SPT).45 patients with allergic rhinitis were studied (16 children and 29 adults). SPT to Dermatophagoides pteronyssinus, Dermatophagoides farinae and Blomia tropicalis extracts and allergen microarray ImmunoCAP ISAC were carried out for all patients. Forty out of 45 patients demonstrated positive SPT to all mite extracts tested. These 40 patients were considered to be mite- allergic based on the positive SPT results. The remaining 5 patients with negative SPT to any mite extracts were classified as non-mite allergic. Comparatively, based on the microarray results, only 34 mite-allergic patients had detectable serum IgE to at least one of the mite allergen components tested whereas 6 patients with positive SPT to mite extracts showed no detectable IgE reactivity to any of the components tested. One non-mite allergic patient had a positive test- Blo t 5. Der p 10-positive patients also reacted to other cross-reactive tropomyosin from anisakis (Ani s 3) (25%), cockroach (Bla g 7) (50%) and shrimp (Pen m 1) (75%).CRD is a reliable tool for the diagnosis of allergy to mites. Der p 10 might be a useful indicator to identify a subset of mite-allergic patient that have additional sensitization due to cross-reactivity and thus allows selection of patients for immunotherapy.
    Keywords: Allergic rhinitis, Component resolved diagnostics (CRD), Cross, reactive tropomyosin, Immunotherapy, Mite allergy, Skin prick testing
  • Mahshid Sirjani, Foroogh Azam Taleban, Azita Hekmatdoost, Zohreh Amiri, Michael Pellizzon, Mehdi Hedayati, Katayoon Bidad, Raheleh Shokouhi Shoormasti, Zahra Pourpak Pages 247-255
    There has been considerable inconsistency regarding the potential relationship between dyslipidemia and bone metabolism. The inflammatory stimulation through the receptor activator of the nuclear factor kappa-B ligand (RANKL)/ receptor activator of the nuclear factor kappa-B (RANK)/ osteoprotegerin (OPG) pathway could be the infrastructural mechanism for hypercholesterolemia-induced bone loss.In this study, we investigated the effect of dyslipidemia on RANKL and OPG alongside with pro-inflammatory cytokines. Thirty male C57Bl/6 mice (4 weeks old) were randomized to two purified diet groups (15 animals in each group), high fat, low carbohydrate diet (HFLCD) and its matched low fat, high carbohydrate diet (LFHCD). After 12 weeks of feeding in standard situations, the plasma concentration of lipid profile, interleukin (IL)1Beta,, IL-6, tumor necrosis factor-alpha (TNF-α) and RANKL, OPG, and RANKL: OPG ratio were measured.In the present study, although the body weight significantly increased during 12 weeks in HFLCD and LFHCD groups, there were no significant differences in food intake, food efficiency ratio and weight gain between the two groups. The LFHCD group had significantly higher median RANKL and RANKL/OPG ratio. There was no significant difference in plasma IL-1β, IL-6 and TNF-α concentration between LFHCD and HFLCD groups.These unexpected findings from LFHCD, that seem to be as a result of its higher carbohydrate proportion in comparison to HFLCD, implicate dietary carbohydrate rather than dietary fat as a more significant nutritional factor contributing to change in RANKL level and RANKL: OPG ratio.
    Keywords: Bone, Cytokines, Diet, Dyslipidemia, Fat, RANKL
  • Soodeh Razeghi Jahromi, Seyed Rafi Arrefhosseini, Amir Ghaemi, Akram Alizadeh, Hedieh Moradi Tabriz, Mansoureh Togha Pages 256-264
    Experimental allergic encephalomyelitis (EAE) is considered as the murine model of multiple sclerosis. Daidzein a phytostrogenic compound of soy is known to impose immunomodulatory and antioxidative effects. We conducted this study to assess the potential protective and therapeutic effects of daidzein on allergic encephalomyelitis.C57BL/6 mice were induced with allergic encephalomyelitis using myelin oligodendrocyte glycoprotein (35-55) and received daidzein or dimethyl sulfoxide as the vehicle control. To assess the protective effect of daidzein, the mice were administered with 20 mg/kg of daidzein from 21 days prior to 21 days post EAE induction on a daily basis. To evaluate the therapeutic effect of daidzein, mice were fed with 300 mg/kg daidzein after the appearance of the first clinical signs for 10 days. One day after the last gavage, the mice were sacrificed. Spleen and brain were removed for further histological and immunological analysis.Feeding mice with low dose of daidzein prior to disease induction did not affect disease severity. However, treating with high dose of daidzein after the onset of the disease reduced interferon-γ and interleukin-12 secretion, enhanced interleukin-10 production, suppressed lymphocyte proliferation, and decreased cytotoxicity as judged by lactate dehydrogenase release.In conclusion, daidzein reduced the extent of demyelination and disease severity. Chronic oral therapy with low dose of daidzein did not prevent experimental autoimmune encephalomyelitis. However, high doses of daidzein could prohibit disease exacerbation.
    Keywords: Daidzein, Experimental allergic encephalomyelitis (EAE), Multiple sclerosis, Soy, Immunomodulation, Interferon, gamma, Isoflavones
  • Behzad Heidari, Reza Amin, Sara Kashef, Soheila Alyasin, Mozhgan Moghtaderi, Maneli Aminshahidi, Mehdi Kalani Pages 265-270
    Inflammation of blood vessels is a characteristic feature of Kawasaki disease. Neutrophils play a key role in the inflammatory responses where movement of neutrophils toward the site of inflammation depends on CD11b/CD18 expression as adhesion molecules on these cells. The purpose of this study was to investigate CD11b/CD18 expression in patients with Kawasaki disease upon diagnosis and after treatment.The study included 20 children with Kawasaki disease aged from 3 months to 8 years. Mean fluorescence intensity of CD11b levels on diagnosis and at 1-2 and 6 weeks after intravenous immunoglobulin (IVIG) therapy was measured in these patients. Level of CD11b was measured in age-matched healthy children and febrile children (each 21) as negative and positive controls, respectively.Mean fluorescence intensity of CD11b in Kawasaki patients was lower than that of the control groups before and after 1-2 weeks of IVIG therapy. There were no significant differences in CD11b in Kawasaki patients either with aneurysm or without aneurysm.The CD11b levels at the diagnosis time and after treatment with IVIG in our patients with Kawasaki were lower than the control groups.
    Keywords: Adhesion molecules, CD11b, Flowcytometry, Integrin, IVIG, Kawasaki disease, Vascular aneurysm
  • Mohammadali Saba, Mohammad Reza Sharif, Hossein Akbari, Hassan Nikoueinejad, Maryam Ramazani Jolfaii Pages 271-277
    It has been suggested that elevated serum level of YKL-40 could be a marker for asthma and its severity. Along with few published studies, we investigated its correlation with asthma and its severity as well as spirometric indices.114 patients with asthma and 114 healthy controls underwent the assessment of serum level of YKL-40 (by ELISA) and spirometric indices. Pearson''s coefficient determined the correlation between the variables and multivariate linear regression analysis was used for adjusting the effect of different probable confounding factors.Serum levels of YKL-40 were significantly higher in the asthmatic patients compared to those in healthy people (p<0.001). We also found a significant correlation between YKL-40 serum level and spirometric indices even after adjusting the effects of other variables.We report for the first time in an Iranian population that YKL-40 may be a good diagnostic marker of asthma in serum.
    Keywords: Asthma, Respiratory Indices, YKL, 40
  • Marzieh Tavakol, Payam Mohammadinejad, Ilaria Baiardini, Fulvio Braido, Mohammad Gharagozlou, Asghar Aghamohammadi, Mohammad Nabavi, Abbas Dabbaghzade, Zahra Tavakol, Mohsen Afarideh, Nima Rezaei Pages 278-285
    Chronic urticaria (CU) also known as chronic idiopathic urticaria results in a lowered quality of life (QoL). Disease specific questionnaires are necessary to assess QoL in CU patients. Chronic Urticaria Quality of Life Questionnaire (CU-Q2oL) is the only available and validated disease specific questionnaire in the assessment of QoL in CU patients originally developed in Italian language. The aim of the current study was to develop the Persian version of the CU-Q2oL with an acceptable reliability and validity.Using the standard methods provided by guidelines, CU-Q2oL was translated into Persian. A total number of 110 patients with confirmed diagnosis of CU were asked to fill the questionnaire. Determination of scales was performed in addition to checking the data for internal consistency and known group validity. Urticaria activity score 7 (UAS7) was used to assess the severity of the CU in the population study. The 6 dimensional scale of Persian CU-Q2oL was determined using the Exploratory Factor Analysis. About 68% of the variance was explained by these 6 factor structure higher than 59.9% of the original Italian version.All 6 factors showed acceptable internal consistency as measured by Cronbach α coefficient. There was a significant correlation between UAS7 and total CU-Q2oL score. UAS7 and the presence of angioedema were predictors of CU-Q2oL score.The Persian version of CU-Q2oL was shown to be a valid and reliable tool to be used in the future clinical studies. Cultural considerations must be kept in mind in adoption of CU-Q2oL to other languages.
    Keywords: Adaptation, Chronic urticaria, Persian, Quality of life, Questionnaire, Validation
  • Fariborz Zandieh, Bahram Mirsaed Ghazi, Anahita Izadi, Mohammad Gharegozlu, Motahareh Aghajani, Mahdi Sheikh Pages 286-289
    Papillon Lefevre Syndrome (PLS) is a very rare genetic syndrome that only less than 500 cases have reported in the world. Patients have a typical cutaneous involvement with hyperkeratosis especially on the soles and palms and early shedding of primary teeth. Internal organs involvement such as liver abscess has been presented as case reports. This is for the first time that a genetically documented PLS with footsteps of mycobacterium tuberculosis in liver and kidney will report.
    Keywords: Mycobacterium tuberculosis, Papillon, Lefevre syndrome
  • Nicolae Ovidiu Berghi Pages 290-295
    Opening frontiers has led in the last two decades to a massive migration of Romanians to Spain and Italy, two countries known to have large areas planted with olives. Romania owns large areas planted with ash forests. Because of cross-reactivity between these two trees belonging to the Oleaceae family and the big allergenic potential of olive pollens, after a number of years of residence in these countries, many Romanians will present allergic respiratory symptoms of rhinitis and asthma, both in the season of olive pollination and ash pollination.
    Keywords: Cross Reaction, Italy, Oleaceae, Pollen, Romania, Spain
  • Muammer Kara, Alpaslan Tanoglu, Ali Kutlu, Ozgur Sirkeci, Murat Kekilli Pages 296-297
    Proton pump inhibitors (PPIs) are widely prescribed drugs in daily practice. Allergic reactions, even small number of anaphylactic reactions to PPIs have been reported. Omeprazole, lansoprazole, pantoprazole, rapeprazol and esomeprazole are classified in the same group. Despite the similarity of biochemical structures among these drugs, presence of cross-reactivity between PPIs is controversial.1,2 In this letter, we present 3 lansoprazole allergy cases, who were prescribed and took esomeprazole safely after allergic reactions to lansoprazole.
    Keywords: Allergic reactions, Esomeprazole, Lansoprazole allergy, Proton pump inhibitors