Effect of Conditioned Medium of Human Mesenchymal Stem Cells on Acute Renal Failure Induced by Cisplatin in Rat

Cisplatin is one of the most successful drugs to fight many cancers، but its nephrotoxicity limits its administration. The aim of this study is investigation of the effect of human mesenchymal stem cells secretory factors on Cisplatin-induced acute renal failure in rats. In this study، male wistar rats were used. Animals were randomly divided into four groups and six animals were in each group. One group was considered as control which this group received no injections. Other three groups were treated with intraperitoneal 5 mg/kg Cisplatin once at the beginning of the experiment. Then one of these three groups was injected with conditioned medium of human mesenchymal stem cells and other groups were injected with medium without secretory factors intraperitoneally for three consecutive days. After five days Cisplatin injection، kidney and blood samples from all of examined animals were collected for histological and biochemical studies. Results showed significant decrease in the kidney injury level، blood serum BUN and creatinine amount. Also Also body weight increasing significantly was observed in conditioned medium of human mesenchymal stem cells receiver group in comparison with treated Cisplatin and non secretory factors receiver groups. Results of this research suggest that human mesenchymal stem cells secretory factors can be affect against Cisplatin-induced nephrotoxicity.