Synthesis Nano ZnO Assisted by Ultrasound Irradiation and Evaluation of Antimicrobial Properties

Introducing a powerful antibacterial agent to control pathogenic bacteria especially strains resistant to antibiotics is of paramount importance. The purpose of this study is ZnO nanoparticles synthesis by using ultrasonic waves and evaluation of its antibacterial properties. Zinc oxide nanoparticles were synthesized using ultrasound irradiation. Then physical and chemical properties of nanoparticles were studied. Antibacterial activity of the nanoparticles was investigated for two strains bacteria: Escherichia coli and Staphylococcus aureus through standard methods such as placing well and disc diffusions, minimum inhibitory concentration (MIC), and minimum bactericidal concentration (MBC). Chemical and physical investigation of the synthesized ZnO nanoparticles indicated that from nanoscale particles, spherical forms were produced with no impurities. The MIC for E.coli and Staph.aureus were reported as 0.125 mg/ml, and 0.062 mg/ml respectively. The MBC for E.coli and Staph. aureus was reported to be 0.500 mg/ml, and 0.250 mg/ml respectively. In comparison, for the methods of well and disc diffusions, inhibition zone diameter of the well method was more than disc diffusion. High purity zinc oxide nanoparticles can be synthesized by ultrasonic waves and have the significant antibacterial properties against Escherichia coli and Staphylococcus bacteria. In contrast to Escherichia coli, Staph. aureus showed greater sensitivity against Zinc Oxide nanoparticles.