فهرست مطالب

Gene, Cell and Tissue - Volume:2 Issue:1, 2015
  • Volume:2 Issue:1, 2015
  • تاریخ انتشار: 1393/12/17
  • تعداد عناوین: 9
|
  • Fatemeh Rostami, Fatemeh Khosravi Moghaddam, Seyed Kazem Sabbagh, Saeide Saeidi Page 2
    Background
    In recent years, die-back of pistachio has become one of the most important diseases in Kerman gardens. With regard to the importance of this disease and the lack of comprehensive information regarding the population genetic structure of the pathogen, it is necessary to set an appropriate indicator in the study of genetic diversity..
    Objectives
    In the present study, we examined simple sequence repeats (SSRs) and restriction fragment length polymorphism (PCR-RFLPs) (two PCR-based marker assays) to determine Paecilomyces variotti genetic diversity..
    Materials And Methods
    The utility of SSRs and PCR-RFLPs was examined to determine genetic diversity using 20 isolates of Paecilomyces variotii. In order to determine the performance of indicators, effective multiplex ratio (EMR), polymorphism information content (PIC), and marker index (MI) were calculated..
    Results
    Both systems discriminated 20 isolates of P. variotii successfully but were different in the amount of detectable polymorphism. Using cluster analysis of digestion reaction, SSR based on UPGMA algorithm, and Jaccard similarity coefficient, the isolates with 70% similarity level were divided into 7 and 3 groups, respectively. Reviewed indicators were at higher level for PCR-RFLPs marker. Four restriction endonucleases enzymes in RFLP produced 20 loci that 90% of them were polymorphic; and for SSR it was 32 loci that 37.5% were polymorphic..
    Conclusions
    This is the first research in comparing two genetic marker systems in P. variotti. We were prompted to explore polymorphisms utility in P. variotti with a look at using germplasm screening mapping of genome and strain improvement programs..
    Keywords: Haplotype, Polymorphism, Pistachio
  • Yadollah Mehrabi, Parvin Sarbakhsh *, Jeanine J. Houwing, Duistermaat, Farid Zayeri, Maryam Sadat Daneshpour Page 3
    Background
    Serum total cholesterol is an established risk factor for coronary heart diseases. In addition to environmental factors and main effects of polymorphisms, genetic interactions can influence cholesterol level. Furthermore, polymorphisms effects can be time-dependent. So, they could be valuable to study of genetic interactions over time..
    Objectives
    In this study we proposed logic mixed model to assess the association of Single-nucleotide polymorphism and other risk factors with longitudinal quantitative cholesterol level with respect to their interactions..
    Materials And Methods
    Data of 329 subjects with age ≥ 20 years that participated in Tehran Lipid and Glucose Study with complete data for three phases of study was analyzed using proposed model..
    Results
    The results showed that the cholesterol level of male or subjects with GG genotype for ApoAIV and normal waist circumstance and normal blood pressure was 19. 8 mg/dL less than other subjects without this combination (β = -19. 8, CI 95%: -13. 9, -25. 69). Also, having «high triglyceride or allele e2 for ApoE» was associated with an increased effect on cholesterol (β = 16. 1, CI 95%: 11. 64, 20. 55). The level of the cholesterol in phase one of study was 21. 4 mg/dL (CI 95%: 18. 35, 24. 44) more than other phases. The variance component of the random effect was statistically differing with zero..
    Conclusions
    In this study, we extended logic regression to the longitudinal quantitative response and applied it to the TLGS data. We identified some interactions among SNPs and other covariates related to cholesterol level using logic mixed model..
    Keywords: Cholesterol, Coronary Disease, Polymorphism, Single Nucleotide, Regression Analysis
  • Mohammad Hashemi *, Majid Naderi, Ebrahim Eskandari Nasab, Seyed Shahaboddin Hasani, Simin Sadeghi Bojd, Mohsen Taheri Page 4
    Background
    The human murine double minute 2 (MDM2), an oncoprotein, is the major negative regulator of P53..
    Objectives
    The purpose of this study was to evaluate the impact of 40-bp insertion/deletion (ins/del) polymorphism in the promoter of MDM2 and vulnerability to childhood acute lymphocytic leukemia (ALL) in a sample of Iranian population..Patients and
    Methods
    This case-control study was performed on 75 children diagnosed with ALL and 115 healthy children. The 40-bp ins/del variant was determined by using the polymerase chain reaction method..
    Results
    Our findings showed that neither the overall chi-square comparison of cases and control subjects (X2 = 1.13, P = 0.569) nor the logistic regression analysis (codominant: OR = 1.29, 95% CI = 0.59-2.14, P = 0.745, ins/del vs. ins/ins; OR = 1.59, 95% CI = 0.59-3.77, P = 0.372, del/del vs. ins/ins, dominant: OR = 1.25, 95% CI = 0.69-2.23, P = 0.552, ins/del + del/del vs. ins/ins and recessive: OR = 1.51, 95% CI = 0.67-3.43, P = 0.395, del/del vs. ins/ins + ins/del) indicated any association between MDM2 ins/del and ALL in our population..
    Conclusions
    Our findings indicated that MDM2 40-bp ins/del polymorphism was not associated with ALL in our Iranian population. Further studies with larger sample sizes and diverse ethnicities are required to verify our findings..
    Keywords: Acute Lymphoblastic Leukemia, Polymorphism (Genetics), Iran
  • Akhilesh Dubey, Mukunda Goswami *, Kamalendra Yadav, Amit Mishra, Ashvini Kumar Page 5
    Background
    Fish cell lines are advantageous alternatives to mammalian cell lines for carrying out in vitro research. They are used extensively for the study of fish biology, physiology and toxicology, and more recently for germplasm conservation of important fish species..
    Objectives
    The present study aims to establish and characterize a novel fish cell line from muscle tissue of Wallago attu catfish, while evaluating its potential as an in vitro system for toxicity testing..
    Materials And Methods
    Explant culturing method was used for the establishment of a cell line in Leibovitz-15 medium with 20% fetal bovine serum (FBS). The resulting cell line was characterized for optimal growth parameters, authenticity, stability, cellular morphology and revival efficiency. The conformity of the cell line for applied studies was established by using gene transfection and cytotoxicity studies against the organophosphate pesticides chlorpyrifos and malathion. Basic fibroblast growth factor was supplemented at 10 ng/mL concentration. The origin of the cell line was authenticated using homology analysis of amplified gene sequences of 16S rRNA and mitochondrial Cytochrome Oxidase Subunit I (COI) gene. Cytotoxicity assessment of the pesticides was assayed by using methylthiazol tetrazolium assay (MTT) and neutral red uptake..
    Results
    The resulting muscle cell line was designated as Wallago attu Muscle (WAM) and was maintained for over 50 passages at optimal growth temperature of 28°C. Chromosomal analysis confirmed the stability of the cell line. The cells exhibited fibroblastic morphology with good transfection and revival efficiencies..
    Conclusions
    The established muscle cell line designated as WAM presented the stability while possessing good transfection and revival efficiency. These characteristics confirmed through cytotoxicity assessment of the pesticides using methylthiazol tetrazolium assay and neutral red uptake support the availability of this cell line as an in vitro system for toxicity evaluation of aquatic pollutants..
    Keywords: Transfection, Pesticides, Cell Culture
  • Zahra Heidari, Hamidreza Mahmoudzade Sagheb, Azam Asemi Rad *, Mahmoud Ali Keikhaee Page 6
    Background
    There is a potential risk for malignancy in patients with diabetes mellitus. Theoretically, a noninvasive method for detection of precancerous changes can be conducted on oral exfoliated cell smears..
    Objectives
    In this study, the immunocytochemistry of p53 in exfoliated oral mucosal cells in patients with type 2 diabetes was investigated..Patients and
    Methods
    Oral mucosal smears were prepared from 50 patients with type 2 diabetes (D) and 50 healthy participants (C). The immunocytochemistry of p53 was assessed according to the kit instructions on oral smears. The Mann-Whitney nonparametric test was used to compare the p53 expression in D and C groups..
    Results
    The results showed a significant increase in p53 expression in exfoliated cells of oral mucosa in D group compared with C group (P < 0.05). There was a significant correlation between the intensity of p53 expression and level of fasting blood sugar (FBS) (r = 0.816, P < 0.001), as well as between the percentage of p53 positive cells and FBS. The nucleus area (NA) and the cytoplasm to nuclear ratio (CNR) had significant correlations with p53 expression (r = 0.272, P = 0.08) NA, and CNR (r = -0.434, P = 0.005)..
    Conclusions
    This study showed that there was a significant expression of p53 in oral mucosal exfoliated cells of group D patients compared with C group. It seems that this noninvasive technique could be useful to detect premalignancy changes of oral mucosa as well as for early detection of malignancies..
    Keywords: Diabetes Mellitus, Immunocytochemistry, Buccal Mucosa, p53 Genes
  • Xiuping Yu, Robert J. Matusik, Renjie Jin * Page 7
  • Fernando Augusto De Lima Marson *, Jose Dirceu Ribeiro, Carmen Silvia Bertuzzo Page 9