The protective effect of vitamin E and selenium on liver tissue in fructose- induced diabetic rat

Diabetes disorder in organ's function via oxidative stress. In this research, the protective effect of vitamin E and selenium "as antioxidant" on liver function were investigeted in diabetic rats. 35 adult male rats (Wistar) weighing 180 ± 20g divided randomly into control, sham and 3 experimental groups (E, S and E+S). All of groups except control were fed 140 cc fructose 10% as a daily for 9 weeks. Control group without treatment, sham were injected 0.20 cc of physiologic serum and and group (S) were injected 0.20 cc of selenium solution (0.5 mg/kg/b.wt) intraperitoneally. group (E) were gavaged 0.20 cc of vitamin E solution (0.5 mg/kg/b.wt) and Group (E + S) gavaged vitamin E and were injected selenium (0.5mg/kg/b.wt) for 9 weeks. Blood sampling of all groups and were separated for biochemical analyzes. Then liver was disected and fixed in 3% formalin. hbafty Automatic Tissue processor with 5 micrometer and were examined by light microscope. The sections with 5 micron thickness were prepared by microtome after passage in Tissue processor automatically. They were studied on light microscope. Using statistical software, SPSS 17, data were analyzed by ANOVA and groups were compared by Touki test at a significance level (P <0.01) were compared. Fingings: The mean levels of Aspartataminotransfrase, Alkalinphosphat and blood glucose concentrations in sham group were increased compared to control and in treatments groups, especially (E + S) decreased significantly(p<0.01). Histological study showed the changes as hydropic cytoplasm, lymphocytic invasion around the portal triad and necrosis in the sham group but none observed in the treatment groups, especially group(E+S). These results show that vitamin E and selenium play a crucial protective role against diabetes type II and using them simultaneously, due to synergic effect, better result obtaine. Vitamin E and selenium compensate partially the changes caused by diabete II and the combined use of them have better operation via synergistic effect.