The effects of diesel engine exhaust on development Carnation (Dianthus caryophyllus L) in tissue culture conditions

Dianthus caryophyllus L. is one of the most importantflower products and cut branches plants. In severral recent decades its qualitative and quantitative traits have received more attention of gardening. researchers Using tissue culture techniques is a usefal selecting method for modification and improvement the qualitative traits of this plant. in the view of the fact that in community urban the effect of diesel exhaust particles demonstrated on plant growth، based on this case our purpose of this research is to study the effect of different concentration of diesel exhaust particles on the growth and some physiological activities of Dianthus caryophyllus L. for this reason in a completely random plan، Sterile base of Dianthus caryophyllus L. were، divided into groups treatment and control in the base medium MS and we placed them into 8 groups. Then the plants placed under the treatment of diesel exhaust particles with concentrations (1،2/5،5،7/5،9،10،12) mg/L. The results of microscopic studies of growth factors and physiologic activities showed that increasing diesel exhaust particles cause decreasing in growth factors and changing physiologic activities. the results obtained about some growth factors such as dried and fresh weights Leaf area NAR، BMD in the probability level 1% or5% were significant and in some factors such as leaf number wasn،t significant. The result of physiological activities also showed that the plant by increasing diesel exhaust particle cause significant increasing proline amino acid in the plant.