مجله علوم و فنون دریایی
سال سیزدهم شماره 1 (بهار 1393)

Caviar-producing fish with their economically valuable product are important in fisheries. The cDNA growth hormone (GH) of Sturgeon fish Huso huso was constructed using total RNA from pituitary glands. To construct the recombinant and active lentiviruses carring GH gene، this DNA sequence was inserted into the cloning vector pTZ57R/T and subsequently cutted from pTZ57R/T by endonuclease enzyme and incorporated into lentivirus vector pNL-EGFP/CMV-WPRE on upstream of an IRES cassette. We also insert a reporter EGFP gene downstream of IRES so transfection and transduction steps can be traced. Using this vector plus virus packaging and envelope vectors، HEK-293T cells was co-transfected by DNA-Lipofectamine complex method. Cell supernatant full of virions was collected 48 hours later and concentrated using Amicon columns to obtain a high-titer virus stock. Nearly 1/5 of this stock was applied to a new batch of cultured HEK-293T. After 72h expression of EGFP gene was Detected and the cells was collected for further analysis. Total RNA of these transduced cells was Extracted and GH mRNA expression was revealed by RT-PCR. Results showed that، lentiviral vectors (LV) as a gene transfer system provide efficient delivery، integration and long-term expression by establishing a stable provirus in target cells and could be An important tool in aquaculture and fisheries biotechnology research to increase the growth rate of farmed fish by transferring growth hormone (GH) transgenes into fish.

The properties of kiddi shrimp (Parapenaeopsis stylifera) gel added with different levels of microbial transglutaminase (MTGase) (0. 6، 1، 1. 4 and 1. 8 units/g) were studied. Most textural parameters of the gels improved by adding MTGase espacially in higher levels (1. 8 and 1. 4). The expressible moisture and trichloroacetic acid content declined with the increase in MTGase content. MTGase espacially in higher levels was effective in the color of the gels so that it increased L and decreased a and b. SDS-PAGE study revealed that myosin heavy chain enderwent polymerization to a higher extent in the presence of MTGase. Microstructure of gels added with 1. 8 units of MTGase had more ordered fibrillar structure compared with those of gels without MTGase. Therefore MTGase play an important role in improving the gel properties of kiddi shrimp mince.

The optomotor response is one of the methods used for measuring zebrafish behavior towards moving objects. The present research has been carried out on zebrafish reaction to moving objects. In the experiment، newly fish matured of both sexes were used. To study the optical power of zebrafish an appropriate apparatus was designed. Experiments were performed with different widths of stripes in black، white (1، 2، 7 cm)، with width stripes of 1 cm in red - gray، green - gray at different velocities of 2، 3، 4، 7، 10، 15 rpm. Results of 2-way ANOVA showed significant differences between the various widths، velocities (P<0. 05). Evaluation of results showed that at different widths، fish showed the most optomotor response at a velocity of 10 rpm (0. 65±0. 13)، the lowest response to velocity was shown at 2 rpm (-0. 07±0. 22). Furthermore، the best visibility was observed with 1 cm width (0. 70±0. 13) (P<0. 05). Experiment on color vision also showed that at different velocities، various colored strips، there is no significant difference (P>0. 05). Nevertheless، the fish demonstrated the most optomotor response to green - gray color (0. 10±0. 13)، a velocity of 10 rpm (0. 41±0. 13).

The rivers that pour to seas and oceans are known as tidal rivers. A governing factor on tidal rivers is tidal bores which are produced by the gravity of moon and sun. Reflection of waves from beaches and the shape of beaches and effects of shallow water، effect on tidal bores in the mouth of tidal rivers. These factors convert tidal bores from time periodic condition to no periodic condition. Tidal bores move to upstream of river while fluvial flows go toward downstream of river. Because of combination of Tidal bores and fluvial flows، hydraulic and hydrologic conditions are very complex in the tidal rivers. Measurement of velocity and discharge of current is impossible in the tidal limit of tidal rivers. For determination of stage-discharge curves، an especial method is needed. In this research، water surface elevation is estimated by using discharge of fluvial flow in Ahvaz and tidal height in Khoramshar in tidal limit of the Karun River (from Khoramshar to Darkhovein). Water surface elevation is shown by structural curves and regression relations. These curves and relations are suitable tools for determination of water surface elevation in the tidal limit of the Karun River by using discharge of fluvial flow in Ahvaz and tidal height of Khoramshar.

In this article estimation of storm surge dominated wave height in Iranian coastlines of Oman sea have performed for Geuno storm period، Improved version of a 2D model which produced by Maa in 1990 used in this study (Maa Jerome P. -Y.، 1990). Variant temporal and spatial wind pattern can be used in this model as well as steady wind pattern in risk studies. This paper focused on the Geuno Storm because of wind data accessibility for this period and results compared with satellite data. Efficient results show that this model can be uses for estimate of high of water dominated by storm surge in Oman for other storm events.