Effect of NaCl on morphological characters, protein profile and chlorophyll content of wheat cultivar.

Salinity stress negatively affect agricultural yield throughout the world. The aim of this study، which was done in Arak university during the year 2009، was to investigative the effect of salt stress on morphology، chlorophyll content and protein profile of wheat cultivar “shahriar”. Wheat was grown on Hoagland media containing 0، 25، 50، 75 and 100 mM of NaCl for a period of 15 day، then the length and weight of the leaf and root was measured every 3 days and samples were collected for further analysis. Morphological study was carried on a tine section prepared using crayostat under an olympous light microscope. Chlorophyll content of the 80% acetone extracted samples was estimated using Brouers method. Samples were extracted and run on 12% polyacrylamid gel with the help of constant voltage. Salt stress resulted in 44 and 43% reduction in weight of leaf and root respectively as compared to control. In addition significant reduction of chlorophyll content in salt treated samples were observed followed by morphological changes in vascular tissue. There were 15 major polypeptide/proteins bands observed in the control group، where at least changes in 5 bands could be observed due to salt stress in samples treated with NaCl. The result of this study showed that the salinity caused morphologica، chlorophyll content as well as protein profile changes in shahriar cultivar.