The Effect of Extraction Method on Bioactive Phenolic Compounds of Cupressus arizonica

The effect of Soxhlet and simple immersion extraction methods on the amount and type of bioactive phenolic compounds of C. arizonica woodknots were studied. Lipophilic and hydrophilic compounds were Soxhlet extracted according to T280 pm-99 standard method with hexane and acetone solvents, respectively. Also, in the immersion method woodknot samples were dipped in hexane to remove the lipophilic moieties and then treated with ethanol: H2O (1: 9, v/v) solvent to isolate the hydrophilic compounds. Also, an alkaline hydrolysis step with one molar NaOH solution was performed to remove sugar impurities from the ethanolic extract of the immersion method. GC-MS was used to identify the extractives components of different procedures. The results of analysis indicated the efficiency of bioactive components extraction with ethanol: H2O (1: 9, v/v) solvent comparing to acetone procedure. Moreover, the simple immersion method was showed to be a suitable method in extraction of bioactive extractives. Contribution of matairesinol (MR), dienestrol and curumine in the extract of EtOH: H2O were 11.2, 0.4 and 0.9%, respectively. Alkaline hydrolysis led to a partial degradation of phenolics according to the results of GC-MS analysis.