Effect of Basic Fibroblast Growth Factor on in Vitro Maturation of Oocytes of Mouse at the Stage of Germinal Vesicle

In vitro maturation (IVM) of oocytes, providing oocytes maturation out of normal conditions, is an appropriate infertility treatment system, though the clinical use of IVM is limited due to low rate of success. Accordingly, this study aimed to analyze the effect of fibroblast growth factor on in vitro maturation of immature oocytes.

Immature oocytes of 20 female mice of NMRI strain aged 8-10 weeks were obtained 46-48 hours after intraperitoneal injection of 10 units of Pregnant Mare`s Serum Gonadotrophin (PMSG). The oocytes were treated within Modified Essential Medium (MEM-α) supplemented with 0 ng/ml, 10 ng/ml, 20 ng/ml and 40 ng/ml doses of fibroblast growth factor respectively. After 24 hours, Oocyte maturation stage was scrutinized by an invert microscope and its growth rate was analyzed via SPSS software utilizing ANOVA test.

The resumption percentage of meiosis was reported as 23 in the first control group, while it was 25.7, 26.2, 27.3 % respectively for the second, third and fourth experimental groups; thus, no significant differences was observed among control groups and experimental groups. Yet in vitro maturation of the control group, a significant difference was observed compared to those of the second and third experimental groups (p<0.01). In fact, the rate of vitro metaphase matured oocytes were reported as 45, 60.8, 62.6 and 45.2 % respectively in the control group and the second, third, and fourth experimental groups.

The obtained results of study illustrated that 10 ng/ml and 20 ng/ml concentrations of fibroblast growth factor have a major impact on resumption of meiosis, nucleus break down and extrusion of the first polar body, whereas the effect of 40 mg/ml concentration on improvement of oocyte maturation was trivial.