Effect of Conditioned Media from Human Mesenchymal Stem Cells on Acute Kidney Injury Induced by Gentamicin in Rat

The aim of this study was to investigate the effect of secretory factors of human mesenchymal stem cells on gentamicin-induced acute kidney injury in rats. Firstly, an experiment was designed to achieve a standard animal model. Animals were randomly divided into four groups. One group was considered as control which received no injection. Three other groups were treated with intraperitoneal 100 mg/kg gentamicin for six consecutive days. Then one of the three groups was injucted with conditioned media of human mesenchymal stem cells and other group was injucted with medium without secretory factors intraperitoneally for three consecutive days. Finally, in the third, fifth and eighth days after gentamicin injection, kidney and blood samples from all animals were collected for histological and biochemical analysis. Results, except in the special case, showed no significant decrease in the amount of blood biomarkers of animals in the group receiving the condition media of human mesenchymal stem cells compared to two another groups. The improvement of tissue damage, however, has been observed in the number of days. These findings suggest that secretory factors of human mesenchymal stem cells have aprotective effect against gentamicin-induced nephrotoxicity