Effect of Different Concentrations of Growth Regulators on Shoot Regeneration and Proliferation in Strawberry (Fragaria ananassa Duch.) Cultivars

This study was carried out for shoot regeneration and proliferation of three strawberry cultivars runner tip. Sterile runner tips’ having terminal buds (4-5 mm) of Kurdistan, Parose and Camarosa cultivars were cultured onto MS media with different concentrations (0.25, 0.5, 1 and 2 mg/l) of benzyl adenine (BA) alone or supplemented with IBA (0.1, 0.25 and 0.5 mg/l) growth regulator for the above purpose. The type and concentration of growth regulators and cultivar were found critical to shoot regeneration. The highest response of shoot multiplication was obtained in MS containing 1 mg/l BA + 0.25 mg/l IBA. This combination yielded the highest shoot number and percent of regeneration from Kurdistan (13.13 shoots and 92.33% regeneration), Parose (10.76 shoots and 89.33% regeneration) and Camarosa (12.15 and 80.16%) cultivars. For proliferation, four-week-old shoots were removed from the culture, and subcultured on MS basal medium supplemented with various levels of BA (0.25, 0.5 and 1 mg/l) alone or in combination with GA (0.0, 0.25 and 0.5 mg/l). Best response towards multiple shoot regeneration (Kurdistan 34.47, Parose 28.46 and Camarosa 30.25 shoots) was obtained on MS medium having 0.5 mg/l BA. While the highest harvestable shoots was recorded (Kurdistan 21.32, Parose 17.84 and Camarosa 18.59 shoots) in MS containing 0.5 mg/l BA + 0.25 mg/l GA.