Biochemical compositions of ovarian fluid of Rutilus frisii kutum (Kamensky, 1901) in the south Caspian Sea and their effects on spermatozoa motility traits

Some of index of ovarian fluid biochemical such as ionic، organic and their relationships with osmolality in 10 specimens was investigated. Also spermatozoa motility of Rutilus frisii kutum was investigated in different percentage (0، 33، 50، 67، 75، 80 and 85%) of ovarian fluid. ovarian fluid contained 98. 35±14. 33 mmol/l Na+، 7. 22±1. 81 mmol/l Mg+، 8. 08±2. 29 mmol/l K+، 619. 69±171. 60 mg/dl cholesterol، 9. 76±2. 64 mg/dl Ca2+، 13. 55±4. 15 mg/dl protein، 359. 43±70. 26 mg/dl glugose. The range of pH was 7. 71±0. 4 and the range of osmolality was 237. 57±22. 10 mmol/Kg. When Rutilus frisii kutum semen diluted with 33 and 50% ovarian fluid، the spermatozoa had highest motility. The total duration of motility and percentage of motile spermatozoa was greatly raduced when semen was diluted of ovarian fluid higher than 50%. Generally sperm motility of Rutilus frisii kutum affected by high concentrations of Na، osmolality and glugose.