فهرست مطالب

Researcher Bulletin of Medical Sciences
Volume:20 Issue: 1, 2015

تاریخ انتشار: 1394/06/20
تعداد عناوین: 9

فارسی
English

انتخاب همه

Investigating the relationship between the level of CRP with PPROM and cervical length in pregnant women who visit in the prenatal care clinic of Dr. Shariati hospital of Tehran

Roghaye Hosseinian Kandsar, Lale Eslamian, Vajihe Marsoosi Pages 1-4

Background And Aim
PPROM is defined as the preterm premature rupture of membranes before the initiation of delivery and before the 37th week of pregnancy. PPROM has many maternal and fetal complications and is primarily generated by infection. This infection can bring about changes in cervical length at the incipience of pregnancy. Since CRP levels increase in blood in response to infection and inflammation, the aim of this study is to investigate the relation between mothers’ CRP level as a marker of infection with cervical length and the occurrence of PPROM.
Materials And Methods
418 pregnant women with singletons who had visited the parental clinic of Shariati hospital participated in this descriptive study. Their CRP level was measured based on mg/dl between the 6th and 20th weeks of gestation and their cervical length was specified with vaginal ultrasound based on mm between 14 to 20 weeks of pregnancy. In this study the relation between the CRP level and the incidence of PPROM with cervical length was statistically determined via the t-test.
Results
Out of 418 pregnant women 11 (2.6 percent) had PPROM and their CRP level was 22.5 mg/dl±2.8. The remaining 407 people who didnt suffer from PPROM had a CRP level of 8.73 mg/dl±4.5. In this research there was a significant relation between CRP level during the first 20 weeks of gestation and the incidence of PPROM (P<0.000). Furthermore, the CRP level in people who had a cervical length of 25mm or less was 21mg/dl±4.8. It was 8.5mg/dl4.2 in people who had a cervical length of greater than 25mm. The study found a significant relation between the mother's CRP level with a cervical length of 25mm or less during the first 20 weeks of pregnancy (P<0.000).
Conclusion
It seems that the CRP level is associated with the incidence of PPROM and cervical length and that the CRP level as a marker of inflammation and infection is higher in people affected with PPROM and those who have a cervical length of 25mm or less. To investigate this further, it’s recommended that a cohort research be studied.
Keywords: Preterm Premature Rupture of Membranes (PPROM), C, Reactive Protein (CRP), Cervical length (CL)

Abstract View Paper Original: Persian
The comparison of planning and information processing speed in multiple sclerosis patients and non-patients according to levels of depression, anxiety and stress

Mahdieh Azizpour, Sepideh Ragezi Esfahani, Gita Afroukhte, Fahimeh Akhoundi, Jamal Shams Pages 5-11

Background And Aim
As for the importance of ability to plan and the speed of processing information in patients with multiple sclerosis and the role of other factors such as depression, anxiety and stress in the performance of this research was conducted in 92 centers in Tehran MS Society.
Materials And Methods
This study is based on historical homogeneity and was conducted on 54 patients with the diagnosis of relapsing multiple sclerosis and 54 controls. DASS-2 questionnaire and computerized Tower of London test were administered among selected samples.
Results
Results show significant difference on planning and processing information speed between the two groups. There were differences on cognitive function with considering the level of depression, anxiety and stress.
Conclusion
MS disease can be resulted to impairment in sustained planning and speed of processing information. Moreover, depression anxiety and stress intensify these cognitive impairments.
Keywords: Multiple sclerosis, Planning, information processing, Depression, Anxiety, Stress

Abstract View Paper Original: Persian
Relationship between spiritual well-being and cortisol level in nursing students

Davoud Pourmarzi, Seyedeh Maryam Mosavi, Shady Dehganzadeh, Babak Ruzbehan, Golzar Pourhamed Pages 12-17

Background And Aim
High level of cortisol that is concentrated due to stress expose had negative effects on health. Spiritual well-being cause positive fall and can decreases cortisol level. This study aimed to determine relationship between spiritual well-being score and cortisol level in nursing students.
Materials And Methods
This was a descriptive analytical cross-sectional study that was carried out on 100 nursing students in Rasht Islamic Azad University who were training at internship in 2013. Simple random sampling based on students’ identification number was performed. We used standard spiritual well-being (SWB). Serum cortisol level was obtained at morning between 10 and 12. For statistical analysis we used independent t-test and Pearson’s coefficient of correlation.
Results
Mean of spiritual well-being and its religious and existential well-being scale were 97.8±13.4, 48.6±7.1, and 49.2±7.2, respectively. Most of students (51%) were in high level of Spiritual well-being and rests (49%) were in moderate level. Mean of plasma cortisol level was 14.08±5.08. There was no significant correlation between spiritual well-being and its subscales with serum cortisol level. Among 20 questions in Spiritual well-being scale there were significant negative correlations between cortisol level and “I believe that God is concerned about my problems” (r=-0.211, P=0.035) and “I don’t enjoy much about life” (r=-0.202, P=0.044).
Conclusion
There was no relationship between serum cortisol level in morning and spiritual well-being scale and its subscales. However, the belief that God is a helper in problems and enjoy about life have relations with low level of serum cortisol.
Keywords: Spiritual well, being, Cortisol, Stress, Nursing students

Abstract View Paper Original: Persian
The induction of amniotic fluid mesenchymal stem cells to ocular lens fiber cells

Nasim Eslami, Homa Mohseni Kouchesfehani, Mohammad Nabiuni, Parisa Gheibi, Khadijeh Bahrebar Pages 18-25

Background And Aim
Mesenchymal stem cells (MSCs) are undifferentiated cells with ability of high proliferation that can differentiate into cells derived from mesenchymal and non-mesenchymal tissues. The aim of this study is isolation of amniotic fluid MSCs and induction of them to differentiate and produce lens fiber cells.
Materials And Methods
Amniotic fluid MSCs was isolated from female pregnant mice and cultured with DMEM, FBS and antibiotic. MSCs, which were passaged three times, were cultured with DMEM, FBS, antibiotic and vitreous body for 14 days to induce the differentiation. MSCs and differentiated cells were identified by morphological observation and immunocytochemistery studies.
Results
Amniotic fluid MSCs expressed Oct4. Cultivated cells with vitreous body were locally longer and more parallel aligned with more nucleoli compared to control group cells. The expression of crystallins in treated cells was demonstrated with immunocytochemical studies.
Conclusion
Amniotic fluid MSCs can differentiate in to lens fiber cells. Therefore, differentiated cells can be used to cure eye disease.
Keywords: Amniotic

Abstract View Paper Original: Persian
Low HDL prevalence in patients with acute coronary syndrome admitted in Loghman Hakim hospital

Maryam Taherkhani, Hamed Asadi, Adineh Taherkhani Pages 26-32

Background And Aim
Cardiovascular disorders especially coronary artery diseases are the most common causes of death in the developed and developing countries. According to large studies low HDL is an independent predictor of coronary artery diseases. Its cost effective to treat low HDL compare with coronary diseases. The aim of this study is determining the prevalence and causes of low HDL in patients with acute coronary syndrome.
Materials And Methods
This was a cross sectional study. 300 patients who admitted in CCU of Loghman Hakim hospital because of acute coronary syndrome enrolled in our study. These parameters surveyed in this study: plasma level of total cholesterol, triglyceride, high density lipoprotein, low density lipoprotein, age, sex, history of HTN, DM, IHD, CVA, renal and hepatic disease. The information was analyzed with chi-square test and t-test.
Results
HDL average in women was 50 mg/dl and in men was 48 mg/dl. TG average in men was 180 mg/dl and in women was 183 mg/dl and cholesterol average in men was 196 mg/dl and in women was 182 mg/dl. Thus, there was not a significant association between two sexes. There were no patients with HDL lower than 25 mg/dl. 80 patients (26.7%) had HDL lower than 40 mg/dl and 54 patients (18%) had HDL more than 60 mg/dl. In 1% of patients low HDL was the unique risk factor of IHD. Between hypercholesterolemia and low HDL with p-value of <0.03 and between high LDL and low HDL with p-value of <0.05 were significant association. There was no significant association between low HDL in patients with and without HTN and patients with and without DM. There was significant association between smoking and low HDL (p-value<0.024). Total cholesterol/HDL index in all patients was 4 over 1. DM, HTN and smoking have no significant effect on this index. Low HDL was the most important factor that affects this index and there was significant association between low HDL and total cholesterol/HDL index (P<0.05).
Conclusion
About one third of patients who was admitted with acute coronary syndrome had HDL less than 40 mg/dl. Although determining independent role of low HDL in our study was not possible but it needs more concern.
Keywords: HDL, Ischemic heart disease, Dyslipidemia

Abstract View Paper Original: Persian
The occurrence of TEM gene among the extended-spectrum β-lactamases (ESBLs) producing uropathogenic Escherichia coli strains, isolated from Khorramabad city in 2013

Gholamreza Goudarzi, Azin Baharvand Ahmadi Pages 33-37

Background And Aim
The extended-spectrum β-lactamases (ESBLs) are mutant enzyme variants, which primarily derived from broad-spectrum β-lactamases TEM 1, 2 and SHV1. They have a wide substrate profile, allowing hydrolysis of all cephalosporins, penicillins and aztreonam. There is remarkable geographical diversity in the occurrence of ESBLs in Iran. The current study was investigated to look for the presence of TEM gene among ESBLs-positive uropathogenic Escherichia coli (UPEC) isolates in Khorramabad city, Iran in 2013.
Materials And Methods
In this cross-sectional study, a total of two–hundred uropathogenic E. coli isolates were collected and identified from selected hospitals of Khorramabad city. Screening and confirmation of ESBL production was phenotypically carried out according to the Clinical and Laboratory Standards Institute (CLSI) recommended methods. The frequency of TEM gene among ESBLs-positive isolates was determined using PCR technique.
Results
Out of 200 uropathogenic E. coli, 91 (45.5%) isolates were shown to produce ESBLs as investigated by disk diffusion test, which among them, 60 (65.9%) isolates carried TEM genes as detected by PCR.
Conclusion
The frequency of ESBL-positive UPEC isolates, as well as harboring TEM is high in Khorramabad city. This subject is a serious threat. Therefore, it may be resulted in the treatment failure. In addition, at least in our region, carbapenems (e.g. imipenem) can be as an antibiotic choice to eradicate ESBLs-producing UPEC isolates. We recommend the hospitals to perform laboratory methods for detection ESBLs-positive isolates, as well as antibiotic prescription policy, particularly in cases of empirical therapy to be revised.
Keywords: PCR_Escherichia coli_TEM_Extended_spectrum β lactamases

Abstract View Paper Original: Persian
Typing of E. coli isolated from influent of Tehran province treatment plants surface water by ERIC-PCR

Abbas Akhavan Sepahi, Kambiz Davari, Leili Akhtari Pages 38-44

Background And Aim
Typing of bacterial strains is used for epidemiologic studies. Here we investigated the distribution of ERIC elements in the complete genome sequences of Escherichia coli (E. coli) strains. The aim of the current study was to apply ERIC-PCR method for molecular typing of E. coli strains isolated from influent of Tehran province surface water treatment plants by ERIC-PCR.
Materials And Methods
Current study included E. coli strains all isolated from 5 Tehran province surface water treatment plants. Bacterial isolates were all detected and identified by standard microbiological and biochemical tests and genomic fingerprints and relationship between the strains was evaluated by ERIC-PCR using specific primers followed by UPGMA clustering method applying online in silico tool.
Results
One hundred and six E. coli isolates were collected from Tehran surface water sources. Applying ERIC-PCR, all strains were typeable, since 32 different bands ranging from 163 to 3200 bp were amplified in different profiles. Following dendogram analysis, ERIC-PCR could categorize the strains within 9 ERIC clusters.
Conclusion
The results of current study confirmed that E. coli strains isolated from different treatment plants of Tehran province belong to diverse clones and different genotypes and could not be divided in any specific clusters. However, ERIC-PCR is a powerful molecular tool with high performance and good discriminatory power. Keywords: Keywords:
Keywords: Genotyping, Genomic finger print, E. coli, ERIC

Abstract View Paper Original: Persian
Characterization of macrolide resistance in Streptococcus pyogenes: prevalence, phenotype and genotype

Fahimeh Baghbani, Arani, Khoroosh Javan, Kambiz Davari Pages 45-50

Background And Aim
The increase in macrolide resistance among Streptococcus pyogenes isolates is a cause to be concerned. To investigate the macrolide resistance trends, phenotypes and genotypes, we examined 40 clinical isolates of Group A Streptococcus (GAS) collected between 2013 and 2014.
Materials And Methods
Susceptibilities to macrolide antimicrobial agents were determined by disk diffusion method and inducible clindamycin resistance was identified using D-zone test. Beside, macrolide resistance genes were detected by polymerase chain reaction (PCR).
Results
The rates of resistance to erythromycin, azithromycin, clindamycin and clarithromycin were 20%, 20%, 15%, 10% respectively. None of the isolates exhibited resistance to penicillin. Among 8 erythromycin-resistant Streptococcus pyogenes isolates 4 had an M phenotype and 4 had an MLSB phenotype (3 cMLSB and 1 iMLSB). The isolates with cMLSB and iMLSB phenotype had ermA and ermB gene, respectively. Of 16 macrolide-resistant GAS isolates, 5 (31%) harbored the ermB gene and 1 (6%) the ermA. Also, one isolate without any macrolide resistance was positive for ermB gene.
Conclusion
We found a high level of macrolide resistance in Streptococcus pyogenes in Iran. Moreover, clindamycin and clarithromycin would be the drugs of choice for treating penicillin-allergic patients. The phenotypes of erythromycin resistance seem to be associated with certain erm genotypes in Streptococcus pyogenes isolates.
Keywords: Streptococcus pyogenes, erm gene, Macrolide resistance, PCR

Abstract View Paper Original: Persian

علمی مصوب

Researcher Bulletin of Medical Sciences

مجله بولتن پژوهش در علوم پزشکی

سالنامه پزشکی

آخرین شماره | آرشیو

ISSN: 1735-1022 eISSN: 2008-7780

تا سال 96 با نام نشریه «پژوهنده» و با زبان فارسی منتشر شده است.

صاحب امتیاز:

دانشگاه علوم پزشکی شهید بهشتی

مدیر مسئول:

دکتر سید محمود طباطبایی فر

سردبیر:

دکتر علی حائری

تلفن نشریه: ۰۲۱-۲۲۴۳۹۷۸۳

اطلاعات بیشتر نشریه

درباره نشریه پیام به نشریه سایت اختصاصی نشریه پذیرش الکترونیکی مقاله راهنمای نویسندگان

به جمع مشترکان مگیران بپیوندید!

فهرست مطالب

Researcher Bulletin of Medical Sciences Volume:20 Issue: 1, 2015

Researcher Bulletin of Medical Sciences

مجله بولتن پژوهش در علوم پزشکی

Researcher Bulletin of Medical Sciences
Volume:20 Issue: 1, 2015