Novelty in Biomedicine
Volume:2 Issue: 3, Summer 2014

Trastuzumab is a monoclonal antibody that is used in treatment of breast cancer. We labeled this monoclonal antibody with Technetium-99m and performed in vitro and in vivo quality control tests as a first step in the production of a new radiopharmaceutical. Trastuzumab was labeled with Technetium-99m using Succinimidyl Hydrazinonicotinamide (HYNIC) as a chelator. Radiochemical Purity and stability in buffer and serum were determined. Immunoreactivity and toxicity of the complex were tested on SKBR3, MCF7 and A431 breast cancer cell lines. Biodistribution study was performed in normal mice at 4 and 24 h post injection. The radiochemical purity of the complex was 95±1.4%. The stabilities in phosphate buffer and in human blood serum at 24 h post preparation were 85±3.5% and 74±1.2%, respectively. The immunoreactivity of the complex was 86±1.4%. The binding of labeled antibody to the surface of SKBR3, MCF7 and A431 cells were increased by increasing the human epithelial growth factor receptor 2 (Her2) concentration on the cells surface. These findings showed that the new radiopharmaceutical can be a promising candidate as Her2 antigen scanning for human breast cancer.

Social health as third dimension of health, along with physical and mental health, has drawn more attention in recent years among policy makers and health system managers. No other study, according to our review, has documented measuring individual-level social health in Iran. In response to this need, our study tends to assess Iranians self-rated social health through conducting a survey in 3 cities of Iran. We conducted a survey using cross sectional method in three cities of Iran (Tehran, Isfahan, Urmia) included people more than 18 years old. We use a random sample size of 800 people. The scale provides a total score of social health and three sub-scores. Total score was calculated by summing all 33 items, so the range was between 33 to 165, considering that higher score indicating better social health. Psychometric parameters of scale were acceptable. To interpret scores, respondents were categorized into five ordered groups as quintiles for amount of social health. To compare social health scores in different demographic groups multiple linear regression was employed to interpret association between demographic variables and social health score. From a pool of 800 persons, 794 (99%) agreed to participate and filled out the questionnaire completely. The mean of self-rated social health score was 105.0 (95% confidence interval, 103.8 to 106.2). 50% of participants had medium level of social health. Social health score was higher for those who live in Uremia as a small city in comparison with big cities-Tehran and Isfahan (p<0.001) and was lower for unemployed people (p=0.029). There was no association between social health score and other factors such as sex, age and educational level (p>0.05). This study may be considered as the first step in evidence-based policy-making in the field of social health in Iran. Certainly, it is necessary to conduct more studies to measure social health and its determinants in a nation-wide approach.

In this study, antimicrobial effect of total extract of Tribulus terrestris L. and its fraction containing Benzoxazine derivative (Terresoxazine) was studied for the first time in Iran. Total aqueous extract of plant was prepared and in order to separate the components, liquid/liquid extraction method with Petroleum ether was used. Liquid chromatography-mass spectrometry (LC/MS) system proved the existence of Benzoxazine derivative in the water and the third fractions. Antimicrobial effects of all extracts were examined against 10 Gram positive and negative and candida spp. by cup plate and disk diffusion methods. Also, the minimum inhibitory concentration (MIC) was determined by micro dilution method. The total extract showed antibacterial effect on Escherichia coli, Pseudomonas aeruginosa and Bacillus subtilis. The fraction of Benzoxazine had no effect against tested microbes. MIC and minimum bactericidal concentration (MBC) determination showed that B. subtilis had the low sensitivity to the total extract. Beside, total extract in water with 1000 mg/ml concentration and total extract in Dimethyl sulfoxide (DMSO) 10% with 750 mg/ml density can be substituted to Penicillin 200 mg/ml to combat the P. aeruginosa infections. Because of antibacterial effects of Tribulus terrestris L. against both Gram negative and positive bacteria, and no antibacterial effect of the fraction containing Benzoxazine derivative, it can be concluded that antibacterial effects of the total extract is due to other active ingredients or it is because of the cumulating of different components in total extract. Therefore separation of other components of total extract and determination of their antibacterial effects can be future subjects for researches about this plant.

Hepatitis B is considered as one of the most common infectious diseases. The contamination of this virus has caused hygienic problems all over the world. Liver cirrhosis and hepatocellular carcinoma are most important consequences of this disease. The purpose of this study is to determine the rate of infection in Tabriz North West of Iran. This study is carried out, on partial and short time basis between 2010 and 2011, on test results of 21421 examined individuals whom have visited clinics in Tabriz. HBsAg of the samples was analyzed with chemiluminescence apparatus and finally, SPSS software was used to conduct the statistical examination of the results. Among 21421 examined individuals (5021 cases in 2010 and 16400 cases in 2011), 594 samples (2.77%) were reported positive and 20827 samples (97.23%) were reported negative. According to the results, gender was not a determining factor affecting the susceptibility of patients. Our country is endemic to this disease, so diagnostic and preventive methods are crucial, more over comprehensive national vaccination can help to noticeably reduce the infectious cases caused by the disease.

In this study, the early effects of percutaneous nephrolithotomy (PCNL) on glomerular filtration rate (GFR) was assessed in different postoperative times and likewise, we determined the correlation of different variables with significant postoperative GFR drop after PCNL. Patient records of 486 cases that had undergone PCNL from January 2010 to October 2011 were reviewed retrospectively. GFR in six hours, one, two and three days after PCNL and in the discharge day were calculated and then compared with preoperative level. Correlation between different variables (Perioperative hemorrhage, co-morbidities, previous stone surgery, renal anomaly, number of access, stone burden and location) and risk of acute postoperative renal function impairment (GFR drop greater than 25%) were assessed. Mean preoperative GFR was 87.85±29.41ml/min/1.73m² which decreased to 86.18±28.77, 78.45±28.74, 78.79±26.94, 84.24±29.71 and 86.18±28.77 in 6, 24, 48 and 72 hours after surgery and discharge day post PCNL, respectively. GFR significantly decreased in one and two days after surgery (p value<0.0001 and p value <0.05) but returned to near preoperative values in 3th post PCNL day. Among different variables, only perioperative bleeding (Cut-off point for serum hemoglobin drop was 2.8 mg/dL) was concomitant with significant postoperative renal function impairment. Our findings revealed that co-morbidities, large or multiple stones, multiple punctures and previous history of stone surgery have no significant impact on surgical outcomes. Postoperative GFR returned to near preoperative values in a few days after operation. Avoidance of significant perioperative bleeding is an important point to prevent post PCNL renal insufficiency.

Severe abdominal pain is not common after laparoscopic surgeries, but acute or chronic pain after operation is considerable in some patients. Post-operative Pain control after laparoscopic surgeries, is conventionally achieved using analgesics such as non-steroidal anti-inflammatory drugs (NSAIDs) and narcotics, but their administration has a lot of side effects. This study compares the efficacy and side effects of local anesthetic drugs versus conventional analgesics in post-operative pain control. This prospective investigation was conducted into two groups of patients (n=93). Group 1, as control group, was given conventional analgesics such as narcotics and NSAIDs. In investigational group, at the end of laparoscopic surgery, prior to port withdrawal, a local anesthetic mixture, a short acting (Lidocaine 2%) plus a long acting (Bupivacaine 0.5%) is instilled through the port lumen between the abdominal wall layers. The efficacy of both types of medications was compared to their efficacy and side effects. 85% of the control group, received 5 to 20 ml Morphine for pain control while the others were controlled with trans-rectal NSAIDs. In the treatment group, the pain of 65% of the patients was controlled only by local anesthetic drugs, 30% required NSAIDs and the other 5% required narcotics administration for pain control. The administration of local anesthetic drugs after laparoscopic surgery is an effective method for pain control with a low complications rate and side effects of narcotics.