مجله به زراعی کشاورزی
سال شانزدهم شماره 4 (زمستان 1393)

Satureja sahendica Bornm. is an endemic medicinal plant which grows wild in natural habitats of west and north-west regions of Iran. In this study، for domestication of Satureja sahendica and study it''s essential oil content and composition، the seeds of seven populations were collected from natural habitats and cultivated in three replications as a complete randomized blocks design. The aerial parts of each accession were collected in full flowering stage at three consecutive years. The essential oils were obtained by hydro-distillation and analyzed by GC and GC/MS. The results showed the highest oil yields were obtained at the first year after cultivation. The major compounds in all oils were Thymol، P-cymene and -terpinene with different percentages. The highest amount of thymol was found in the oils of the plants at second year. In the other words، the oils had better quality، in the second year. The percentage of -terpinene were reduced in the oils in the second year and then increased in the third year. Amount of p-cymene did not show similar changes during three studied years. Generaly، it can be said annual plants had higher oil yields and two-year old plants had better oil quality.

In order to improve the quality and the possibility of fresh pistachio exports, the impact of different harvest time on storage of ‘Ahmadaghaii’ Pistachio was investigated. In 2011, pistachios harvested at three stages of fruit ripening (40, 70 and 100 percent of hulling) in hulling fresh, fresh without hulling and dried pistachio packaged in pulpy bag and held in two-temperature storage (1 ± 4 and 1 ± 12 ° C) for 45 Days. Data were analizaed with factoriel in format of randomized completely design in three replications. The results showed expedition and delay in harvest, increases period of storage and temperature result to increes of endocarp spots and low fruit retention. Increase of temperature and during storage result to decress of taste and low fruit firmness and kernel damage, acidity, peroxide, water loss were increase. This data showed that to keep fruit fresh pistachio, harvesting in stage of 70-80% of ripening, processing with fresh hulling and storage in 1 ± 4 ° C temperature, result to retention of fresh pistachio to 45 days.

In order to evaluate the effect of different mechanical treatments (application of sulphuric acid, boiling water and flooding) on seed dormancy breaking of Licorice, an experiment was carried out in randomized complete block design in 2012. Moreover, a factorial scheme in RCBD was performed to assess the application of giberalic acid and potassium nitrate treatment as well as stratification and rotational temperature/light treatment. Treatments included different levels of giberalic acid (0, 100, 250 and 500 mg) at two time levels of 2 and 24 hour (along with scarification pre-treatment), concentrations of potassium nitrate (0, 50, 100 and 150 mM) in 10 and 30 minutes (along with scarification pre-treatment), stratification temperatures treatment of -5, 0 and 5 °C for one, two and three weeks of treatment, treatment of rotational temperature (5-15 and 10-20 °C), in full light, dark and rotational light periods (8 h light and 16 h dark) and mechanical treatments of dormancy breaking. Treatment duration of two minutes in boiling water and two days water-logging treatment were the most effective treatments on germination percentage of Licorice (81.33% and 53.33% increase in germination percentage compared to control, respectively). Application of giberalic acid and potassium nitrate treatments and stratification had no effect on Licorice dormancy. According to the Licorice seed dormancy breaking in mechanical treatments and the effect of rotational temperature and light, it can be concluded that the dormancy of Licorice seeds is intermittent.

To determine the effect of farm slopes on yield and some agronomic traits of wheat under different fertilizer treatments, an experiment was performed as split plot based on randomized complete block design with three replications in Piranshahr in 2011-2012. Treatments consisted of farm slope at four levels: (zero, 3, 6 and 10 %) as the main plots and combination of different fertilizer at five levels: (control, application of biofertiliozer (Nitroxin), manure, chemicals (N and P) and integration of biological, manure and chemical as sub plots. Results showed that the highest (7.67 t/ha) and lowest (3.68 t/ha) seed yield obtained from farm slope 0 and 10%, respectively. In fact, with increasing of the farm slope, the yield components decreased which lead to 52% and 38% reduction in grain yield and total biomass of plants located on a slope of 10 percent than the control plants. Also, the applications of integrated fertilizer treatments had the most effect on morphological traits and yield components of wheat that increased 12.58 and 13.58 percent the seed yield and total biomass compared to the control plants.

This experiment was conducted in Safi Abad Agricultural Research Center of Dezful in order to study the effect of seed deterioration and plant density on germination, seedling establishment, yield and yield components of hull-less barley with in 2011- 2012. Treatments included three levels of seed deterioration: (control, 72 and 96 hours) and five plant densities (100, 150, 200, 250 and 300 plants per m2). Results showed that the seed deterioration significantly affected variables of 1000 grain weight, spikes number per m2, fiber percentage, protein percentage, biological yield, grain yield and harvest index (HI) was significant. Further more, plant density significantly affected spike number per m2, 1000 grain weight, grain yield, harvest index, fiber percentage, protein percentage and biological yield. It can be concluded that use high quality of seeds, in addition to the plants during growth and development, result in yield increase.

In this study the effects of source limitation on grain yield of twelve barley cultivars (Aras’,‘Jonub’,‘Reihan’,‘Zarjou’,‘Sararoud’,‘Sahra’,‘Fajr30’,‘Karoun’,‘Gorgan’,‘Makouei ’and ‘Nosrat(’ under post anthesis water deficiency were studied. Source limitation treatments were included that control, removing of awn, leaves below flag leaf, flag leaf and spike photosynthesis. All source limitation treatments significantly reduced grain yield, 1000 grain weight and number of grains per spike. In non-water stress, the highest reduction of grain yield was seen in removing of leaves below flag leaf treatment (27.6%). In water deficiency, the highest and the lowest grain yield reduction were seen in removing of spike photosynthesis (32%) and removing of awn (18.1%). Flag leaf removing significantly increased photosynthesis rate in remaining leaves in non-water stress (28.8%) and water deficiency (10.8%). The evidence from this study suggests that under water deficiency after anthesis, spike photosynthesis is more important than the other current photosynthesis organs in grain yield formation in barley.

Modern biotechnology and genetic engineering using techniques and special ‎laboratory tools plays an important role in the pharmaceutical industry and medicinal ‎plant propagation. Among these techniques, it can be noted that a wide range of ‎elicitors are widely used for inducing metabolites at invitro culture. Due to the ‎pharmaceutical properties of artichoke and its history, it has been deeply noticed by ‎pharmaceutical industry and its cultivation is expanding rapidly. In present study the ‎effect of two elicitor's methyl jasmonate (MJ) and salicylic acid (SA) on the callus ‎mass growth and survival of invitro culture was investigated. Callus cultures of ‎artichoke were established by transferring seedling on solidified MS medium ‎supplemented with different concentrations of salicylic acid and methyl jasmonate. ‎Four weeks after subculture, the callus was collected and weighed. Results showed ‎that the SA supplementation reduced fresh weight of callus. Contrary to fresh weight, ‎a direct increase was observed in dray weight by increasing the level of SA. Opposite ‎to SA different concentration of MJ not only did not changed the fresh weight, but ‎also reduced the dry weight of callus. Study the pigment changes under different ‎treatments of SA and MJ showed that, contrary to cartonoid a direct reduction was ‎observed in chlorophyll content by increasing in both SA and MJ concentration. ‎Finally it can be suggested that, however both SA and MJ as most important elicitors ‎increase secondary metabolite production of callus, their availability in high ‎concentration affect the cell grow activity, callus survival and the biomass production.‎

In order to study of Sodium Nitroprusside(SNP) application effect on growth indices and water use efficiency of kidney beans cultivars(Akhtar and Derakhshan) under water deficit stress, an experiment was conducted in split factorial based in randomized complete block design with three replications in Bu-Ali Sina university research field at 2012. Water stress treatments included vegetative stress, reproductive stress, vegetative+reproductive stress and non-water stress (control). Foliar application of SNP was done in 3 concentration(0, 150 and 300 µM SNP). Reproductive stress reduced leaf area, crop growth rate and total dry weight in both cultivars. SNP foliar application (300 µM) increased mentioned indices 28, 26 and 20 percent in Akhtar and 28, 20 and 20 percent in Derakhshan in comparison to without foliar application, respectively. Water use efficiency of grain and water use efficiency of biomass in vegetative stress was more than of non-water stress. Maximum amount of water use efficiency was for Akhtar cultivar with 300 µM Sodium Nitroprusside foliar application and were 0.72 and 2.9 percent for grain and biomass, respectively. SNP foliar application ameliorated and improved negative effects of vegetative and reproductive stress. SNP application(300 µM) had more effect on growth and yield in both cultivars at all water regimes. Therefore, foliar SNP(300 µM) it’s desirable for kidney bean under water deficit stress.

This investigation was carried out in a commercial orchard in Ashtian city, Markazi province. The effect of N-phenyl-phthalamic acid and nutrition on fruit quantity and quality of plum cv. Maragheh was studied with the aim of increasing yield. The N-phenyl-phthalamic treatments (concentrations, 0, 500 and 1000 mg/l) were performed at full bloom (80 % flowering) on 29 March. Also, nutrition was conducted on 18 May using perfect liquid fertilizer (Plant Green). As a result of this process, the yield amount was increased, when N-phenyl-phthalamic acid 500 and 1000 mg/l treatment were used without fertilization comparison to control. But fruit quality parameters (weight, length, and width) were decreased in comparison to control. However, treatments N-phenyl-phthalamic acid 500 and 1000 mg/l with fertilization not only increased fruit setting but also increased fruit quality characters (weight, length, and width). According to the present results, N-phenyl-phthalamic acid (500-1000 mg/l) along nutrition can be used for the improvement of fruit quantity and quality of plum.

This study was designed to investigate the effect of P. fluorescens FY32 inoculation on the activities of some antioxidant enzymes and ion concentration of two canola cultivars (Sarigol and Hyola308) under salinity stress (150 and 300 mM NaCl) in the greenhouse condition (fall 2012، Dept. of Plant breeding and Biotechnology، Faculty of Agriculture، University of Tabriz). The experimental design consisted of three factors replicated three times in a split-split plot design، with salinity as main factor. Data analysis showed that salt stress has significant effect on antioxidant enzyme activity (catalase، peroxidase and polyphenol oxidase). Concentration of sodium and chloride ions increased with increasing salinity، but this increase was little in the case of inoculated plants. The decrease in potassium content was smaller in inoculated plants in comparison with control. Hyola308 had the lowest reduction in dry matter and was tolerant cultivar in this experiment. Thia cultivar as a tolerant cultivar had low level of antioxidant activities and accumulation of sodium and chloride ions and also showed high concentration of potassium.

In order to determine the critical period of weed control(CPWC) in Corn(Zea mays L.), an experiment was carried out in a randomized complete blocks design with 14 treatments and three replications during 2013 growing season in the Nahavand climatic conditions. Two series of the treatments including weed-free and weed-infested were applied in the regular time distances of 10, 20, 30, 40, 50 and 60 days after Corn emergence. Two check treatments including full and no control of weed during the whole season were also included. The nonlinear models kind of Logistic and Gompertz were applied to determine the start and end of critical period of weed control respectively. Major weed species were field bindweed(Convolvulus arvensis L.), red root pigweed(Amaranthus retroflexus L.), pigweed(Amaranthus blitoides L.) and lambsquarters(Chenopodium album L.) with greater biomass and size. The results showed that periods of weed- free and weed- infested differently affected the dry weight and number of weeds in all treatments. The critical periods of weed control in Corn by accepting a 5% acceptable yield loss, it is necessary to control weeds in a period between 14-47 days after planting or 117- 566 growth degree day(4-12 leaf stage) and by accepting a 10% acceptable yield loss it is necessary to control weeds in a period between 23-36 days after planting or 220- 419 growth degree day(6-10 leaf stage).

In order to study of use advantage in the intercropping system fennel (Foeniculum vulgare Mill) and fenugreek (Trigonella foenum-graecum L) in different ratios of intercropping and sole cropping, an experiment with use replacement and additive intercropping based on randomized complete block design with three replications was conducted at the Research Station of Faculty of Agriculture, University of Zanjan, Iran, during growing season 2012. Treatments included additive intercropping in three levels (100 percent fennel + 33, 66 and 100 percent fenugreek), replacement intercropping in six levels (1:1, 1:2, 2:1, 2:2, 1:3, 3:1), Sole fennel and Sole fenugreek. Results showed that the highest grain yield of Fenugreek was obtained in pure stand of Fenugreek and the lowest of mentioned trait was obtained in the 75 percent fennel + 25 percent fenugreek (3:1) replacement series. Also the highest and the lowest fennel grain yield were obtained in 100 percent fennel + 33 percent fenugreek additive series and 25 percent fennel + 75 percent fenugreek (1:3) replacement series respectively. yield of essential oil at 100 percent fennel + 33 percent fenugreek additive series were higher compared with sole cropping. The highest Land Equivalent and Relative Value Total obtained from 100 percent fennel + 33 percent fenugreek additive series. The highest Actual Yield Loos or gain (AYL) and intercropping advantage (IA) obtained from 25 percent fennel + 75 percent fenugreek (1:3) replacement series. Aggressively index revealed that in most sowing ratios fennel was dominant and fenugreek was defeated.

In order to evaluate the effects of drought stress and planting method on some physiological and biochemical characteristics of two chickpea cultivars (Cicer arietinum L.) an experiment was conducted at the research station of College of Agriculture, Shiraz University using a split factorial based on randomized complete block design in four replications. The main factor was stopping irrigation at different growth stages of chickpea (full irrigation=I1, flowering=I2 and two weeks after emergence =I3) and the sub-factor included a combination of pea varieties (Arman and Azad) and sowing methods (planting on ridges, furrow and basin), respectively. Results showed that under drought stress, leaf area index, stomatal conductance, photosynthetic rate, chlorophyll a and b and total chlorophyll content decreased while proline content increased. The cultivar Azad had higher than to Arman, leaf area index, stomatal conductance, photosynthesis rate and chlorophyll. The highest photosynthesis rate obtained under full irrigation treatment (16.09µmol m-2s-1) and it’s reduced to 25.79 and 14.23 percent under water stress in I3 and I2 treatments respectively. Based on these results, it can be concluded that the cultivar of Azad is suitable for this region and it is suggested to be planted on the ridge as has higher photosynthesis rate and lower stomatal conductance reduction leading to a higher yield.

A field experiment was conducted to evaluate the effect of varying doses of haloxifhope-R-methyl ester herbicide on grass weeds grown at various stages in 2011-2012 growing season in Research Farm of College of Agriculture, Shiraz University. The study was carried out as split split plot based on randomized complete block design with three replicates. Treatments were haloxifhope-R-methyl ester herbicide doses (0.6, 0.8 and 1.0 L/ ha) as main factor, surfactant (with and without) as sub factor and time of herbicide application (2 leaf, 6 leaf and tillering of grass weeds) as sub sub factor. A check treatment (weedy) was also included. A rise of herbicide dose, decreased wild oat and foxtail dry weight 35.7 and 12.6 gr/m2, respectively. The results showed that the delay in herbicide application and reduction of herbicide dose decreased grain yield and its components. The highest reduction in grain yield was obtained with 0.6 L/ ha haloxifhope-R-methyl ester herbicide at tillering stage. Application of surfactant caused a decrease in weed dry weight compared to no surfactant and also increased grain yield significantly. In general, to achieve maximum grain yield and the best grass weed control, application of 1 L/ ha haloxifhope-R-methyl ester with surfactant use at 2-leaf in safflower field is recommended.

This experiment was carried out to evaluate the effect of Rhizobium strains and plant growth promoting Rhizobacteria(PGPR) on yield and yield components of chickpea(Cicer arientinum L.) Arman cultivar. This research was conducted as split plot based on randomized complete block design at Zanjan university researchal farm. Irrigation levels(optimum irrigation during season growth and no irrigation during growth period) were set as main plots and fertilizer level(control or no application chemical and biological fertilizer, 50kg/ha Urea, seed inoculation with Mesorhizobium ciceri strain of SWRI-3,seed inoculation with Mesorhizobium ciceri strain SWRI-17, seed inoculation with PGPR,Co-inoculation with rhizobium strains SWRI-3+SWRI-17 and Co-inoculation with all biofertilizer, PGPR+SWRI-3+SWRI-17) were sets as subplots. The results of this study showed that drought stress reduced plant height, number of branches, number of seeds per plant, seed weight, biological yield and harvest index.Applicatin of biofertilizers because of positive effects on plant height, number of seeds per plant, seed weight, pod weight per plant and biological yield,produced more grain yield in comparison control and nitrogen urea fertilizer in both of arrigated and rainfed conditions.In addition, seed co-inoculation with all of bio-fertilizers(PGPR+SWRI-3+SWRI-17) achieved more grain yield in comparison separate inoculation about 18-36% in irrigated and 34-50% in rainfed conditions.

In order to investigate the yield and quality of forage in intercropping barley and fennel, an experiment was carried out as split plot based on a randomized complete block design with three replications at the research farm of Agriculture and Natural Resources of Ramin University during growing season of 2012-2013. Four treatments levels of nitrogen including: (0, 70, 140 and 210 kgN.ha-1) have been taken into account as main plots and the planting ratios in five levels (pure culture barley and fennel), (75% barley + 25% fennel), (50% barley + 50% fennel), (25% barley + 75% fennel) as sub-plots. Results showed that the highest dry and fresh forage yield, respectively, 53380 and 8271 kgN.ha-1 were obtained from treatments pure cultures of fennel with level of 210 kgN.ha-1 and 50percent barley + 50percent fennel with level of 210 kgN.ha-1. Morphological traits included height of barley and fennel, number of branches for fennel were positive affected nitrogen 140 kg.The highest percent crude protein with 25.7 percent was obtained from pure cultures of fennel with 210 kgN.ha-1. Also, maximum crude protein yield (1528.2 kgN.ha-1) and land equivalent ratio (LER=1.16) were obtained from 50percent barley+50percent fennel with level of 210 kgN.ha-1 respectively. The authenticator results of aforesaid inspections enhance the forage yield and quality in treatment 50 percent barley+50percent fennel with level of 210 kg N.ha-1

To evaluate yield and productivity indices of maize and sugar beet intercropping with different planting ratios, a field experiment was conducted in randomized complete block design with three replication at Hamedan province in Nahavand, in 2013. The planting ratios were 100:0, 75:25, 50:50, 25:75 and 0:100 (maiz: sugar beet) using replacement method. Results showed that intercropping had significant effect on yield and some yield component of both crops. The root-yield, sugar sterling, melas-sugar, content purity and sugar yield of sugar beet as well as shoot height, No. row. ear-1, kernel. Row-1, 1000 kernel weight and grain yield of maize were significantly affected by different planting ratios. Calculation of land equivalent ratio (LER) revealed that planting ratio of 25:75 (LER= 1.09) and 75:25 (maize:sugar beet) (LER= 1.08) had the highest efficiency by 9% and 8%, respectively. Calculation of area time equivalent ratio (ATER) revealed that planting ratio of 25:75 (maize:sugar beet) (ATER= 1.03) had the highes efficiency. Maize in the planting ratio of 75:25 had the highest relative crowding coefficient (ka= 7) as dominant crop and sugar beet had the highest RCC (KB= 3.88) in the ratio of 25:75 (maize:sugar beet) at the dominant crop.

Screening of native cultivars and understanding of the mechanisms involved in cold hardiness in grapevines is needed to match cultivars appropriately with growing sites and aid in breeding and selecting cultivars with improved freezing tolerance. In this study cold hardiness of fifteen grapevine (Vitis vinifera L.) cultivars were evaluated using tetrazolium stain test and post freezing budbreak assays during six months from acclimation till deacclimation stages. Moreover, seasonal changes pattern in soluble proteins, total phenol and Malondialdehyde (MDA) of buds were measured during this period. Significant differences (P≤0.01) were found among cold hardiness of cultivars in all stages. ‘Bidaneh Ghermez’ and ‘Khalili’ (LT50= -22 and -22.6 ºC in respectively) were the hardiest ‘Ruby’ and ‘Perlette’ (LT50= -16.1 and –16.9 ºC in respectively) were the least hardy cultivars in January. Soluble proteins and total phenol concentrations of buds increased during cold acclimation from November to January then decreased in March. The concentration of these compounds was higher in cold hardy such as ‘Bidaneh Ghermez’ and ‘Khalili’ cultivars than least hardy ones, indicating the positive relationship of these compounds with freezing tolerance. The measured MDA in onset and end of dormancy period was lower than its concentration in deep dormancy in January. Lower MDA concentration was found with cold hardy cultivars ‘Khalili’ and ‘Bidane Ghermez’ in comparison with cold sensitive cultivars, ‘Perlette’ and ‘Yaquti’. Our results indicated that high accumulation of soluble proteins and total phenol in cold hardy cultivars resulted in improved membrane stability and freezing tolerance.

In this study, the effect of foliar spray with calcium chloride (0, 0.75, 1.5 and 3 g L-1) during 2, 4 and 6 weeks before commercial harvesting time of apple fruit cv. ‘Gala’ and ‘Delbarestival’ which trained in three different systems (V shape, HighTech and Cordon) was investigated. The results showed that types of training systems and foliar application of calcium can affect on fruits quality at harvest time. Tree yield, fruits weigh and fruit tissue firmness of ‘Gala’ was significantly higher than ‘Delbarestival’. Furthermore, calcium spray increased fruit firmness in compared to control and TA deacresead in compared to control. Calcium treatment increased fruit skin a* value that means more antocyanin synthesis and redness. Results generally showed that ‘Gala’ apple trained in HighTech and V shape systems as well as ‘Delbarestival’ in HighTech produced the higher fruits weight and size as well as quality favorite in compare to other system.

Nowadays, establishment and maintenance of turfgrasses due to high water requirements was faced with serious doubts. Hence, screening of drought resistance native grasses, and introduces them as turfgrass is not only effective in reduction of water usage but can also preserve plant gene pools of the country. In the present experiment, Wheat grass and Bromus as two native grasses were compared with perennial ryegrass as control. After establishment and coverage of the pots surfaces by turfgrass in outdoor, three mowing heights including 2, 4 and 6 cm were applied on the species weekly. During the experiment, shoot growth, tillering, leaf color and leaf width, fresh and dry weight were measured weekly. The results of this research showed that Bromus due to undesirable tissue (leaf width more than 3 mm) and low density is not suitable for use in landscape. Wheat grass despite of rougher texture and less color than perennial ryegrass, has higher tillering, suitable color (7.59) and good texture (leaf width about 2 mm). Also, mowing height of 2 cm was determined as the best for three species studied.