فهرست مطالب
International Journal of Medical Laboratory
Volume:2 Issue: 1, May 2015
- تاریخ انتشار: 1394/03/02
- تعداد عناوین: 9
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Pages 1-15Nanotechnology has opened a new field in medicine as well as in other sciences. The aim of this study was to seek the capability of nanotechnology for the treatment of various reproductive diseases. In this study, we analyzed all articles about “nanotechnology and reproductive medicine” published in 2000-2015, indexed in Google Scholar, PubMed and Science Direct. This study indicated that nanotechnology has been extensively used for different biomedical applications, e.g. detection, drug delivery, diagnostic imaging, etc. particularly in cancer diagnostics and treatment. Here, the emerging uses of nanotechnology in reproductive medicine and reproductive biology were found. The available evidence regarding the use of nanomaterials as experimental tools for the detection and treatment of reproductive diseases was summarized. Nanoparticles have potential applications in reproductive biology. Treatment and imaging of reproductive system-related cancers can be performed by engineered nanoparticles. Also, some non-cancerous diseases can be treated by nanotechnology, e.g. endometriosis. The benefits and concerns associated with their use in a highly delicate system of reproductive tissues and gametes have been investigated. Nano-based methods are innovative and potentially controversial approaches in the clinical settings and give us the mechanisms underlying reproductive diseases.Keywords: Drug Delivery Nanomaterials Nanoparticles Nanotechnology Reproductive Biology Reproductive Medicine
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Pages 16-20IntroductionThe aim of this research was to compare active and passive recovery after a session of intense exhaustive training on the level of serotonin in the serum of the runners.Materials And MethodsIn this semi-experimental study, 26 male elite runners were randomly assigned to two groups of active(n=12) and passive(n=14) recovery. Half an hour before the start of the training, 5 ml blood was drawn from the sample and then each subject was tested starting training on treadmill for Bruce test until reaching exhaustion. Immediately and then 10 minutes after the Bruce test, 5 ml of blood was drawn again for measuring serotonin. Results andConclusionsThe mean of serotonin in three steps of before, immediately after and 10 minutes after recovery were respectively 360.83 ± 250.05, 459.67±302.13, 514.5±440.48 ng/ml in the active recovery group and 141.26 ± 92.41, 241.04 ± 104.78, 214.34 ± 120.03 ng/ml in the passive recovery group. There was a significant difference between the amount of serotonin after recovery compared to that of before. However, the type of recovery program after that had no effect on the serum serotonin of the blood of the runners.Keywords: Active Recovery Bruce Test Passive Recovery Serotonin
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Pages 21-24IntroductionIt is believed that uremia causes destruction of white blood cells (WBC) and thus causes leukopenia. Therefore this study had an attempt to assess the effect of uremia on WBC count.Materials And MethodsThis case control study was conducted on 120 uremic patients and 100 samples as control group. All samples were examined for determination of urea and creatinine in their serum and complete blood counts were determined. Results andConclusionIn healthy individuals, the mean value of urea was 14.5±1.9 mg/dL and the mean value of creatinine was 0.9±0.2 mg/dL (male) and 0.66±3.2 mg/dL (female). In the patient group, the mean value of urea was 83±2.4 mg/dL. The mean value of creatinin in male and female were 2.4±1.3 mg/dL and 2.1±1.7 mg/dL respectively. The mean of WBC count in case and control groups were 6.08± 2.24 and 6.17± 2.43x109/L respectively (p=0.71). Our results indicate that uremia cannot change leukocyte count.Keywords: Leukopenia Uremia White blood cells
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Pages 25-33Background And AimsRecent evidence suggests that T helper (Th) 17 cells are involved in the emergence of asthma. Th17 cells have a key role in inducing inflammation in asthmatic airways thus Interleukin (IL)-17A, the main cytokine of Th17, contributes to airways inflammation.Materials And MethodsWe evaluated the level of IL-17A and total immunoglobulin E (IgE) in sera of 100 asthmatic patients and 81 healthy controls by ELISA to determine how serum concentration of IL-17A is associated with asthma severity. We classified patients into three groups; mild (n=28), moderate (n=33) and severe cases (n=39).ResultsRespectively, serum IL-17A and IgE concentrations were significantly higher in the asthmatic patients than the control group (p=0.026 and p<0.01). Mean of serum IL-17A and IgE values were 37.73 pg/ml and 39.02 IU/ml in the control group and 68.55 pg/ml and 295.87 IU/ml in the patients group. Nevertheless there were non-significant differences between the three groups of asthmatic patients. Respectively, mean of serum IL-17A and IgE values were 94.17 pg/ml and 255.07 IU/ml in the mild group, 71.29 pg/ml and 271.27 IU/ml in the moderate group, and 47.85 pg/ml and 345.97 IU/ml in the severe group. Moreover, there was no correlation between serum levels of IL-17A and IgE.ConclusionIn this study we found that IL-17A, like IgE, rises in sera of asthmatic patients though in a different manner. IgE increases in serum consistent with disease severity though the increases of IL-17A in serum has an inverse relationship with IgE rising.Keywords: Allergy Asthma IgE IL, 17A Th17
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Pages 34-40Background And AimsMultiple myeloma is a malignant proliferation of plasma cells derived from a single clone. The tumor, its products and the host response lead to organ damages. Some factors that are responsible in its pathogenesis are recognized. As FMS like Tyrosine Kinase 3 receptor (FLT3) mutation has been proved as a determining factor in leukemic patients; the goal of this study was to find association of FLT3 internal tandem duplication (ITD) and FLT3 tyrosine kinase domain (TKD), mutations with multiple myeloma.Materials And MethodsThis case-control study was conducted on 60 paraffin-embedded bone marrow biopsies (30 multiple myeloma and 30 normal bone marrow specimens) in the pathology departments of Ghaem and Imam Reza hospitals in Mashhad. After sections preparation, DNA was extracted and two PCR reactions were set up for detection of FLT3/ ITD and FLT3/TKD mutations.ResultsThe Mean age of samples was 64±10 years. No FLT3 mutations were detected in multiple myeloma patients.ConclusionOur findings showed that FLT3 mutations occurrence seem unusual in multiple myeloma.Keywords: FLT3 Multiple Myeloma Polymerase chain Reaction
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Pages 41-49Background And AimsType and saturation of fatty acids can have an important impact on the level of triglyceride, cholesterol, very low and low-density lipoproteins in the blood and thus affect the development of atherosclerosis. Saturated fatty acids have an additive effect on blood cholesterol while for unsaturated fatty acids, a lowering effect has been reported. Fatty acids can have different effects on lipoproteins metabolism and apolipoproteins expression because oils used by human have different compositions. One of the important apolipoprotein is apolipoproteinB (apoB). This study was conducted to compare the effect of different nutritious fats on expression of apoB protein.Materials And MethodsFor this purpose, 48 Syrian male mice were selected and randomly divided into six groups of eight: chow, diet with10%corn oil, diet with10% olive oil, diet with10% cow ghee), diet with10% sheep ghee, and diet with 2% cholesterol. After two months, liver and intestine were removed and transferred into liquid nitrogen and were frozen at -70ºC. Protein was extracted and the expression of apoB was studied by western blotting.ResultsAn increase in the expression of intestinal apoB48 was identified in olive oil and cow ghee groups. Hepatic apoB100 expression increased in the cholesterol group compared with the corn oil group.ConclusionThis study indicates that olive oil and cow ghee consumption increase intestinal apoB48 expression.Keywords: ApoB Cholesterol Cornoil Cow ghee Olive oil Sheep ghee
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Pages 50-57Background And AimsThe immune system protects the body against many invasive foreign materials. The aim of this study was to investigate the effect of an intense anaerobic exercise session on serum IgG, IgM and IgA levels in handballists, volleybalists and climbists.Materials And MethodsIn this study, 45 professional athletes with the average age of 20-30 years old who had participated in the major leagues and the first batch of Zahedan city were invited for this purpose. In this research, 30 second Wingate test was used to assess anaerobic power. Blood samples before, immediately after, and 2h after exercises were collected and serum levels of immunoglobulins IgG, IgM ˛ IgA concentration were measured by nephlometry method.ResultsThe results of this study indicated that the level of serum immunoglobulins IgG, IgM ˛ IgA concentration in all three study groups before and after and two hours past exercise did not significant change (P>0.05).ConclusionOur findings showed that short anaerobic.Keywords: Anaerobic exercise Climbing Handball Immunoglobulin Volleyball
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Pages 58-64Background And AimsHuman Immunodeficiency virus (HIV) is spreading rapidly among the people worldwide. Infection with this virus leads to immune suppression and finally acquired immune deficiency syndrome. Early HIV detection is dependent on antibody screening against virus by Enzyme Linked Immunosorbent Assay (ELISA). Some confirmatory tests such as Western Blot and Recombinant Immunobloting Assay (RIBA) are used to verify viral infection. Many of the confirmatory test results are indeterminate. The aim of this study was to compare the frequency and patterns of indeterminate results of confirmatory tests in two groups; blood donors and patients with high risk behaviors in the northeast of Iran.Material And MethodIt is a cross-sectional study from October 2009 to March 2014, a total number of 1055 serum samples with previous positive HIV ElISA test history were tested in our laboratory, Some by RIBA and some by western blot methodResultsMost of the indeterminate results belonged to blood donors and Western Blot analysis. The most reacting band was P24 in both methods and groupsConclusionRIBA assay is more sensitive and reliable than western Blot but it’s necessary to use other supplementary tests with less indistinctive results. It’s necessary to pay attention to HIV glycoprotein reactivity in some methods too.Keywords: HIV Indeterminate RIBA Western blot
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Pages 65-72Background And AimsEcstasy or 3, 4-methylenedioxymethamphetamine (MDMA) is a brain stimulant and a hallucinogenic material prepared by chemical changes in amphetamine. The aim of this study was to evaluate the changes induced by this drug in mouse cardiac histopathology, electrocardiogram (ECG) and blood cell counts.Materials And MethodsIn this experiment, 3 groups (n=10) of mice were enrolled. Group 1, as control, received placebo. Group 2 mice were given single daily low dose (20 mg/kg/d for 28 days) of intraperitoneal MDMA, and group 3 were given single daily high dose (40 mg/kg/d for 28 days) of intraperitoneal MDMA. An ECG, aVF lead record was obtained, and then a blood sample was taken for complete blood counts and the heart was removed for microscopic study of tissue sections with routine staining.ResultsThe group 3 showed significant decrease in erythrocyte indices, myocarditis in 7 cases and monocyte infiltration around cardiac myocytes in 6 cases. In group 2, lower degree of myocardial injury was observed, displaying significant increase in QT and QTc durations in ECG. In high dose group, red blood count, hematocrit, mean cell volume and mean corpuscular hemoglobin concentration showed significant changes with comparison control group.ConclusionEcstasy can effect on red blood cell index and can lead to anemia. Many monocytes see around of cardiac cell with increase of ventricular depolarization and repolarization can lead to increase of QRS-QT interval. Combination myocarditis with arythmia and increase of sinus tachycardia show change in cardiac function and myocardial structure, cardiac injury due to hypoxia and ischemic can cause of myocardial infarction.Keywords: Blood Count Ecstasy Electrocardiography Methamphetamine