مجله دانشگاه علوم پزشکی اراک
سال هجدهم شماره 4 (پیاپی 97، تیر 1394)

Primary dysmenorrhea is one of the most common complaints in women medicine, in the absence of any pelvic pathological failure. Exercise is commonly reported as a factor to decrease dysmenorrhea symptoms. One of the new therapeutic methods is “Kinesio Taping” which claimed to be able to reduce pain, swelling and muscle spasms. Thus, this study tries to determine and compare the effectiveness of using two therapeutic methods of stretching exercises and taping in painful menstruation of high school students. This study is an operational, quasi-experimental and two- stage study with a control group and pre-test and post-test design. Thirty female high school students with the average age of 16.8±0.99 were selected as available, and were divided into three groups, randomly. For the first group, stretching exercises were carried out and the second group were taped with Kinesio Tape and control group did not any exercise. Mood Disorder Questionnaire (MDQ) was used to collect information. Visual Analogue Scale (VAS) and schober test were used for measuring the level of pain and range of motion, respectively. Data analysis was performed by a repeated measures ANOVA at the significant level of p>0/05. There was a significant improvement in back and abdomen pain and back range of motion.On the other hand, comparing data after intervention showed a significant difference in pain intensity between two groups. But, no significant difference was notified in increased back range of motion in group with kinesion tape. Kinesio Taping has better effects than stretching exercises in reducing tesucing pain. Too, Stretching exercise has better effects in increasing back rang of motion.

Delayed muscular soreness after resistance exercises or eccentric trainings is probably because of muscle damage and injury. Nutrition by playing a crucial role in both protein synthesize and catabolism can influence the extent of muscle injury. The objective of this study was to assess the effect of single portion of Glutamine supplement consumption on injury indices of muscle after a session eccentric resistance exercise. this study used a randomized, double blind design that consisted of 80 volvnteer non-athletic males (aged 22.2±2.2years, height 175±5 cm, weight 71/64±9 kg, body mass index 23/2±2/2 kg/m2, and body fat 17/5±2/4%). A total of 40 participants were divided randomly into 2 groups, supplement group (receiving 0/1 g/kg Body weight/ day Glutamine) and placebo group (receiving 0/1 g/kg Body weight/ day Maltodextrin). Serum keratine kinase (CK) was determined by photometric method, muscle pain and knee joint range of motion were measured using, respectively, a standard scale of PAS and goniometer before, 24 and 48 hours after a resistance test involving knee flexion. Glutamine supplement consumption caused no significant differences in CK levels reduction in none of the measured times, but it reduced the muscle pain at the times of 24 and 48 hours in comparison with the placebo group. In addition, the knee joint range of motion was significantly improved at 24 hours after the test. It seems that this dose of Glutamine supplementation can reduce the apparent signs apart from muscle injury indices reduction.

Birthweight less than 2500 grams is the major indicators of neonatal and infant health. The studies on animals show that copper and zinc deficiency in pregnancy is associated with fetal growth and congenital abnormalities. Therefore, our study was conducted to evaluate the role of these two elements on human embryos. In this descriptive study, cord blood of all infants born in Nik-Nafs Maternity of Rafsanjan was Collected for 1 year. Then, serum was separated and saved. In total, 64 samples including 9 infants with congenital anomalies, 21 infants with a weight equal to or less than 2500 grams and 34 healthy infants were selected randomly as control group. The level of copper and zinc in cord boold was measured by the spectrophotometric method and data analysis was carried out by SPSS 17 Software and statistical methods involving Chi-square, T-test and analysis of variznce. No significant relation was shown between the levels of copper and zinc withbirth weight and congenital abnormalities. Also, there was not any significant correlation between these two teo elements and some pregnancy risk factors such as type of delivery, premature rupture of membrane, placental decolman, placental previa, preeclampsia, gravidity, height, head circumference and Apgar scores. The findings indicate that the levels of copper and zinc in cord blood are not associated with incidence of low birth weight, congenital malformations and pregnancy risk factors.

Body Mass Index (BMI) is commonly used to determine overweightness and obesity in epidemiological studies regardless of the sex and age of the subjects. Golden Standards were presented by the World Health Organization to estimate obesity by measuring body fat percentiles. The primary purpose of this study was to estimate the accuracy of the BMI by Cut-points of gold standard. This is a cross-sectional and descriptive study. Measuring body composition was done by Bioelectrical Impedance Analysis (BIA) method. Accuracy of the BIA method is documented in comparison with more complicated methods Diagnosis performance to estimate the accuracy of Body Mass Index was based on measuring specificity, sensitivity, percentile of the power of positive anticipation, and percentile of the power of negative anticipation to diagnose obesity, with due attention to the cut-points of world health organization gold standards and according to sex and age ranking. Data analysis was performed by T-test, Chi-Square and Roc curve. Two Roc curves were compared by Honely formule and regression analysis. According to the cut-point of gold standard, statistical findings showed that the amounts of sensitivity and specifity were 66% and 90.5% for all persons, respectively. With due attention to data analysis, the level of sensitivity was 50.2% to 73.3% in males and 28.9% to 69.7% in females. Also, with respect to age ranking, percentile of the power of negatire anticipation was reported between 26.7% to 63.6% in males and between 28.9% to 69.7% in females. Body Mass Index could not accurately classify people in obesity and overweightness groups. The power of BMI for classifying obese and overweight people decreases with increasing age. Overall, comparing BMI and the cut-points of gold standard showed the medium effect of this index in classifying obese and overweight persons. The appropriate cut-point to diagnose obesity was 27.8.

Gentamicin is an aminoglycoside antibiotic that broadly is used to treat gram negative bacteria infections, although it has side effects such as nephrotoxicity. According to antioxidant, anti-inflammatory and vasodilatory properties of Zataria Multiflora, the effects of co-treatment with zataria Multiflora and hydroalcholic extract on gentamicin induced nephrotoxicitj were investigated. In this study, male rats of Vistar race were divided into 4 groups: 1.control group, 2. co-treatment with gentamicin and vehicle group, 3. co-treatment with gentamicin and zataria Multifiora extract group, 4. co-treatment with zataria Multiflora extract and normal saline solution group. Zataria Multiflora hydroalcoholic extract was added to drinking water as 800 PPm concentration. They, systolic blood pressure and renal blood flow (RBF) were measured. Also, the amounts of urea, creatinine, sodium, potassium and osmolarity were measured in plasma and urine samples In co-treatment group with zataria Multiflora extract, the amounts of urea, creatinine, absolute sodium excretion and relative sodium and potassium excretion and malondialdehyde (MDA) that have been inceased in treatment with gentamicin, significantly were reduced. Creatinine clearance, urine osmolarity, RBF and FRAP that was decreased in gentamicin group in compare to control group, significantly increased. Co-treatment prevents nephrotoxicity induced by gentamicin and attenuates oxidative-stress associated renal injury by reducing oxygen free radicals and lipid peroxidation, So it can be effective to cure rats receiving gentamicin.

Q fever is a zoonotic agent that is endemic in the many parts of the World. It has animal origin as considered as an emerging and re-emerging zoonose in many countries, including Iran. Cattle, sheep, and goats are the primary reservoirs for Q fever. Organisms are excreted in milk of infected animals. This study was conducted to determine the prevalence rate of Coxiella burentii in raw samples obtained from sheep in Bonab. This study was carried out from Autuman 2014 to Winter 2014. Overall, 120 milk samples were collected from 100 dairy cattle breeding complexes and the diagnosis of Coxiella burnetii was confirmed by Nested-PCR method. In this study, in total, 26 samples (21.66%) were found to be positive for the presence of Coxiella burnetii. Considering the importance of the bacterium, Coxiella burnetii, rapid and accurate diagnosis is of great significance. Due to high accuracy and high speed, molecular techniques are mostly effective in the diagnosis. Thus, the localization of molecular techniques in the diagnosis of Q fever is highly recommended. The results indicated that Cattle's milk could be a potential reservoir of Coxiella burnetii in Iran.

Brucellosis is a highly contagious zoonotic disease between humans and animals known for high frequency of relapsing and ability to cause chronic infection. The function of immune system plays an important role in induction of chronic diseases. However, the role of immune system response is not completely studied. Therefore, this study designed to investigate the cytokine profile of the patients suffering from chronic and acute brucellosis. This descriptive- analytical study was performed on 22 patients with acute brucellosis (mean age 38±17), 21 patients with chronic brucellosis (mean age 43±10) and 21 healthy people (mean age 26±4) with the same age and sex as patients. The serum IFN-γ, IL-17, IL-5 and TGF- β levels were measured using ELISA method. The mean IFN-γ serum level in acute and chronic brucellosis patients group was significantly higher than control group (p=0.045). The mean IL-17 serum level in acute brucellosis patients was significant decreased once compared with control group and in chronic patients was significantly decreased when compared with control group (p=0.024). In addition, the mean IL-5 and TGF-β serum levels of acute brucellosis patients group were significantly decreased as compared to chronic patients (p=0.001). The results of current study indicate that cytokine profile of chronic brucellosis patients is more related to Th2 immune response. Hence, Th2 immune response inhibition would be an appropriate way to treat and prevent disease to become chronic.

Cervical cancer is the third most common genital malignancy in women. In majority of developing countries the prevalence of this malignancy is high. The aim of this study was to determine the prevalence of cervical cancer among married wemon in Arak. This cross-sectional and analytical study was carried out on 1000 married women. Demographic variables involving age, literacy, history of hysterectomy, nutrition statute, marriage age, history of abortion, smoking habits, etc were filled by questioners. Then Pap smear was done in all subjects and specimens were sent to the pathobiology laboratory. The data were analyzed with SPSS statistical software. The mean age of studied subjects was 39.38±10.1, the most freqrency of marriage once was 459(45%), the mean age of marriage was17.9±10.3, the mean age of first sexual intercourse was 15.1 (9.2%), the most frequency of the numbers of peregnancy was 2.6±2.1, and the mean times of abortion once was 153(15.3%). In this study, the prevalence of cervical cancer was 0.004(4 per 1000). There was a significant correlation between age, hysterectomy the time of marriage and painful sextual intercourse and cervical cancer (p<0.005). There was no statistically significant relation between smoking, food diet, pregnancy prevention drugs and the other variables of the study of cervical cancer (p>0.005). With attention to high prevalence of cervical cancer, promoting education and knowledge about the importance and necessity of pap smear and risk factors of cervical cancer and encouraging marriaged women to take part in screening programs is necessary.

Aluminum, as an environmental pollutant, has destructive effects by inducing oxidative stress on male reproductive system and sperm. Silymarin, an effective substance extracted from Silybum marianum, is a potent antioxidant which inhibits oxidative stress. Because of toxic effects of aluminum and the antioxidant role of silymarin, this study was performed to investigate if silymarin can prevent the adverse effects of aluminum chloride on plasma membrane integrity and acrosome integrity in ram sperm. In this experimental study, epididymal spermatozoa from Farahani's ram are divided into five groups: sperm at 0 hour, sperm at 180 minutes (control), sperm treated with aluminum chloride (0.5mM) for 180 minutes, sperm treated with silymarin (0.5μM) + aluminum chloride (0.5μM) for 180 minutes and sperm treated with silymarin (0.5μM) for 180 minutes. To evaluate sperm plasma membrane integrity and sperm acrosome integrity, propidium iodide-Hoechst and comassie brilliant blue staining were used, respectively. The results were analyzed using one-way ANOVA and p<0.05 was considered as significant level. The percentage of sperm plasma membrane integrity and acrosome integrity were significantly decreased in aluminum chloride group compared to the control. The simultaneous use of silymarin+aluminum chloride could significantly compensate the adverse effects of aluminum chloride on the sperm plasma membrane integrity and acrosome integrity compared to aluminum chloride. Aluminum chloride induces toxic effect on ram sperm plasma membrane integrity and acrosome integrity and silymarin is able to compensate the adverse effect this pollutant on these parameters.

Medicinal plants are primery source of many drugs to cure different diseases. The genus Prangos, (Umbelliferae family) consists of several medicinal plants that their desirable dffects have been approved. The aim of this study is to investigate the effectiveness of methanol extract in different parts of prangos ferulacea and prangos acaualis on human lymphocytes proliferation and their mutagenicity in salmonella typhimurium TA98. In this experimental study, the plants were collected from different areas of Kurdistan. Then, samples were air dried and powdered and methanol material of plants was extracted. The extracts were diluted to give concentrations of 10, 100, 500, 1000, 1500, 2000, 2500 μg/ml. Finally, the effects of these extracts on human lymphocytes proliferation and their mutagenecity have been investigated by the MTT and Ames test. The results showed that different organs extract from both tested plants caused a significant increase in lymphocytes proliferation, specially in concentrations of 500 to 2500 μg/ml. Of studied excrtacts, the highest and lowest effect on lymphocytes proliferation was obtained in presence of flower and seed, respectively. In total, the levels of proliferation resulted of prangos ferulacea as compared with prangos acaulis were higher. Also, the results of study showed no mutagenicity of studied plant exctracts with considered concentrations. The findings revealed that both species of prangos can increase immune system function and were used as an safe medicinal plant to cure patients with immune deficiencies and microbial infections.